CS264816B1 - Cultivating soil for the production of amylolytic enzymes - Google Patents

Abstract

The solution relates to the culture medium for the production of amylolytic enzymes by the production microorganisms. The culture medium according to the invention comprises 0.2 to 0.4% by weight sodium nitrate, 0.02 to 0.1% magnesium sulfate, 0.05 to 0.2% potassium hydrogen phosphate, 0.001% ferrous sulfate, 0.5 to 0.5% by weight. 10% of the old bread and the rest to 100% are water.

Description

(57) The solution concerns culture medium for the production of amylolytic enzymes by production micro-organisms. The culture medium according to the invention contains 0.2 to 0.4% by weight sodium nitrate, 0.02 to 0.1% magnesium sulfate, 0.05 to 0.2% potassium hydrogen phosphate, 0.001% ferrous sulfate, 0.5 to 10% old bread and the rest to 100% is water.

CS 264 816 Bl

The invention relates to a culture medium for the production of amylolytic enzymes by producing microorganisms.

To date, amylases have been produced by cultivating producers in nutrient media in which various types of starch are used as the carbon source. Potato starch, starch obtained from tubers of other plants, corn starch, crushed corn and cereal starch, or rice flour are most commonly used.

Ueda (UEDA, S. Trend Biochem. Sci., 6, 1981, p. 89) used raw potato starch, corn starch and cassava starch as a substrate for the production of amylases. Bhumibhamen (BHUMIBHAMEN, 0: Thai J. Agric. Sci., 16, 1983, p. 173) used rice starch and rice bran as a carbon source in the culture medium. Manomani et al. (MANONMANI, K., SHAMALA, T. R., SREEKANTIAH, K. R., J. Food Sci. Technol., 20, 1983, p. 38) used tapioca poppy and rice bran as a source of carbon in the culture medium. Dzilavjan et al. (JILAVJAN, L. R. et al .: Ferment. Spirt. Prem-st.,

5, 1985, p. 26) used corn flour and wheat bran. Wheat flour with corn steep liquor was also used by Tyulin et al. (TYULIN, V. S. et al .: Ferment. Spirt. Prem., 5, 1985, p. 29). Serichenko et al. (SERICENKO, L. G. et al .: Ferment. Spirt. Prem-st., 8, 1984, p. 33) tested starch, maltose, dextrins, and starch hydrolysates as a carbon source. In addition to the carbon source, the nutrient additives contain other substances that provide for the need for other macro- and microbiogenic elements needed for the growth of the production micro-organism.

Despite sophisticated amylolytic enzyme technologies with filamentous fungi, all methods have one common feature that can be considered a scarcity, since the carbon sources used in the nutrient media are otherwise useful, often economically more viable. In most cases, the sources of other nutrients used are a scarce maize leach (CSL), which is very needed, for example, for the production of antibiotics.

These disadvantages are substantially eliminated by the culture additive for the production of amylolithic enzymes by the production microorganisms according to the invention, characterized in that it contains 0.2 to 0.4% by weight of sodium nitrate, 0.02 to 0.1% by weight of potassium chloride, 02 to 0.1% magnesium sulfate, 0.05 to 0.2% potassium hydrogen phosphate, 0.01% ferrous sulfate, 0.5 to 10% old bread and the remainder to 100% is water.

The utilized carbon source in the culture according to the invention is old bread, returned from the retail network, or waste bread from your catering units such as casinos, dormitories, hospitals, and the like, which is still almost exclusively used for feeding to livestock.

Old, respectively. it is advisable to keep the waste bread dry to prevent mold. Molding up to 1/3 still allows the use of bread as a source of carbon, vitamins and growth factors in the culture medium.

Another benefit of the invention is the replacement of the organic nitrogen source from the corn liquor by a mineral source - using a defined culture supplement and further substituting growth stimulating substances added to the culture supplement by substances naturally present in the bread.

He did

A culture addition of 0.3% sodium nitrate, 0.05% potassium chloride, 0.05% magnesium sulfate, 0.12% potassium hydrogen phosphate, 0.001% ferrous sulfate, 2% old bread and the remainder water is prepared.

Aspergillus niger IV-123 CCM F 801, Aspergillus oryzal CCM F 8005 and the USSR strain of Aspergillus battatal were cultured under normal culture conditions (temperature 30 ° C, pA 7.0, aeration 50% volume of medium under min).

A very good production of amylolytic enzymes was found by comparing the culture additive according to the invention and the culture additive used hitherto (with corn meal as carbon source and calcium chloride as growth stimulator).

For example, in the production microorganism Aspergillus niger IV IV-123 <XW P 801 grown in the present invention, the alpha-amylase activity is about 89.10 n.kat.ml ^-1 and the total amylolytic activity is 352 n.kat.ml. ^-1 , production microorganism Aspergillus battatal (license strain USSRR alpha-amylase 105 n.kat.ml. ^-1 , glucoamylase 26 n.kat.ml. ^-1 and for other production microorganism Aspergillus oryzal CCM 8005 even alpha-amylase 1400 n.kat.ml. ¹ and glucoanylase 455 n.kat.ml ^-1 .

The use of the culture medium according to the invention increases the production of alpha-amylases and at the same time utilizes old waste bread to produce high-grade products suitable also for the food industry.

Claims (1)

OBJECT OF THE INVENTION Culture platform for the production of amylolytic enzymes by producing microorganisms, characterized in that it contains 0.2 to 0.4% by weight sodium nitrate, 0.02 to 0.1% potassium chloride, 0.02 to 0.1% magnesium sulfate, 05 to 0.2% potassium hydrogen phosphate, 0.001% ferrous sulfate, 0.5 to 10% old bread and the remainder to 100% is water.