CS225664B1 - Method of the saccharose inversion - Google Patents

Description

The issue of inversion of aaccharosis is discussed in PV 8043-75 (Jarý, Bacílek, Marková, Mark). The question of the source of invertase is very important for the enzymatic method of inversion. It is generally obtained from suitable strains of the microorganism. Schicketanz (1962) describes the use of invertase contained in honey to prepare invert sugars suitable for bee feeding. According to Rinaud et al (1973), honey invertase is of animal origin because it has been shown to be identical to bee pharyngeal invertase.

The activity of a number of enzymes has been found in pollen, eg alpha-, beta- amylase, phosphorylase (Iwanami, 1960), hexokinase (Teizo Katsumata, Yoshihisa Togasawa, 1968), catalase, peroxidase, polyphenol oxidase, proteolytic enzymes and invertase (Nevryanskaya, Dorochov) (1970), proteases and cellulases (Simeon, Spir, Dimitrov, 1974).

In another work, Teizo Katsumata and Yoshihisa Togasawa (1968 b) dealt in more detail with invertase activity in the pollen of Pinus densiflora, Zea mays and Cucurbita maxima, which are not very attractive to bees. He found that there was invertase in these pollen, but had little activity.

According to the process of the present invention, a concentrated microbially stable invert sugar solution is prepared by invertase contained in the plant pollen so as to achieve the desired degree of inversion of aacharose and the desired dry matter of the finished product. Preferably, the aacharose solutions are inverted by the suspended pollen under a specified thermal regime with constant stirring. As an enzyme source, pollen obtained from bees by pylochytes is preferably used. Inversion is possible

225 664

225 664 also use pollen that does not collect bees if they have sufficient invertase activity. The source of the enzyme that is suspended in the sucrose solution can be removed from the finished product by filtration or other suitable separation methods, but with respect to complete health safety it can be left in the finished product for use.

Example 1. 10 kg of sucrose is dissolved in 4.45 kg of water and a suspension of 10 g of pollen in 50 ml of water is added to the mixture, pH adjusted to 5 with water. The mixture is maintained at an optimal temperature for enzyme activity, typically 40 ° C. with continuous stirring up to 70% sucrose is inverted and the water content drops below 25% ·

Example 2. A suspension of 0.6 kg of pollen in 3 L of water is added to 1 ton of a 60 # sucrose solution (sucrose semi-finished product from a sugar refinery), the pH is adjusted to optimal for enzyme and suspension activity and maintained under stirring at 40 ° C to 70% of the sucrose present is inverted and the dry matter content increases to the desired limit.

Example 3. A suspension of 6 g pollen in 30 ml water adjusted to optimal for enzyme activity is added to 10 kg of 40 # sucrose solution, and the mixture is maintained at 40 ° C until the desired degree of inversion is achieved (up to 95% inversion can be achieved ). The suspended pollen is filtered off in pressure filters or separated on centrifuges. The mixture is concentrated at 60 ° C to the desired degree of sucrose. The water content can also be reduced on a vacuum evaporator.

Claims (2)

A method for inverting sucrose, characterized in that a solution or suspension of sucrose in water is hydrolyzed by invertase contained in pollen at pH = 4-8, preferably at pH = 6 and at a temperature of 22 to 55 ° C, preferably at a temperature of 40 ° C with stirring to the desired degree of inversion and dry matter of the product. 2. A method for inverting sucrose, characterized in that the pollen used can be separated from the finished product by suitable separation methods, preferably by filtration, flotation, or centrifugation.