CN2736364Y - Gene chip hybridization instrument - Google Patents
Gene chip hybridization instrument Download PDFInfo
- Publication number
- CN2736364Y CN2736364Y CN 200420049237 CN200420049237U CN2736364Y CN 2736364 Y CN2736364 Y CN 2736364Y CN 200420049237 CN200420049237 CN 200420049237 CN 200420049237 U CN200420049237 U CN 200420049237U CN 2736364 Y CN2736364 Y CN 2736364Y
- Authority
- CN
- China
- Prior art keywords
- gene chip
- carrying platform
- hybridization instrument
- support
- chip hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a gene chip hybridization instrument which comprises a bracket and a bearing platform arranged in the bracket. The gene chip hybridization instrument is characterized in that the bracket is provided with a mechanical mechanism capable of driving the bearing platform to move back and forth in vertical direction; the amplitude of each particle of the bearing platform forms a sine wave curve in the vertical direction along with the time variation; the amplitude increases following the increase of the radius in the radius direction of the bearing platform. The utility model can be widely applied to fields of disease diagnosis, life science foundation research, agricultural and environmental monitoring, food hygiene detection, and judicial identification.
Description
Technical field
The utility model relates to the gene chip hybridization instrument, particularly about a kind of hybridization instrument that is applicable to big dot matrix gene chip (as full genomic gene chip).
Background technology
The microarray gene chip is widely used in biology, medical science and association area as a kind of testing tool fast and efficiently.Gene chip is that multiple nucleic acid is selected system and is fixed on the substrate present many employings of substrate standard slide glass with the row of rule and the arrangement mode of row.Usually, point has the area of gene nucleic acid just to belong to big dot matrix gene chip above 22cm * 22cm on gene chip.The number gene that comprises on the big dot matrix gene chip is a lot, and the gene chip of full genome scale is used for the expression variation of analyzing gene often, and then the vital movement process of overall understanding organism, therefore has important practical value.
The analytic process of gene chip need be finished by means of certain hybridization instrument.Existing hybridization instrument is a lot, as the water-bath based on the static hybridization of application of sample, cover glass mode; Based on automatic hybridization instrument of stream mode etc.
Yet these hybridization instruments exist following weak point when being used for the hybridization of big dot matrix gene chip:
(1) adopt application of sample, cover glass is hybridized, and is after big dot matrix gene chip is loaded on hybridizing box, be placed in the water-bath under the certain temperature static hybridization and spend the night.Often uneven phenomenon can appear in hybridization signal, may be because mobile very poor the causing when static hybridization of the hybridization solution under the cover glass.
(2) adopt and to hybridize, often need more hybridization solution based on the automatic hybridization instrument of stream, the probe molecule of as much like this, concentration will be diluted about about 5 times, causes hybridization signal to weaken.
The innovation and creation content
The purpose of this utility model provides a kind ofly both can effectively improve the hybridization signal homogeneity, did not weaken the gene chip hybridization instrument of hybridization signal again.
A kind of gene chip hybridization instrument, it includes a support and and is arranged on the interior carrying platform of support, is provided with one on the described support and can drives the vertically mechanical mechanism of back and forth movement of described carrying platform; The amplitude that each particle of described carrying platform changes vertically in time is a sine wave curve, and amplitude increases along the increase of carrying platform radial direction with radius.
Described support is made up of support body and the top board that is fixed on the support body top; Described mechanical mechanism is arranged on the top board of support, it includes wheel disc, that a motor, is arranged on motor output end and is located in anchor line (string) axle on the described wheel disc, offer three through holes on the top board of described support, described carrying platform and the corresponding place of described through hole respectively are provided with a tie point; Three messenger wires pass described three through holes respectively, and described messenger wire one end is connected the bottom of described anchor line (string) axle, and the other end is connected on the tie point of the carrying platform corresponding with its described through hole that passes.
In actual applications, the top board of described support is preferably circle, and described three through holes are opened on the edge of top board, and uniformly-spaced arrange with 120 ° interval; Described carrying platform also is circular, and described tie point is arranged on the edge of carrying platform, and uniformly-spaced arranges with 120 ° interval.Described anchor line (string) axle is located on the edge of described wheel disc by a bearing.Described carrying platform is provided with the hybridizing box that holds gene chip.Described carrying platform is provided with the hybridizing box that holds gene chip, the quantity of hybridizing box is 1～20, what hold in the hybridizing box both can be the big dot matrix gene chip that the point sample area surpasses 22cm * 22cm, can be again the gene chip of point sample area less than 22cm * 22cm.
Motor of the present utility model is controlled through a motor control unit, described motor control unit comprises switch power supply, motor driver, impulse regulator, inverter, the external common power of described inverter, described switch power supply connects described inverter, described motor driver and impulse regulator all are connected on the described switch power supply, and the pulse signal of impulse regulator is exported to motor driver, and motor driver drives described motor.
The utility model has the following advantages owing to take above design: 1, owing to kept the hybridization mode under the cover glass, therefore the volume of required hybridization solution seldom.2, the hybridizing box that big dot matrix gene chip is housed is assemblied on the platform with special exercise rule, and this characteristics of motion can effectively promote flowing of hybridization solution under the cover glass, therefore can significantly improve the homogeneity of big dot matrix gene chip hybridization signal.3, this utility model has good versatility, can be used for common water-bath.
The utility model can be applicable to association areas such as medical diagnosis on disease, drug screening, life science fundamental research, agricultural and environmental monitoring, food hygiene detection, judicial expertise.Particularly, can be used for the hybridization of full genomic gene chip, for example: the gene of specifically expressing in the full genomic gene chip detection breast cancer tissue of personnel selection.
Description of drawings
Fig. 1 is the utility model structural representation
Fig. 2 is the skeleton diagram of the utility model control unit
Embodiment
As shown in Figure 1, an embodiment of the present utility model mainly includes a support and and is arranged on the interior carrying platform 2 of support.Support is made up of support body 1 and the top board 11 that is fixed on support body 1 top, is provided with one on the top board 11 and can draws the vertically mechanical mechanism 3 of back and forth movement of described carrying platform; Mechanical mechanism 3 includes wheel disc 32, that a motor 31, is arranged on motor output end and is located in anchor line (string) axle 33 on the described wheel disc 32, and in the present embodiment, anchor line (string) axle 33 is located on the edge of wheel disc 3 by a bearing 34; Top board 11 is circular, offer three through holes 12 on its edge, and three through holes 12 is arranged uniformly-spaced with 120 ° interval, carrying platform 2 also is circular, respectively be provided with a tie point 21 with through hole 12 corresponding edges, promptly tie point also is three, and uniformly-spaced arranges with 120 ° interval; Pass three through holes 12 respectively with three messenger wires 4, an end of messenger wire 4 is connected the bottom of anchor line (string) axle 33, and the other end is connected on the tie point 21 of the corresponding carrying platform of the through hole crossed with its biography 12.
In addition, also be provided with the hybridizing box 22 that holds gene chip on the carrying platform 2, hybridizing box 22 can be selected different models for use, and it can hold the big dot matrix gene chip that the gene nucleic acid area surpasses 22cm * 22cm, also can hold the gene chip of gene nucleic acid area less than 22cm * 22cm; The quantity of hybridizing box 22 generally can be 1～20, is 1 in the present embodiment, when needs are used the hybridizing box of greater amt, only needs a corresponding area that increases carrying platform to get final product.
In the present embodiment, motor 31 is controlled through a motor control unit, as shown in Figure 2, motor control unit comprises switch power supply, motor driver, impulse regulator, inverter, the external common power of inverter, switch power supply connects described inverter, and motor driver and impulse regulator all are connected on the switch power supply, and the pulse signal of impulse regulator is exported to motor driver, motor driver drive-motor 31.
In the present embodiment, the shape of top board and carrying platform can be diversified, can be circular, square, Polygons etc., and the quantity of messenger wire and distributing also can change as the case may be simultaneously.
The utility model can directly be placed in the common water-bath when in use and work, and by regulating motor control unit, can open motor, and changes the velocity of rotation of motor; The rotating band runner disc of motor rotates, the rotation of wheel disc drives the anchor line (string) axle and rotates around the axle center of motor, the bottom of anchor line (string) axle is connecting three messenger wires and is also moving along with moving of anchor line (string) axle, because the length of three messenger wires is the same, therefore, the carrying platform that the messenger wire the other end connects is back and forth movement vertically then, and changes in time, each particle of carrying platform amplitude vertically is a sine wave curve, and amplitude increases along the increase of carrying platform radial direction with radius.According to the different needs of different occasions, only need to regulate motor control unit and change rotating speed of motor, just can change the speed of carrying platform up-down vibration, to reach needed requirement.
By facts have proved, the utility model can significantly improve the homogeneity of big dot matrix gene chip hybridization signal, this is because when hybridization, the hybridizing box that holds big dot matrix gene chip is installed on the platform with special exercise rule, and this characteristics of motion can effectively promote flowing of hybridization solution under the cover glass.
Claims (8)
1, a kind of gene chip hybridization instrument, it includes a support and and is arranged on the interior carrying platform of support, it is characterized in that: be provided with one on the described support and can drive the vertically mechanical mechanism of back and forth movement of described carrying platform; The amplitude that each particle of described carrying platform changes vertically in time is a sine wave curve, and amplitude increases along the increase of carrying platform radial direction with radius.
2, a kind of gene chip hybridization instrument according to claim 1 is characterized in that: described support is made up of support body and the top board that is fixed on the support body top; Described mechanical mechanism is arranged on the top board of support, it includes wheel disc, that a motor, is arranged on motor output end and is located in anchor line (string) axle on the described wheel disc, offer three through holes on the top board of described support, described carrying platform and the corresponding place of described through hole respectively are provided with a tie point; Three messenger wires pass described three through holes respectively, and described messenger wire one end is connected the bottom of described anchor line (string) axle, and the other end is connected on the tie point of the carrying platform corresponding with its described through hole that passes.
3, a kind of gene chip hybridization instrument according to claim 2 is characterized in that: the top board of described support is for circular, and described three through holes are opened on the edge of top board, and uniformly-spaced to arrange at 120 ° interval; Described carrying platform also is circular, and described tie point is arranged on the edge of carrying platform, and uniformly-spaced arranges with 120 ° interval.
4, according to claim 2 or 3 described a kind of gene chip hybridization instrument, it is characterized in that: described anchor line (string) axle is located on the edge of described wheel disc by a bearing.
5, according to claim 1 or 2 or 3 described a kind of gene chip hybridization instrument, it is characterized in that: described carrying platform is provided with the hybridizing box that holds gene chip.
6, a kind of gene chip hybridization instrument according to claim 5 is characterized in that: what hold in the described hybridizing box is the big dot matrix gene chip that the point sample area surpasses 22cm * 22cm, and the quantity of described hybridizing box is 1～20.
7, a kind of gene chip hybridization instrument according to claim 5 is characterized in that: what hold in the described hybridizing box is the gene chip of point sample area less than 22cm * 22cm, and the quantity of described hybridizing box is 1～20.
8, according to claim 2 or 3 or 6 or 7 described a kind of gene chip hybridization instrument, it is characterized in that: described motor is controlled through a motor control unit, described motor control unit comprises switch power supply, motor driver, impulse regulator, inverter, the external common power of described inverter, described switch power supply connects described inverter, described motor driver and impulse regulator all are connected on the described switch power supply, and the pulse signal of impulse regulator is exported to motor driver, and motor driver drives described motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420049237 CN2736364Y (en) | 2004-04-19 | 2004-04-19 | Gene chip hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420049237 CN2736364Y (en) | 2004-04-19 | 2004-04-19 | Gene chip hybridization instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2736364Y true CN2736364Y (en) | 2005-10-26 |
Family
ID=35307100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420049237 Expired - Lifetime CN2736364Y (en) | 2004-04-19 | 2004-04-19 | Gene chip hybridization instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2736364Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328505B (en) * | 2007-06-19 | 2011-06-08 | 中华人民共和国北京出入境检验检疫局 | Gene chip and reagent box for detecting food-borne virus |
| CN102533525A (en) * | 2011-12-22 | 2012-07-04 | 亚能生物技术（深圳）有限公司 | Full-automatic hybridization appliance |
-
2004
- 2004-04-19 CN CN 200420049237 patent/CN2736364Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328505B (en) * | 2007-06-19 | 2011-06-08 | 中华人民共和国北京出入境检验检疫局 | Gene chip and reagent box for detecting food-borne virus |
| CN102533525A (en) * | 2011-12-22 | 2012-07-04 | 亚能生物技术（深圳）有限公司 | Full-automatic hybridization appliance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Qin et al. | Rational design and synthesis of catalytically driven nanorotors | |
| Charles | Technical aspects of the rheological properties of microbial cultures | |
| Woodley et al. | The use of windows of operation as a bioprocess design tool | |
| CN101298592B (en) | Cell three-dimensional mechanical loading unit | |
| CN2736364Y (en) | Gene chip hybridization instrument | |
| Zou et al. | Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m 3 fermenter | |
| CN102533525A (en) | Full-automatic hybridization appliance | |
| CN103487425A (en) | Method for distinguishing cancer cells through surface enhanced Raman spectroscopy | |
| Tramper et al. | Scale-up considerations and bioreactor development for animal cell cultivation | |
| Lins Barros et al. | Gas dispersion in non-Newtonian fluids with mechanically agitated systems: A review | |
| De Lora et al. | Centrifugal generation of droplet-based 3D cell cultures | |
| Trivedi et al. | Open source 3-D printed nutating mixer | |
| CN1959351A (en) | Embedded type detecting instrument for agricultural plant growth environment, and working method | |
| Keßler et al. | The effect of the aggregate size on the production of ajmalicine and tryptamine in Catharanthus roseus suspension culture | |
| WEAVER | Growth of grapes in relation to gibberellin | |
| Casal et al. | Nanorobots as cellular assistants in inflammatory responses | |
| Yedgar et al. | RBC adhesion to vascular endothelial cells: more potent than RBC aggregation in inducing circulatory disorders | |
| Kaupp et al. | Scalar Relativistic Effects on 17O NMR Chemical Shifts in Transition-Metal Oxo Complexes. An ab Initio ECP/DFT Study.[Erratum to document cited in CA122: 254385] | |
| CN207832623U (en) | A kind of apparatus for measuring concentration | |
| CN214529050U (en) | High-flux miniature parallel biological reaction device | |
| CN1148457C (en) | Nanometer particle mark gene probe and its preparing method and use | |
| CN100335655C (en) | Oligonucleotide micro-array chip for detecting small molecule RNA | |
| CN207576453U (en) | Detect the base station device of determinand | |
| AL Thagafi et al. | Revolutionizing Healthcare: The Technological Transformation of Medical Laboratory Outcomes | |
| CN2485313Y (en) | Multifunctional molecular hybridizationer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: BOAO BIOLOGICAL CO., LTD.; PATENTEE Free format text: FORMER NAME OR ADDRESS: CAPITALBIO CORPORATION; PATENTEE |
|
| CP03 | Change of name, title or address |
Address after: 102206 No. 18, life science Road, Beijing, Changping District Co-patentee after: Tsinghua University Patentee after: Capitalbio Corporation Address before: 102206 No. 18, life science Road, Beijing, Changping District Co-patentee before: Tsinghua University Patentee before: Boao Biochip Co., Ltd., Beijing |
|
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |
Expiration termination date: 20140419 Granted publication date: 20051026 |