CS269774B1 - Producing of microorganisms for animal cells cultivation - Google Patents
Producing of microorganisms for animal cells cultivation Download PDFInfo
- Publication number
- CS269774B1 CS269774B1 CS862346A CS234686A CS269774B1 CS 269774 B1 CS269774 B1 CS 269774B1 CS 862346 A CS862346 A CS 862346A CS 234686 A CS234686 A CS 234686A CS 269774 B1 CS269774 B1 CS 269774B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- dialdehydes
- animal cells
- gel
- reducing agents
- microorganisms
- Prior art date
Links
- 210000004102 animal cell Anatomy 0.000 title abstract description 3
- 244000005700 microbiome Species 0.000 title 1
- 239000000499 gel Substances 0.000 claims abstract description 6
- 108010010803 Gelatin Proteins 0.000 claims abstract description 5
- 239000008273 gelatin Substances 0.000 claims abstract description 5
- 229920000159 gelatin Polymers 0.000 claims abstract description 5
- 235000019322 gelatine Nutrition 0.000 claims abstract description 5
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 5
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 4
- 102000035195 Peptidases Human genes 0.000 claims abstract description 3
- 108091005804 Peptidases Proteins 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims abstract 2
- 239000002245 particle Substances 0.000 claims abstract 2
- 239000007863 gel particle Substances 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 230000000087 stabilizing effect Effects 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 230000001419 dependent effect Effects 0.000 abstract description 2
- 230000002906 microbiologic effect Effects 0.000 abstract description 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000001125 extrusion Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The process involves prodn of gelatin gel treated with transverse crosslinked reagents comprising dialdehydes; extrusion of the prod. through a sieve with opening of size e.g. 200-250 microns, treatment of the obtd. particles with a 0.25-0.75% soln. of proteolytic enzyme of animal origin using vol. ratio of enzyme soln to gel of 0.75-1.25:1; secondary treatment with dialdehydes; and finally addn. of reducing agents. USE/ADVANTAGE - In microbiological for culturing surface-dependent animal cells. - The prepd. micro-carrier is obtd. in higher yield and has improved quality. - (5pp Dwg.No.0/0)
Description
(57) Řešení ae týká oblasti biologie a mikrobiologiokého průmyslu, zejména pěstování povrchově závislých Živočišných buněk. Za účelem zvýšení výtěžku a zlepšení kvality výsledného produktu se částice gelu zpraoovávají 0,25 až 0,75 %ním roztokem proteolytického enzymu živočišného původu při objemových vzájemných poměrech roztoku enzymu k želatinověrnu gelu 0,75 až 1,25 : 1» a před přidáním redukčních Činidel se provádí druhotné zpracování dialdehydy.(57) The solution ae concerns the field of biology and the microbiological industry, in particular the cultivation of surface-dependent animal cells. In order to increase the yield and improve the quality of the final product, the gel particles are treated with a 0.25-0.75% solution of animal proteolytic enzyme at a volume ratio of enzyme solution to gel gelatin gel of 0.75-1.25: 1 and prior to the addition of reducing agents. The agents are subjected to secondary treatment with dialdehydes.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862346A CS269774B1 (en) | 1986-04-03 | 1986-04-03 | Producing of microorganisms for animal cells cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS862346A CS269774B1 (en) | 1986-04-03 | 1986-04-03 | Producing of microorganisms for animal cells cultivation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS234686A1 CS234686A1 (en) | 1989-01-12 |
| CS269774B1 true CS269774B1 (en) | 1990-05-14 |
Family
ID=5360318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS862346A CS269774B1 (en) | 1986-04-03 | 1986-04-03 | Producing of microorganisms for animal cells cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS269774B1 (en) |
-
1986
- 1986-04-03 CS CS862346A patent/CS269774B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS234686A1 (en) | 1989-01-12 |
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