CS252455B2 - Method of streptolysin's o antibodies continuous determination in whole blood sample - Google Patents
Method of streptolysin's o antibodies continuous determination in whole blood sample Download PDFInfo
- Publication number
- CS252455B2 CS252455B2 CS815164A CS516481A CS252455B2 CS 252455 B2 CS252455 B2 CS 252455B2 CS 815164 A CS815164 A CS 815164A CS 516481 A CS516481 A CS 516481A CS 252455 B2 CS252455 B2 CS 252455B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- antibodies
- oxidized
- streptolysin
- blood sample
- reducing agent
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 30
- 210000004369 blood Anatomy 0.000 title claims abstract description 24
- 239000008280 blood Substances 0.000 title claims abstract description 24
- 108010011834 Streptolysins Proteins 0.000 title claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 206010018910 Haemolysis Diseases 0.000 claims abstract description 9
- 230000008588 hemolysis Effects 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims description 15
- 210000003743 erythrocyte Anatomy 0.000 claims description 14
- 238000002835 absorbance Methods 0.000 claims description 12
- 239000003638 chemical reducing agent Substances 0.000 claims description 11
- 239000011541 reaction mixture Substances 0.000 claims description 7
- 108010075210 streptolysin O Proteins 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 5
- 239000000644 isotonic solution Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 230000009089 cytolysis Effects 0.000 claims description 3
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 claims 1
- 230000002949 hemolytic effect Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 28
- 210000002966 serum Anatomy 0.000 description 14
- 239000000243 solution Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 4
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- VHJLVAABSRFDPM-UHFFFAOYSA-N 1,4-dithiothreitol Chemical compound SCC(O)C(O)CS VHJLVAABSRFDPM-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical group S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- -1 lyophilisate Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 125000004149 thio group Chemical group *S* 0.000 description 1
- 150000007944 thiolates Chemical class 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56944—Streptococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT23215/80A IT1132167B (it) | 1980-07-03 | 1980-07-03 | Metodo emolitico per la determinazione in continuo degli anticorpi antistreptolisina o,in campioni di sangue o di siero con impiego di streptolisina o ossidata |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS252455B2 true CS252455B2 (en) | 1987-09-17 |
Family
ID=11204965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS815164A CS252455B2 (en) | 1980-07-03 | 1981-07-03 | Method of streptolysin's o antibodies continuous determination in whole blood sample |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4379850A (it) |
| EP (1) | EP0043608B1 (it) |
| JP (1) | JPS5794659A (it) |
| AT (1) | ATE15546T1 (it) |
| CA (1) | CA1162846A (it) |
| CS (1) | CS252455B2 (it) |
| DE (1) | DE3172219D1 (it) |
| IT (1) | IT1132167B (it) |
| SU (1) | SU1367838A3 (it) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1218273B (it) * | 1981-05-07 | 1990-04-12 | Sclavo Inst Sieroterapeut | Metodo integrato per l'analisi degli anticorpi antistreptolisinici e mezzi adatti allo scopo |
| DE3210080A1 (de) * | 1982-03-19 | 1983-09-22 | Behringwerke Ag, 3550 Marburg | Anti-streptolysin o-latex-reagenz und verfahren zu seiner herstellung |
| US4554248A (en) * | 1982-11-08 | 1985-11-19 | American Home Products Corporation | Composition and method for detecting Antistreptolysin O |
| ES2037045T3 (es) * | 1986-09-24 | 1993-06-16 | Wako Pure Chemical Industries, Ltd. | Metodo nuevo para la determinacion del valor de antistreptolisina y un estuche utilizado para ello. |
| US5354846A (en) * | 1988-11-18 | 1994-10-11 | Michael Kehoe | Streptolysin O antigen derivatives, its production and uses |
| WO1999036778A1 (en) | 1998-01-20 | 1999-07-22 | University Of Victoria Innovation & Development Corporation | Detection of gpi anchored proteins |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1914081A1 (de) * | 1969-03-20 | 1970-10-01 | Behringwerke Ag | Verfahren zur Herstellung eines Antistreptolysin-Reagenzes |
| TR19262A (tr) * | 1975-12-22 | 1978-09-19 | Sclavo Inst Sieroterapeut | Antistreptolisin antikor'larin titrasyonu icin cihaz ve bu cihazin unsurlari vasitasiyle yapilan titrasyon usulu |
| FR2435020A1 (fr) * | 1978-08-29 | 1980-03-28 | Suovaniemi Finnpipette | Procede pour la mesure automatique semi-quantitative de l'intensite de couleur ou de la turbidite d'une solution liquide |
-
1980
- 1980-07-03 IT IT23215/80A patent/IT1132167B/it active
-
1981
- 1981-06-12 DE DE8181200658T patent/DE3172219D1/de not_active Expired
- 1981-06-12 EP EP81200658A patent/EP0043608B1/en not_active Expired
- 1981-06-12 AT AT81200658T patent/ATE15546T1/de not_active IP Right Cessation
- 1981-06-22 US US06/276,442 patent/US4379850A/en not_active Expired - Fee Related
- 1981-06-29 JP JP56099785A patent/JPS5794659A/ja active Pending
- 1981-06-30 CA CA000380895A patent/CA1162846A/en not_active Expired
- 1981-07-01 SU SU813304334A patent/SU1367838A3/ru active
- 1981-07-03 CS CS815164A patent/CS252455B2/cs unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US4379850A (en) | 1983-04-12 |
| SU1367838A3 (ru) | 1988-01-15 |
| ATE15546T1 (de) | 1985-09-15 |
| IT8023215A0 (it) | 1980-07-03 |
| JPS5794659A (en) | 1982-06-12 |
| EP0043608A1 (en) | 1982-01-13 |
| DE3172219D1 (en) | 1985-10-17 |
| EP0043608B1 (en) | 1985-09-11 |
| IT1132167B (it) | 1986-06-25 |
| CA1162846A (en) | 1984-02-28 |
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