CO2022001738A1 - Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas - Google Patents

Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas

Info

Publication number
CO2022001738A1
CO2022001738A1 CONC2022/0001738A CO2022001738A CO2022001738A1 CO 2022001738 A1 CO2022001738 A1 CO 2022001738A1 CO 2022001738 A CO2022001738 A CO 2022001738A CO 2022001738 A1 CO2022001738 A1 CO 2022001738A1
Authority
CO
Colombia
Prior art keywords
cov
compositions
methods
human
clinical samples
Prior art date
Application number
CONC2022/0001738A
Other languages
English (en)
Inventor
Tiria Felix Giovanni Delgado
Parra Jaime Eduardo Castellanos
Tapiero Eliana Patricia Calvo
Mendoza Camilo Andrés Castellar
Ruiz Carolina Coronel
Ibarra Lady Stephanie López
Cepeda Lilia Jadith Bernal
Peláez María Angélica Calderón
Romero Myriam Lucía Velandia
Ávila Sonia Del Pilar Bohórquez
Barbosa Héctor Hernando Gutiérrez
Jhann Andrés Arturo
Ortega Sigrid Johanna Camacho
Original Assignee
Univ El Bosque
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ El Bosque filed Critical Univ El Bosque
Priority to CONC2022/0001738A priority Critical patent/CO2022001738A1/es
Publication of CO2022001738A1 publication Critical patent/CO2022001738A1/es
Priority to PCT/IB2022/058734 priority patent/WO2023156837A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

[40] La presente invención hace referencia a un método para detectar la presencia del virus del síndrome respiratorio agudo severo asociado virus (SARS-CoV-2) en una muestra biológica por transcripción reversa acoplada a la reacción en cadena de la polimerasa (RT-PCR) en tiempo real, a partir de conjuntos de cebadores y sondas que permiten la amplificación simultánea de dos genes virales y un gen humano de control en una misma reacción. El método permite realizar un diagnóstico in vitro de COVID19 a partir de muestras biológicas humanas, preferiblemente muestras del tracto respiratorio humano como hisopados nasofaríngeos.
CONC2022/0001738A 2022-02-18 2022-02-18 Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas CO2022001738A1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CONC2022/0001738A CO2022001738A1 (es) 2022-02-18 2022-02-18 Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas
PCT/IB2022/058734 WO2023156837A1 (es) 2022-02-18 2022-09-15 Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CONC2022/0001738A CO2022001738A1 (es) 2022-02-18 2022-02-18 Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas

Publications (1)

Publication Number Publication Date
CO2022001738A1 true CO2022001738A1 (es) 2022-04-19

Family

ID=81127788

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2022/0001738A CO2022001738A1 (es) 2022-02-18 2022-02-18 Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas

Country Status (2)

Country Link
CO (1) CO2022001738A1 (es)
WO (1) WO2023156837A1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210277488A1 (en) * 2020-03-05 2021-09-09 Hackensack Meridian Health, Inc. CDI Enhanced COVID-19 Test

Also Published As

Publication number Publication date
WO2023156837A9 (es) 2024-06-13
WO2023156837A1 (es) 2023-08-24

Similar Documents

Publication Publication Date Title
CN111074011B (zh) 检测引起呼吸道感染的病毒并分型的组合物、试剂盒、方法及其用途
Meir et al. Development of a real-time TaqMan® RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation
JP6329370B2 (ja) 呼吸器系ウイルスによる疾患の同時診断キット
Acevedo et al. A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus
US20090226888A1 (en) Diagnostic Primers And Method For Detecting Avian Influenza Virus Subtype H5 And H5N1
CN101899534B (zh) 用于检测小鹅瘟病毒和番鸭细小病毒的试剂盒及其检测方法
CN106555012A (zh) 用于a型动物流感病毒检测的试剂、检测方法及应用
WO2018035860A1 (zh) 同时检测及定量分析四种血源性病毒的多重Taqman探针qPCR的试剂盒及方法
US20140370496A1 (en) Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method
Yin et al. Visual detection of duck Tembusu virus with CRISPR/Cas13: A sensitive and specific point-of-care detection
CO2022001738A1 (es) Composiciones y métodos para detectar sars-cov-2 en muestras clínicas humanas
RU2761496C2 (ru) Способ идентификации вируса репродуктивно-респираторного синдрома свиней на основе ПЦР в реальном времени
Jia et al. Detection of a novel highly pathogenic H7 influenza virus by duplex real-time reverse transcription polymerase chain reaction
Padilha de Fraga et al. A real-time reverse-transcription polymerase chain reaction for differentiation of Massachusetts vaccine and brazilian field genotypes of avian infectious bronchitis virus
CN106086238A (zh) 猪圆环病毒2型的pcr检测方法、所用引物及检测试剂盒
RU2515911C1 (ru) Набор олигодезоксирибонуклеотидных праймеров и флуоресцентно-меченых зондов для идентификации рнк и дифференциации вируса парагриппа человека 1, 2, 3 и 4 типов
CN104164518A (zh) 一种直接检测a+b型流感病毒的实时荧光rt-pcr方法
TWI711701B (zh) 用於檢測禽流感病毒及其演化分支病毒亞型之引子組及方法
CN108411042A (zh) 一种检测乙型脑炎病毒的荧光定量pcr引物及试剂盒
Yang et al. Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells
KR101617142B1 (ko) 개선된 검출 정확성을 제공해 줄 수 있는 핵산검사 기반의 조류 인플루엔자 바이러스 검출 키트
RU2615465C2 (ru) Диагностическая система для выявления днк провирусов лейкоза и иммунодефицита крупного рогатого скота методом мультиплексной полимеразной цепной реакции
WO2016078215A1 (zh) 一步法反转录pcr检测和分型埃博拉病毒5种亚型的引物、探针及试剂盒
CN106435030B (zh) 一种联合检测禽流感h7n9和h9n2的四通道荧光定量pcr检测方法及试剂盒
CN113025746B (zh) 利用nasba检测rev的方法及使用的成套试剂