CO6511252A2 - Sistema de extraccion de metales pesados - Google Patents

Sistema de extraccion de metales pesados

Info

Publication number
CO6511252A2
CO6511252A2 CO12046735A CO12046735A CO6511252A2 CO 6511252 A2 CO6511252 A2 CO 6511252A2 CO 12046735 A CO12046735 A CO 12046735A CO 12046735 A CO12046735 A CO 12046735A CO 6511252 A2 CO6511252 A2 CO 6511252A2
Authority
CO
Colombia
Prior art keywords
heavy metal
bacteria
extraction system
provides
gene expression
Prior art date
Application number
CO12046735A
Other languages
English (en)
Inventor
Oscar Ruiz
Original Assignee
Inter America University Of Puerto Rico
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inter America University Of Puerto Rico filed Critical Inter America University Of Puerto Rico
Publication of CO6511252A2 publication Critical patent/CO6511252A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Processing Of Solid Wastes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Removal Of Specific Substances (AREA)

Abstract

La invención proporciona un sistema de secuestro de metal pesado mediante bacterias. La bacteria expresa los genes ppk, mt, y/o P-galactosidasa (lacZ) y puede tolerar por lo menos 25 µM de mercurio, 1,000 µM de zinc, 250 µM de cadmio, y 3,000 µM de Pb. El sistema permite la determinación fácil de la presencia de contaminantes de metal pesado en un líquido y la recolección fácil de la bacteria que ha secuestrado grandes cantidades de metal pesado. Se proporciona adicionalmente un sistema de expresión de gen en bacterias que comprende elementos de expresión de gen de fago y plástido y suministra un nivel de expresión de proteína particularmente alto y resistencia al metal pesado.
CO12046735A 2009-08-20 2012-03-20 Sistema de extraccion de metales pesados CO6511252A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23562409P 2009-08-20 2009-08-20

Publications (1)

Publication Number Publication Date
CO6511252A2 true CO6511252A2 (es) 2012-08-31

Family

ID=43607596

Family Applications (1)

Application Number Title Priority Date Filing Date
CO12046735A CO6511252A2 (es) 2009-08-20 2012-03-20 Sistema de extraccion de metales pesados

Country Status (14)

Country Link
US (2) US8785174B2 (es)
EP (1) EP2467486B1 (es)
JP (1) JP2013502223A (es)
KR (1) KR101812629B1 (es)
CN (2) CN102753691B (es)
AU (1) AU2010284144B2 (es)
BR (1) BR112012003726A2 (es)
CA (1) CA2771528C (es)
CL (1) CL2012000436A1 (es)
CO (1) CO6511252A2 (es)
IL (1) IL218196A (es)
NZ (1) NZ598789A (es)
RU (1) RU2571222C2 (es)
WO (1) WO2011022590A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5660508B2 (ja) 2010-04-08 2015-01-28 国立大学法人旭川医科大学 腸管保護剤
CN103525849B (zh) * 2013-10-24 2016-01-20 舒海燕 一种用于清除工业废水汞污染的重组质粒、构建方法、重组工程菌及应用
CN104445845B (zh) * 2014-10-31 2016-05-25 杭州师范大学 一种快速缓解重金属对厌氧氨氧化污泥活性抑制的方法
CN106630494B (zh) * 2015-07-26 2021-10-29 刘兴全 一种去除含镉污水污染物的方法
US20170157013A1 (en) * 2015-12-07 2017-06-08 Colgate-Palmolive Company Metal Amino Acid Complexes for Bacterial Aggregation
CN107916243A (zh) * 2016-10-10 2018-04-17 中国科学院微生物研究所 用于处理重金属污染的微生物细胞和蛋白、相应的处理方法和试剂盒
US10759683B2 (en) * 2018-07-10 2020-09-01 University Of Ottawa Methods for removing mercury contaminant from aqueous solutions, and bioreactors therefor
CN109943571B (zh) * 2019-04-11 2022-09-02 中国科学院遗传与发育生物学研究所农业资源研究中心 一种金属硫蛋白基因mt20、其编码得到的金属硫蛋白及其表达和应用
CN110819551B (zh) * 2019-07-02 2023-04-21 南阳师范学院 一株可阻控叶菜类蔬菜富集Cd和Pb的布甘氏肠杆菌CH6及其用途
AU2021341795B2 (en) 2020-09-08 2024-02-01 Frederick William Macdougall Coalification and carbon sequestration using deep ocean hydrothermal borehole vents
US11794893B2 (en) 2020-09-08 2023-10-24 Frederick William MacDougall Transportation system for transporting organic payloads
CN112961794B (zh) * 2021-01-19 2023-01-03 西安中地环境科技有限公司 一种吸附汞的复合菌制剂及应用
CN114181841A (zh) * 2021-11-24 2022-03-15 浙江大学 治理汞污染的真菌、生物菌剂及应用和除汞方法以及鉴定治理汞污染能力真菌的方法
CN114231459B (zh) * 2021-12-27 2023-05-26 中南大学 一种大肠埃希氏菌、微生物菌剂、组合物及应用
CN114152601B (zh) * 2021-12-28 2023-09-15 军事科学院军事医学研究院环境医学与作业医学研究所 一种现场快速检测水中汞离子的方法、试剂盒及其应用
PL443378A1 (pl) * 2022-12-31 2024-07-01 Instytut Biotechnologii I Medycyny Molekularnej 7 aminokwasowe sekwencje oraz klony fagowe wiążące selektywnie jony niklu
CN116656586A (zh) * 2023-03-17 2023-08-29 成都中医药大学 金属硫蛋白表面展示菌在川芎镉污染防治中的应用的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003011487A1 (en) * 2001-07-27 2003-02-13 Global Biosciences, Inc. Remediation of metal contaminants with hydrocarbon-utilizing bacteria
US8440791B2 (en) * 2001-09-06 2013-05-14 Mgp Biotechnologies, Llc Thimerosal removal device
RU2224604C1 (ru) * 2002-06-27 2004-02-27 Шулаева Раиса Васильевна Способ ремедиации нефтезагрязненных грунтов
WO2006060769A2 (en) * 2004-12-03 2006-06-08 Xoma Technology Ltd. Methods and materials for expression of a recombinant protein
BRPI0907501B1 (pt) * 2008-02-01 2021-02-23 Ceres, Inc construto de vetor, método para dirigir a transcrição, método de expressão de uma região de codificação exógena, método de alteração da expressão de um gene e método de produção de uma planta transgênica

Also Published As

Publication number Publication date
WO2011022590A3 (en) 2011-07-28
RU2571222C2 (ru) 2015-12-20
KR20120064078A (ko) 2012-06-18
AU2010284144A1 (en) 2012-04-12
US20140273161A1 (en) 2014-09-18
CN102753691B (zh) 2014-05-14
KR101812629B1 (ko) 2017-12-27
CN103992980A (zh) 2014-08-20
RU2012110039A (ru) 2013-09-27
CN102753691A (zh) 2012-10-24
EP2467486A2 (en) 2012-06-27
EP2467486B1 (en) 2016-04-06
AU2010284144B2 (en) 2015-07-23
IL218196A (en) 2016-05-31
IL218196A0 (en) 2012-04-30
US9719096B2 (en) 2017-08-01
BR112012003726A2 (pt) 2017-07-04
EP2467486A4 (en) 2013-06-12
CA2771528A1 (en) 2011-02-24
CL2012000436A1 (es) 2013-03-08
US20110220570A1 (en) 2011-09-15
WO2011022590A2 (en) 2011-02-24
NZ598789A (en) 2013-12-20
US8785174B2 (en) 2014-07-22
CA2771528C (en) 2018-10-30
JP2013502223A (ja) 2013-01-24

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