CS262754B1 - Micromanipulator for embryo bisection - Google Patents
Micromanipulator for embryo bisection Download PDFInfo
- Publication number
- CS262754B1 CS262754B1 CS868642A CS864286A CS262754B1 CS 262754 B1 CS262754 B1 CS 262754B1 CS 868642 A CS868642 A CS 868642A CS 864286 A CS864286 A CS 864286A CS 262754 B1 CS262754 B1 CS 262754B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- embryo
- bisection
- micromanipulator
- microscope
- cutting tool
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/32—Surgical cutting instruments
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Surgery (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Wood Science & Technology (AREA)
- Transplantation (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Reproductive Health (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Microscoopes, Condenser (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Manipulator (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Predmetom vynálezu je mikromanipulátor na bisekciu embryí. Bisekcla embryí cicavcov je mikromanipulačná metoda umožňujúca rozdelenie embrya na dve polovice. Po následnej kultivácii a transfere týchto polovic do recipientiek slúži tento postup na získavanie identických dvojičiek. Bisekcia tak zváčšuje počet transferuschopných embryí, čo má význam predovšetkým pře zvýšenie úspěšnosti práce pracovitých skupin plemenářských podnikov transferujúcich embryá hovádzieho dobytka.The present invention provides a micromanipulator for embryo bisection. Bisekcla of mammalian embryos is a micromanipulation method allowing the embryo to be divided into two halves. After subsequent cultivation and transfer of these halves to recipients, this procedure serves to obtain identical twins. Bisection thus increases the number of transferable embryos, which is particularly important in order to increase the success of the working groups of breeding enterprises transferring embryos of bovine animals.
Doteraz známe postupy bisekcie používajú mikromanipulátory róznych konštrukcií riešené ako přístroje pře rožne mikromanipulačné zásahy, pričom bisekcia embryí je jednou z možností použitia. V podmienkach terénnej práce a z hTadiska potrieb plemenárskej praxe je v súčasnosti potřebná len bisekcia. Spoločným znakom týchto· mikromanipulátorov je, že zabezpečují! vedenie režného nástroja po embryu prostredníctvom mechanizmu zjemňujúceho pohýb lud- . skej ruky. Z toho vyplývá ich komplikovaná konštrukcia a vysoká cena.The prior art bisection procedures employ micromanipulators of different designs designed as devices to overcome micromanipulatory interventions, with embryo bisection being one of the applications. At present, only bisection is needed under the conditions of field work and from the point of view of the needs of breeding practice. The common feature of these micromanipulators is that they provide! guiding the cutting tool through the embryo through a human-softening mechanism. hand. This results in their complicated construction and high cost.
Uvedené nedostatky sú odstraněné mikromanipulátorom na bisekciu embryí podlá vynálezu, jeho podstata spočívá v tom, že jedno rameno nastavovacieho mechanizmu je připojené na objektiv mikroskopu a druhé rameno nastavovacieho mechanizmu nesie řezný nástroj, pričom s pohyblivými časťami mikroskopu tvoří funkčný celok.These drawbacks are overcome by an embryo bisection micromanipulator according to the invention, characterized in that one arm of the adjusting mechanism is attached to the microscope objective and the other arm of the adjusting mechanism supports the cutting tool, forming a functional unit with the moving parts of the microscope.
Mikromanipulátor na bisekciu embryí je zariadenie fungujúce na novom principe rezania embrya jeho vedením oproti řeznému nástrojů. Tento princip dovolil vývinů ť jednoduché zariadenie, ktoré po připojení na objektiv světelného mikroskopu umožňuje využit jemne ovládatelné pohyblivé časti mikroskopu a tak zabezpečit funkčnost’ tohoto spojenia.An embryo bisection micromanipulator is a device operating on a new principle of cutting an embryo by guiding it against a cutting tool. This principle allowed the development of a simple device which, when attached to a light microscope objective, allows the use of finely maneuverable moving parts of the microscope and thus ensures the functionality of this connection.
Příkladné převedeme mikromanipulátora je uvedené na priloženom výkrese, kde na obr. 1 je bokorys mikromanipulátora a na obr. 2 půdorys mikromanipulátora připojeného na objektiv 1 mikroskopu. Mikromanipulátor pozostáva z právej zvernej čelusti 2 a lávej zvernej čelusti 3, ktoré sú přepojené sťahovacou skrutkou 4. Sťahovacia matica 5 na sťahovacej skrutke 4 zabezpečuje přípoje,nie právej zvernej čelusti 2 a lávej zvernej čelusti 3 na objektiv 1 a prichytenie nastavovacieho mechanizmu 9 pravou zvernou čelusťou 2 a lávou zvernou čelusťou 3. Prídržná skrutka 7 prostredníctvom príložky 8 umožňuje fixovanie řezného nástroja 6 v požadovanej polohe. Přesné nastavenie režného nástroja 6 do, zorného pol'a mikroskopu zabezpečuje nastavovací mechanizmus 9 prostredníctvom nastavovacej skrutky 10.An example of a micromanipulator is shown in the attached drawing. 1 is a side view of a micromanipulator and FIG. 2 is a plan view of a micromanipulator attached to a microscope objective 1; The micro manipulator consists of a right clamping jaw 2 and a lava clamping jaw 3, which are connected by a tightening screw 4. The tightening nut 5 on the clamping screw 4 secures the connections, not the right clamping jaw 2 and the left clamping jaw 3 to the objective 1 and gripping the clamping mechanism 9 jaw 2 and lava clamping jaw 3. The retaining screw 7 by means of the shim 8 allows fixation of the cutting tool 6 in the desired position. Precise alignment of the cutting tool 6 into the field of view of the microscope is provided by the adjusting mechanism 9 by means of the adjusting screw 10.
Mikromanipulátor na bisekciu embryí sa upevní na objektiv 1 mikroskopu tak, aby nastavovací mechanizmus 9 bol orientovaný rovnoběžně s pozdlžnym pohybom stolíka mikroskopu. Príložkou 8 upevněný režný nástroj 6 smeruje špičkou do středu zorného póla. Jeho přesné nastavenie umožňuje pohyb mikromanipulátora okolo· osi objektivu 1, možnost rotácie horného ramena nastavovacieho mechanizmu 9 medzi pravou zvernou čelusťou 2 a 1'avou zvernou čeďusťou 3, posun nastavovacej skrutky 10 ako aj nastavenie polohy režného nástroja 6 pod príložkou 8. Špička řezného nástroja 6 sa používá jednak na rezanie, jednak na orientáciu embrya v kvapke média na podložnom sklíčku. Mikroskop je súčasne zaostřený na embryo i na špičku režného nástroja 6.The embryo bisection micromanipulator is mounted on the microscope objective 1 so that the adjusting mechanism 9 is oriented parallel to the longitudinal movement of the microscope stage. The cutting tool 6, which is fastened by the insert 8, points with the tip to the center of the visual pole. Its precise adjustment allows the micromanipulator to move about the axis of the lens 1, the possibility of rotating the upper arm of the adjusting mechanism 9 between the right clamping jaw 2 and the left clamping jaw 3, moving the adjusting screw 10 as well as adjusting the position of the cutting tool 6 below the shank. 6 is used both for cutting and for orientation of the embryo in a drop of medium on a slide. The microscope is simultaneously focused on the embryo and the tip of the cutting tool 6.
Posúvaním stolíka mikroskopu sa přiblíží embryo· ku špičke a pohybom embrya prostredníctvom stolíka mikroskopu ho 'špička gúla v kvapke média. Po dosiahnutí žiadanej polohy embrya sa řezný nástroj 6 prostredníctvom mikroskrutky mikroskopu jemne nadvihne a prostredníctvom pohybu stolíka sa embryo vsuinie pod řezný nástroj 6. Režný nástroj 6 sa pomocou mikroskrutky mikroskopu přitlačí na embryo. Potom sa pohybom stolíka mikroskopu posúva embryo smerom ku špičke režného nástroja 6 a rez je ukončený.Moving the stage of the microscope approaches the embryo to the tip and moves the embryo through the stage of the microscope to the tip of the ball in the drop of medium. After reaching the desired embryo position, the cutting tool 6 is gently lifted by the microscope microscope and the embryo is inserted under the cutting tool 6 by the movement of the stage. Then, by moving the stage of the microscope, the embryo moves towards the tip of the cutting tool 6 and the incision is complete.
Mikromanipulátor na bisekciu embryí je primárné určený pře potřeby pracovných skupin plemenářských podnikov transferujúcich embryá hovádzieho dobytka. Okrem toho ho možno použit’ aj na experimentálnych pracoviskách na bisekciu včasných stádií cicavčích embryí iných druhov hospodářských a laboratořnych zvierat od stádia dvoch buniek až po stádium blastocysty.A micromanipulator for embryo bisection is primarily intended for the needs of working groups of breeding enterprises transferring embryos of bovine animals. In addition, it can also be used in experimental workstations for the bisection of early stages of mammalian embryos from other livestock and laboratory animals from the two-cell stage to the blastocyst stage.
Claims (1)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CS868642A CS262754B1 (en) | 1986-11-26 | 1986-11-26 | Micromanipulator for embryo bisection |
DE19873738834 DE3738834A1 (en) | 1986-11-26 | 1987-11-16 | MICROMANIPULATOR FOR THE BISEECTION OF EMBRYONES AND METHOD FOR PERFORMING THE BISEECTION |
GB08727141A GB2198453A (en) | 1986-11-26 | 1987-11-19 | Micromanipulator for embryo bisection |
FR8716112A FR2607267B3 (en) | 1986-11-26 | 1987-11-20 | MICROMANIPULATOR FOR BISSECTION OF EMBRYOS |
PL26899487A PL268994A1 (en) | 1986-11-26 | 1987-11-24 | Micromanipulator for embryon bisection |
JP62295394A JPS63212349A (en) | 1986-11-26 | 1987-11-25 | Germ dividing micromanipulator |
HU532087A HUT46580A (en) | 1986-11-26 | 1987-11-26 | Micromanipulator for separating first the twins of mammals |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CS868642A CS262754B1 (en) | 1986-11-26 | 1986-11-26 | Micromanipulator for embryo bisection |
Publications (2)
Publication Number | Publication Date |
---|---|
CS864286A1 CS864286A1 (en) | 1988-08-16 |
CS262754B1 true CS262754B1 (en) | 1989-04-14 |
Family
ID=5437254
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CS868642A CS262754B1 (en) | 1986-11-26 | 1986-11-26 | Micromanipulator for embryo bisection |
Country Status (7)
Country | Link |
---|---|
JP (1) | JPS63212349A (en) |
CS (1) | CS262754B1 (en) |
DE (1) | DE3738834A1 (en) |
FR (1) | FR2607267B3 (en) |
GB (1) | GB2198453A (en) |
HU (1) | HUT46580A (en) |
PL (1) | PL268994A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3936571A1 (en) * | 1989-03-03 | 1990-09-06 | Zirm Mathias | Eye holding device for operating theatre - consists of cutting-block lined with elastic membrane that can be blown up with blow-support |
JPH05149840A (en) * | 1991-11-29 | 1993-06-15 | Shimadzu Corp | Micromanipulator |
-
1986
- 1986-11-26 CS CS868642A patent/CS262754B1/en unknown
-
1987
- 1987-11-16 DE DE19873738834 patent/DE3738834A1/en not_active Withdrawn
- 1987-11-19 GB GB08727141A patent/GB2198453A/en not_active Withdrawn
- 1987-11-20 FR FR8716112A patent/FR2607267B3/en not_active Expired
- 1987-11-24 PL PL26899487A patent/PL268994A1/en unknown
- 1987-11-25 JP JP62295394A patent/JPS63212349A/en active Pending
- 1987-11-26 HU HU532087A patent/HUT46580A/en unknown
Also Published As
Publication number | Publication date |
---|---|
HUT46580A (en) | 1988-11-28 |
JPS63212349A (en) | 1988-09-05 |
FR2607267B3 (en) | 1989-06-16 |
GB8727141D0 (en) | 1987-12-23 |
PL268994A1 (en) | 1988-09-01 |
GB2198453A (en) | 1988-06-15 |
DE3738834A1 (en) | 1988-06-09 |
CS864286A1 (en) | 1988-08-16 |
FR2607267A1 (en) | 1988-05-27 |
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