CS265509B1 - A method of producing chemically pure L-lysine - Google Patents
A method of producing chemically pure L-lysine Download PDFInfo
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- CS265509B1 CS265509B1 CS862922A CS292286A CS265509B1 CS 265509 B1 CS265509 B1 CS 265509B1 CS 862922 A CS862922 A CS 862922A CS 292286 A CS292286 A CS 292286A CS 265509 B1 CS265509 B1 CS 265509B1
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Abstract
Riešenie sa týká spůsobu výroby Llizýnu. Technický L-lyzín sa odsolí a připraví sa roztok L-lyzínu o vodivosti 200 až 1 000 ,4tS. cm-l. Roztok sa autofokusuje jednosměrným elektrickým prúdom o napatí 200 až 1 000 V po dobu 24 až 48 hodin. Vytvára sa gradient pH, pozdlž ktorého sa příměsi L-lyzínu a čistý Llyzín fokusuji! podlá svojich izoelektrických bodov. Roztok tvoriaci frakciu o pH 9,2 až 10,0 sa aspoň jedenkrát refokusuje a z frakcií, s výhodou o pH 9,6 až 9,8, sa získá dehydratáciou chemicky čistý L-lyzín.The solution relates to a method of producing L-lysine. Technical L-lysine is desalted and a solution of L-lysine with a conductivity of 200 to 1,000.4tS. cm-1 is prepared. The solution is autofocused with a direct electric current of 200 to 1,000 V for 24 to 48 hours. A pH gradient is created along which L-lysine is added and pure L-lysine is focused according to its isoelectric points. The solution forming a fraction with a pH of 9.2 to 10.0 is refocused at least once and chemically pure L-lysine is obtained from the fractions, preferably with a pH of 9.6 to 9.8, by dehydration.
Description
Vynález sa týká výroby chemicky čistého L-lyzínu zo základnej suroviny technického L-lyzínu.The invention relates to the production of chemically pure L-lysine from the basic raw material of technical L-lysine.
Doterajšia výroba L-lyzínu vo velkom sa realizovala biosyntézou mikroorganizmami rodu Corynebacteriaceae. Z pódy, do ktorej baktérie produkují L-lyzín sa vo vSčšine prípadov izoláciou získává jednak technický lyzín s obsahom do 86 % L-lyzínu v zmesi aminokyselin, alebo dalšou nákladnou purifikáciou chemicky čistý L-lyzín s obsahom až 98 % čistej aminokyseliny.The large-scale production of L-lysine so far has been accomplished by biosynthesis of microorganisms of the genus Corynebacteriaceae. In most cases, isolation yields either technical lysine containing up to 86% L-lysine in a mixture of amino acids, or further expensive purification of chemically pure L-lysine containing up to 98% pure amino acid from the source to which the bacteria produce L-lysine.
Společnou nevýhodou doterajších spósobov výroby bola zložitá manipulácia, vysoké náklady na zariadenie a energiu.The common disadvantage of the existing production methods was the complex handling, high equipment and energy costs.
Uvedené nevýhody v podstatnej miere odstraňuje spósob výroby chemicky čistého L-lyzínu podlá vynálezu, ktorého podstata spočívá v tom, že sa L-lyzín získaný biosyntézou a izolovaný o technickej čistotě rozpustí v destilovanej vodě v takej koncentrácii, aby mal roztok vodivost v rozmedzí 200 až 2 000 ,uS.cm_1, výhodné 800^.uS.cm-1. Roztok sa autofokusuje jednosměrným prúdom o napatí 200 až 1 000 V počas 24 až 48 hodin. Frakcie s pH blízkým izoelektrickému bodu L-lyzínu, výhodné od 9,2 do 10, obsahujúce až 96 i L-lyzínu se refokusujú a po refokusácii vo frakciách pri pH 9,6 až 9,8 je obsah L-lyzínu aspoň 99 % v chemicky čistej formě.The above-mentioned disadvantages are substantially eliminated by the process for producing chemically pure L-lysine according to the invention, which consists in dissolving the L-lysine obtained by biosynthesis and isolated in technical purity in distilled water in a concentration such that the solution has a conductivity of 200 to 200. 2000, uS.cm _1, preferably 800 .uS.cm ^ -1. The solution is autofocused with a direct current of 200-1000 volts for 24-48 hours. Fractions with a pH close to the isoelectric point of L-lysine, preferably from 9.2 to 10, containing up to 96 i L-lysine are refocused and after refocusing in fractions at pH 9.6 to 9.8, the L-lysine content is at least 99% in chemically pure form.
Přednostou vynálezu sú nízké náklady na zariadenie, prevádzku a energiu. Chemický čistý L-lyzín možno z roztoku získat v tuhej formě sušením na valcovej alebo rozprašovačej sušičke alebo lyofilizáciou.The advantage of the invention is the low cost of equipment, operation and energy. Chemical pure L-lysine can be obtained from the solution in solid form by drying on a roller or spray dryer or by lyophilization.
Příklad 1Example 1
Technicky L-lyzín s obsahom L-lyzinu v množstve 86 % objemových vzhladom na obsah aminokyselin podlá analýzy automatickým analyzátorom aminokyselin a 68 % objemových obsahom L-lyzínu vzhladom na hmotnost sušiny sa odsolí gélovou filtráciou a rozpust! v destilovanej vodě v množstve 10 g.l . Získá sa tým roztok o vodivosti 800 ^us.cm- . Roztok sa autofokusuje pokial prúd neklesne pri 1 000 V na 1 mA a v aparatúre sa samočinné vytvoří gradient pH, kde pozdlž něho dochádza k separácii jednotlivých aminokyselin tvoriacich prlmes technického L-lyzínu. Samotný L-lyzín až v 96 %-nom zastúpení fokusuje vo frakciách blízkých pH 9,74. Roztok obsahujúci takto izolovaný L-lyzín sa znovu refokusuje a vo frakciách s pH 9,74 sa zhroraažduje chemicky čistý L-lyzín v koncentrácii aspoň 99,99 %. Nakoniec sa získaný L-lyzín lyofilizuje.Technically, L-lysine having an L-lysine content of 86% by volume based on amino acid content as analyzed by an automatic amino acid analyzer and 68% by volume of L-lysine content by dry weight is desalted by gel filtration and dissolved. in distilled water at 10 gl. Obtained in that a solution with a conductivity of 800 us.cm ^ -. The solution is autofocused until the current drops at 1000 V per 1 mA and a pH gradient is automatically generated in the apparatus, whereby the individual amino acids forming the bands of technical L-lysine are separated. L-lysine alone is up to 96% concentrated in fractions close to pH 9.74. The solution containing the thus isolated L-lysine is re-refocused and chemically pure L-lysine is collected in fractions of pH 9.74 at a concentration of at least 99.99%. Finally, the L-lysine obtained is lyophilized.
Příklad 2Example 2
Fermentovaná póda s obsahom L-lyzínu 20,0 g.l sa odstředí, upraví odsolením na iontomeničoch s výhodou na zeolite na hodnoty vodivosti 800^uS.cm a autofokusuje ako v příklade 1. Prvotná výťažnosi je 96 % oproti zvyčajnej výťažnosti 86 % a po refokusácii sa dosahuje chemický čistý L-lyzín v aspoň 99,99 % koncentrácii. Získaný L-lyzín sa suší v rozprašovače j sušičke.The fermented pod containing 20.0 g of L-lysine is centrifuged, desalted on ion exchangers, preferably zeolite, to conductivity values of 800 µS.cm and autofocused as in Example 1. The initial yield is 96% compared to the usual yield of 86% and after refocusing chemical pure L-lysine is achieved in at least 99.99% concentration. The L-lysine obtained is dried in a spray dryer.
Spósob výroby chemicky čistého L-lyzínu móže nájsť široké priemyselné uplatnenie v chemickom a farmaceutickom priemysle, potravinárstve, polnohospodárstve, výskume a diagnostike.The process of producing chemically pure L-lysine can find wide industrial application in the chemical and pharmaceutical industries, food, agriculture, research and diagnostics.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862922A CS265509B1 (en) | 1986-04-22 | 1986-04-22 | A method of producing chemically pure L-lysine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862922A CS265509B1 (en) | 1986-04-22 | 1986-04-22 | A method of producing chemically pure L-lysine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS292286A1 CS292286A1 (en) | 1989-02-10 |
| CS265509B1 true CS265509B1 (en) | 1989-10-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS862922A CS265509B1 (en) | 1986-04-22 | 1986-04-22 | A method of producing chemically pure L-lysine |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS265509B1 (en) |
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1986
- 1986-04-22 CS CS862922A patent/CS265509B1/en unknown
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| Publication number | Publication date |
|---|---|
| CS292286A1 (en) | 1989-02-10 |
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