CS201150B1 - Method of conservation and fortification of the green voluminous fodder - Google Patents

Description

Protein green fodder is preserved in agriculture, as is known, with lactic acid, which results from the addition (silage) of protein fodder by adding a certain amount of beet molasses, which is fermented to lactic acid by the action of Lactobacillus.

Beet molasses as a residual product of beet sugar production, however, is becoming a deficient material due to improved sugar technology by better utilization of its sucrose content and increasing demands of the fermenting industry, which needs molasses as a starting material product for differently important products (alcohol). , citric acid, lactic acid and the like). Even the partial import of cane molasses does not solve the persistent lack of beet molasses.

Detailed experiments have shown that it is possible to replace defective beet molasses with wood hydrolyzate to preserve and fortify green bulk forage.

Wood hydrolyzate (eg wood molasses obtained by concentration of waste water from the production of fiber boards by wet process, DVD m.sp ,, or obtained by concentration of wood hydrolyzates after exposure to hydrolyzing agents such as acids, hydroxides, etc., in the form of about 50% solution) a significant amount of carbohydrates that can replace canola molasses in canning.

The use of wood hydrolysates (wood molasses) for feeding purposes is the subject of a separate author's certificate no. No. 156,673. However, it is not known from the available literature for its use for the preservation and fortification of green bulk fodder, which is the object of the present invention.

The wood hydrolyzate contains solubles, mainly of a carbohydrate nature, in the form of mono- and oligosaccharides, which are formed from wood during thermomechanical pulping of chips by saturated steam or by action of hydrolyzing agents. Wooden molasses from DVD production

m.sp. has the following composition® (calculated on dry weight):

- ethereal extract (bituminous substances) 0,40%,

- ethanol extract (monosaoharides, etc.) 20,50%,

- lignin (determined with sulfuric acid) 9,0%,

- carbohydrate fraction 66.0%, of which monosaoharides 8.0%,

- ash (K _o 0, OaO, MgO, Pe _o 0_, SiO 2, Al ₉ 0, P _o 0_, MnO, traces of Cu,

4.00.%.

B, Ni, Co)

- Crude protein 0,10%,

Concentrated wood hydrolyzate in an amount of 0.5 to 10% by weight can be used for preservation and fortification. Examples of experiments using wood molasses in amounts of 0.5 and 1 wt. from preserved fodder.

Example 1-0.5%

Natural grass cover from the first mowing was used for conservation. The crop was harvested at the predominant grass stage (Pestuoa pratensis Huds., Poa pratensis I., Dactylis glomerata I,). The experiment was based on type forces with a capacity of 25 1. Each variant was carried out in five repetitions. The control variant represented grass without preservative.

The wood hydrolyzate was applied in an amount of 0.5% by weight. of conserved grass. Prior to application, it was diluted 1: 1. Beet molasses as a traditional preservative was used according to the same principles.

The course of the fermentation process was monitored according to CO ₂ production and temperature. Based on the analysis of grass matter for organic nutrients before and after the fermentation process, nutrient losses during the preservation were calculated. After the fermentation process was finished, the lawn grass was evaluated according to ČSN 46 7012.

The maximum temperatures achieved in the 28.2 and 28.8 ° C preservation variants indicate the correct course of the fermentation process. * In contrast, in the control variant, the maximum temperature of 39.6 ° C indicates that the temperature optimum for lactic acid bacteria has been exceeded and thereby favorable conditions for undesirable microflora have been created.

The increased oxidation activity during the fermentation process in the control variant also appeared in increased CO ₂ production over other variants.

The overall evaluation (Table 1) of the conserved grass mass shows that substantially identical results were obtained for wood hydrolyzate and urep molasses. Said preservatives ensure the correct course of the fermentation process, which is reflected in the same classification classification.

Table 1

Overall evaluation of conserved grass matter (0.5% of the additive)

variant n pH Content ganic acids δ mass] Color fragrance structure The quality the class lactic acid butter inspection 5 4.75 1.23 l, 9 g 0,230 brownish green she acidified butyric acid partially disrupted III Hydrolysate 0,5% 5 4.48 4.04 0.92 0,000 green fairly acidic maintained I Beet molasses 0.5% 5 4.44 4.83 0.70 0,004 green fairly acidic preserved I

Example 2 - 1%

The procedure was similar to that of Example 1, except that the wood hydrolyzate and beet molasses were used at a rate of 1% by weight. of conserved grass. Homofermentative lactic acid bacteria were used as the SalSi variant.

The fermentation process was monitored according to the same criteria as in Example 1.

The maximum temperatures reached (wood hydrolyzate 28.8 ° C, beet molasses 26.9 ° C, homofermentative lactic acid bacteria 27.1 ° C) signal the correct course of the fermentation process. In the control variant, a maximum temperature of 37.8 ° C indicates that the temperature optimum for lactic acid bacteria activity has been exceeded, thereby creating favorable conditions for the activity of Coli and Z total recovery (Table 2) of erogenes and butter fermentation. of conserved grass matter implies that Table 2

The overall evaluation of the end of the treated grass matter (1% of the additive)

variant n pH Content organio hmo acids] Color Vanya structure The quality category lactic octo Your butter inspection 5 4.69 1.47 2.0 3 0,170 brownish green acidic after butyric acid partially disrupted III Hydrolysate 1% 5 4.22 3.58 0.6 2 0,000 olivo- vozele- on the fairly acidic preserved I. Beet molasses 1% 5 4.32 4.23 0.6 0 0,000 olivo- vozele- on the fairly acidic maintained I Lactic acid bacteria 5 4.42 4.12 0.4 8 0,030 olivo- vozele- on the fairly acidic preserved I

yielded substantially identical results for wood hydrolyzate, beet molasses and homofermentative lactic acid bacteria. Said preservatives ensure the correct course of the fermentation process, which is reflected in the same classification classification.

The results obtained make it possible to conclude that beet molasses can be successfully replaced by a concentrated wood hydrolyzate in the conservation of grass matter.

Exactly obtained results confirmed that when preserving:

a / the temperature curve in the canned fodder reaches a maximum after 10 to 12 h and after 8 days the temperature is equal to the ambient temperature. A maximum temperature not exceeding the optimum temperature suitable for the activity of lactic acid bacteria b} / High C0 ₂ formation was recorded after 13 to 15 days. 20032004, 04:57:07 Over the next period, the level remains at the same level (c) in canned fodder prepared with beet and wood molasses, there has been no occurrence of butyric acid, or only a small amount (d) a high percentage of lactic acid is reached; (e) pH values achieve optimum nutrient losses, in particular on nitrogenous substances, at normal levels.

As a source of thickened wood hydrolyzate we mentioned waste water from DVD production

m.sp. Of course, molasses produced by neutralizing and concentrating solutions (hydrolysates) obtained by deliberate hydrolysis of wood may also be used.

By monitoring new possibilities for the preparation of agricultural feed from new sources while maintaining the quality of the feed and improving the environment, they concluded that preparation is possible - preserving and fortifying green bulk feed with thickened wood hydrolyzate (wood molasses), which allows full replacement of defective beet molasses . They pointed out the results achieved under strictly monitored conditions.

Preservation and fortification of green fodder by a new addition of concentrated wood hydrolyzate is characterized by the following advantages:

1. It ensures the use of wood molasses prepared from waste products from the production of DVD m.sp. or from wood hydrolyzate and represents a full-value substitute of the previously used beet molasses.

2. That resource is currently unwanted waste (waste water) or is a raw material (wood), which is a domestic raw material and, with proper management of forest resources, still renewable.

3. Provides both preservative and energy substances.

4. It does not require any investment costs as the same equipment is used as in canning with beet molasses.

5. Improves the environment by making waste water from the production of DVD m.sp. do not pollute public flows when discharging ·

Claims (1)

DISCLOSURE OF THE INVENTION A method of preserving and fortifying green bulky forage by adding sachetical products and by treating with a lactic acid "in situ", characterized in that, prior to covering the fodder, preferably even before squeezing, the fodder adds 0.5 to 2% by weight of the thickened wood hydrolyzate prepared by vacuum concentrating the waste water from the fiberboard production by wet spraying / or vacuum thickening of the wood hydrolyzate obtained by hydrolysis.