CS200795B1 - Biomass production method - Google Patents

Description

The present invention relates to the production of biomass by Torulopsis kruisii for feeding purposes.

The lack of protein feed, the need for fortification with vitamins and amino acids, as well as the problems of recovering bulk waste in agriculture and forestry, require the use of special yeast species for biomaay production.

The species used so far, Saccharomyces cerevisiae, S. carlsbergensis, Candida utilia, use mainly monoaaccharides - hexoses or di - or. oligosaccharides, composed of hexos. These yeasts are very sensitive to detailed types of inhibitors that occur in nontraditional wastes and their hydrolysers, as they are detailed colloidal colored substances of indeterminate composition.

Therefore, for hydrolysates and pre-hydrolysates from wood waste, sulphite leaches from paper and pulp, for hydrolysates from animal excrements from large-scale, poultry and other waste, other organisms need to be selected. They are mainly required to use d-xylose, 1-arabinose, glucuronic and galacturonic acid, resistance to volatile acids such as lactic and acetic, respectively. ability to utilize these acids.

Such properties have the species Torulopsis kruisii (Kock-Kratochvílová and Ondrušová (Biology 26: 477, 1971)), a yeast isolated from the surface of large inedible fungi such as Amanita muscaria, A. porphyria, Boletus satanas, Lactarius vellereus and others. The presence of toxic or bitter substances in large fungus crops does not interfere with this yeast. It can also cleave the beta-glucosidic linkages of polyeacherides produced by these fungi.

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SUMMARY OF THE INVENTION The present invention provides a method for producing biomass by culturing on a hydrolyzate from animal excrement or from wood-processing industrial waste, or a hydrolyzate from plant material, characterized in that the hydrolyzate is adjusted to pH 5 → 5 to 6.5 by addition of solid calcium hydroxide. % ammonium sulphate, hydrolyzate is filtered with activated carbon, seeded with Torulopsis kruieii strain CCX 26-19-7, or CCX 26-19-3, or OCX 26-19-4 or CCX 26-19-5, or CCX 26 -19-6, or CCX 26-19-8, or CCX 26-19-9, or CCX 26-19-10, or CCX 26-19-11, or CCX 26-19-12, or CCX 26-19 -13, or CCX 26-19-14, or CCX 26-19-15, and grown under ventilation at 25-30 ° C.

Strains of Torulopsis kruisii can be used to accumulate biomass with a relatively good crude protein content above 40%. The strains are easy to grow on hydrolysates from various non-traditional wastes and resist well present colloidal and color inhibitors. Hydrolysates or pre-hydrolysates of wood, animal excrements and similar wastes may be used without removal of the inhibitors or after their adsorption to pulverized coal.

The strains of the species Torulopsis kruisii are characterized by the fact that they form single cells oval with dimensions 2 to 4 x 3 to 5 pm, you do not form hyphae or pseudohyphae. Huge colonies on wort agar are white, soft, smooth. The cell surface / volume quotient is 1.5 to 2.6. Strains of this kind are capable of fermenting glucose, galactose and maltose. They use glucose, galactose, maltose, trehalo'z, cellobiose, d-xylose, mannitol and lysine as the sole carbon source. It does not utilize lactose, melibiosis, melezitosis, 1- and d-arabinose, rhamnose, inulin, galactitol, glucitol, arabinitol. Potassium nitrate is not used as a nitrogen source. Ascospores do not form. The maximum growth temperature is 37 ° C. They need vitamins in the environment to grow. They are very osmoresistent, so they grow even in 60% sucrose solution. Cleaves fat and arbutin. These strains are deposited in the Czechoslovak Yeast Collection in Bratislava under CCX 26-19-3,

CCX 26-19-4, CCX 26-19-5, CCX 26-19-7, CCX 26-19-8, CCX 26-19-9, CCX 26-19-10, CCX 26-19-10 26-19-11, CCX 26-19-12, CCX 26-19-13, CCX 26-19-14 β CCX 26-19-15 · The above strains have been tested in experimental animals and have not been shown to be pathogenic or toxic. features. CCX 26-19-7 type culture serves as a typical representative of the whole group. Table 1 shows the relative amino acid composition of the crude protein.

The invention is of importance in agriculture in the use of animal and plant mass production wastes and wood-processing industry wastes for the production of feed biomass.

Table 1

Amino acid composition in crude protein of Torulopsis Kruisii CCX 26-19-7 in mol%

amino acid mol% lysine 7.54 histidine 2.62 arginine 3.85 Aspartic acid 9.03 threonine 6.90

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Continuation of Table 1

amino acid

serine

Glutamic acid Proline

glycine

alanine

valine

1/2 cystine

methionine

leucine

isoleucine

tyrosine

Phenylalanine mol%

7.61 12.88

7.15 7,70

9.55 6.21 f

0.81

7.16

4.56 2.86 3.53

Crude protein (Nx 6.25) was 41.06%,

Example 1 i

Hydrolyzate from swine excrements, resp. other livestock, diluted to 3 to 7% dry matter and to 2 to 5% reducing agents (monosaccharides-hexoses and pentoses), treated with solid calcium hydroxide to pH 5.5 - 6.5, filtered and sterilized. Inoculate with cell suspension for 24 hours. Old culture of the strain Torulopsis kruisii CCY 26-19-7 and grown at 25-30 ° C with ventilation. The biomass yield is converted to the consumption of reducing substances and volatile acids.

Example 2

A hydrolyzate or pre-hydrolyzate from woodworking industrial waste, diluted to a reducing agent content of 2 to 3%, with the addition of 1% ammonium sulphate, adjusted to a pH of 5.5 to 6.5, filtered and sterilized, is used. Inoculated with a suspension of cells of the strain Torulopsis kruisii CCY 26-19-7, grown under ventilation at 25-30 ° C. The biomass yield is converted to the reduction of reducing substances and volatile acids.

Example 3

The hydrolyzate is used as in Example 1 or 2, and after adjusting the pH as in Example 1 or 2, it is shaken with activated charcoal (1 to 3 g per 100 ml cold), filtered and sterilized. Inoculate with the suspension for 24 hours. An old culture of the CCY strain 26-19-7, Torulopsis kruisii, is grown under ventilation at 25-30 ° C. The yield is calculated as the reduction of reducing substances and volatile acids.

Claims (1)

SUBJECT MATTER A method of producing biomass by culturing on a hydrolyzate from animal excrement, or from wastes from the woodworking industry, or hydrolyzate from plant material, characterized in that the hydrolyzate is adjusted to pH 5.5 to 6.5 by addition of solid calcium hydroxide, and a 1% ammonium sulfate is added, the hysirolysate is filtered with activated carbon, inoculated with a strain of Torulopsis kruisii CCY 26-19-7, or CCY 26-19-3, or CCY 26-19-4, or CCY 26-19-5, or CCY 26-19-6, or CCY 26-19-8, or CCY 26-19-9, or CCY 26-19-10, or CCY 26-19-11, or CCY 26-19-12, or CCY 26-19-13, or CCY 26-19-14, or CCY 26-19-15 and is ventilated at 25-30 ° C.