CO2022001738A1 - Compositions and methods to detect sars-cov-2 in human clinical samples - Google Patents

Compositions and methods to detect sars-cov-2 in human clinical samples

Info

Publication number
CO2022001738A1
CO2022001738A1 CONC2022/0001738A CO2022001738A CO2022001738A1 CO 2022001738 A1 CO2022001738 A1 CO 2022001738A1 CO 2022001738 A CO2022001738 A CO 2022001738A CO 2022001738 A1 CO2022001738 A1 CO 2022001738A1
Authority
CO
Colombia
Prior art keywords
cov
compositions
methods
human
clinical samples
Prior art date
Application number
CONC2022/0001738A
Other languages
Spanish (es)
Inventor
Tiria Felix Giovanni Delgado
Parra Jaime Eduardo Castellanos
Tapiero Eliana Patricia Calvo
Mendoza Camilo Andrés Castellar
Ruiz Carolina Coronel
Ibarra Lady Stephanie López
Cepeda Lilia Jadith Bernal
Peláez María Angélica Calderón
Romero Myriam Lucía Velandia
Ávila Sonia Del Pilar Bohórquez
Barbosa Héctor Hernando Gutiérrez
Jhann Andrés Arturo
Ortega Sigrid Johanna Camacho
Original Assignee
Univ El Bosque
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ El Bosque filed Critical Univ El Bosque
Priority to CONC2022/0001738A priority Critical patent/CO2022001738A1/en
Publication of CO2022001738A1 publication Critical patent/CO2022001738A1/en
Priority to PCT/IB2022/058734 priority patent/WO2023156837A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

[40] La presente invención hace referencia a un método para detectar la presencia del virus del síndrome respiratorio agudo severo asociado virus (SARS-CoV-2) en una muestra biológica por transcripción reversa acoplada a la reacción en cadena de la polimerasa (RT-PCR) en tiempo real, a partir de conjuntos de cebadores y sondas que permiten la amplificación simultánea de dos genes virales y un gen humano de control en una misma reacción. El método permite realizar un diagnóstico in vitro de COVID19 a partir de muestras biológicas humanas, preferiblemente muestras del tracto respiratorio humano como hisopados nasofaríngeos.[40] The present invention refers to a method for detecting the presence of the severe acute respiratory syndrome virus associated virus (SARS-CoV-2) in a biological sample by reverse transcription coupled to the polymerase chain reaction (RT- PCR) in real time, from sets of primers and probes that allow the simultaneous amplification of two viral genes and a human control gene in the same reaction. The method allows for an in vitro diagnosis of COVID19 from human biological samples, preferably human respiratory tract samples such as nasopharyngeal swabs.

CONC2022/0001738A 2022-02-18 2022-02-18 Compositions and methods to detect sars-cov-2 in human clinical samples CO2022001738A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CONC2022/0001738A CO2022001738A1 (en) 2022-02-18 2022-02-18 Compositions and methods to detect sars-cov-2 in human clinical samples
PCT/IB2022/058734 WO2023156837A1 (en) 2022-02-18 2022-09-15 Compositions and methods for detecting sars-cov-2 in human clinical samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CONC2022/0001738A CO2022001738A1 (en) 2022-02-18 2022-02-18 Compositions and methods to detect sars-cov-2 in human clinical samples

Publications (1)

Publication Number Publication Date
CO2022001738A1 true CO2022001738A1 (en) 2022-04-19

Family

ID=81127788

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2022/0001738A CO2022001738A1 (en) 2022-02-18 2022-02-18 Compositions and methods to detect sars-cov-2 in human clinical samples

Country Status (2)

Country Link
CO (1) CO2022001738A1 (en)
WO (1) WO2023156837A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210277488A1 (en) * 2020-03-05 2021-09-09 Hackensack Meridian Health, Inc. CDI Enhanced COVID-19 Test

Also Published As

Publication number Publication date
WO2023156837A9 (en) 2024-06-13
WO2023156837A1 (en) 2023-08-24

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