CN85200106U - Chemiluminescence analyzer for detecting pesticides e.g. dichlorvos - Google Patents
Chemiluminescence analyzer for detecting pesticides e.g. dichlorvos Download PDFInfo
- Publication number
- CN85200106U CN85200106U CN 85200106 CN85200106U CN85200106U CN 85200106 U CN85200106 U CN 85200106U CN 85200106 CN85200106 CN 85200106 CN 85200106 U CN85200106 U CN 85200106U CN 85200106 U CN85200106 U CN 85200106U
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- CN
- China
- Prior art keywords
- dichlorvos
- analyzer
- darkroom
- luminol
- chemical
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model belongs to the technical field of practical analysis. The usual enzyme inhibition method for detecting pesticides e.g. dichlorvos has bad repetitiveness, and the reagent can not be easily preserved. The development of the utility model is used for a chemical luster analyzer for detecting pesticides e.g. dichlorvos. The reaction liquid in the analyzer is composed of Luminol mixed liquor and the measured agricultural chemical water sample. When the Luminol mixed liquor mixes with the agricultural chemical water sample, and the chemical luster can be generated. The analyzer through detecting the intensity of the chemical luster to detect the concentration of micro dichlorvos, phosphamidon, monocrotophos and other organic pesticide in water.
Description
The invention belongs to the proximate analysis technical field.
Existing light analyzer partly is made up of darkroom, photomultiplier, register system etc., and the detection method of organophosphorus pesticides such as existing DDVP is that enzyme suppresses method, this method repeated bad, and enzyme reagent is difficult for preservation.The objective of the invention is to develop a kind of instrument of new agricultural chemicals such as detection DDVP.Its solution is that phosphoric acid alkene ester pesticides such as DDVP can produce blue green chemical light with the effect of luminol mixed liquor, and good linear relationship is arranged between light intensity and pesticide concentration.According to this principle design chemical light analyzer.
This instrument is by reaction darkroom, luminol mixed liquor injection port, and photomultiplier system, register system etc. are partly formed.Cuvette in the darkroom can be 25ml, and injection port is the device of an isolated external light source, is contained on the reaction darkroom, and this device can be to deceive moulds pipe, is about 25Cm, links to each other with cassette cover, can completely cut off extraneous light and enter the reaction darkroom.Its head can be done funneling, so that solution adds, this sampling device can add the luminol mixed solution under the situation of not opening the reaction darkroom.Adopt simple and easy high-voltage power supply to provide operating voltage for the photomultiplier in the photomultiplier system.Registering instrument is common X-Y recorder.
During test sample, cutoff high power supply at first, power light goes out, and gets agricultural chemicals water sample 5ml and puts in the cuvette darkroom of closing, open high-voltage power supply and make registering instrument be in the paper feed state, luminol mixed liquor 5ml is added from reaction darkroom injection port, and record light curve is measured peak height, linear between peak height and sample concentration, the range of linearity is mainly determined by the linear working range of photomultiplier.This apparatus structure is simple, highly sensitive, can write down its light intensity and can provide complete light curve in the moment that sample and luminol mixed liquor begin to act on.Compare with existing liquid scintillation light analyzer, have cheap, the characteristics of stable performance, but adopt in this analyzer quantitative measurement water the concentration of the phosphoric acid alkene ester pesticides such as DDVP, phosphamidon, Azodrin of trace.
This instrument can be further specified by accompanying drawing:
Fig. 1 is simple and easy high-voltage power supply electrical schematic diagram, civil power can be transformed into 1000 volts～1500 volts high direct voltage.
Fig. 2 is the photomultiplier circuit.
Fig. 3 is chemical light analyzer structural drawing.Wherein 1 is luminol mixed liquor injection port, and 2 are reaction darkroom lid, and 3 are the reaction darkroom, and 4 is cuvette, 5 is the photomultiplier detection window, and 6 is photomultiplier, and 7 is high-voltage power supply, and 8 is power light, 9 is power switch, and 10 is the signal output plughole, connects X-Y recorder, and 11 are supply socket.
In this instrument, best luminol mixed liquor is made up of solution I and II:
Solution I: contain 2.5mM luminol; 0.025MNa
3PO
45MNaCl; 0.3mM EDTA.
Solution II: 0.1M sodium perborate.
Solution I and II mixed to place behind the Uniform by the 100:6 volume ratio be the luminol mixed solution after 1 hour.This solution should use preceding preparation, and is unsuitable stored for a long time.The solution I can be deposited half a year, and the solution II can be deposited in refrigerator a week.
Method above adopting, it is 0.02 μ g/ml that DDVP detects sensitivity, when concentration during greater than 2 μ g/ml, because light intensity is very big, so must the employing optical attenuation technologies measure or sample is made dilution process.The sensitivity of phosphamidon, Azodrin is 0.1 μ g/ml, and the range of linearity is greater than 0～100 μ g/ml.According to the shape of light curve, can qualitative differentiation DDVP and metrifonate.
Claims (2)
1, a kind of chemical light analyzer of forming by darkroom, photomultiplier, register system.Characteristics of the present invention are without power supply, place the cylindrical cuvette that fills the luminol mixed liquor in the darkroom.The injection port of isolated extraneous light is housed on the reaction darkroom.
2, according to claim 1, injection port is a black pipe of moulding that links to each other with the reaction darkroom, and head is made funnel type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85200106 CN85200106U (en) | 1985-04-01 | 1985-04-01 | Chemiluminescence analyzer for detecting pesticides e.g. dichlorvos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85200106 CN85200106U (en) | 1985-04-01 | 1985-04-01 | Chemiluminescence analyzer for detecting pesticides e.g. dichlorvos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN85200106U true CN85200106U (en) | 1986-04-09 |
Family
ID=4796522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 85200106 Ceased CN85200106U (en) | 1985-04-01 | 1985-04-01 | Chemiluminescence analyzer for detecting pesticides e.g. dichlorvos |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN85200106U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198386A (en) * | 2015-05-07 | 2016-12-07 | 苏州晋翌生物医学仪器有限公司 | Biochemical reaction device and biochemical reaction system |
-
1985
- 1985-04-01 CN CN 85200106 patent/CN85200106U/en not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198386A (en) * | 2015-05-07 | 2016-12-07 | 苏州晋翌生物医学仪器有限公司 | Biochemical reaction device and biochemical reaction system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CE01 | Termination of patent right | ||
| CE01 | Termination of patent right |
Termination date: 19861022 |