CN2604688Y - Clonorchis sinensis bladder worm immunocolloidal gold detecting box - Google Patents
Clonorchis sinensis bladder worm immunocolloidal gold detecting box Download PDFInfo
- Publication number
- CN2604688Y CN2604688Y CN 03224470 CN03224470U CN2604688Y CN 2604688 Y CN2604688 Y CN 2604688Y CN 03224470 CN03224470 CN 03224470 CN 03224470 U CN03224470 U CN 03224470U CN 2604688 Y CN2604688 Y CN 2604688Y
- Authority
- CN
- China
- Prior art keywords
- clonorchis sinensis
- metacercaria
- sample
- line
- cobbold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 19
- 241001327965 Clonorchis sinensis Species 0.000 title claims abstract description 17
- 239000010931 gold Substances 0.000 title claims description 16
- 229910052737 gold Inorganic materials 0.000 title claims description 16
- 239000000427 antigen Substances 0.000 claims abstract description 14
- 102000036639 antigens Human genes 0.000 claims abstract description 14
- 108091007433 antigens Proteins 0.000 claims abstract description 14
- 238000003908 quality control method Methods 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 239000003292 glue Substances 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 claims description 17
- 239000000084 colloidal system Substances 0.000 claims description 15
- 241000754688 Cercaria Species 0.000 claims description 11
- 241000242722 Cestoda Species 0.000 claims description 11
- 241000242678 Schistosoma Species 0.000 claims description 11
- 210000004706 scrotum Anatomy 0.000 claims description 11
- 239000012790 adhesive layer Substances 0.000 claims description 4
- 229920000297 Rayon Polymers 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims 1
- 229920000715 Mucilage Polymers 0.000 abstract 1
- 239000000853 adhesive Substances 0.000 abstract 1
- 239000002390 adhesive tape Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 208000030852 Parasitic disease Diseases 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 210000000013 bile duct Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 206010009344 Clonorchiasis Diseases 0.000 description 1
- 241000242711 Fasciola hepatica Species 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 239000004836 Glue Stick Substances 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 210000003459 common hepatic duct Anatomy 0.000 description 1
- 208000006275 fascioliasis Diseases 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
An immune colloidal gold test box is used for clonorchis sinensis cobbold metacercaria. One side of the box cover is provided an opening of an ellipse or a near circular sample-adding area; a strip shape observing window is provided in the middle of the box cover; a piece of test paper adheres on the bottom of a plastic box base through a double-side adhesive tape or a level composed of mucilage glue; the bottom level of the test paper is plastic slice; one side of the upper level of test paper corresponding the sample-adding hole has antibody sample-adding area for clonorchis sinensis cobbold metacercaria specificity IgG antibody, absorbing indicating reagents of colloidal gold labels; the middle observing window spot corresponding the box cover is the reaction joint area, which is a chromatography film, on which the test line T, added with the test reagents of single clone or multiple clone IgG antibody ingredient of the antigen specificity of the clonorchis sinensis cobbold metacercaria, is arranged; also arranged is a quality-control line C which is added with quality-control reagents of excretory-secretory antigen of clonorchis sinensis cobbold metacercaria;, as well as the one added quality-control reagent is the quality-control line C of excretory-secretory antigen of clonorchis sinensis cobbold metacercaria, the test line T is on the near side of sample-adding area, and the quality-control line C is on the far side of sample-adding area. The utility model provides a technological means which is sensitive, special, simple, fast, inexpensive, and suitable for field application.
Description
Technical field
The utility model relates to a kind of immune colloid gold and detects box, and especially the encysted metacercaria of clonorchis sinensis immune colloid gold detects box.
Background technology
Liver rot is a kind of infecting both domestic animals and human parasitic disease, is that bladder worm grows due to the adult in hepatic duct after having eaten the living fish of an infection China scrotum larva of a tapeworm or the cercaria of a schistosome.Clonorchis sinensis can long term survival in bile duct, the longlyest reaches more than 30 year, not only bile duct is caused obstruction, also can cause inflammatory reaction, hepatapostema, cirrhosis even liver cancer.Liver rot is one of China's common parasitic disease, and the whole nation has more than 1,000 ten thousand liver fluke patients approximately, especially likes the area, Guangdong and Guangxi Provinces of eating living fish (sashimi (raw fish)), and the incidence of disease is higher.Intermediate host fish INFECTION IN DETECTION seems very important to its preventing and controlling.Ratio and infectiosity that fish infect bladder worm are to estimate an important indicator of popular situation of clonorchiasis and trend.It also is the important content that Food Hygiene Surveillance detects.At present fish are infected the detection of bladder worm, still lack the specific means that detect.
Technology contents
The purpose of this utility model is exactly for the technological means of a kind of sensitivity, special, easy, quick, cheap, suitable rig-site utilization is provided.
The utility model is to realize like this.
The utility model adopts a small plastic box, and small plastic box has lid and base, and a side of lid has oval or rotund well for adding sample to be checked; The middle part of lid has the view window of strip; The bottom of test strips is a plastic tab, one side of the corresponding lid well in its upper strata be have high capacity thieving paper be adsorbed with indicator be China of colloid gold label prop up the special IgG antibody of the scrotum larva of a tapeworm or the cercaria of a schistosome sample application zone, the middle part of corresponding lid view window is the reaction bonded district, be chromatographic film, which is provided with detection line and nature controlling line, wherein detection line is in the sample adding area side, and nature controlling line is in sample application zone side far away, and the detectable on the detection line is the monoclonal or the polyclone IgG antibody of encysted metacercaria of clonorchis sinensis antigen-specific.The aperture of detection line is 5～8 μ m, and the quality control reagent on the nature controlling line is that antigen is drained in the secretion of China's scrotum larva of a tapeworm or the cercaria of a schistosome; Offside one end of the sample application zone of test strips can be provided with the suction zones of high capacity thieving paper.The adhesive-layer that test strips of the present utility model is formed by double sticky tape or viscose glue sticks on the plastic casing base.
The China of indicator colloid gold label of the present utility model props up scrotum larva of a tapeworm or the cercaria of a schistosome specific IgG antibodies and is added in the position of sample application zone near chromatographic film.
The aperture in reaction bonded of the present utility model district (chromatographic film) is 5～8 μ m, can adopt cellulose mixture or cellulose nitrate chromatographic film.
During use, serum of a fish or whole blood on sample application zone is dripped, wait whole suctions after, drip two PBS-Tween 20 phosphate buffers, after about 2～3 minutes, from view window, can observe, occur a red line on the nature controlling line, a red line also can appear in positive on detection line.
When encysted metacercaria of clonorchis sinensis immune colloid gold fast detecting box of the present utility model detects, to as if China's scrotum larva of a tapeworm or the cercaria of a schistosome be secreted into antigen in the fish blood, can reflect that the existing disease of bladder worm infects, have susceptibility height, high specificity, easy and simple to handle, reaction fast, trace is accurate, economical and practical, be convenient to preserve, be fit to advantages such as on-the-spot use.
Description of drawings
Fig. 1 is a synoptic diagram of opening small plastic box of the present utility model.
Fig. 2 is a synoptic diagram of the present utility model.
Among the figure, (1) is lid, and (2) are base, and (3) are well, and (4) are view window, and (5) are test strips, and (6) are adhesive-layer, (7) sample application zone, (8) detection line T, (9) nature controlling line C, (10) suction zones.
Embodiment
As shown in Figure 2, the utility model adopts a small plastic box, and a side of lid has oval or rotund sample application zone (3), for adding sample to be checked; The middle part of lid has the view window (4) of a strip.At the another side of lid, can be used as the title of mark district in order to labelling kit.
As shown in Figure 1, small plastic box of the present utility model has lid (1) and base (2), wherein test strips (5) sticks on the plastic casing base (2) by the adhesive-layer (6) of double sticky tape or viscose glue composition, the test strips bottom is a plastic tab, one side of the corresponding lid well in its upper strata is that the indicator that is adsorbed with of high capacity thieving paper is arranged is the sample application zone (7) that scrotum larva of a tapeworm or the cercaria of a schistosome specific IgG antibodies props up in China of colloid gold label, the view window position, middle part of corresponding lid is the reaction bonded district, be chromatographic film, which is provided with detectable is encysted metacercaria of clonorchis sinensis antigentic specificity monoclonal or polyclonal IgG detection of antibodies line T (8), and be provided with the nature controlling line C (9) of quality control reagent for the secretion antigen of China's scrotum larva of a tapeworm or the cercaria of a schistosome, wherein detection line T (8) is in sample adding area (7) side, and nature controlling line C (9) is in sample application zone (7) side far away.
The aperture of detection line of the present utility model is 5～8 μ m.
The aperture in reaction bonded of the present utility model district is 5～8 μ m.
The offside of the sample application zone of test strips of the present utility model (7) can be provided with the suction zones (10) of high capacity thieving paper.
During use, fish serum or whole blood on sample application zone is dripped, after whole the suction, dripping two droppings has PBS-Tween 20 phosphate buffers, and the IgG antibody of colloid gold label and the antigen in the sample form antigen one antibody colloid gold immune complex, through launching, be fixed on the antibodies of an another kind of China scrotum larva of a tapeworm or the cercaria of a schistosome antigen on the detection line, in the enrichment of detection line place, this is that detection line shows red (the utility model adopts the double antibodies sandwich method) owing to formed the colloidal gold antigen antibody ligand; And scrotum larva of a tapeworm or the cercaria of a schistosome antibody continuation reach is propped up by the China of free colloid gold label, and enrichment on the nature controlling line that is fixed with bladder worm antigen makes nature controlling line show red; The nature controlling line colour developing shows the up-to-standard of kit.
Claims (4)
1, a kind of encysted metacercaria of clonorchis sinensis immune colloid gold detects box, one side of lid has oval or rotund sample application zone, the middle part of lid has the view window of a strip, capsule has lid and base, it is characterized in that sticking on the plastic casing base by the adhesive-layer that test strips is formed by double sticky tape or viscose glue, the test strips bottom is a plastic tab, side of corresponding lid well is that the indicator that is adsorbed with of high capacity thieving paper is arranged is the sample application zone that scrotum larva of a tapeworm or the cercaria of a schistosome specific IgG antibodies props up in China of colloid gold label on it, the view window position, middle part of corresponding lid is the reaction bonded district, be chromatographic film, which is provided with monoclonal or polyclone IgG detection of antibodies line T that detectable is the encysted metacercaria of clonorchis sinensis antigen-specific, and be added with quality control reagent is drained antigen for the secretion of China's scrotum larva of a tapeworm or the cercaria of a schistosome nature controlling line C, wherein detection line T is in the sample adding area side, and nature controlling line C is in sample application zone side far away.
2, encysted metacercaria of clonorchis sinensis immune colloid gold according to claim 1 detects box, and the aperture that it is characterized in that detection line is 5～8 μ m.
3, encysted metacercaria of clonorchis sinensis immune colloid gold according to claim 1 detects box, and the aperture that it is characterized in that the reaction bonded district is 5～8 μ m.
4, encysted metacercaria of clonorchis sinensis immune colloid gold according to claim 1 detects box, it is characterized in that the offside of sample application zone is provided with the suction zones of high capacity thieving paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03224470 CN2604688Y (en) | 2003-03-24 | 2003-03-24 | Clonorchis sinensis bladder worm immunocolloidal gold detecting box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03224470 CN2604688Y (en) | 2003-03-24 | 2003-03-24 | Clonorchis sinensis bladder worm immunocolloidal gold detecting box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2604688Y true CN2604688Y (en) | 2004-02-25 |
Family
ID=34163379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03224470 Expired - Fee Related CN2604688Y (en) | 2003-03-24 | 2003-03-24 | Clonorchis sinensis bladder worm immunocolloidal gold detecting box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2604688Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101266252A (en) * | 2008-04-25 | 2008-09-17 | 中国人民解放军第三军医大学 | Paragonimiasis detection device and method for making same |
| CN102250231A (en) * | 2010-05-19 | 2011-11-23 | 中国疾病预防控制中心寄生虫病预防控制所 | Chonorchis sinensis specific PPMP antigens and application thereof |
| CN101387641B (en) * | 2007-09-11 | 2013-11-27 | 深圳市康百得生物科技有限公司 | Liver fluke rapid immune detection kit for fresh water fishes and detection method |
| CN107782900A (en) * | 2016-08-31 | 2018-03-09 | 朱海燕 | The Test paper component of human growth and differentiation factor 7 15 |
-
2003
- 2003-03-24 CN CN 03224470 patent/CN2604688Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101387641B (en) * | 2007-09-11 | 2013-11-27 | 深圳市康百得生物科技有限公司 | Liver fluke rapid immune detection kit for fresh water fishes and detection method |
| CN101266252A (en) * | 2008-04-25 | 2008-09-17 | 中国人民解放军第三军医大学 | Paragonimiasis detection device and method for making same |
| CN102250231A (en) * | 2010-05-19 | 2011-11-23 | 中国疾病预防控制中心寄生虫病预防控制所 | Chonorchis sinensis specific PPMP antigens and application thereof |
| CN102250231B (en) * | 2010-05-19 | 2014-09-10 | 中国疾病预防控制中心寄生虫病预防控制所 | Chonorchis sinensis specific PPMP antigens and application thereof |
| CN107782900A (en) * | 2016-08-31 | 2018-03-09 | 朱海燕 | The Test paper component of human growth and differentiation factor 7 15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |