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Application filed by Viktor P Gracev, Andris E Gutmanis, Michail A Zavalnyj, Andris Ch Zicmanis, Maris K Kljabins, Valentina D PopovafiledCriticalViktor P Gracev
Priority to CS862346ApriorityCriticalpatent/CS269774B1/en
Publication of CS234686A1publicationCriticalpatent/CS234686A1/en
Publication of CS269774B1publicationCriticalpatent/CS269774B1/en
Immobilizing And Processing Of Enzymes And Microorganisms
(AREA)
Abstract
The process involves prodn of gelatin gel treated with transverse crosslinked reagents comprising dialdehydes; extrusion of the prod. through a sieve with opening of size e.g. 200-250 microns, treatment of the obtd. particles with a 0.25-0.75% soln. of proteolytic enzyme of animal origin using vol. ratio of enzyme soln to gel of 0.75-1.25:1; secondary treatment with dialdehydes; and finally addn. of reducing agents. USE/ADVANTAGE - In microbiological for culturing surface-dependent animal cells. - The prepd. micro-carrier is obtd. in higher yield and has improved quality. - (5pp Dwg.No.0/0)
CS862346A1986-04-031986-04-03Producing of microorganisms for animal cells cultivation
CS269774B1
(en)