CS211111B1 - A preparation for stabilizing water and protein homeostasis in calves or pigs and its production methods - Google Patents

Abstract

The preparation according to the invention will be included in the range of preventive and therapeutic preparations in the field of veterinary medicine. The indications of the preparation are: stabilization of water metabolism in diarrheal diseases, hypoproteinemia, dysproteinemia and immunodeficiency, hepatosis, intoxication, inflammatory processes with significant exudation, edema, acidosis and hemorrhagic diathesis, convalescence and low viability, stresses, etc. In the injectable preparation, the preparation contains per 1 liter 0.5 to 0.8 mmol of bovine or porcine albumin with an electrophoretic purity of 0.85 to 0.95 units, 68.4 to 102.6 mmol of sodium chloride, 4.0 to 6.0 mmol of potassium chloride, 111.0 to 166.5 mmol of glucose and 3.2 to 4.4 mmol of sodium caprylate. The declared purity of albumin is achieved by purifying crude albumin by denaturing unwanted proteins with heat in the presence of sodium caprylate without removing ammonium sulfate from the medium.

Description

1 211111

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition and a method of preparing an article for stabilizing aqueous and electrolyte metabolism in diarrhea, blood buffering and substitution and regulation of protein spectrum in hypoproteinemia and disproteinemia, especially in hypoalbuminemia in calves or pigs.

High incidence of diarrhic syndrome (64 95) and hepatoses (22%) (Slanina, L .: Industrial Cows Conservation and Its Concurrent Implementation, Veterinary, 29, 2, 1979) and High Occurrence of Hypoproteinemia (46 95), (Vajda , V .: Study of morbidity and resistance of calves in industrial conditions of breeding, Candidate dissertation thesis, 1979, VŠM Košice) requires pathogenetic, substitution intervention in teliat, especially when it comes to potentiating water homeostasis. Its restitution through the rehydrating agents alone is only a partial potentiation of water metabolism and electrolyte.

It does not solve or only imperfectly the loss of major nutrients (proteins, carbohydrates, fat i) and their associated negative balances, leading to the prevalence of catabolism over anabolism, manifesting itself in violation of the essential characteristics of living systems, i.e., growth arrest, loss of body weight, and so on. Also, the metabolic condition occurring in diarrhea-related calves in high-capacity tele-treatment due to incomplete therapy is serious. In this so-called postacidosis desiccation, tel'ata are often found to be "cured", resulting in not only a reduction in weight gain, but even a halt in calf growth. Therefore, in order to solve the disrupted aqueous and electrolyte metabolism, it is necessary to cope not only with the reduction of the volume of body fluids (dehydration), but also with the disorder of tonicity (hypotonia) and with the disorder of the acid-base condition (metabolic acidosis), (Oplištil, M .: Basics therapeutic administration of fluids to calves in large calf calves, Veterinary, 1, 978).

These disorders require both preventive and therapeutic measures to reduce the morbidity and loss of animals. The animal albumin, electrolyte, and glucose based formulation has proven to be appropriate and desirable for the aforementioned region, let us ask for a number of therapeutics or alternatives to be used. preventive measures, especially among young farm animals, one of the most important positions.

Disorders in the mineral metabolism of diarrhic syndrome are characterized by a decrease in sodium, potassium, calcium and magnesium levels in the blood serum. In severe dehydration associated with acidosis, potassium levels usually rise. Sodium and potassium from the qualitative hill represent the major blood serum electrolytes and are therefore almost a regular part of the rehydration therapy.

The rehydration therapy in veterinary medicine has been of major importance only after experience in human medicine. In the USSR, he developed a complex rehydration therapy for Sarabrinso with his school. In calves affected by dyspepsia, the first line of therapy is in i.p. papillins of treatment solutions in combination with dieto-, thermo-, pharmaco-, vitamin-, or haemotherapy.

Glucose is justified as a quick-to-use energy source due to the dietary constraints required in diarrhea therapy. Glucose is the safest source of parenterally delivered calories »It assimilates quickly, does not give rise to toxic products, is anticetogenic, saves protein, fat and water, is hepa -toprotective. It gives energy to cell metabolism. It is the basis of glycogen and a team of body tissues. It is known from experiments and clinical observations in the human field that getting glucose added to parenterally administered amino acids or proteins will ensure their optimal anabolization (Došková, M., Hainer, V., Rovenská, A .: Long-term Parenteral Nutrition, Time Physicians in the Czech Republic , 36, 1973). Glucose is also needed for salt and water resorption of 211111 2 from the small intestine in enterotoxemic diets (Rašková, H., Saehser, T., Vaneček, J.,

Treu, M., Mužík, J., Sklenář, V., Polák, L .: Peroral Rehydration of Diarrhea Diseases, Veterinary, 1, 1974). The effective binding of water and thus the electrolyte metabolism and the buffering of blood through albumin is promising in the current health situation in calves or pigs. However, in veterinary medicine, the use of animal albumin for the above indications is not yet known, while in human medicine, albumin has become very broad and effective.

Albumin is a highly effective stabilizer of aqueous homeostasis in the body. One molecule of albumin in the body of 18 molecules of water. It has a good buffering effect in the blood and adjusts the acid base. In hypoproteinemia and disproteinemia, it serves as a substitution and control agent for the protein spectrum, especially in hypoalbuminemia. Albumin in organisms is often catabolized mainly in gastroenteritis, pneumonia, pleuritis, enteritis, hepatitis, nephritis and others, so hypoalbuminemia is a frequent pathogenetic phenomenon with negative N-balance in the body and falls into the low viability syndrome especially in pups.

Considerable detoxification ability of albumin is suitable for endogenous and exogenous intoxications. In hepatoses, where albumin production is reduced, the albphic substitution is effective and promotes regeneration in the hepatic cell. Immuno-deficiency with concomitant hypoproteinemia may also be affected by albumin.

Albumin or albumin-like proteins are known in all animal species. The albumin molecule already exists in the first month of gestation. It guides virtually all metabolites, ions, amino acids, hormones, drugs, and toxic degradation products in organism.

Albumin is the most potent plasma protein in mammals. It is characterized by three distinct and closely related plasma functions. The first property - the size of the molecule, the charge and the concentration - contributes to the regulation of the colloid osmotic pressure in the plasma. Second, albumin, through its bonds and the spectrum of metabolites and metabolic effectors as well as through them, serves to transport the organism. The final albumin is produced as a distinct amino acid stock.

Albumin acts as the primary regulator of osmotic pressure, while plasma glo- bulins are less involved in its regulation. Keeping the molecular weight of the albumin is much smaller (69,000) than the molecular weight of the globulin (156,000), it contains a given amount of albumin of more osmotically active particles than the same amount of globulin; at a concentration of 1 g / 100 ml, the osmotic effect of albumin corresponds to 799.93 Pa and the osmotic effect of glo- bulin is only 199.98 Pa.

Because of their osmotic effect, plasma proteins tend to retain water in the blood capillaries and thus maintain plasma volume. In addition to the major metabolites, water-volume is the most prominent factor that affects plasma volume in cases of greater intestinal intake or loss from the body. In addition, water is constantly produced in all tissues as the final nutrient oxidation product. This movement of water within the body is much greater than the water exchange between the organism and the external environment. The maintenance of osmotic equilibrium results in the irreplaceability and importance of crystalloids and macromolecules - plasma proteins.

Cellular transport of albumin similar to its synthesis in the liver cell is similar to the formation and secretion of other proteins. An important role in the transport of albumin in the liver by the potassium cell in the liver cell. Albumin is fed not only to plasma and interstitial fluid, but is found in all body secretions and excretions. Approximately 40% of exchangeable albumin stocks are located outside the plasma space. 3 211111 \ t

I imagine skin and muscles! the largest stock of extravascular albumin. Quantitative albumin distribution may be partially altered by regulatory interventions of water and electrolytes that occupy space between organs as needed. In stressful situations such as burns, trauma, and infection, extravascular albumin rapidly replenishes plasma stocks, with extraplasmic reserves clearly changing. Recognizing the changes in extravascular albumin stores is therefore a prime indicator of the need for plasma.

Factors that directly affect albumin synthesis include: nutrition, hormone balance, environment, protein stock size and distribution, as well as some choices.

A decrease in serum albumin is a clinical concomitant symptom of many chorbias, while co-morbid syndrome is associated with a decrease in albumin production. According to the knowledge of human medicine, disease syndromes, which are found to have a regular decrease in albumin production, include: malnutrition, amino acid deficiency, (protein-calorie imbalania), malabsorption, cirrhosis, stress (surgical, early, burns) , infection), tumors, hypergammaglobulinaemia, hyperthyroidism, environment (acclimation to heat change), analbumemia, alcoholism, carbon tetrachloride and other liver toxins.

A special place in the range of liver diseases is occupied by its toxic damage to alcohol, especially to 1'home and organophosphates in animals, as well as other hepatodistrophic conditions, especially in dairy cows.

In all cases, hypoalbuminemia with frequent occurrence of plasmatic transfer and anomalous mucosa of the GIT occurs gradually, and the gastric content of albumin increases significantly, with the exception of gastritis (André, C., Descos, F., Descos, L., Lambert, R.,

Bory, R .: Gastric Clearence of Serum Albumin in Patients with Alcoholic Liver Cirrhosis, Digestion 14, 1976). In gastrointestinal diseases, the loss of plasma proteins can occur through a damaged mucous membrane or excessive lymphatic loss.

Although much is known about the synthesis and distribution of albumin, its degradation is only very clear. Isotopic studies have shown that the site of albumin degradation is in close proximity to plasma and extravascular space, but is in fact not a part of either. No organ has been reported to be at least partially responsible for the degradation of albumin. A small portion of the degraded amount of albumin per day is lost through the intestine, excreted by the kidney and metabolized by the liver.

Some other body fluids, such as bile, are involved in the process of secreting albumin into the body. In cases of renal impairment, elevated protein levels are excreted in the urine, or degraded in tubular meat, and similar in cases of elevated GIT pathway. Usually, in skin diseases, massive burns, or injury, esophageal albumin is swept or degraded in exudate areas. Human albumin has been used as a plasma substitute since 1942, when a group led by Evin. J. Cohnom developed · ·, methods of ethanol plasma fractionation. The method of ethanol fractionation was modified by several workers abroad and in the Czech Republic and also used the first infusions of albumin. In the further development of albumin isolation, neutral salts, metals, different immunoprecipitation methods were used.

The Hot Ethanol Technique (Schneider, W., Wolter, D., McCarty, L. J .: Technical Immunization in Heat-Ethanol Isolation of Serum Albumin. Blut 33, 1976) is less demanding and more economical than Cohn's ethanol fractionation. More recently, Sephadex, DEAE-cellulose, sepharose and various self-binding molecules of albumin have been used to isolate albumin, and in some cases up to 9856 yields are obtained (Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannel, R., Isolation of albumin from human plasma and fractionation of albumin plasma, Biochem J., 157, 1976).

Some properties of albumin in relation to its use in veterinary medicine: 211111 4 Feed proteins from the feed are digested in the upper tract tract by the fermentative system except for amino acids. These are resorbed in the small intestine and proteosynthesis occurs in its wall. This proteosynthesis has been shown to be carried out only by the formation of local fibrils which co-act in the resorption of amino acids. Own proteosynthesis of liver tract. A kind of protein pool is formed, which is baked to pass circulation and into tissues as needed.

It has been shown that there is no difference between this reserve protein and the blood plasma albumin. Plasma albumin can become a protein of tissues, and vice versa, protein tissue can be involved in the maintenance of blood plasma proteins, namely albumin. Also confirmed by trials with labeled albumin (Dorling, PR, Quinn, PS, Judah, JD: Evidence for Coupling of Biosynthesis and Secretion of Serum Albumin in the Rat. Biochem. Jí., 152, 1975), in which have shown that albumin is, under certain conditions, one of the most effective means of overcoming catabolic processes, especially in the GIT disease.

Administration of protein hydrolysates results in a significant increase in amino acid waste in urine and an increase in residual nitrogen. These factors indicate the metabolism of the transfected agent in the body. This process is not insignificant for the organism, as it consumes energy, and fermentative processes are depleted. This process is degrading on the one hand and resynthetic on the other is a heavy burden for the depleted organism, and thus it is an inadequate way of substitution from the metabolic point of view.

On the other hand, after transfusion! albumin builds up the entire molecule into those structures where hypoproteinemia has produced the greatest deficiency and where it causes the most serious defects of function. The success of therapeutic intervention is also in the rapid replacement of energetic protein.

Albumin is characterized by absolute utilization, non-antigenicity and harmlessness of the protein, which is inherent in the organism. After intravenous administration, it penetrates into the cells in three to ten minutes. On infusion of a small amount, 50% of the administered albumin leaves the bloodstream in the first day. This was confirmed by experiments with labeled albumin. The albumin from the bloodstream is directed to tissues where the need for protein is most urgent. It therefore has a nutritional and re-proteinant value.

The slight influence of albumin on total protein and nitrogen metabolism (elevation) can be explained by the fact that the administered albumin increases the volume of circulating blood by its colloid osmotic pressure, therefore its increased concentration cannot be proven.

One gram of albumin baths 15 to 17 ml (up to 23 ml) of fluid. This fluid flows mainly from the tissues into the bloodstream and dissolves proteins. Albumin levels are not increased by anineskflr, suggesting that albumin was used as whole protein. This view confirms blood and urine amino acid levels.

The increase in Na, K and Ca ions in the blood and urine after albumin occurs by increased aspiration of the intercellular fluid and increased filtration in the kidney channels.

The increase in circulating blood volume can be of varying degrees. It mainly depends on the blood reservoirs and the amount of mobilizable intracellular fluid. Smaller doses of al-bumin in the case of extensive edema cause a significant increase in circulating blood volume.

This fact is motivated by one of the anticipated indications of the product in the case of forward or post-natal edema in cows.

The albumin, electrolyte and glucose preparation is prepared as an isotonic solution with blood fluid (328 mOsm / L). In this composition, it assumes its use in diarrheal diseases of calves or pigs with various degrees of dehydration, 5 2111.1 in hypoproteinemia and disproteinemia, in immunodeficiencies, in conditions with increased albumin catabolism (exsudative conditions in the intestine, in the lining of the body, in renal disease, severe septicemia), hepatosis, intoxication of various etiology, recovery! and low viability of calves or pigs and in edema of the mammary gland.

The preparation according to the invention contains 0.5 to 0.8 mmol of bovine albumin, 68.4 to 102.6 mmol of sodium chloride, 4.0 to 6.0 mmol of potassium chloride, 111.0 to 166.5 mmol in 1 liter. glucose and 5.2 to 4.4 macl sodium caprylate. In this composition, the composition is intended for use in cattle, especially calves. Pig preparation - pig contains pig instead of albumin albumin.

The formulation is administered by injections intraperitoneally, intramuscularly, subcutaneously, rarely intravenously at a dose of 1 to 2 ml per kg bodyweight. Combination intraperitoneal and intramuscular or subcutaneous is suitable. Repeat for 24 resp. 48 hours is rarely needed, only for the most severe conditions. Tolerance is good. It uses sapreventive or therapeutical indications first. EXAMPLES Example 1 In a solution of the crude fraction of bovine or porcine albumin obtained after fractionation of bovine or porcine blood serum by salting out with ammonium sulfate, undesirable proteins are denatured at 58 ° C to 60 ° C and the actual acidity of the solution adjusted to pH 5 4 to 5.6 (2.7 mol / L hydrochloric acid) for one hour. Denaturation is carried out in a solution containing 25 to 35 g / l protein, 0.98 to 1.36 mol / l ammonium sulphate and 0.01 mol / l sodium caprylate. Heat denaturation of undesirable proteins in a sodium caprylate solution is carried out in a solution of the crude albumin fraction without treatment of the protein and ammonium sulfate, i.e. without removing the ammonium sulfate from the solution.

After cooling the solution to 40 ° C, the denatured, precipitated proteins are separated from the solution by centrifugation or filtration.

The purified diluted albumin solution is concentrated to a protein concentration of 70 to 90 g / l in solution by ultrafiltration, Centri-therm evaporation at a temperature of up to 50 ° C, or salting out of ammonium sulfate protein by 3.1 to 3.8 mol / l and redissolving the paste in distilled water to a concentration of 60 to 80 g / l of protein in solution. The albumin solution is dialyzed, gel-filtered, or ammonium sulfate ion exchangers to below 15 mmol / l and sodium caprylate below 4.5 mmol / l. In the albumin solution after clarification by filtration, the albumin concentration is adjusted to 0.5 to 0.8 mmol / l and the sodium chloride content is adjusted to 68.4 to 102.6 mmol / l, the sodium caprylate content to 3.2. 4.4 mmol / L of solution. The solution was further treated with potassium chloride for 4.0 to 6.0 mmol / l of solution and glucose for 111.0 to 166.5 mmol / l of solution. The raw materials used must comply with the requirements of CS3 3 for injectables. The current aciditar solution is adjusted to pH 7.2-7.4 with sodium hydroxide solution at 2.5 mol / l. The solution of the preparation is clarified by filtration and sterilized. The sterile injectable solution for injection is packaged in suitable containers.

The process used yields 12 to 15 g of protein from one liter of blood serum of bovine or porcine containing 85 to 95% albumin. The advantage of this process is that the purification of the albumin from the crude albumin fraction is carried out by denaturation without removing ammonium sulfate from the environment. EXAMPLE 2 From a solution of the crude fraction of bovine or porcine albumin obtained by) after fractionation of bovine or porcine blood serum by salting out with ammonium sulphate, the proteins are precipitated quantitatively with solid ammonium sulphate after being added to a final concentration of 2.6 to. 3.0 mol / l. The separated raw albumin paste by centrifugation or filtration by dissolution in distilled water to a solution having a concentration of 40 to 50 g per liter of protein in solution and 0.11 to 0.19 mol / l ammonium sulfate in solution.

Purification of the crude albumin fraction solution is accomplished by denaturing non-pure protein in this solution without removing ammonium sulfate at 58-60 ° C and adjusting the current acidity of the solution to a pH of 5.4-5.6 (2.7 mol / L hydrochloric acid solution). (j) for one hour. Denaturation of undesired proteins is carried out in a solution containing 0.02-0.03 mol / l caprylic acid.

After cooling the solution to 40 ° C, the denatured precipitated proteins are separated from the solution by centrifugation or filtration. In a further working procedure, concentration of the purified diluted albumin solution, actual acidity adjustment, clarification, sterilization, and filling is performed according to Example 1. The efficacy of the preparation is documented by the following assessment and tables. The preparation was used in calves of various ages and in various breeding technologies. The prevalence of the indication to verify the product was diarrhic syndrome, total hypotomy, cases of convalescence! after diarrhea or bronchopneumonia. The clinical effect after treatment with the preparation depended on the degree of the disease, the timeliness of the procedure, the resistance of the organism and the breeding level. The results of the product verification survey were evaluated both in terms of clinical effect in individual tested indications, but also in terms of laboratory findings of individual metabolic profiles. Repeated application of the product resp. in particular, the treatment required particularly severe cases of diarrhea with severe dehydration and appetite. Clinical improvement in health status occurred on the third - fifth day after treatment. The total losses due to death and necessary slaughter (from 3.9 to 5.5%) are lower than average for untreated or untreated. otherwise treated calves in the relevant period. Improved taste, resp. on the third day, in the case of treated calves, they should be evaluated positively for the purpose of the overall defense of the organism, since it allows, among other things, to accelerate the process by rehydrating the oral route.

Laboratory investigations and evaluations of metabolic profiles in a wide field of view have shown the adjustment of electrolytic and aqueous metabolism in calves, as well as the optimization of immunodeficient conditions, some enzymatic markers, the hepatic profile and the modification of disproteinemia. \ T

The preparation has been shown to be a highly effective therapeutic agent in diarrhea syndrome in calves, showing therapeutic efficacy on its own and moderately dehydrated, in a more severe stage it has a supportive effect in combination with rehydration agents or commonly used causal treatment . Very useful means of convalescence and low calf viability have been demonstrated, as well as in the prevention of low calf births and in the prevention of stress situations during the transfer to calves. In dairy cows, it had a very good therapeutic effect in cases of the occurrence of postnatal edema of the mammary gland, where it significantly reduced the progression of the disease compared to previously used agents. 7 2.1111

Table,

Therapeutic use of plasma solution in calves with diarrhic syndrome in the area of one line D I A R R H 0 E 'Opp. apl. Plasma- Ino Number Treatment Total Losses in% Object n 1'Easy Moderate Heavy Dilute Erythemid

Pdrod- nica 236 86, 08 42 24 48 223 9 4 5.5 ProfiLateroRio, 32 14 96 22, 2, 4, 25 6 ', 5.3 CT, 55 49 76 30 8, 1, 49 4 2 3,9 Summary 523 149, 280 94 44 73 497 19 7 4,97

Table Mineral Profile 2,. calves II. before (I.) and after (II.) treatment with plasma solution II. Ca I. P I. .11. Mg I. II. Fe I. II. Cu I. mmol / 1 yumol / 1 n 14, 14, 141 J39, 39 X 2.42 2.49 2.32 2.32 0.61 0.63 10.21 10.39 5.51 6.77 min . , 1, 92 1, 95 1.61 1.65 0.39 0.46 3.58 3.58 2.36 3.15 itiax. 2.96 2.99 3.1 3.33 0.83 0.87 18.27 21.13 8.66 10.23 + out of 8 4 2 0 0 1 0 0 0 0 X a 83 93 19 37, 0 11 0 0 0 0 -y 50 44 120 104 131 130 139 139 139 139 Γ + ζ 5.6 2.8 1.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 % | xa 58.9 66.0 13.5 26.2 7.1 7.8 0.0 0.0 0.0 0.0 Ly 35.5 31.2 85.1 73.8 92.9 92.9 100.0 100.0 100.0 100.0

Hepatic profile in calves before and after plasma solution Total bilirubin: GGT GOT GPT AP x 15.05 / 8.55 163/85 63/52, 6/44 16/13 iumol / l 0/1 o / i 0/1 0/1 increase in physiological cases 72 / 91.2 10.7 / 24.0 84 / 96.5 91.7 / 94.9 48.4 / 57 (+ 19.2%) (+ 13.3%) (+11) 211111 8

Table 4

The blood serum profile of calves before and after plasma solution treatment

Total protein: g / 1 x 51.3 / 54.8% increase in physiological cases 89/93 (+4.7 56)

Fibrinogen: g / 1 1.66 / 1.02

Albumin umol / 1,364.93 / 349.3 11/11 (+5 96) 57/70 (+13 96)

Table 5

Electrolyte profile in calves (in mmol / l) before (X.) and after (II.) Plasma solution treatment

Chlorides

TO

On I. II. I .. II. I. II. n 82 54 139 135 96 82 X 6.14 5.57 148.38 145.29 92.3 90.07 min. 4.35 4.50 108.75 122.24 70.07 76.98 max. 7.52 7.34 161.17 157.25 98.68 95.12

Table 6

Summary of the use of plasma solution in cattle

Category Number Age Diagnosis Number of hives Death + calves 39t 1 to 60 days Diarrhic syndrome 372 (95.14 96) 19 calves 523 1 to 60 days Diarrhic syndrome 497 (95.03 26 calves 10 8 to 13 weeks Respiratory syndrome 9 (90%) 1 calves 57 1 to 7 days Recuperation and low viability 53 (92,9 96) 4 dairy cows 5 3 years Milk edema 5 (100 96) - glands 9 211111

Table 7

Acidbase profile in calves before (I.) and after (II.) Plasma solution treatment pH p C02 BE BB SB Akt.BCOg log / molc to Pa mmol / 1 mmol / l. II. I. II. I. II. I. II. I. II. I. II. I. II. X 7.37 7.38 5.6 5.3 ->, o -1.3 47.3 46.4 23.6 23.3 24.2 23.4 25.5 24.6 min. 7,30 7,30 4,6 4,1 -4,7 -5,5 44,0 40,2 20,6 19,8 21,4 18,0 22,5 18,9 7,40 7 , 42 6.0 6 +4.0 +5.0 51, 0 55.0 '27, 8 28.8 26.5 31, 0 31, 0 32.5

Table 8

Differential blood count of leukocytes in calves before (I.) and soil (II.) Treatment with plasma solution

Eo Ba Ses Ty Ey Mo I. II. I. II. I.. II. I. II. I. II. I. II. n 140 140 140 140 140 140 140 140 140 (140 140 140 X 3 2.3 0.6 0.4 42 39 2.6 2.4 47 55 0,, 4 0, 3 min 1.8 ', 6 0.0 0.0 36 30 1.6 1.4 38 38 0,, 3 0,, 3 max 7.8 7.6 1.2 1.0 52 50 3.8 4.2 60 68 1 ,, 0 0. 8

Table 9 Basic haematological profile in calves before (I) and after (II.) Plasma solution treatment

Hb Er Le HKT I. II. I. II. I. II. I. II. g / dl T / 1 G / 1 1/1 n 140 140, 40 140 140 140 140, 40 X 9.3 9.8 5.588 5.748, 7.652 7.807 0.40 0.385 min. 6.65 6.81 3.748 4.054 4.254 4.868 0.30 0.270 Max 12.30 12.78 7.884 7.948 10.182 9.817 0.52 0.480 0 0 0 0 0 0 34.3 25.7% xa 52.8 67.1 44,3 61, 4 100, 00 42,9 51,4, -y 47,1 32,9 55,7 38,6 0,0 0 22,9 22,9

Claims (5)

OBJECT OF THE INVENTION A composition for stabilizing aqueous and protein homeostasis in calves or primates, characterized in that 1 liter of solution for injection contains 0.5 to 0.8 mmol of bovine or porcine albumin, 68.4 to 102.6 mmol of sodium chloride, 0 to 6.0 mmol of potassium chloride, 111.0 to 166.5 mmol of glucose and 3.2 to 4.4 mmol of sodium caprylate. 2. The composition of claim 1, wherein the bovine or porcine albumin is declared to have an electrophoretic purity of 0.85 to 0.95 units of bovine or porcine albumin. ♦ 3. A method according to claim 1, wherein bovine or porcine albumin is obtained from the crude fraction of albumin after fractionation of bovine or porcine blood serum by salting out with ammonium sulphate, separating the undesired proteins after denaturating them at 58-60 ° C and treatment. acidity of the solution to pH 5.4-5.6 in sodium caprylate and ammonium sulfate, followed by removal of ammonium sulfate, preferably by dialysis, after clarification the albumin solution is concentrated to a protein concentration of 0.5-0.8 mmol / l and adjusted the sodium chloride content is 68.4 to 102.6 mmol / l, the sodium caprylate content is 3.2 to 4.4 mmol / l, the solution dissolves 4.0 to 6.0 mmol / l potassium chloride, and glucose in an amount of 111.0 to 116.5 mmol / l and the solution obtained is made into a solution for injection. 4. A method according to claim 3, wherein the denaturing of the undesired proteins is carried out in a solution containing 25 to 35 g / l of protein, 0.01. mol / l sodium caprylate and 0.98 to 1.36 mol / l ammonium sulfate. 5. A process according to claim 3, wherein the denaturing of the undesired proteins is carried out in a solution containing 40 to 50 g / l protein, 0.02 to 0.03 mol / l caprylic acid and 0.11 to 0 , 19 mol / l ammonium sulfate.