CO6030033A1 - A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBES - Google Patents
A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBESInfo
- Publication number
- CO6030033A1 CO6030033A1 CO08025973A CO08025973A CO6030033A1 CO 6030033 A1 CO6030033 A1 CO 6030033A1 CO 08025973 A CO08025973 A CO 08025973A CO 08025973 A CO08025973 A CO 08025973A CO 6030033 A1 CO6030033 A1 CO 6030033A1
- Authority
- CO
- Colombia
- Prior art keywords
- hpv16
- primers
- dna
- detection
- identification
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método para la detección e identificación de variantes en E6 HPV16, que comprende los pasos de: (a) Tener una muestra biológica que se sospeche tiene infección por HPV16. (b) Proporcionar un par de primers bidireccionales dirigidos hacia partes específicas en la región E6 de HPV16 que amplifican un fragmento de 562 pb; (c) Realización de una reacción para amplificar el DNA derivado de la muestra usando el par de primers; (d) detección del DNA de HPV 16 de los productos de la amplificación de la muestra; (e) identificación específica de 73 variantes diferentes en el gen E6 HPV16. Un método para la detección e identificación de variantes en E6 HPV16, que comprende los pasos de: (a) Tener una muestra biológica que se sospeche tiene infección por HPV16; (b) Proporcionar una diversidad de primers bidireccionales internos dirigidos hacia partes específicas en la región E6 que amplifican fragmentos de 367pb, 362pb y 167pb del DNA de E6 de HPV16 (c) Realización de diferentes reacciones de amplificación para amplificar el DNA derivado de la muestra usando la diversidad de primers; y (d) detección del DNA de HPV16 de los productos de la amplificación de la muestra; (e) identificación específica de 73 variantes diferentes en el gen E6 HPV16. Un método de acuerdo a las reivindicaciones 1 y 2, en donde los pares de primers bidireccionales comprenden primers que son colectivamente complementarios a una primera región consenso del DNA de E6 de HPV16, y los cuales además comprenden primers que son colectivamente substancialmente complementarios a una segunda región consenso del DNA de E6 de HPV16. Un método de acuerdo a la reivindicación 3, en donde la primera y segunda regiones consenso están en el ORF E6 de HPV 16. Un método de acuerdo a la reivindicación 4, en donde los primers están descritos y definidos en la FIG.1. Un método de acuerdo a la reivindicación 5, en donde uno de cada par de primers es marcado con biotina Un método de acuerdo a las reivindicaciones 1 y 2 en donde el paso (d) se lleva a cabo por la hibridización de los productos amplificados con una diversidad de sondas específicas de E6 de HPV16. Un método de acuerdo a la reivindicación 7, donde las sondas específicas son complementarias a secuencias de DNA de E6 HPV16 entre el nucleótido 28 y el nucleótido 580 de E6 de HPV16.A method for the detection and identification of variants in E6 HPV16, which comprises the steps of: (a) Having a biological sample suspected of having HPV16 infection. (b) Provide a pair of bidirectional primers directed towards specific parts in the E6 region of HPV16 that amplify a 562 bp fragment; (c) Performing a reaction to amplify the DNA derived from the sample using the pair of primers; (d) HPV 16 DNA detection of the sample amplification products; (e) specific identification of 73 different variants in the E6 HPV16 gene. A method for the detection and identification of variants in E6 HPV16, which comprises the steps of: (a) Having a biological sample suspected of having HPV16 infection; (b) Provide a variety of internal bidirectional primers directed towards specific parts in the E6 region that amplify fragments of 367pb, 362pb and 167pb of the HP6 E6 DNA (c) Performing different amplification reactions to amplify the sample derived DNA using the diversity of primers; and (d) HPV16 DNA detection of the sample amplification products; (e) specific identification of 73 different variants in the E6 HPV16 gene. A method according to claims 1 and 2, wherein the pairs of bidirectional primers comprise primers that are collectively complementary to a first consensus region of the HPV16 E6 DNA, and which further comprise primers that are collectively substantially complementary to a second E6 DNA consensus region of HPV16. A method according to claim 3, wherein the first and second consensus regions are in the ORV E6 of HPV 16. A method according to claim 4, wherein the primers are described and defined in FIG. 1. A method according to claim 5, wherein one of each pair of primers is labeled with biotin A method according to claims 1 and 2 wherein step (d) is carried out by hybridization of the products amplified with a variety of specific E6 probes of HPV16. A method according to claim 7, wherein the specific probes are complementary to DNA sequences of E6 HPV16 between nucleotide 28 and nucleotide 580 of E6 of HPV16.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CO08025973A CO6030033A1 (en) | 2008-03-12 | 2008-03-12 | A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CO08025973A CO6030033A1 (en) | 2008-03-12 | 2008-03-12 | A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6030033A1 true CO6030033A1 (en) | 2009-04-30 |
Family
ID=40981980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO08025973A CO6030033A1 (en) | 2008-03-12 | 2008-03-12 | A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBES |
Country Status (1)
| Country | Link |
|---|---|
| CO (1) | CO6030033A1 (en) |
-
2008
- 2008-03-12 CO CO08025973A patent/CO6030033A1/en not_active Application Discontinuation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AR110776A2 (en) | IN VITRO METHOD FOR THE SIMULTANEOUS DETECTION AND DISCRIMINATION OF PATHOGENS | |
| Belák | Molecular diagnosis of viral diseases, present trends and future aspects: A view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine | |
| RU2013148806A (en) | METHODS AND KITS FOR DETECTION OF CELL-FREE CELLS SPECIFIC FOR PATHOGENES OF NUCLEIC ACIDS | |
| NZ601652A (en) | Set of probes for the detection and typing of 46 human papillomavirus mucosal types | |
| FI2831279T3 (en) | Rapid aneuploidy detection | |
| ES2896198T3 (en) | Polymerase Chain Reaction Primers and Probes for Mycobacterium tuberculosis | |
| Zhang et al. | Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches | |
| AR122413A2 (en) | METHOD TO IDENTIFY A DNA SEQUENCE THAT CONTRIBUTES TO RESISTANCE TO CABBAGE HERNIA DISEASE IN A BRASSICA NAPUS PLANT | |
| BRPI0816047A2 (en) | oligonucleotide array for detection of a target nucleic acid, methods for detecting the presence or absence of a target nucleic acid and at least two target nucleic acids, kit, reaction mixture and fluorescence-identified amplicon | |
| KR101712451B1 (en) | Method for detecting food borne pathogens using digital PCR | |
| Xu et al. | Rapid detection of measles virus using reverse transcription loop-mediated isothermal amplification coupled with a disposable lateral flow device | |
| WO2009061640A3 (en) | Hepatitis b virus (hbv) specific oligonucleotide sequences | |
| AR090558A1 (en) | CORN EVENT DP-004114-3 AND METHODS FOR DETECTION | |
| Gazali et al. | Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis | |
| WO2013156432A1 (en) | Hev assay | |
| RU2612137C1 (en) | Method for identification of tularemia pathogen subspecies francisella tularensis subsp tularensis, francisella tularensis subsp mediasiatica and francisella tularensis subsp holarctica | |
| RU2558236C2 (en) | Analysis system for detection of closely related serotypes of human papilloma virus (hpv) | |
| DE602005023002D1 (en) | HYBRIDIZATION PROCEDURE | |
| CO6030033A1 (en) | A METHOD FOR THE DETECTION AND IDENTIFICATION OF HPV16 VARIANTS THROUGH THE USE OF PRIMERS AND PROBES | |
| CA2829852C (en) | Dual probe assay for the detection of hcv | |
| ZA202305277B (en) | Primers, kit and method for detecting of african swine fever virus | |
| EA201792628A3 (en) | METHOD OF SPECIFIC IDENTIFICATION OF DNA SEQUENCE SPECIFICATIONS | |
| JP2013534810A5 (en) | ||
| RU2013158142A (en) | DIAGNOSIS OF HEPATITIS TTV VIRUS | |
| IN2014DN07968A (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Application refused |