CN2485313Y

CN2485313Y - Multifunctional molecular hybridizationer

Info

Publication number: CN2485313Y
Application number: CN 01228906
Authority: CN
Inventors: 张家洵; 余力; 阎征; 顾力为; 刘芬峦; 王申五
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-06-21
Filing date: 2001-06-21
Publication date: 2002-04-10
Anticipated expiration: 2011-06-21

Abstract

The utility model belongs to a gene analysis apparatus which comprises a constant temperature platform that can heat up and refrigerate as well as a hybridization vessel framework which can move fro and back. The reaction temperature and time and the velocity of the relative movement between the reaction fluid and reflection object is controlled by a micro computer, thereby realizing the automatic hybridization reflection between a gene membrane and a chip and a nucleic acid molecule at a micropore board(strip). The utility model resolves the problems of incapability of low temperature conservation to offspring, low efficiency and easy pollution caused by too much dosage of the reflection fluid.

Description

The polyfunctional molecule hybridization instrument

The utility model is a kind of field of molecular biotechnology that can be applicable to, and has the molecular hybridization reaction kit of multiple function, belongs to the genetic analysis instrument class.

The probe of fixed nucleic acid energy capture of labels on microporous membrane, slide, chip and the microwell plate of synthetic, thereby combine with the amplified production of testing gene, can be used to detect specific biological intravital gene expression dose, the gene expression difference of more different organisms, and polymorphism and sudden change that can analyzing gene, even can carry out the order-checking of DNA.Its result can be used for diagnosing various communicable diseases, heredopathia and tumour and can carry out the screening of active drug.

The reaction of present molecular hybridization is carried out usually in the following way: (1) has the nylon membrane of genetic stew to be sealed in the common plastics bag point to be placed in the water bath with thermostatic control again; (2) have the nylon membrane of genetic stew to be sealed in Glass tubing point after, be placed in the thermostat container and move in a circle.The hybridization that in the common plastics bag, carries out, often the breakage because of plastics bag causes reaction liquid to leak and pollution; The hybridization that carries out in the Glass tubing often because Glass tubing and diaphragm-operated geometrical dimension are difficult to perfect match, will be put into more reaction solution sometimes experimentation cost is strengthened.The water bath with thermostatic control and the thermostat container that are used for molecular hybridization now all do not have refrigerating function, so can't carry out cryopreservation to reaction product.

The purpose of the utility model is to control various temperature and hold-time required in the hybridization process for the user provides a kind of automatically by minicomputer, and can be as required in hybridizing box or microwell plate (bar) go up and chip on carry out multiple mode hybridization and can be to the self-reacting device of reaction product cryopreservation.

But the utility model is achieved in that temperature platform, the hybridization container frame that can move reciprocatingly and minicomputer three parts that alternating temperature is set in instrument.The temperature of temperature platform and constant temperature time are to be realized by refrigerating assembly heating of interface circuit control semi-conductor electricity or refrigerating working order by minicomputer.Constant temp and corresponding work time are shown by digital indicator.Hybridization can will be placed with the hybridizing box of gene diaphragm or chip respectively as required, and the microwell plate (bar) of bag quilt is placed on the hybridization container frame of temperature platform and can be provided with as required whether framework moves reciprocatingly so that reaction solution can fully contact with sample and react.Adopt the not only carrier of enabling hybridization reaction thing (diaphragm, chip and the microwell plate etc.) variation but also make temperature of reaction and the time length accurately and promptly realizes under minicomputer control of this instrument, thereby, target molecule and probe are fully combined to guarantee the specificity of reaction result.Adopt this instrument, not only realized the automatization of hybridization but also can reduce the usage quantity of various reaction solutions and can preserve reaction product at low temperatures.

It is as follows that structure of the present utility model and working condition reach description of drawings in conjunction with the embodiments:

Fig. 1 is a sectional view of the present utility model;

Fig. 2 is a functional block diagram of the present utility model;

Fig. 3 is the programflow chart of minicomputer of the present utility model.

An embodiment called after 1002 type polyfunctional molecule hybridization instruments of the present utility model.It comprises temperature platform [1], and heating-refrigeration unit [2] moves back and forth unit [3], minicomputer [4] and cabinet [5].

1. temperature platform [1] closely touches and peripheral hardware stay-warm case [6] with thermal grease conduction with heating-refrigeration unit [2].What heating-refrigeration unit [2] adopted is that two groups of semi-conductor electricity cooling assemblies [7] are to heat temperature platform [1] or to freeze.Divide into radiator element [8] and axial fan [9] at semi-conductor electricity cooling assembly [7].RTD type temperature sensor [10] is set in temperature platform [1], it is the measuring element of temperature platform [1] temperature, it detects temperature platform [1] temperature signal and again semi-conductor electricity refrigerating assembly [7] is carried out closed-loop control after temperature control interface circuit [11] feeds back to minicomputer [4], to realize the required steady temperature of temperature platform [1].Temperature platform [1] working temperature and constant time can be from the keyboard inputs of the control panel of minicomputer [4].The instantaneous value of temperature and time and the step that is entered can be shown by indicating meter [12].Test each is alarmed by hummer [13] when finishing in stage.

2. the to-and-fro movement unit [3] that drives hybridization container frame [14] is that the output shaft [16] by ND-D type motor [15] passes motion to the steamboat [17] on this, an eccentric pin shaft [18] is housed on steamboat [17], motion is passed to connecting rod [19] and is driven hybridization container frame [14] by bearing pin [18] and does and the parallel to-and-fro movement of temperature platform [1] along guide rail [20], and the speed of its motion is controlled by motion interface circuit [21] by the keyboard input of the control panel of minicomputer [4].

Claims

1. polyfunctional molecule hybridization instrument is characterized in that:

It comprises a temperature platform [1] that has heating-refrigeration unit [2] and temperature sensor [10];

A hybridization container frame [14] that is driven by to-and-fro movement unit [3] places temperature platform [1] top;

One is carried out closed-loop control by temperature interface circuit [11] to the working order (steady temperature and working hour) of heating-refrigeration unit [2] by minicomputer [4], by motion interface circuit [21] speed and the working hour of to-and-fro movement unit [3] are controlled again, the instantaneous value of temperature and time in each stage of experiment all can be shown by digital indicator [12], is reported to the police by hummer [13] when experiment is finished.

2. it is characterized in that according to the polyfunctional molecule hybridization instrument in claims 1 heating-the refrigeration unit [2] of temperature platform is made up of semi-conductor electricity refrigerating assembly [7], scatterer [8] and fan [9].

3. the execution unit that it is characterized in that driving the to-and-fro movement unit [3] of the hybridization container frame [14] on the temperature platform [1] according to the polyfunctional molecule hybridization instrument in claims 1 is motor [15], output shaft [16], has steamboat [17], connecting rod [19] and a guide rail [20] of eccentric pin shaft [18].

4. it is characterized in that according to the polyfunctional molecule hybridization instrument in claims 1 temperature platform [1] is provided with stay-warm case [6].