CS239379B1 - Method of continuous spectrophotometric measurement - Google Patents

Abstract

The continuous method is intended for spectrophotometric measurements. The essence of the method is that through a continuous mixing and adding device, measuring or cleaning media are added to the cuvette using dispensers from the reservoirs and from the funnel, samples reacted outside the cuvette and further, using a semi-automatic pipette, substances affecting the material in the cuvette. The continuous method of spectrophotometric measurement can be used in spectrophotometric laboratories and test rooms.

Description

The invention relates to a method of continuous spectrophotometric measurement.

Up to now, when measuring on a spectrophotometric instrument, one sample cuvette should be removed from the instrument and cleaned to measure the next sample. With modern spectrophotometric instruments, additional devices are beginning to be added, which greatly extend the applicability of these devices. In this way, some devices allow full-scale automated analysis to be performed. These devices are used mainly in clinical and industrial lahorators. A disadvantage is that these devices do not allow for more detailed monitoring of the reactions, since the preparation of the reaction associated with the light-absorption reaction takes place outside the spectrophotometric cuvette and the already reacted sample is delivered to the cuvette. However, basic research requirements are directed to studying the detailed course of reactions associated with the change in light absorption. This means that it is often necessary to carry out reactions directly in the spectrophotometric swing. At the same time, the productivity and quality of measurement currently available for spectrophotometric measurements does not make good use of the possibilities offered by the current spectrophotometric technique.

The above-mentioned drawbacks are eliminated by the continuous method of spectrophotometric measurement of the invention, which is added to the cuvette by means of metering or cleaning media from the reservoirs and from the funnel of the sample reacted outside the cuvette and further by means of a semi-automatic pipette. substances affecting the material in the rock.

The invention of the continuous method of spectroscopic measurement allows measurement without opening the cell cuvette cover and removing the cuvette to be cleaned outside the apparatus. It allows dosing of measuring and cleaning media directly into the spectrophotometric cell placed in the cuvette holder in the instrument. Various substances or suspensions of substances or suspensions of substances can also be added to the medium in the rock by means of a continuously mixing and adding device during the measurement. the particles whose reactions in the rock should be monitored. It allows to monitor the initial reaction rates and their immediate changes after the addition of new substances to the reaction mixture, also using the memory installed in the programming part of modern spectrophotometers.

The continuous method of spectrophotometric measurement improves the quality (cleanliness and dosing controlled objectively display device) and increases the productivity of work on modern spectrophotometers.

A continuous method of spectrophotometric measurement is shown schematically in the attached drawing.

In the measurement, a spectrophotometer 1 is used, in which the mixing device and the addition device 2, to which the controllable voltage source 3, the sample funnel 4, the media dispenser 6 of the media 5 and the pump 7 for suction to the 8. Through the continuously mixing and adding device 2, measuring or cleaning media is added (dosed) to the spectrophotometric cuvette by means of dispensers 6. From the funnel 4 are added samples reacted outside the cuvette and using a semi-automatic pipette, the substances by which we want to influence the material prepared in the swing of the spectrophotometer. Upon completion of the measurement, the effluent and the scrubbing media are aspirated through the pump 7 to waste 8. Media dosing and cleaning quality can be checked on the display of the spectrophotometer, which also determines that the device is ready for further measurement in a very simple manner.

The invention of the continuous method of spectrophotometric measurement can be used in spectrophotometric lahorators and test laboratories.

Claims (1)

SUBJECT Method of continuous spectrophotometric measurement, characterized in that a measuring or purifying device is added to the cuvette by means of dispensers from the reservoirs via a continuous mixing and adding device. SUMMARY OF THE INVENTION The medium and sample funnel are reacted outside the cuvette and further using a semi-automatic pipette of the fabric to affect the material in the rock. 1 sheet of exclamation