CS204129B1 - Method of producing cellulolytic enzymes - Google Patents
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- CS204129B1 CS204129B1 CS13077A CS13077A CS204129B1 CS 204129 B1 CS204129 B1 CS 204129B1 CS 13077 A CS13077 A CS 13077A CS 13077 A CS13077 A CS 13077A CS 204129 B1 CS204129 B1 CS 204129B1
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- basidiotrichosporon
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- cellulolyticum
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- 108090000790 Enzymes Proteins 0.000 title claims description 11
- 102000004190 Enzymes Human genes 0.000 title claims description 11
- 230000001461 cytolytic effect Effects 0.000 title claims description 4
- 238000000034 method Methods 0.000 title description 5
- 229920002678 cellulose Polymers 0.000 claims description 8
- 239000001913 cellulose Substances 0.000 claims description 8
- 229920001503 Glucan Polymers 0.000 claims description 7
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 7
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 7
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 241001634922 Tausonia pullulans Species 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 239000001166 ammonium sulphate Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000000411 inducer Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 2
- 230000008020 evaporation Effects 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 1
- 239000011707 mineral Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- 229920001218 Pullulan Polymers 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 235000019423 pullulan Nutrition 0.000 description 4
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 3
- 229920002498 Beta-glucan Polymers 0.000 description 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 150000004676 glycans Polymers 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000002916 wood waste Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
(54) Spdsob produkcie celulolytických enzýmov(54) Method of producing cellulolytic enzymes
Vynález sa týká spósobu produkcie celulolytických enzýmov štepiacich celulózovú zložku lignin·—celulózových komplexov kvasinkovitými orgariizmami rodu Basidiotrichosporon.The invention relates to a method for producing cellulolytic enzymes that cleave the cellulosic component of lignin-cellulose complexes by yeast-like organisms of the genus Basidiotrichosporon.
Schopnost štepenia celulózy v prečistenom stave alebo v natívnom stave, vo formě lignin—-celulózového komplexu β (1 -» 4) glukán glukánohydrolázu EC. 3. 2. 1. 4, resp. schopnost produkovat tento enzým nie je vlastnosťou rodu Basidiotrichosporon ako celku. Pri použití gélovej metody testovania mikroorganizmov na ceiulázovú aktivitu (AO č. 183 169) sa zistila výrazná produkcia /3-glukán glukánohydrolázy u kmeňov zastupujúcich dva druhy rodu Basidiotrichosporon, a to B. pullulans a B. cellulolyticum.The ability to cleave cellulose in the purified state or in the native state, in the form of the lignin-cellulose complex β (1 → 4) glucan glucanohydrolase EC. 3. 2. 1. 4, resp. the ability to produce this enzyme is not a property of the genus Basidiotrichosporon as a whole. Using the gel method for testing microorganisms for cellulase activity (AO No. 183 169), significant production of β-glucan glucanohydrolase was found in strains representing two species of the genus Basidiotrichosporon, B. pullulans and B. cellulolyticum.
Žiadny z uvedených druhov nie je uvedený v literatúre ako producent enzýmov štepiacich celulózovú zložku lignin—celulózového komplexu.None of these species is reported in the literature as a producer of enzymes that cleave the cellulose component of the lignin-cellulose complex.
Podstata vynálezu spočívá v tom, že nasledovných 10 kmeňov rodu Basidiotrichosporon·.The principle of the invention is that the following 10 strains of the genus Basidiotrichosporon.
Basidiotrichosporon pullulans:Basidiotrichosporon pullulans:
CCY 30-1-1 a CCY 30-1-1 bCCY 30-1-1 and CCY 30-1-1 p
CCY 30-1-2 CCY 30-1-3CCY 30-1-3 CCY 30-1-3
Basidiotrichosporon cellulolyticum:Basidiotrichosporon cellulolyticum:
CCY 30-5-1 CCY 30-5-3 CCY 30-5-4 CCY 30-13-1 CCY 30-5-11 CCY 30-5-12 jednotlivo alebo ich zmesi kultiváciou na živnej pode minerálneho zloženia obsahujúcej síran amónny, gel hydroxyetylcelulózy a /3(1 -> 4) glukán ako induktor enzýmu pri teplote 20 až 40 °C, po dobu 48 až 110 hodin pri pH 4,5 až 6,8 produkuje komplexný celulázový systém /3-glukán glukánohydrolázu a /3-glukozidázu, ktorý sa získá v surovou stave odpařením rastovej pódy, připadne v čiastočne prečistenom stave zrážaním sířanom amonným, připadne zrážaním organickým rozpúšťadlom s výhodou etanolom.CCY 30-5-1 CCY 30-5-3 CCY 30-5-4 CCY 30-13-1 CCY 30-5-11 CCY 30-5-12 individually or mixtures thereof by cultivation on a nutrient medium containing ammonium sulphate, hydroxyethylcellulose α / 3 (1-> 4) glucan gel as enzyme inducer at 20 to 40 ° C, for 48 to 110 hours at pH 4.5 to 6.8 produces a complex cellulase system of β-glucan glucan hydrolase and / 3 Glucosidase, which is obtained in the raw state by evaporation of the growth medium, is optionally in a partially purified state by precipitation with ammonium sulphate or by precipitation with an organic solvent, preferably ethanol.
Všetky tieto kmene produkujú ureázu a ich biomasa dává s pufrovým roztokem diazofarbiva Fast Blue B červené zafarbenie, čo svědčí, že tieto kmene patria do oddelenia Basidiomycetes. Okrem typových kultúrAll these strains produce urease and their biomass gives a red color with the buffer solution of Fast Blue B diazo dye, indicating that these strains belong to the Basidiomycetes compartment. Excluding type cultures
204 1 2 9 (CCY 30-1-1, a, b, Btr. pullulans CCY 30-13-1 Btr. ínkin) bolí všetky kmene izolované z odpadových v8d drevospracujúceho a papierenského priemyslu. Kmene CCY 30-1-2 a CCY 30-1-3, CCY 30-5-11 a CCY 30-5-12 bolí izolované najnovšie v ČSSR.204 1 2 9 (CCY 30-1-1, a, b, Btr. Pullulans CCY 30-13-1 Btr. Inkin) hurt all strains isolated from waste wood and paper industry waste. Strains CCY 30-1-2 and CCY 30-1-3, CCY 30-5-11 and CCY 30-5-12 hurt isolated recently in Czechoslovakia.
Obidva druhy sa vzájomné odlišujú tým, že Btr. pullulans nevyužívá laktózu, trehalózu, ribózu ani xylózu, zatial' čo kmene Btr. cellulolyticum áno. Obidla druhy sa líšia produkciou xylanáz: Btt. pullulans xylanázy neprodukuje, zatial' čo Btr. cellulolyticum áno. Kmene druhu Btr. pullulans tvoria bohato artrospóry, kmene Btr. cellulolyticum len sporadicky.Both species differ from each other in that Btr. pullulans do not utilize lactose, trehalose, ribose or xylose, while strains of Btr. cellulolyticum yes. The grain species differ in the production of xylanases: Btt. pullulans do not produce xylanases while Btr. cellulolyticum yes. Strains of Btr. pullulans are rich in arthospores, strains of Btr. cellulolyticum only sporadically.
Btr. cellulolyticum sa od Btr. cutaneum (CCY 30-5-10- typová kultura a CCY 30-7-1 Btr. jirovecii — typová kultúraj líši aslmiláciou galaktózy, sacharózy, xylózy, strukturou polysacharidu, a prítomnosťou xylanáz a celuláz. Btr. cellulolyticum sa liší aj od iného susedného druhu Btr. inositophilum (CCY 30-5-5, -6 a -7], ktorého kmene sú schopné asimilovat inozitol.BTR. cellulolyticum is from Btr. cutaneum (CCY 30-5-10- type culture and CCY 30-7-1 Btr. jirovecii - type culture differs by the assimilation of galactose, sucrose, xylose, polysaccharide structure, and the presence of xylanases and cellulases. Btr. cellulolyticum also differs from another neighboring Inositophilum species (CCY 30-5-5, -6 and -7), whose strains are capable of assimilating inositol.
Použité kmene rodu Basidiotrichosporon sú uložené v Ceskoslovenskej zbierke kvasiniek a kvasinkovitých organizmov, Bratislava.The strains of the genus Basidiotrichosporon used are stored in the Czechoslovak Collection of Yeasts and Yeast Organisms, Bratislava.
Praktické využitie uvedených kmeňov spočívá v tom, že produkujú do kultivačného média enzýmy β-glukán glukánohydrolázu a /3-glukozidázu, ktoré sú jednoduchou metodou izolovatelné, pričom vznikajú z makromolekulárneho substrátu typu /í(l -* 4] glukánu oligoeacharidy typu celobiózy, ceilotriózy, celotetraózy a D-glukóza. Enzýmy uvedeného typu sú využitelné v procese sacharifikácie celulózy a v základnom výskume. Ďalšia možnost využitia tejto vlastnosti uvedených kmeňov rodu Basidiotrichosporon je v hydrolytickom rozklade dřeveného odpadu za vzniku enzýmatického hydrolyzátu priamo utilizovaného nahromaděním nutričně hodnotnej bielkoviny kvasinkovitých Basidiotrichosporon využitelných v krmovinárstve. Kvasinková forma Basidiotrichosporon umožňuje použitie ako centrifugačných tak aj filtračných separačných metód k oddeleniu nakultivovanej biomasy.The practical use of these strains consists in the production of β-glucan glucanohydrolase and β-glucosidase enzymes in the culture medium, which can be isolated by a simple method, resulting from a cell type macromolecular substrate of β (1 - * 4) glucan of the cellobiose type, ceilotriosis Enzymes of this type are useful in the process of saccharification of cellulose and in basic research Another possibility of utilizing this property of said strains of the genus Basidiotrichosporon is in the hydrolytic decomposition of wood waste to form an enzymatic hydrolyzate directly utilized by the accumulation of nutritionally valuable yeasts The yeast form of Basidiotrichosporon allows the use of both centrifugation and filtration separation methods to separate cultured biomass.
PřikladlEXAMPLE
Z gelu žo sieťovanej hydroxyetylcelulózy (A. O. č. 183 169) sa vyrežú trojúhelníkové profily o straně 4 cm, vložia sa do Petrlho misky a zalejú sa roztokom kultivačného média Yeast Nitrogen Base (Oxoid) s gélom hydroxyetylcelulózy ako jediným zdrojom uhlíka, s přísadou induktora (3(1 - 4) glukánu (1 mg/100 ml), tak aby pol hrůbky gélu vyčnievalo nad hladinu kultivačného média. Po trojnásobnej sterelizácii pri 100 °C po dobu 1 hodiny sa očkuje na povrch gélu kultúra Basidiotrichosporon pullulans CCY 30-1-2 a kultivuje sa pri 28 °C po dobu 48 hodin. Stekutený gél spolu s pomnoženou biomasou B. pullulans (0,42 gj sa použil ako inokulum na produkčnú pódu (2 1) s 1 °/o kukuřičného výluhu, 0,18 % hydroxyetylcelulózy, 0,2 % síranu amónneho a 0,2 % dihydrofosforečnan draselný (pH produkčněj pódy bolo upravené na 5;9). Kultivácia prebiehala po dobu 96 hodin pri 28 °C. Po ukončení kultivácie sa biomasa odcentrlfugovala (1500 g, 10 min.) a supernatant sa zahustil na odparke. Získal sa surový enzymový preparát s celulózovou aktivitou 66 U a specifickou aktivitou 0,78 U/mg. Příklad 2Triangular profiles of 4 cm side are cut from the gel of cross-linked hydroxyethylcellulose (AO No. 183 169), placed in a Petrl dish and embedded with a solution of Yeast Nitrogen Base (Oxoid) with hydroxyethylcellulose gel as the sole carbon source, with an inducer ( 3 (1 - 4) glucan (1 mg / 100 ml), so that half the depth of the gel protrudes above the culture medium After three-fold sterilization at 100 ° C for 1 hour, a culture of Basidiotrichosporon pullulans CCY 30-1- 2 and cultured at 28 [deg.] C. for 48 hours Liquid gel together with expanded B. pullulans biomass (0.42 gj was used as inoculum on a production platform (2 L) with 1% corn extract, 0.18% hydroxyethylcellulose, 0.2% ammonium sulfate and 0.2% potassium dihydrophosphate (pH of the production stage was adjusted to 5.9) The cultivation was carried out for 96 hours at 28 [deg.] C. After the cultivation, the biomass was centrifuged (1500 g, 10 min). . ) and the supernatant was concentrated in a evaporator to give a crude enzyme preparation with a cellulose activity of 66 U and a specific activity of 0.78 U / mg. Example 2
Rovnako, ako v příklade 1, s tým rozdielom, že namiesto BasldiiotriehoeporOn :piullulans sa použil kmeň Bassidiotrichosporon cellulolyticum CCY 40-5-4. Výsledný surový enzýmový preparát mal aktivitu 94 U a špecifickú aktivitu 0,71 U/mg.As in Example 1, except that the strain Basidiotrichosporon cellulolyticum CCY 40-5-4 was used instead of BasldiiotriehoeporOn: piullulans. The resulting crude enzyme preparation had an activity of 94 U and a specific activity of 0.71 U / mg.
P r í k 1 a d 3EXAMPLE 1 a d 3
Rovnako, ako uvedené v příklade 2, s tým rozdielom, že namiesto kukuřičného výluhu a hydroxyetylcelulózy sa ako zdroj uhlíka v kultivačnej póde použije odpadná vata z hydroxyetylcelulózy (50 g/1).As in Example 2, with the exception that corn leach and hydroxyethylcellulose instead of hydroxyethylcellulose waste cotton (50 g / l) is used as the carbon source in the culture medium.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS13077A CS204129B1 (en) | 1977-01-10 | 1977-01-10 | Method of producing cellulolytic enzymes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS13077A CS204129B1 (en) | 1977-01-10 | 1977-01-10 | Method of producing cellulolytic enzymes |
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| Publication Number | Publication Date |
|---|---|
| CS204129B1 true CS204129B1 (en) | 1981-03-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS13077A CS204129B1 (en) | 1977-01-10 | 1977-01-10 | Method of producing cellulolytic enzymes |
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| Country | Link |
|---|---|
| CS (1) | CS204129B1 (en) |
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1977
- 1977-01-10 CS CS13077A patent/CS204129B1/en unknown
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