CN2582367Y - Gene proliferation hybridization apparatus - Google Patents
Gene proliferation hybridization apparatus Download PDFInfo
- Publication number
- CN2582367Y CN2582367Y CN 02290382 CN02290382U CN2582367Y CN 2582367 Y CN2582367 Y CN 2582367Y CN 02290382 CN02290382 CN 02290382 CN 02290382 U CN02290382 U CN 02290382U CN 2582367 Y CN2582367 Y CN 2582367Y
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- hybridization
- unit
- gene
- cup
- proliferation
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Abstract
The utility model discloses a gene proliferation hybridization apparatus which belongs to a measurement apparatus in the field of biology and medicine, particularly to a measurement apparatus which is used for gene proliferation, hybridization, gene chips and protein chips. A hybridization unit and a gene proliferation unit are arranged in a same machine housing, and the hybridization unit is mainly composed of a hybridization cup, a heat conducting block, an ultraviolet lamp, a rocking bed, a liquid suction pump, a collection bottle and a sensor. Each sensor which is arranged in the gene proliferation unit and the hybridization unit is connected with a sharing automatic control circuit system, and the terminal of the sharing automatic control circuit system is a sharing parameter display screen and an operation keyboard. The apparatus integrates functions of a gene proliferation apparatus, a hybridization apparatus and a gene detecting apparatus so as to not only save experiment space and equipment investment, but also shorten experimental period and avoid complicated, excessive and tedious measuring process in the prior art because of the online operation and the automatic sampling of PCR proliferation and hybridization reaction. The apparatus can store lots of experiment data and carry out data analysis in the method of online with a reading apparatus.
Description
Technical field
The utility model belongs to the determining instrument of a kind of biology, medical domain, especially a kind of determining instrument that is used for gene magnification, hybridization, genetic chip, protein chip.
Background technology
Development along with human sciences's technology, understanding to gene is more and more deep, the project that a series of new need such as genetic chip, protein chip, gene magnification, DAN dot hybridization are measured emerges, and has therefore also constantly produced the determining instrument that adapts therewith.Chinese patent ZL98244367.6 discloses a kind of " gene-amplificative instrament " (one of the applicant is the patentee), it make gene amplification reaction be polymerase chain reaction (PCR) can be in safety under the automatic control, contamination-freely carry out fast; But this instrument has only this function.The hybridization aspect, the domestic and international mensuration means that are mostly to continue to use traditional craft or half instrumentation, this situation is unfavorable for the development of gene technology.Chinese patent ZL 02228396.X discloses a kind of " autoabstract reaction cup " (several workers of one of the applicant are patentees), it be a kind of inside of deft design be provided with channel system can be under the negative pressure source effect molecular hyridization reaction cup of Automatic Extraction reactant liquor, its appearance for the hybridization reaction determining instrumentization, abandon the sample automation feasible basis be provided.In addition, need gene magnification and hybridization simultaneously, intersect, measure in sequence and also press for a kind of that can meet the demands, easy to operate comprehensive instrument.
Summary of the invention
At the above-mentioned deficiency of prior art, the purpose of this utility model provides a kind of gene magnification hybridization instrument, and it can carry out the mensuration of gene magnification, hybridization reaction, can also carry out the test of genetic chip, protein chip; And all mensuration is to be finished by the automatic operation system control of microcomputerized control.
The purpose of this utility model is achieved in that the gene magnification hybridization instrument contains gene magnification unit and hybridization unit, alternating temperature assembly in the gene magnification unit is close to device from top to bottom successively by the alternating temperature metal derby that is inserted with some test tubes, flexible heater film, semiconductor chilling plate, air-cooled radiator and is formed, and alternating temperature metal derby top is provided with the heat lid that covers test tube; At the alternating temperature assembly apparatus temperature sensor is arranged; Its special feature is: the critical piece of hybridization unit and gene magnification unit is arranged in the same casing, and shared microcomputer automatic control system and shared display screen and operation keyboard are set; The hybridization unit mainly is made up of hybridization cup, heat-conducting block, uviol lamp, shaking table, suction pumps, receiving flask and sensor, the hybridization cup places on the heat-conducting block, heat-conducting block is installed on the shaking table, the uviol lamp and the cabinet cover that are arranged at hybridization cup top link together, suction pumps is arranged on the below of casing, and receiving flask is arranged at outside the casing; The liquid outlet of hybridization cup links to each other with an outlet of receiving flask, and another outlet of receiving flask links to each other with suction pumps, the silencer in addition that links to each other with suction pumps; Be arranged at gene magnification unit and hybridization each sensor in the unit and link to each other with shared automatic control circuit system, the terminal of shared automatic control system is common parameter display screen and operation keyboard.
Described hybridization cup be a kind of have the internal water channel system can be under the negative pressure source effect reaction cup of Automatic Extraction reactant liquor.
Before the utility model, though had gene-amplificative instrament and simple hybridization instrument to occur, the utility model is not their simple combination.Hybridization instrument before the utility model is half instrumentation, a lot of operations all still are manual mode, only after the invention of autoabstract reaction cup is made, because the ingenious design of its channel structure just makes automatic operation become possibility, the utility model has utilized the above-mentioned achievement of the prior art gene magnification hybridization instrument that just designed success just.Its technique effect is very outstanding: 1, this instrument is integrated in one the function of gene-amplificative instrament, hybridization instrument, gene-detecting apparatus, lab space and equipment investment have not only been saved, and pcr amplification and hybridization reaction on-line execute, autoabstract, shorten experimental period greatly, avoided complicated, loaded down with trivial details, tediously long in the past mensuration process.2, its instrumentation of having realized hybridization portion with abandon sample automation (do similar experiment both at home and abroad and be manual or half instrumentation), not only simplified operation, and more convenient, shortened experimental period simultaneously, the data of experiment are more objective.3, a complete set of instrument not only has PCR and hybridization function, also has genetic chip and protein chip measuring ability and scientific research function.4, this instrument uses same software operation, uses the common parameter display screen, and the exquisiteness design of display interfaces makes easy and simple to handle understandable.5, this instrument and reading apparatus are online can store a large amount of experimental datas and carry out data analysis.
Description of drawings
Accompanying drawing 1 is this apparatus structure schematic diagram;
Accompanying drawing 2 is schematic top plan view that this instrument removes loam cake;
Accompanying drawing 3 is hybridization cellular construction schematic diagrames;
Accompanying drawing 4 is pipeline connection diagrams.
Accompanying drawing acceptance of the bid keep the score state as follows: 1---casing; 2---loam cake; 3---the hybridization unit; 4---the gene magnification unit; 5---the hybridization cup; 6---heat-conducting block; 7---uviol lamp; 8---velocity sensor; 9---receiving flask; 10---shaking table; 11---shared display screen and operation keyboard; 12---shared automatic control system; 13---the gene magnification test tube; 14---clamp nut; 15---suction pumps; 16---silencer; 17---transit joint.
Embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is described in further detail.As shown in accompanying drawing 1,2, hybridization unit 3 of this instrument and gene magnification unit 4 are arranged at the both sides of casing 1, and the front end of casing 1 is provided with shared display screen and operation keyboard 11.Open casing upper cover 2 and can see the test tube 13 of gene magnification unit and the hybridization cup 5 of hybridization unit.The receiving flask of hybridization unit is arranged at outside the casing, and shared automatic control system 12 devices are in the front portion of casing.As shown in accompanying drawing 3, the hybridization cup 5 of hybridization unit 3 is installed on the heat-conducting block 6, and heat-conducting block 6 is arranged on the shaking table 10, and what link to each other with shaking table 10 goes back speed sensor 8, the hybridization cup is connected with heat-conducting block 6 usefulness clamp nuts 14, and suction pumps 15 is arranged on the below of casing 1.Pipeline connects as shown in Figure 4, hybridization cup 5 liquid outlets join by the interface of transit joint 17 with receiving flask 9, another joint of receiving flask 9 is connected with suction pumps (vavuum pump) 15 by transit joint 17, the silencer 16 in addition that links to each other with suction pumps 15.Because the use more complicated of instrument is not given unnecessary details at this.
Claims (2)
1, a kind of gene magnification hybridization instrument, contain gene magnification unit and hybridization unit, alternating temperature assembly in the gene magnification unit is close to device from top to bottom successively by the alternating temperature metal derby that inserts some test tubes, flexible heater film, semiconductor chilling plate, air-cooled radiator and is formed, and alternating temperature metal derby top is provided with the heat lid that covers test tube; Device has temperature sensor in the alternating temperature assembly; It is characterized in that: the critical piece of hybridization unit (3) and gene magnification unit (4) is arranged in the same casing (1) their shared same microcomputer automatic control systems (12) and shared display screen and operation keyboard (11); Hybridization unit (3) mainly is made up of hybridization cup (5), heat-conducting block (6), uviol lamp (7), shaking table (10), suction pumps (15), receiving flask (9) and sensor, hybridization cup (5) is installed on the heat-conducting block (6) that is driven by shaking table (10), uviol lamp (7) is arranged at hybridization cup (5) top and links together with cabinet cover (2), suction pumps (15) is arranged on the below of casing (1), and receiving flask (9) is arranged at outside the casing (1); The liquid outlet of hybridization cup (5) links to each other with an outlet of receiving flask (9), and another outlet of receiving flask (9) links to each other with suction pumps (15), the silencer (16) in addition that links to each other with suction pumps (15); Each sensor that is arranged at gene magnification unit (4) and hybridization unit (3) links to each other with corresponding shared automatic control circuit system (12), and the terminal of shared automatic control system (12) is common parameter display screen and operation keyboard (11).
2, instrument according to claim 1 is characterized in that: described hybridization cup (5) be a kind of have the internal water channel system can be under the negative pressure source effect reaction cup of Automatic Extraction reactant liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02290382 CN2582367Y (en) | 2002-12-13 | 2002-12-13 | Gene proliferation hybridization apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02290382 CN2582367Y (en) | 2002-12-13 | 2002-12-13 | Gene proliferation hybridization apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2582367Y true CN2582367Y (en) | 2003-10-29 |
Family
ID=33749623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02290382 Expired - Fee Related CN2582367Y (en) | 2002-12-13 | 2002-12-13 | Gene proliferation hybridization apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2582367Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN101942385B (en) * | 2009-07-06 | 2013-08-14 | 中山大学达安基因股份有限公司 | Full-automatic hybridization oven and implementation method and application thereof |
-
2002
- 2002-12-13 CN CN 02290382 patent/CN2582367Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942385B (en) * | 2009-07-06 | 2013-08-14 | 中山大学达安基因股份有限公司 | Full-automatic hybridization oven and implementation method and application thereof |
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN102134552B (en) * | 2010-12-31 | 2014-05-21 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |