CN2658746Y - Electrophoresis mould set having vertical carrier piece electrophoretic groove - Google Patents

Electrophoresis mould set having vertical carrier piece electrophoretic groove Download PDF

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Publication number
CN2658746Y
CN2658746Y CN 200320104301 CN200320104301U CN2658746Y CN 2658746 Y CN2658746 Y CN 2658746Y CN 200320104301 CN200320104301 CN 200320104301 CN 200320104301 U CN200320104301 U CN 200320104301U CN 2658746 Y CN2658746 Y CN 2658746Y
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CN
China
Prior art keywords
slide glass
electrophoresis
casket seat
casket
vertical type
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CN 200320104301
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Chinese (zh)
Inventor
陈慧婉
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Weide Biotechnology Co ltd
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Weide Biotechnology Co ltd
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Priority to CN 200320104301 priority Critical patent/CN2658746Y/en
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Abstract

The utility model relates to an electrophoresis module for a vertical electrophoresis tank with glass slides, to a biological detection accessory device which secures the glass slides simply, simplifies the electrophoresis separation project, effectively prevent the glass slides from moving, enhances the accuracy for the electrophoresis separation project and stops the damage to the glass slides, and consists of a vertical box seat, two groups of securing modules equipped at right and left sides of the box seat. At least one side of the box seat is provided with an upwards-opened concave portion. A buffering component is arranged around the concave portion. Convex poles are arranged at left and right sides of the box seat. The securing modules are composed of securing body and knobs driving the securing body. The securing body is provided with a plurality of guide grooves and obliquely-pushed grooves on the convex poles in a parallel securing direction of the glass slides at locations corresponding to the box seat. The knob is provided with two handspikes respectively extended into and corresponded to the obliquely-pushed grooves on the front and rear securing bodies.

Description

The electrophoresis module of vertical type slide glass electrophoresis tank
Technical field
The utility model belongs to the biological detection auxiliary equipment, particularly a kind of electrophoresis module of vertical type slide glass electrophoresis tank.
Background technology
Electrophoresis is at the separation of DNA in the biological substance (deoxyribose nucleic acid), RNA (ribonucleic) and PROTEIN (protein) and analyzes designed, its principle is to utilize the current drives molecule, molecule is vacillated on porous material gel (gel), borrow when mobile its ionic composition the difference in size of electrically charged and molecule itself, and with separated from one another, in the application of biotechnology basic theory, accounted for a very important ring.
As shown in Figure 1, before carrying out the electrophoresis separation engineering, need earlier with desire to separate and the sample solution analyzed and the space of gel injection slide glass group 30.Slide glass group 30 is for being provided with the pad 32 of suitable thickness around the thin slice of making in two sheet glass or acryl material 31, after using anchor clamps 60 with two plate sheets, 31 clippings again, intercept to make between two plate sheets 31 by pad 32 thickness and form the space, then sample solution and gel are injected in the space of slide glass group 30, after treating the gel coagulation forming, the gel-filled slide glass group 30 of envelope can be inserted in the electrophoresis tank, be carried out the electrophoresis separation engineering.
The anchor clamps 60 of the clipping slide glass group of commonly using 30 only have clamping action, and can't slide glass group 30 effectively be located, move in the process of electrophoresis tank thereby make two plate sheets 31 solidify the back, easily because 31 pinches of two plate sheets move or whole group slide glass group 30 collisions and influence gel making effect at the gel-filled or gel of envelope.Especially, whole slide glass group 30 more need carefully be placed on the bolster to wait for the gel sets moulding, makes the bulk sample production process be tending towards complicated, and causes equipment or sample to damage easily.
Therefore, can't as shown in Figure 2, utilize framework 70 directly to push two plate sheets 31 really with the shortcoming of slide glass group 30 location by adjusting screw 71 for improving general clamp, and with slide glass group 30 location, to avoid collision.Yet, because each group slide glass group 30 must be by the adjustment screw more than four 71 by press fit, respectively adjust the degree of depth that screw 71 is pushed to such an extent as to can't quick and precisely adjust, make the gap between two plate sheets 31 can't be average, especially adjusting under the rotation of screw 71 exertins, also may cause the damage of two plate sheets 31.
In addition, the electrophoretic separation device of commonly using is mainly separation, the analysis of being carried out molecule by electrophoresis tank, and the clamp structure of aforesaid two kinds of slide glass groups, its slide glass group is after finishing sample making, still need to move on to again in the electrophoresis tank, via complicated fixedly action the slide glass group is located again, so still be not so good as the facility of expection in the use by the bolster taking-up, even can because of complicated action error influence molecular linkage map accuracy
Summary of the invention
The purpose of this utility model provide a kind of make slide glass group clipping fixing simple, simplify the electrophoretic separation engineering, effectively prevent that the slide glass group from scooping up the accuracy of moving, improve the electrophoretic separation engineering, stopping to damage the electrophoresis module of the vertical type slide glass electrophoresis tank of slide glass group.
The utility model comprises vertical casket seat and is located at two groups of clipping assemblies at casket, right two ends; The side of at least one side of casket seat is provided with opening recess up, is provided with bolster in the recess periphery; Casket, right-hand member are respectively equipped with projection; The clipping assembly comprises at least one clamp and the knob that moves in order to the drive clamp; Clamp be provided with some correspondences and be sheathed on the casket seat relative position place projection and with parallel guide channel and the oblique pushing goove of slide glass group clipping direction; Knob be provided with two corresponding respectively and stretch into the push rod in the oblique pushing goove on forward and backward two clamps.
Wherein:
The clipping assembly comprises two with respect to the opposite forward and backward clamp of the forward and backward side structure equidirectional of casket seat.
The side of casket seat both sides is provided with opening recess up, is provided with bolster in the recess periphery.
Both sides, casket seat top are respectively equipped with the electrode contact that is connected with power lead, and are provided with the lead that extends to casket seat bottom between two electrode contacts.
The casket seat is inner to be provided with the passage that unrolls, and in the passage two ends of unrolling the pipe adapter for inlet pipeline and outlet pipeline linking is set.
The design of casket seat recess bottom surface is the effect of rising with the acceleration bubble is gradually hanged down in both sides towards the center configuration.
Can set support body outside casket seat and two groups of clipping assemblies; Support body has the base plate of placing for the casket seat, upright on the base plate wall that encloses corresponding to casket seat frame is set, and is provided with corresponding to casket seat two side positions on base plate and protrudes out to the lug of casket seat end face, and lug is provided with the axis hole that wears for camshaft; Camshaft is provided with cam corresponding to slide glass group apical margin position, and base plate is provided with elastic pad corresponding to slide glass group root edge position.
The trepanning of planting for camshaft is set on the support body base plate.
Support body wall interior sidewall surface is provided with the raised line in order to guiding casket seat location.
Because the utility model comprises vertical casket seat and is located at two groups of clipping assemblies at casket, right two ends; The side of at least one side of casket seat is provided with opening recess up, is provided with bolster in the recess periphery; Casket, right-hand member are respectively equipped with projection; The clipping assembly comprises at least one clamp and the knob that moves in order to the drive clamp; Clamp be provided with some correspondences and be sheathed on the casket seat relative position place projection and with parallel guide channel and the oblique pushing goove of slide glass group clipping direction; Knob be provided with two corresponding respectively and stretch into the push rod in the oblique pushing goove on forward and backward two clamps.During use, to organize the slide glass group is positioned between casket seat recess and the left and right two ends clipping assembly clamp, cooperate the knob that rotates left and right two ends again, by stretching into push rod in the clamp oblique pushing goove 24 at the oblique pushing goove intrinsic displacement, to promote the clamp displacement, simultaneously, by the guiding that is sheathed on guide channel on the casket seat projection, make the clamp synchronous shift, and the slide glass group pushed to recess and bolster direction, make the slide glass group by closely by press fit, and be able to slide glass group clipping be fixed, and prevent effectively that the slide glass group from scooping up and move via simple rotation knob action.When clamp is pushed the slide glass group, prevent by bolster that the slide glass group from scooping up and move or too greatly slide glass group 30 is broken because of acting force.When desire was taken out the slide glass group, rotary knob can make clamp unclamp in the opposite direction.Not only make slide glass group clipping fixing simple, simplify the electrophoretic separation engineering, and prevent that effectively the slide glass group from scooping up the accuracy of moving, improve the electrophoretic separation engineering, stopping to damage the slide glass group, thereby reach the purpose of this utility model.
Description of drawings
Fig. 1, slide glass group structural representation stereographic map (fixing) with anchor clamps for commonly using.
Fig. 2, slide glass group structural representation stereographic map (fixing two thin slices) in screw locking mode for commonly using.
Fig. 3, be the utility model decomposition texture schematic isometric.
Fig. 4, be the utility model user mode synoptic diagram (install slide glass group state).
Fig. 5, be the utility model user mode synoptic diagram (clipping slide glass group state).
Fig. 6, be the utility model user mode synoptic diagram (place electrophoresis tank in).
Fig. 7, be casket holder structure schematic isometric in the utility model.
Fig. 8, be A-A cut-open view among Fig. 7.
Fig. 9, be frame structure schematic isometric in the utility model.
Figure 10, be the utility model user mode synoptic diagram (install cam lever and support body).
Figure 11, for the utility model user mode synoptic diagram (utilizing support body) with slide glass group corresponding states
Figure 12, be the utility model structural representation stereographic map.
Embodiment
As shown in Figure 3, Figure 4, the utility model comprises vertical casket seat 10 and is located at two groups of clipping assemblies 20 at casket seat 10 left and right two ends.
Casket seat 10 at least one sides are provided with opening recess 11 up as the side of both sides, are provided with bolster 12 in recess 11 peripheries.Bolster 12 can be rubber strip.Casket seat 10 left and right ends are respectively equipped with projection 101.Both sides, casket seat 10 top are respectively equipped with electrode contact 13, and are provided with the lead 14 that extends to casket seat 10 bottoms between two electrode contacts 13.
As shown in Figure 5, the clamp structure of two groups of clipping assemblies 20 that is arranged at casket seat 10 left and right two ends is identical, but direction is opposite.
Every group of clipping assembly 20 constitutes by opposite forward and backward two clamps 21 of structure equidirectional and in order to drive the knob 22 that two clamps 21 move.
Front side clamp 21 with two groups of clipping assemblies 20 is an example, as shown in Figure 3, two front side clamps 21 are respectively equipped with some correspondences and are sheathed on the casket seat 10 left and right two ends projections 101 and the guide channel 23 parallel with slide glass group 30 clipping directions and be skewed oblique pushing goove 24.
Knob 22 be provided with two corresponding respectively and stretch into the push rod 25 in the oblique pushing goove 24 on forward and backward two clamps 21.
Slide glass group 30 is for being provided with the pad of suitable thickness around the made thin slice of two sheet glass or acryl material, two plate sheets by clipping fixing after, sample solution and gel can be injected in the space between two plate sheets, to seal gel-filled effect.
During use, with casket seat 10 front sides is example, one group of slide glass group 30 is positioned over casket seat 10 front side recesses 11 and a left side, between the clipping assembly 20 front side clamps 21 of right two ends, cooperate again and rotate a left side, the knob 22 at right two ends, by stretching into push rod 25 in front side clamp 21 oblique pushing gooves 24 at oblique pushing goove 24 intrinsic displacements, to promote two clamp 21 displacements backward in front side, simultaneously, by the guiding that is sheathed on guide channel 23 on casket seat 10 projections 101, make the displacement backward synchronously of two clamps 21 in front side, and slide glass group 30 rearward recesses 11 and bolster 12 directions are pushed, make slide glass group 30 by closely by press fit, and be able to slide glass group 30 clippings be fixed, and prevent that effectively the slide glass group from moving for 30 pinches via simple rotation knob 22 action.When clamp 21 is pushed slide glass group 30, prevent that 30 pinches of slide glass groups from moving or too greatly slide glass group 30 is broken because of acting force by bolster 12.
When desire was taken out slide glass group 30, rotary knob 22 in the opposite direction, can make clamp 21 unclamp.
Former and later two clamp 21 structures of each group clipping assembly 20 are identical, and direction is opposite.
As shown in Figure 6, envelope is marked with the slide glass group 30 of sample and gel and the utility model of clipping slide glass group 30 places electrophoresis tank 40 together, and utilize two electrode contacts 13 at casket seat 10 tops to be connected with power lead 41, and damping fluid is being injected back turn-on power in the electrophoresis tank 40, can directly carry out DNA (deoxyribose nucleic acid), the separation of RNA (ribonucleic) and PROTEIN (protein) and the electrophoretic separation engineering of analysis, to save the fixing action of complicated slide glass, the global procedures of electrophoretic separation engineering is more simplified, and effectively keep the accuracy of electrophoretic separation engineering.
Because casket seat 10 is for inserting the member that electrophoresis tank 40 is carried out the electrophoresis engineering together with slide glass group 30, as Fig. 7, shown in Figure 8, can the passage 15 that unroll be set in casket seat 10 inside, and pipe adapter 16 be set, be connected for inlet pipeline 17 and outlet pipeline 18 in passage 15 two ends of unrolling.During operation, make chilled water, to reduce the working temperature of electrophoresis tank 40 inner conductive devices into and out of casket seat 10 inside.The design of casket seat 10 recesses 11 bottom surfaces is the both sides configuration gradually low towards the center, thereby can form the effect that bubble rises of quickening by its dip plane, with the bubble blocking-up electric action of avoiding damping fluid to be produced through the energising effect, and then effectively control the carrying out of electrophoretic separation engineering.
As Fig. 9, shown in Figure 10, outside casket seat 10 and two groups of clipping assemblies 20, can set support body 50, so as to slide glass group 30 apical margins being alignd and place, and be convenient to take in untapped casket seat 10 and clipping assembly 20 for members such as casket seat 10 and slide glass groups 30.
Support body 50 has the base plate of placing for casket seat 10 51, and the wall 52 that upright setting is enclosed corresponding to casket seat 10 frames on base plate 51, be provided with the raised line 56 of locating in order to guiding casket seat 10 in wall 52 interior sidewall surface, and on base plate 51, be provided with corresponding to 10 liang of side positions of casket seat and protrude out to the lug 53 of casket seat 10 end faces, lug 53 is provided with the axis hole 54 that wears for camshaft 55.Camshaft 55 is provided with cam 551 corresponding to slide glass group 30 apical margin positions, and base plate 51 is provided with elastic pad 57 corresponding to slide glass group 30 root edge positions.
When slide glass group 30 clipping is on casket seat 10, casket seat 10 can be positioned in the support body 50 together with slide glass group 30; As shown in figure 10, camshaft 55 is arranged in the axis hole 54 of forward and backward corresponding lug 53; As shown in figure 11, rotating cam axle 55, make camshaft 55 overhead cams 551 be pressed against slide glass group 30 tops, and with slide glass group 30 towards base plate 51 compressing, at this moment, the elastic pad of being located on the base plate 51 57 promptly produces elastic deformation because of the compressing of slide glass group 30, and form slide glass group 30 opposition of ejection upwards, so as to making slide glass group 30 bottoms be sealing state, and slide glass group 30 flushes with casket seat 10 end faces because apical margin is subjected to stopping of cam 551, to make things convenient for the envelope of slide glass group 30 before inserting electrophoresis tank 40 to annotate sample and gel operation, make the molecular linkage map of follow-up electrophoresis engineering gained more accurate.
When not carrying out the electrophoresis engineering, as shown in figure 12, also can utilize support body 50 to place casket seat 10 and slide glass group 30, and casket seat 10, clipping assembly 20 and slide glass group 30 can be concentrated, taken care of, to prevent to lose or suffer unnecessary collision, the base plate 51 that also can be engaged in support body 50 certainly is provided with the trepanning of planting for camshaft 55 58, so that concentrate, take care of together with camshaft 55.

Claims (9)

1, a kind of electrophoresis module of vertical type slide glass electrophoresis tank is characterized in that it comprises vertical casket seat and is located at two groups of clipping assemblies at casket, right two ends; The side of at least one side of casket seat is provided with opening recess up, is provided with bolster in the recess periphery; Casket, right-hand member are respectively equipped with projection; The clipping assembly comprises at least one clamp and the knob that moves in order to the drive clamp; Clamp be provided with some correspondences and be sheathed on the casket seat relative position place projection and with parallel guide channel and the oblique pushing goove of slide glass group clipping direction; Knob be provided with two corresponding respectively and stretch into the push rod in the oblique pushing goove on forward and backward two clamps.
2, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 1 is characterized in that described clipping assembly comprises two with respect to the opposite forward and backward clamp of the forward and backward side structure equidirectional of casket seat.
3, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 2 is characterized in that the side of described casket seat both sides is provided with opening recess up, is provided with bolster in the recess periphery.
4, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 1 is characterized in that both sides, described casket seat top are respectively equipped with the electrode contact that is connected with power lead, and is provided with the lead that extends to casket seat bottom between two electrode contacts.
5, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 1 is characterized in that described casket seat inside is provided with the passage that unrolls, and in the passage two ends of unrolling the pipe adapter for inlet pipeline and outlet pipeline linking is set.
6, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 1 is characterized in that the design of described casket seat recess bottom surface is the effect of rising with the acceleration bubble is gradually hanged down in both sides towards the center configuration.
7, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 1 is characterized in that can setting support body outside described casket seat and two groups of clipping assemblies; Support body has the base plate of placing for the casket seat, upright on the base plate wall that encloses corresponding to casket seat frame is set, and is provided with corresponding to casket seat two side positions on base plate and protrudes out to the lug of casket seat end face, and lug is provided with the axis hole that wears for camshaft; Camshaft is provided with cam corresponding to slide glass group apical margin position, and base plate is provided with elastic pad corresponding to slide glass group root edge position.
8, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 7 is characterized in that being provided with on the described support body base plate trepanning of planting for camshaft.
9, the electrophoresis module of vertical type slide glass electrophoresis tank according to claim 7 is characterized in that described support body wall interior sidewall surface is provided with the raised line in order to guiding casket seat location.
CN 200320104301 2003-11-06 2003-11-06 Electrophoresis mould set having vertical carrier piece electrophoretic groove Expired - Lifetime CN2658746Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200320104301 CN2658746Y (en) 2003-11-06 2003-11-06 Electrophoresis mould set having vertical carrier piece electrophoretic groove

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200320104301 CN2658746Y (en) 2003-11-06 2003-11-06 Electrophoresis mould set having vertical carrier piece electrophoretic groove

Publications (1)

Publication Number Publication Date
CN2658746Y true CN2658746Y (en) 2004-11-24

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106233130A (en) * 2014-11-20 2016-12-14 夏普株式会社 Organism analysis of molecules device
CN114441260A (en) * 2017-11-27 2022-05-06 徕卡生物系统成像股份有限公司 Slide rack determination system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106233130A (en) * 2014-11-20 2016-12-14 夏普株式会社 Organism analysis of molecules device
CN106233130B (en) * 2014-11-20 2018-11-20 夏普生命科学株式会社 Organism molecule analytical equipment
CN114441260A (en) * 2017-11-27 2022-05-06 徕卡生物系统成像股份有限公司 Slide rack determination system

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GR01 Patent grant
C17 Cessation of patent right
CX01 Expiry of patent term

Expiration termination date: 20131106

Granted publication date: 20041124