CN214529050U - High-flux miniature parallel biological reaction device - Google Patents
High-flux miniature parallel biological reaction device Download PDFInfo
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- CN214529050U CN214529050U CN202120688765.4U CN202120688765U CN214529050U CN 214529050 U CN214529050 U CN 214529050U CN 202120688765 U CN202120688765 U CN 202120688765U CN 214529050 U CN214529050 U CN 214529050U
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Abstract
The utility model discloses a high-flux micro parallel bioreactor, which comprises at least one micro bioreactor, wherein the micro bioreactor comprises a stator winding driving device, rotor permanent magnet steel, a reactor head cover plate, a stirring component, a bioreactor tank body, a sampling pipe, a bioreactor tank bottom, an air inlet pipe and a temperature control device; the winding driving device is arranged on the upper part of the tank body of the bioreactor, and the temperature control device is connected to the bottom of the tank body of the bioreactor. Compared with the prior art, the utility model the advantage be: the utility model provides a limited problem of too big operation space of bioreactor volume, it is little to have the occupation of land space, and convenient operation's advantage can realize a plurality of bioreactors's coordinated control simultaneously, satisfies the bacterial screening of high flux and the application of technology and formula optimization experiment.
Description
Technical Field
The utility model relates to a biological reaction device, in particular to a high-flux miniature parallel biological reaction device.
Background
Bioreactors are the primary driver to advance the bioproduct industry. Before any biological product is industrialized, the continuous amplification processes of strain screening, small test, pilot test, large production and the like are required. The screening of microbial strains, the optimization of culture media and the optimization of processes are all independent of a bioreactor for experiments, and are used for simulating various process parameters and environmental conditions on an industrial bioreactor. The conventional experimental bioreactor and the production bioreactor are consistent in structure, stirring form, online parameter detection principle and method, and can well simulate measurement and control modes and environmental conditions in industrialization. However, when a conventional bioreactor is generally used for high-throughput screening, the data generation speed is far from meeting the requirements of new technologies such as machine learning, big data mining and the like due to the influences of a large volume, a large occupied space, limited reactor equipment quantity, large culture medium consumption and the like. Therefore, the conventional bioreactor is not suitable for screening and researching high-flux strains, culture media and processes.
The micro bioreactor has small volume and small occupied space, can synchronously carry out a large amount of experimental screening and optimization, and has an operation mode similar to that of the amplified bioreactor, so the amplification of experimental data is obviously superior to that of a shake flask and micropore culture, and the micro bioreactor is more and more favored by researchers in high-throughput screening. However, due to the limitation of the operation space, the requirements on the equipment configuration and the online parameter detection method and the process regulation and control mode are more strict.
Therefore, the development of a high-throughput micro parallel bioreactor is a problem to be solved by those skilled in the art.
Disclosure of Invention
The utility model provides a compact structure, convenient operation and high flux micro parallel bioreactor to solve the above disadvantages.
The above object of the present invention is achieved by the following technical solutions: a high-flux miniature parallel bioreactor comprises at least one miniature bioreactor, and a control system can simultaneously control thousands of miniature bioreactors; the micro bioreactor comprises a stator winding driving device, rotor permanent magnet steel, a reactor end cover plate, a stirring assembly, a bioreactor tank body, a sampling pipe, a bioreactor tank bottom, an air inlet pipe and a temperature control device; the winding driving device is arranged on the upper part of the tank body of the bioreactor, and the temperature control device is connected to the bottom of the tank body of the bioreactor.
Furthermore, the stator winding driving device is formed by winding a winding coil, a magnetic field which rotates around the geometric axis of the motor in a full rotation mode is formed around the winding coil by controlling alternating frequency waveforms of current waves on the winding coil, and the magnetic field can drive the rotor permanent magnet steel on the rotor to rotate.
Further, the stirring assembly comprises rotor permanent magnet steel, a stirring rotating shaft and a stirring paddle, the rotor permanent magnet steel is connected with the stirring rotating shaft, and the stirring paddle is fixed on the lower portion of the stirring rotating shaft. The stirring performance can be changed by adjusting the number of the magnetic steels and the magnetic flux intensity of the magnetic steels.
Furthermore, a magnetic steel top fixing hole, an air outlet hole, a sampling hole, an air inlet hole and a material supplementing hole are formed in the reactor head cover plate, and the reactor head cover plate is provided with internal threads and is connected with external threads arranged at the upper end of the bioreactor tank body.
Further, the bioreactor tank is a cylindrical structure, but is not limited to the cylindrical structure, and the bioreactor tank can be made of disposable polystyrene or reusable borosilicate glass and stainless steel, and the volume of the bioreactor tank is 10-100 ml.
Further, install non-invasive optical sensor subassembly and (mixing) shaft fixing device on the bioreactor tank bottom, non-invasive optical sensor includes pH sensor, Do sensor, OD sensor and temperature sensor.
Furthermore, the temperature control device is controlled by a semiconductor, and specifically, a Peltier semiconductor element is embedded into the base of the micro bioreactor to perform heating and refrigerating operations.
Further, the stirring assembly device is arranged in the bioreactor tank body, the upper end of the stirring assembly device is connected to the bottom of the rotor permanent magnet steel, the upper end of the rotor permanent magnet steel is installed in a magnetic steel top fixing hole in the cover plate of the reactor head, and the lower end of the stirring assembly device is installed in the stirring shaft fixing device at the bottom of the bioreactor tank.
During operation, the stator winding driving device can drive the rotor permanent magnet steel to rotate, and the rotor permanent magnet steel drives the stirring rotating shaft and the stirring paddle to rotate, so that the mixing operation is completed. The non-contact optical sensor embedded in the bottom of the reactor can complete the measurement of corresponding on-line parameters, and the semiconductor temperature control device at the bottom can complete the temperature control of the reactor.
Compared with the prior art, the utility model the advantage be: the device adopts the winding drive magnet steel to control the stirring subassembly, and characteristic indexes such as rotational speed direction, drive strength can all independently high-efficiently control. The reactor is internally provided with a non-invasive optical sensor, and the problem of limited operation space caused by overlarge volume of the bioreactor is solved in the process of realizing process parameter monitoring. The utility model discloses a miniature parallel bioreactor of high flux has that occupation of land space is little, and convenient operation's advantage can realize a plurality of bioreactors's coordinated control simultaneously, satisfies the application of high flux bacterial screening and technology and formula optimization experiment.
Drawings
FIG. 1 is a schematic diagram of a high throughput parallel bioreactor configuration.
FIG. 2 is a schematic view of the structure of the opening of the cover plate of the bioreactor closure.
Fig. 3 is a schematic view of the stirring assembly.
FIG. 4 is a schematic view of the bottom structure of the bioreactor tank.
Fig. 5 is a schematic diagram of an 8-winding structure.
Fig. 6 is a schematic structural diagram of an 8-joint temperature control device.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
As shown in FIGS. 1 to 6, the high-throughput mini-parallel bioreactor in this embodiment is a set of 8-linked devices, each mini-bioreactor can be controlled independently or simultaneously in parallel, the high-throughput mini-parallel bioreactor comprises at least one mini-bioreactor, and the control system can control thousands of mini-bioreactors simultaneously; the micro bioreactor comprises a stator winding driving device 1, rotor permanent magnet steel 2, a reactor end socket cover plate 3, a stirring assembly 4, a bioreactor tank body 5, a sampling pipe 6, a bioreactor tank bottom 7, an air inlet pipe 8 and a temperature control device 9; the winding driving device 1 is arranged at the upper part of the bioreactor tank body 5, and the temperature control device 9 is connected to the bottom of the bioreactor tank body 5. In the utility model, each stator winding driving device 1 can independently control a micro bioreactor.
Furthermore, the stator winding driving device 1 is formed by winding a winding coil, and a magnetic field which rotates around the geometric axis of the motor in a full rotation mode is formed around the winding coil by controlling the alternating frequency waveform of current waves on the winding coil, and the magnetic field can drive the rotor permanent magnet steel 2 on the rotor to rotate.
Further, stirring subassembly 4 includes rotor permanent magnetism magnet steel 2, stirring pivot 4.1 and stirring rake 4.2, rotor permanent magnetism magnet steel 2 connects stirring pivot 4.1, stirring rake 4.2 is fixed in stirring pivot 4.1 lower part. The stirring performance can be changed by adjusting the number of the magnetic steels and the magnetic flux intensity of the magnetic steels.
Further, a magnetic steel top fixing hole 3.1, an air outlet hole 3.2, a sampling hole 3.3, an air inlet hole 3.4 and a material supplementing hole 3.5 are formed in the reactor head cover plate 3, and the reactor head cover plate 3 is provided with internal threads and is connected with external threads arranged at the upper end of the bioreactor tank body 5.
Further, the bioreactor tank 5 is a cylindrical structure, but is not limited to this structure, and the material thereof may be disposable polystyrene material or reusable borosilicate glass and stainless steel material, and the volume is 10-100 ml.
Further, a non-invasive optical sensor assembly and a stirring shaft fixing device 7.5 are mounted on the bioreactor tank bottom 7, and the non-invasive optical sensors comprise a pH sensor 7.1, a Do sensor 7.2, an OD sensor 7.3 and a temperature sensor 7.4.
Further, the temperature control device 9 is controlled by a semiconductor, specifically, a Peltier semiconductor element is embedded in the base of the micro bioreactor to perform heating and cooling operations.
Further, the stirring assembly device 4 is arranged in the bioreactor tank body 5, the upper end of the stirring assembly device is connected to the bottom of the rotor permanent magnet steel 2, the upper end of the rotor permanent magnet steel 2 is arranged in a magnet steel top fixing hole 3.1 on the reactor head cover plate 3, and the lower end of the rotor permanent magnet steel is arranged in a stirring shaft fixing device 7.5 at the bottom 7 of the bioreactor tank.
During operation, the stator winding driving device 1 can drive the rotor permanent magnet steel 2 to rotate, and the rotor permanent magnet steel 2 drives the stirring rotating shaft 4.1 and the stirring paddle 4.2 to rotate, so that the mixing operation is completed. The non-contact optical sensor embedded in the bottom of the reactor can complete the measurement of corresponding on-line parameters, and the semiconductor temperature control device at the bottom can complete the temperature control of the reactor.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.
Claims (10)
1. A high-flux miniature parallel biological reaction device is characterized in that: the device comprises at least one micro bioreactor, wherein the micro bioreactor comprises a stator winding driving device, rotor permanent magnet steel, a reactor end socket cover plate, a stirring assembly, a bioreactor tank body, a sampling pipe, a bioreactor tank bottom, an air inlet pipe and a temperature control device; the winding driving device is arranged on the upper part of the tank body of the bioreactor, and the temperature control device is connected to the bottom of the tank body of the bioreactor.
2. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the stator winding driving device is formed by winding a winding coil, a magnetic field which rotates around the geometric axis of the motor completely is formed around the winding coil by controlling the alternating frequency waveform of current waves on the coil, and the magnetic field can drive the rotor permanent magnetic steel on the rotor to rotate.
3. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the stirring assembly comprises rotor permanent magnet steel, a stirring rotating shaft and a stirring paddle, the rotor permanent magnet steel is connected with the stirring rotating shaft, and the stirring paddle is fixed on the lower portion of the stirring rotating shaft.
4. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the reactor head cover plate is provided with a magnetic steel top fixing hole, an air outlet hole, a sampling hole, an air inlet hole and a material supplementing hole, and is provided with internal threads connected with external threads arranged at the upper end of the bioreactor tank body.
5. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the bioreactor tank body is of a cylindrical structure.
6. A high throughput micro-scale parallel biological reaction device according to claim 5, wherein: the bioreactor tank body is made of disposable polystyrene or reusable borosilicate glass and stainless steel.
7. A high throughput micro-scale parallel biological reaction device according to claim 5 or 6, wherein: the volume of the bioreactor tank body is 10-100 ml.
8. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: install non-invasive optical sensor subassembly and (mixing) shaft fixing device on the bioreactor tank bottoms, non-invasive optical sensor includes pH sensor, Do sensor, OD sensor and temperature sensor.
9. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the temperature control device is controlled by a semiconductor, and specifically, a Peltier semiconductor element is embedded into a base of the micro bioreactor to perform heating and refrigerating operations.
10. A high throughput micro-scale parallel biological reaction device according to claim 1, wherein: the stirring assembly device is arranged in the bioreactor tank body, the upper end of the stirring assembly device is connected to the bottom of rotor permanent magnet steel, the upper end of the rotor permanent magnet steel is arranged in a magnetic steel top fixing hole in a reactor head cover plate, and the lower end of the stirring assembly device is arranged in a stirring shaft fixing device at the bottom of the bioreactor tank.
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| CN202120688765.4U CN214529050U (en) | 2021-04-06 | 2021-04-06 | High-flux miniature parallel biological reaction device |
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| CN202120688765.4U CN214529050U (en) | 2021-04-06 | 2021-04-06 | High-flux miniature parallel biological reaction device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114814157A (en) * | 2022-06-24 | 2022-07-29 | 中国煤炭地质总局勘查研究总院 | Coal bed gas bio-enrichment experiment system |
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2021
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114814157A (en) * | 2022-06-24 | 2022-07-29 | 中国煤炭地质总局勘查研究总院 | Coal bed gas bio-enrichment experiment system |
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