CN2776673Y - Preparation of chromatographic column by helical compression - Google Patents
Preparation of chromatographic column by helical compression Download PDFInfo
- Publication number
- CN2776673Y CN2776673Y CN 200520039997 CN200520039997U CN2776673Y CN 2776673 Y CN2776673 Y CN 2776673Y CN 200520039997 CN200520039997 CN 200520039997 CN 200520039997 U CN200520039997 U CN 200520039997U CN 2776673 Y CN2776673 Y CN 2776673Y
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- plate
- cover plate
- helical compression
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- column
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Abstract
The utility model relates to a preparing chromatographic column by helical compression, which is characterized in that the upper end of a column body is connected with a cover plate by screw threads, the lower end of the column body is connected with a bottom plate by screw threads, and an upper sieve plate and a lower sieve plate which have outer diameters slightly smaller than the inner diameter of the column body and can be slid up and down are arranged in the column body; the lower end of an inner column of the cover plate forms a flat conical shape, and an O type sealing ring is arranged between the cover plate and the upper sieve plate. The utility model can compress a filling column body the preparing chromatographic column to a tight state through the screw threads for screwing the cover plate and the bottom plate, and has high column efficiency; the preparing chromatographic column made of polymeric materials has the advantages of easy processing, low cost and good corrosion-resistance, therefore the utility model can carry out chromatographic separation with high speed and high efficiency, and is suitable for chromatographic preparation and separation of medical and chemical products of a biological and a chemical engineering and a medicine preparing fields.
Description
Technical field
The utility model belongs to biochemical industry chromatographic separation technology field, relates to a kind of architecture advances of preparative chromatography post.
Background technology
Chromatographic isolation is the present the highest a kind of method of separative efficiency in the separation means of biological chemical field, uses more and more wider.The preparative chromatography post is the critical component that preparative liquid chromatography separates.The cylinder of existing preparation chromatographic column adopts stainless steel and other metal materials more, and its inwall is used special processing technology such as LASER HEAT TREATMENT and process for treating surface and further handled.Though it has higher separating effect, size is less, and each circulation can only separate gram magnitude or the following compound of gram magnitude, and the price of preparative column is very expensive.As diameter is 1cm, and length is that the preparative column void column of 25cm is offered more than 20,000 yuan.The separate object that the preparative chromatography of biological chemical field is separated is a large biological molecule, in separation process, metals such as stainless steel are the high pressure preparative column of cylinder, can produce strong suction-operated to big molecule, macromolecular space structure is changed and then have influence on macromolecular activity.Be applied in addition that the preparative chromatography of biological chemical field separates flow mutually in often contain acid, the solution of alkali or high salt concentration, these solution more or less can produce corrosion to metal cartridges such as stainless steels, pollute separate object.As to adopt glass be the low pressure preparative column of cylinder, separates though can carry out macromolecular preparation, because the flow velocity of low pressure preparation is lower, resolution ratio is also poor, and the effect of preparation separation is bad.And the long operating time also can influence the active of large biological molecule and then reduce its yield.In addition, the processing technology of glass is had relatively high expectations, and also there are some problems in aspects such as withstand voltage, the sealing of the glass preparation post of domestic production at present.
Summary of the invention
The purpose of this utility model be for provide a kind of easily manufactured, price is low, corrosion-resistant, anti-pollution, preparative chromatography post that efficient high-speed can satisfy the biological chemical field chromatographic isolation.
To achieve these goals, the technical scheme that adopts is: a kind of helical compression preparative chromatography post, comprise cylinder, cover plate, base plate, feed pipe and discharge nozzle, it is characterized in that: the upper end of cylinder is threaded with cover plate, the lower end of cylinder is connected with base thread, center, described cover plate upper end, the feed pipe that is with the stubborn joint of left-hand seat is threaded with cover central hole, center, described base plate lower end, the discharge nozzle that is with the stubborn joint of setting about is threaded with bottom center hole, and it is more smaller than cylinder internal diameter that described cylinder is built-in with external diameter, upper sieve plate and following sieve plate can slide up and down.Column end is flat tapered in the described cover plate, is equipped with O type sealed picture between column end and the upper sieve plate in the cover plate.
Described upper sieve plate and following sieve plate are loose structure, the material stainless steel sintered plate of doing, titanium alloy sintered plate or cellular-plastic panels.Cylinder, cover plate and baseboard material are polytetrafluoroethylene (PTFE), polyvinylidene fluoride, polyethers ether cave, nylon or polyethylene.
Left-hand seat is twisted joint and set about to twist joint material is polyvinylidene fluoride, polyethers ether cave, nylon, polyethylene, stainless steel or titanium alloy.The material of feed pipe and discharge nozzle is withstand voltage polytetrafluoroethylene (PTFE), poly-perfluoro second propylene or nylon.
Good effect of the present utility model is: owing to adopt screw connection structure between cylinder and cover plate and the base plate, chromatographic column can be compressed into compact state, post is imitated high, whole system can be born pressure and be reached 2MPa, reduce or eliminate the sex change of large biological molecule under excess pressure, kept higher flow velocity and resolution ratio.The preparative chromatography post adopts polymeric material, and is easy to process, cost is low, corrosion resistance and good, contamination resistance are strong, and the chromatogram preparation that is suitable for biological chemical field and pharmaceutical field medication chemistry product separates.
Description of drawings
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Fig. 1, preparative chromatography rod structure schematic diagram;
Fig. 2, the A-A cutaway view of Fig. 1.
Among the figure, the 1-feed pipe; The 2-left-hand seat is twisted joint; The 3-cover plate; The 4-cover central hole; 5-O type sealed picture; The 6-upper sieve plate; The 7-cylinder; Sieve plate under the 8-; The 9-base plate; The 10-bottom center hole; 11-sets about to twist joint; The 12-discharge nozzle.
The specific embodiment
A kind of helical compression preparative chromatography post, comprise cylinder 7, cover plate 3, base plate 9, feed pipe 1 and discharge nozzle 12, it is characterized in that: the upper end of cylinder 7 is threaded with cover plate 3, the lower end of described cylinder 7 is threaded with base plate 9, center, described cover plate 3 upper end, the feed pipe 1 that is with the stubborn joint 2 of left-hand seat is threaded with cover central hole 4, center, described base plate 9 lower end, be with the discharge nozzle 12 of set about twisting joint 11 and be threaded with bottom center hole 10, described cylinder 7 is built-in with that external diameter is more smaller than cylinder 7 internal diameters, upper sieve plate 6 and following sieve plate 8 can slide up and down.Column end is flat tapered in the described cover plate 3, is equipped with O type sealed picture 5 between column end and the upper sieve plate 6 in the cover plate 3.
Described upper sieve plate 6 and following sieve plate 8 are loose structure, the material stainless steel sintered plate of doing, titanium alloy sintered plate or cellular-plastic panels.Described cylinder 7, cover plate 3 and base plate 9 materials are polytetrafluoroethylene (PTFE), polyvinylidene fluoride, polyethers ether cave, nylon or polyethylene.
Left-hand seat is twisted joint 2 and is set about to twist joint 11 materials and is polyvinylidene fluoride, polyethers ether cave, nylon, gathers Chengdu ethene, stainless steel or titanium alloy.Described feed pipe 1 and discharge nozzle 12 materials are withstand voltage polytetrafluoroethylene (PTFE), poly-perfluoro second propylene or nylon.
During use, in cylinder 7, between upper sieve plate 6 and the following sieve plate 8, filler is filled to proper height, screw thread screws degree between cylinder 7 and cover plate 3 and the base plate 9 by regulating, and stops after the filler cylinder in the preparative chromatography post is compressed to compact state.When separated large biological molecule is evenly distributed on filler cylinder upper end by upper sieve plate 6 after, feed eluent from feed pipe 1 and carry out wash-out, because the filler cylinder has been compressed into compact state, post is imitated high, polymerizable material is more withstand voltage than glass, can apply certain pressure (2MPa), the flow velocity height, thereby can reach at a high speed, purpose that high efficiency chromatography separates.
Claims (6)
1, a kind of helical compression preparative chromatography post, comprise cylinder (7), cover plate (3), base plate (9), feed pipe (1) and discharge nozzle (12), it is characterized in that: the upper end of cylinder (7) is threaded with cover plate (3), the lower end of described cylinder (7) is threaded with base plate (9), center, described cover plate (3) upper end, the feed pipe (1) that is with the stubborn joint (2) of left-hand seat is threaded with cover central hole (4), described base plate (9) center, lower end, the discharge nozzle (12) that is with the stubborn joint (11) of setting about is threaded with bottom center hole (10), and it is more smaller than cylinder (7) internal diameter that described cylinder (7) is built-in with external diameter, upper sieve plate (6) and following sieve plate (8) can slide up and down.
2, helical compression preparative chromatography post according to claim 1 is characterized in that: the interior column end of described cover plate (3) is flat tapered, is equipped with O type sealed picture (5) between interior column end of cover plate (3) and the upper sieve plate (6).
3, helical compression preparative chromatography post according to claim 1 is characterized in that: described upper sieve plate (6) and following sieve plate (8) are loose structure, and material is the stainless steel sintered plate, titanium alloy sintered plate or cellular-plastic panels.
4, helical compression preparative chromatography post according to claim 1 is characterized in that: described cylinder (7), cover plate (3) and base plate (9) material are polytetrafluoroethylene (PTFE), polyvinylidene fluoride, polyethers ether cave, nylon or polyethylene.
5, helical compression preparative chromatography post according to claim 1 is characterized in that: described left-hand seat is twisted joint (2) and set about to twist joint (11) material is polyvinylidene fluoride, polyethers ether cave, nylon, polyethylene, stainless steel or titanium alloy.
6, helical compression preparative chromatography post according to claim 1 is characterized in that: described feed pipe (1) and discharge nozzle (12) material are withstand voltage polytetrafluoroethylene (PTFE), poly-perfluoro second propylene or nylon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520039997 CN2776673Y (en) | 2005-03-08 | 2005-03-08 | Preparation of chromatographic column by helical compression |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520039997 CN2776673Y (en) | 2005-03-08 | 2005-03-08 | Preparation of chromatographic column by helical compression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2776673Y true CN2776673Y (en) | 2006-05-03 |
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ID=36744059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520039997 Expired - Fee Related CN2776673Y (en) | 2005-03-08 | 2005-03-08 | Preparation of chromatographic column by helical compression |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2776673Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102470286A (en) * | 2009-07-30 | 2012-05-23 | 霍夫曼-拉罗奇有限公司 | Moveable chromatography column separator |
-
2005
- 2005-03-08 CN CN 200520039997 patent/CN2776673Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102470286A (en) * | 2009-07-30 | 2012-05-23 | 霍夫曼-拉罗奇有限公司 | Moveable chromatography column separator |
| CN102470286B (en) * | 2009-07-30 | 2015-02-25 | 霍夫曼-拉罗奇有限公司 | Moveable chromatography column separator |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |