CS228038B1 - Production of tissue preparation - Google Patents

Abstract

For the therapy and regeneration of cells in live organisms by extraction from an animal or vegetable tissue, the tissue is exposed to ethanol for 3 to 100 days at a temperature of 10-100 DEG C, preferably 70-85 DEG C. The thus obtained extract is further concentrated to 5-50% of its original volume; the concentrate is precipitated by ether; the precipitate is washed with ether, dried and dissolved in a solvent. Prior to extraction by 40-98% ethanol, the tissue is dried and comminuted. The ethanol extraction can also be performed in a multistage process, the extracts from the individual stages being intermixed before being concentrated. The animal tissue used is bovine blood or a mixture of cow and bull blood which, prior to its extraction by 85-98% ethanol, is dried at a temperature of 30-100 DEG C in an air stream, herring spawn or sperm; and the vegetable tissue is St John's wort herb.

Description

The invention provides a method of manufacturing a tissue preparation, in particular for the treatment and regeneration of cells in living organisms. It is a substance effect on the terrain of the macroorganism, and it is a substance adequate to the given state of the organism.

Previously, coarse extracts from various tissues have been used for this purpose, causing undesirable effects in the body

- 2,228,038 reactions and side effects, poor dosing and leading to allergic organisms. The current treatment concept is mostly aggressive. It aims mainly to liquidate mligní pathological cell resources that are currently celosvětověTprezentovany for ^D ev ^IM cytostati-alkyl. ^J and ^the known cytostatic chemotherapeutic agents are neetoxičtější medical lung, in addition to anti-cancer effects severely damaging healthy tissue toxicity manifested on mucous membranes, is responsible for bone marrow depression, allopecia etc., they are also hazardous to their immunosuppressive effects and teratogenic.

These drawbacks are overcome by a method of producing a tissue preparation, in particular for the treatment and regeneration of cells in living organisms, which is based on a method of making a tissue preparation, especially for treating and regenerating eyelids in living organisms, wherein the tissue preparation is obtained by extraction from an animal or plant woven extractive. agents, characterized in that the tissue is treated with ethanol for 3 to 30 days at a temperature of from 10 to 100 ° C, preferably from 70 to 85 ° C. The extract obtained is preferably concentrated to 5 to 50% of the original volume, the concentrate is precipitated with ether, the precipitate is washed with ether, dried and the solid is dissolved. in a solvent. The tissue prior to extraction is physically, optionally

chemically, dried and crushed, then the tissue is treated for 40 to gh; _v .

98¾ ethanol. Ethanol extraction can be carried out multistage and the combined extracts are further treated. In some cases, the concentrate obtained is precipitated with distilled ether. As

- 5,228,038 tissue raw material is used in bovine blood or a mixture of cow's and dagger's blood. The blood is dried prior to extraction with 85 to 98% ethanol - at a temperature of 50 to 100 ° C with simultaneous air flow.

In order to obtain effective tissue preparation complexes, it is necessary to select different steps from the simplest extraction with ethanol, possibly at different concentrations, to precipitation of these complexes of substances with ether from concentrated ethanolic extracts, followed by washing, drying and dissolving the dry matter. The ethanolic extraction is carried out at various concentrations. In some preparations, it is preferable to treat the woven fabric with 40%, with others up to 98% ethanol. This is done in extrusion vessels at a temperature of 10 to 100 ° C. Some weaving is preferably performed for a short-term, 1 to 2 days, for others a long-term, a week to 30 days. The same charge of the starting material is extracted with 2% or more. The obtained 2- to 8-degree extraxies are always mixed. Duration of the individual extraction steps: 1st and 2nd extraction last 24 h * 9K, ie 2 days, 3rd and 4th extraction 4 days, 5th and 6th extraction 10 days, 7th and 8th extraction 14 days . For some extracts, this time of the first, eighth and tenth extractions is reduced to 8 to 24 hours. 1 extraxce.

Prior to the ethanol extraction, the starting tissue is processed by grinding, drying, and comminuting. It is used for this purpose of a disintegrator and a hot-air electronic drier, where the temperature is 30 to 100 ° C. The qualitative diversity of tissue preparations is given by: different starting material (tissues treated with an extraordinary agent), different stages of extraction and possible

228 038

Coagulation of ethanol extract with ether * by other procedures / earsBeed blood is the most widely used starting tissue for lysing various tissue preparations with different effects. It uses ее mixtures of bull's and cow's blood, obtained from fresh and e-beasts in slaughterhouses. The cattle are subjected to veterinary examination and the blood is dried in a hot-air oven at a temperature of 30 to 100 ° C spilled on a tray up to 2 cm. The blood is filled into an extractor and extracted with 85 to ethanol. The extraction takes place, as said, on several scales according to the focus of the preparation on a particular noaological unit. The individual extracts ae were combined and concentrated to 10 100 to 100µg. The starting material (woven) was 1: 5 to 1:10 at a ratio of the extractant of jeriae. By this method of production, we will have beneficial effects in malignancy, improvement of general condition, x-ray image, reduction of pain, improvement of sleep, improvement of sleep, appetite, sometimes stabilization of the disease, inhibition of tumor growth until its regress and growth arrest. Positive influence on postactinic syndrome, prolonged life in bearable condition (tissue preparations from bovine blood) · In obliterating diseases of peripheral arteries, trophic changes, especially gangrene in M.Buerger, MJiaynaud, obliterating endarteritis, widens peripheral arteries, it acts anti-inflammatory and heals the entire disease process, even severe gangrene and ischemia syndromes. / Combination of tissue preparations from blood and St. John's wort, Hypericum perororum.

- 5,228,038 gynecological inflammations and in - degenerative joint diseases alleviate the ball preparation of herring eggs and joint problems and pain, so that the immutable become more affective. In ulcer disease (ulcue vetriculi et duodeň), a tissue preparation from perch stomach has a very positive effect on pain, healing process and general condition. Tissue preparations are completely non-toxic - and do not exert deleterious effects even after prolonged application.

The invention and its effects are explained in more detail in the following examples:

Example 1

It used 1000 m of fresh beef blood - the same piece of bull and cow, which was spilled on a stainless steel tray in a layer, »

- approx. 2 cm, and dried in a hot air oven. t for - 4 days. The dried blood is a black-brown amorphous mass that has been comminuted in a ball mill to form a crystalline powder which forms the extractor charge (105 g). Ethanol (500 m) was added to the cartridge and the extraction was performed on a water bath in the extractor at 80-85 ° C for 6 days, a total of 4 extraction steps, each with 500 m of extraction agent (totaling 2000 m).

^V Combined 4 extracts (mixed) with vacuum. Concentrate to 100 nL on a glass evaporator, allow to settle for 24 hours and filter under vacuum through filter paper. Evaporate the filtrate in a ground-glass flask in a ground-glass flask. Olparek / red-yellow-yellow amooTiní hmooa / has about 85% dry matter. The yield is 9.8 g. Oiparek is dissolved in 180 mL of ethanol. This solution

- 6,228,038 serves as a stock solution for the preparation of a tissue preparation for use in primary chronic progressive pollycatihitis, PCP, which has beneficial effects: arresting process alrtivity / decreasing SE to normal, eliminating morning stiffness and resting booestia, or reducing swelling, on weight, improving the aneme. Because it is a nontoxic treatment method with a harmless tissue preparation, it is a big exaggeration to the current therapy.

Example 2

2000 m. Of fresh beef blood, equally bull and cow, were dried on stainless steel trays in an electric mountain + air oven at a temperature of 80-1 ° C for 5 days. Dry blood Same as in Example 1 was added to the extractor in a 200 g load. 95% ethanol was added in a quantity of 1000 m. A total of 8 extractions were performed: first and second 2 days, third. and a fourth extraction of 4 days, a fifth and sixth extraction of 10 days, a seventh and eighth extraction of 14 days. A total of 50 days of extraction. The extracts were always withdrawn, the extracts mixed (combined), and the combined extract was concentrated in vacuo on a glass evaporator. After the extract has been withdrawn, the same volume (1000 m) of ethanol per cartridge is withdrawn. A total of 8000 ml, which was concentrated to 100 m. To this amount of the residue, double the volume of ether and shake and mix thoroughly. Vzin.] Kta ttaz = Luna. The mixture is filtered with sintine, washed with ether, suctioned off, dried and dissolved in redistilled water. This is the basic solution for a tissue preparation that has shown remarkable results in some of the bowel cancers of the gastrointestinal tract and lung, especially in combination with other tissue preparations *

- 7 Example 228 038

To 160 ml of the ethanolic residue from Example 2 was added twice the amount of ether and the resulting precipitate was collected.

The remaining alcoholic solution is filtered, concentrated on a circulating evaporator and finally in a distillation apparatus to a thick extract which is further evaporated on a water bath until a residue is obtained as a reddish brown viscous mass which is dissolved in ethanol (13 g of residue in 500 [mu] l) . This ethanolic solution is the basic solution for a tissue preparation that affects blood vessels and their diseases.

Example 4

Rapidly processed frozen and unsalted roe and roe sequences are freshly dried in a vacuum oven at a temperature after washing; approx. 75 ° C 2 to 5 days · The dried mass is ground and deposited in the skin of ^J farms. A homogeneous sample is taken for histamine determination.

1000 g of dried eggs and herring fill were used and added to the extraxtor with 5000 ml of ethanol. At a temperature of 75 to 80 ° C, it is extracted and after 24 g. and / or the same amount is added (i.e., 1000 ml).

of pure ethanol (95%). This is repeated in 6 stages, a total of 6 days and 1 day. The combined extracts were evaporated on a circular evaporator and the residue was concentrated to 200 ml solution. It is precipitated with half to the same amount of ether and the precipitate formed is filtered and dissolved in redistilled water to a 10% solution. • Yield 1% dry matter, ie 10.0 g · 10% stock solution is used to prepare a tissue preparation that has a beneficial effect on chronic dege- 8 228 038 non-native osteoarthrosis, with no localization differences, that is on both gonarthros (knee joint), and coxarthros (hip joint), spondylarthros (spine joints) and other joints. This medicine has a great resonance because it is effective and completely nontoxic as opposed to many medicines applied to these diseases. In addition, it has beneficial effects on pain and general condition, so that patients are more mobile and able to work.

Example 5

Used 1500 g of dried drug St. John's wort. Herb H ^p erici perforaai to which 12.5 L of 50% ethanol was added. Extraction was continued for 30 days in a container with kamninové výpuutí prortchévéní temperature for completion of ^p Mcerace through ^g ase clear extract is separated and the remaining herba was washed with 50% ethanol solution until the dye. The extract is turned into a syrup-like coinistility on a glass evaporator and dried in a vacuum oven at 40-60 ° C to dryness. Drying takes about 5 days. The yield of the dry residue is about 10%, i.e. 150 g. The dried residue, crushed in a furnace, is digested at 20 to 22 ° C with 0.3% phenol water for about 2 weeks with frequent agitation (Aqua redestillata).

Prior to this, the dry residue is digested. 95% alcohol (the residue was covered about 5 cm above the solid surface) and then digested with ether. Both digeece after approx. 5 hours. The solution was poured into water. Olpsarek is again poured with phenol water / first:

approx. 700 ml and the second approx. 230) -300 m. The two solutions were combined and left to rest at normal temperature for 6 weeks after filling. After this. over time the solution is filtered on sinter 3 ·

228 038 This gives about 1000 ml of a tan solution for preparing a tissue preparation from St. John's wort, which has poles.

_: valent anti-inflammatory and overall effect. In particular, it is applied. chronic gynecological inflammations, especially in resistant adnexal chronic infiltrates (inflammatory tumors) and together with a blood tissue preparation (in combination) in vascular diseases and as a regenerative component in inflammatory and degenerative liver cell processes. Its great advantage besides the therapeutic effects is that it is completely non-toxic and has no side effects. On the contrary, it positively affects the overall condition of the patients.

The method of manufacturing a tissue preparation, especially for the treatment and regeneration of cells in living organisms, is useful wherever degenerative and inflammatory processes occur. The advantage of said method is that they are obtained by selective extraction or further preparation of complexes of physiologically active substances, completely non-toxic, without side effects, precisely obtainable, effective in low concentrations and aimed at action in particular diseases.

Claims (1)

Subject vyáletu. 228 038 Method for producing a tissue preparation of rose: UME or animal tissue extraction of these tissues ethane-ol, optionally extracting agents in ^ ^{^ 1} ^ ^and Uující in that it is applied to the tissue etattlem more stages in the range of 3-100 days at a temperature of 10 to 100 ° C, preferably from 70 to 85 ° C; It is concentrated to 5 to 50% of the original volume, precipitated with ether or with a clear solvent, the precipitate is filtered through with ether, dried and the dried precipitate is dissolved.