CN2923719Y

CN2923719Y - Tumor-cell deep low temperature long-time cold-storage system

Info

Publication number: CN2923719Y
Application number: CNU2005200436178U
Authority: CN
Inventors: 高春平
Original assignee: Individual
Current assignee: Individual
Priority date: 2005-07-21
Filing date: 2005-07-21
Publication date: 2007-07-18
Anticipated expiration: 2015-07-21

Abstract

The utility model relates to a biomedical experimental device, in particular to a biomedical laboratory device for deep low temperature long-term cold storage of the tumor cell. More concretely speaking, the utility model is a deep low temperature long-term cold storage system for the tumor cell, which comprises a deep low temperature cold storage tube, a transportation packing box, a swinging constant temperature incubator, a tube substructure in the incubator, a cell cleaning concentration device, a managing system, a liquid nitrogen container and a deep low temperature freezing box. The utility model is specially used in acquisition, transportation, treatment and deep low temperature cold storage for tumor cells.

Description

The long-term reefer system of tumour cell profound hypothermia

Affiliated technical field

The utility model relates to a kind of biomedicine experiment device, relates in particular to a kind of biomedical laboratory's device that is applied to the long-term refrigeration of tumour cell profound hypothermia.More particularly, this is a kind ofly to clean concentrating unit, management system, liquid nitrogen vessel, profound hypothermia by test tube flat, cell in profound hypothermia refrigeration test tube, Transport Package box, swing property constant incubator, the incubator and freeze the long-term reefer system of tumour cell profound hypothermia that case is formed, is specifically applied to tumour cell collection, transportation, processing, profound hypothermia and refrigerates.

Background technology

The clinical effectiveness of using dendritic cell vaccination treatment tumour and tumor vaccine treatment tumour is confirmed by a lot of preclinical medicine experiments and clinical medicine test, the polytype tumour of dendritic cell and vaccine therapy has successfully been used in a lot of American-European medical centers, and has obtained the success that attracts people's attention.

The basic premise of using dendritic cell and tumor vaccine treatment tumour is to gather patient self vivo tumor cell in tumor resection, when endoscopic biopsy, through the external paracentesis of skin the time, carry out long-time profound hypothermia and preserve, use patient self tumour cell and prepare all kinds of tumor vaccines.

Still do not have report application specific equipment at present and finish above-mentioned task, clinical labororatory's custom is continued to use the breadboard common used material of common biology, device and equipment and is gathered, transports, handles, preserves tumour cell at present, various problems such as often take place that sample pollutions, sample number administration confusion, sample are not good at certainly, especially when clinical profound hypothermia in enormous quantities refrigerated a plurality of patients' sample, the problem performance was particularly outstanding.

Summary of the invention

In order to remedy prior art defective and deficiency, the purpose of this utility model provides a kind of novel long-term reefer system of tumour cell profound hypothermia that is specifically designed to tumour cell collection, transportation, processing and profound hypothermia refrigeration, more efficient and preservation tumour cell more in high quality.

In order to solve its technical problem, the technical solution that the utility model adopts, it is characterized in that, this is a kind ofly to clean concentrating unit, management system, liquid nitrogen vessel, profound hypothermia by test tube flat, cell in profound hypothermia refrigeration test tube, Transport Package box, swing property constant incubator, the incubator and freeze the long-term reefer system of tumour cell profound hypothermia that case is formed, is specifically applied to tumour cell collection, transportation, processing and profound hypothermia and refrigerates.Feature of the present utility model is that its device is made of following part:

● profound hypothermia refrigeration test tube

The ■ test tube: can use non-toxic plastic, as polypropylene etc., or adopt metallic substance, stainless steel must the ability profound hypothermia one 198 ℃, and its upper end band outside nut cooperates with the test tube lid.

■ test tube lid: be used for sealing test tube, the band internal thread, and match with screw in vitro and to be connected, test tube has covered a hole, be used for fixing pipeline with extraneous traffic, closed and fixed with silicone rubber gasket between test tube lid hole and the pipeline, the test tube lid also can be made with metal and plastics, must anti-profound hypothermia.

■ and the extraneous pipeline that has a common boundary: fixing by silicone rubber gasket and test tube lid, its top has outside screw to link to each other with cap, and available metal or non-toxic plastic are made.

The cap of ■ and extraneous traffic pipeline: sealing test tube and extraneous traffic have inside screw to cooperate with pipeline.

■ silicone rubber gasket-restrained line and test tube are formed with medical silica-gel.

All material all can be made of stainless material during ■ trial-production, as sample.Can consider to use the mould of plastics batch machining during production in enormous quantities.

■ test tube volume should can hold enough tumor tissues about 10ml.

● the Transport Package box

37 ℃ of thermostats are arranged, and battery is chargeable, free record, temperature alarming, lagging material.

● the swing type constant incubator

Can use matured product, add configuration as requested, require 37 ℃ of constant temperature, regularly, temperature shows, reports to the police, can be used as and hatched (collagenase) or deactivation tumour cell (41 ℃) two hours.

● test tube flat in the incubator.

Be used for fixing test tube, profile matches with test tube, and timing, thermometric, warning (acousto-optic) function are arranged, and can stably be fixed in the incubator, be used to hatch regularly, or do not have and live regularly, digitalizer, can the design temperature time of fire alarming regularly, and sound and light alarm is arranged.

● cell cleans concentrating unit.

Typical case TFF device adopts 0.45 μ m filtering membrane, peristaltic pump speed 400ml/min, and enrichment factor 1: 10, the refrigeration test tube is upside down on the test-tube stand.

The TFF container can have side mouth, suitable for reading, end opening with glass as material, can use repeatedly, and the TFF strainer also can use after cleaning, sterilizing repeatedly, and the TFF container adopts the 50ml initial capacity.

Every covering device needs only a peristaltic pump and control device, and TFF container and filter cartridge be each patient's special use then.

● management system

Adopt LIMS software.Adopt LIMS software, carry out self-customization definition, network management according to tumour cell refrigeration keeping characteristics.

● the tumor cell lysate preparation facilities.

■ ultrasonic wave tumour antigen preparation facilities.

With refrigeration test tube corollary apparatus, ultrasonic transducer places the test tube flat bottom, and ultrasonic-frequency power supply places the bottom equally, and the ultrasonic energy and the time of process test back regulation can broken tumour cells.

■ frozen-thawed cell cracker

Special refrigeration test tube base, free automatic setting device is put into the profound hypothermia refrigerator behind the setting-up time, after specified time arrives, report to the police automatically, the technician puts it into 37 ℃ of constant water bath box again, time is set before the placement equally, reports to the police automatically again after the time arrives, lysis after continuous 3 times.

The utility model device can be widely used in following purposes:

■ sets up chain tumour cell bank

■ provides tumor vaccine preparation service for patient

■ provides tumour antigen preparation service for patient

■ preserves the fresh and alive cell of other various human bodies for patient

■ sets up chain " tissue and cell bank "

The beneficial effects of the utility model are, propose a kind of automatic management, be specifically applied to the automated installation of tumour cell cryopreservation, improve the efficient that tumour cell preserves and improve the keeping quality.

Description of drawings

The utility model is described in further detail below in conjunction with drawings and Examples

Fig. 1: profound hypothermia refrigeration test tube synoptic diagram

Fig. 2: tumour cell profound hypothermia reefer system synoptic diagram

Fig. 3: tumour cell cracking preparation facilities synoptic diagram

Among the figure, 101 silicone rubber gaskets, 102 test tubes lids (band internal thread), 103 extension tube attached block, 104 extension tube attached, 105 test tubes, 201 damping fluids, 202 refrigeration test tubes, 203 metal filter screens, 204TFF box, 205 peristaltic pumps, 206 test tubes, 207 test-tube stands, 208 abandon the liquid bag, 209 side mouths, 210 is suitable for reading, 211 end openings, in 212 filtering outlets, 213 inlets, 214 outlets, 301 test tubes, 302 test-tube stands, 303 ultrasonic transducers, 304 ultrasonic powers.

Embodiment

1. gather patient's tumor tissues

■ under the gnotobasis, gathers aseptic, fresh and alive tumor tissues at Operation theatre or Endoscopy Room

Have in the test kit of ■ configuration:

◆ the nutrient solution (being used for cleaning and sterilizing) of band antibiotic, tumor tissues place and discarded behind the cleaning and sterilizing in inner several minutes.

◆ little scissors.Tumor tissues is cut into 1mm ³Following fragment, and carefully choose except that fiber, blood vessel.

◆ refrigeration test tube (in vitro placing 8～10ml nutrient solution).

After the tumor tissues that ■ has shredded into fritter is put into the refrigeration test tube, put into special transport container (behind the nutrient solution cleaning and sterilizing number of band antibiotic minute).

■ is provided with temperature, sets acquisition time.

■ must label on refrigeration test tube and transport case.

2. transportation

Should be transported to specified processing place in 24 hours, if haulage time is longer, the cap that then refrigerates test tube adopts semi-permeable membranes (permeable watertight)

3. separating tumor cell is made cell suspending liquid

A) absorb the supernatant nutrient solution with suction pipe

B) add colloid enzyme (preparing)

C) the refrigeration test tube is put into test tube flat (regularly measuring alarm).Put into swing constant incubator after starting timing, thermometric, warning device, after hatching half an hour.

D) will refrigerate the test tube connecting tube, be inverted in test-tube stand, clean concentrated repeatedly with the TFF device.

E) tumor cell suspension of making is inserted the refrigeration test tube, puts into the liquid nitrogen vessel prolonged preservation.

F) each patient has a disposal reagent kit, comprises nutrient solution, TFF special container, TFF strainer, the corresponding pipeline of refrigeration test tube, band antibiotic, and same barcode (ID that patient is unique) is arranged on each article.

G) collagenase and damping fluid are prepared in the laboratory.

H) all should under the LIMS system, operate before each operation.

Claims

1. long-term reefer system of tumour cell profound hypothermia is characterized in that: this is a kind ofly to clean concentrating unit, management system, liquid nitrogen vessel, profound hypothermia by test tube flat, cell in profound hypothermia refrigeration test tube, Transport Package box, swing property constant incubator, the incubator and freeze the device that case is formed.

2. by the long-term reefer system of the described tumour cell profound hypothermia of claim 1, it is characterized in that: profound hypothermia refrigeration test tube non-toxic plastic, or metallic substance preparation, and ability profound hypothermia-198 ℃, its upper end band outside nut, cooperate with the test tube lid, test tube lid is used for sealing test tube, the band internal thread, and match with screw in vitro and to be connected, test tube has covered a hole, be used for fixing the pipeline with extraneous traffic, closed and fixed with silicone rubber gasket between test tube lid hole and the pipeline, the test tube lid is made with metal and plastics, the anti-profound hypothermia of palpus, fixing by silicone rubber gasket and test tube lid with the pipeline that the external world has a common boundary, its top has outside screw to link to each other with cap, and available metal or non-toxic plastic are made, cap sealing test tube and extraneous traffic with extraneous traffic pipeline have inside screw to cooperate with pipeline.

3. by the long-term reefer system of the described tumour cell profound hypothermia of claim 1, it is characterized in that: Transport Package are box-packed 37 ℃ of constant temperature displays temperatures and warning device, wherein test tube flat is used for fixing test tube in the incubator, and profile matches with test tube, can stably be fixed in the incubator.

4. by the long-term reefer system of the described tumour cell profound hypothermia of claim 1, it is characterized in that: cell cleans concentrating unit and adopts the ultra-filtration membrane concentrating unit, adopts 0.4 μ m filtering membrane, and the refrigeration test tube is upside down on the test-tube stand, the ultra-filtration membrane concentrate container has side mouth, suitable for reading, end opening, can use repeatedly.

5. by the long-term reefer system of the described tumour cell profound hypothermia of claim 1, it is characterized in that: tumour cell cracking preparation facilities adopts ultrasonic wave tumour antigen preparation facilities and refrigeration test tube corollary apparatus, ultrasonic transducer places the test tube flat bottom, ultrasonic-frequency power supply places the bottom equally, or adopts the frozen-thawed cell cracker to refrigerate the test tube base especially.