CS243785B1 - Strain microorganism acromonium chrysogenum - Google Patents

Abstract

The invention relates to a microorganism strain Acremonium chrysogenum CCM F-750 producing cephalosporin C (hereinafter) CFS-C). This strain was obtained from the Acremonium strain chrysogenum CCM F-729 focused to increase celafosporin production C.

Description

(54) Strain of Acremonium chrysogenum CCM F-750

The present invention relates to a strain of cephalosporin C producing Acremonium chrysogenum CCM F-750 (hereinafter CFS-CJ.) This strain was obtained from a strain of Acremonium chrysogenum CCM F-729 by breeding aimed at increasing celafosporin C production.

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The present invention relates to a cephalosporin C producing Acremonium chrysogenum CCM F-750 strain (hereinafter CFS-Cj). This strain was obtained from the Acremonium chrysogenum CCM F-729 strain by breeding aimed at increasing cephalosporin C production. / ml, of which only about 1/3 formed the desired CFS-C, the remainder was desacetylcephalosporin C (D-CFS-C) and cephalosporin C (CFS-C _x j).

The described strain of the microorganism Acremonium chrysogenum CCM F-750 was obtained by subjecting the parent strain CCM F-729 to mutagenic sizing - alternately to UV light and N-nitrosomethylbiuret, preferring isolates resistant to feed back control of antibiotic synthesis-inhibition by CFS-C end product.

Breeding was performed by shifting the culture of the parental strain on the sloping agar in an End-tube on a sporulation tray NB-II, washing with 10 ml of sterile water, filtering through a cotton plug on a pipette to separate the hyphae and arthospores from conidia. Petri dishes having the composition NB-II having this composition were seeded from such a conidia suspension suitable for dilution. Bacto Beef extract 0.3%, bacto peptone 0.5%, glucose 1%, FeSO 4. 0.005%, MgSO4.

. 7H2O 0.005%, D, L-methionine 0.005%, L-cysteine 0.005%, Difco agar 2.5%.

Mutagenic interference was made using a UV light with a Phillips TUV 30 W germicidal UV lamp with a spectral energy distribution of a wavelength of 253.7 nm to 93.5%, indeed a wavelength of 6.5%. The distance between the dish and the radiation source was 30 cm. After irradiation, the plates were immediately transferred to an incubator at 28 ° C where they sporulated for 11 days. The developed colonies were isolated under aseptic conditions and transferred to sloping agar of NB-II.

Another mutagenic intervention was chemically done so that the conc. -10® 10 ⁵ conidia / / ml was added N-nitrozometylbiuret a concentration of 0.4%. The mutagen was in a culture cantact for 1 hour on a shaker (4 Hz) at 27 ° C. Following exposure was a mixture inutagéu-culture prepared by dilution of 10- ² to 10 ^_5 conidia / ml. The breeding was carried out in the absence of daylight, sporulation on Petri dishes lasted at 27 ° C for 11 days. Individual colonies were aseptically transferred to NB-II sloping agar and assayed for production activity in a flask selection.

PSda IP-5 having the following composition: sucrose 1.5%, ammonium acetate 0.5%, methylolaetate 8.8%, corn steep liquor (100%) was used as propagation submerged. After 72 hours of cultivation on a 4 Hz rotary shaker at 27 ° C, a vegetatively inoculum was obtained. A 500 ml boiling flask filled with a 40 ml PP-O pod was seeded from the inoculum. The production plates were as follows: corn starch 5%, peanut flour 6%, sucrose 0.5%, glucose 1.25 percent, CaCo3 1.0%, D, L-methionine 0.2%, borax 0.0%, a mixture of rapeseed and soybean oil 2.5 percent, (NH < t) 2SCU 1.0%, Csl. (100%) 0.5%.

After the cultivation on the shaker on the PP-0, the bred strain was left statically at room temperature for 5-7 days under the influence of its own metabolites. The level of the main metabolite CFS-C was gradually adjusted to 25,000 µg / ml at this passage. After that time, a monocolonium sowing was performed on the NB-IJ Petri dishes and the most productive PP-O subject was selected for screening.

The morphology of the strain is as follows: The obtained strain CCM F-750 forms wrinkled, cream-colored colonies with a colony diameter of about 2-5 mm on a sporulating soil NB-II. According to MPA on composition. , meat yield 1%, peptone mixture 1%, NaCl 0.5%, agar 1.5% are colonies of 3 to 7 mm irregular shape, cream color.

Biochemically, the strain obtained is characterized in that, compared to other Acremonium chrysogenum strains, only 0.05-0.3 percent of the degree of aeration is required in submerged fermentation.

The production activity of the strain is 10,200 µg / ml in flasks and 22,400 µg / ml in CFG-C.

Claims (1)

SUBJECT INVENTION Acremonium chrysogenum strain CCM F-750 producing cephalosporin C.