CN2581995Y - Gene chip colour developing device - Google Patents
Gene chip colour developing device Download PDFInfo
- Publication number
- CN2581995Y CN2581995Y CN 02260322 CN02260322U CN2581995Y CN 2581995 Y CN2581995 Y CN 2581995Y CN 02260322 CN02260322 CN 02260322 CN 02260322 U CN02260322 U CN 02260322U CN 2581995 Y CN2581995 Y CN 2581995Y
- Authority
- CN
- China
- Prior art keywords
- colour developing
- genetic chip
- gene chip
- display device
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a gene chip colour showing device, which comprises a gene chip which is provided with an enzyme label. The utility model is characterized in that the utility model also comprises a colour showing base piece which is composed of a flat type supporting carrier, a film layer which is fixedly connected with the flat type supporting carrier and a colour showing liquid layer which covers the film layer. A detecting area of the gene chip with an enzyme label and the colour showing liquid layer of the colour showing base piece are arranged oppositely,. The utility model cause the enzyme catalysis of the gene chip to generate colouring deposits which are adsorbed and fixed by the film layer. Furthermore, because the adsorbing and fixing ability of the film layer is greater than the adsorbing and fixing ability of the rigid flat surface of the gene chip, a colour showing result of the film layer is better than a direct colour showing result of the rigid flat surface of the gene chip. Furthermore, the colour showing result of the film layer and the colour showing result of the gene chip are in a mirror image relationship. Therefore, the utility model can realize an ideal colour showing detecting result of the gene chip.
Description
(1) technical field
The utility model relates to a kind of genetic chip display device.
(2) background technology
Biochip technology is a kind ofly simultaneously a large amount of gene probe molecules to be fixed on the support, by making nucleic acid molecular hybridization pairing characteristic, carry out hybridization reaction with the determined nucleic acid molecule of tape label and method that the sequence information of DNA sample is efficiently understood and analyzed.Biochip technology is in detection in Gene Mutation, and the medical diagnosis on disease aspect that Polymorphism Analysis is relevant obtains to use.
At present the method that nucleic acid molecules to be measured is carried out mark mainly contains fluorescence labeling (as Cy3, Cy5, fluorescein, Dexas Red etc.).Adopt fluorescence labeling, gene chip hybridization result's detection need be adopted fluorescence detection device (as laser co-focusing fluorescent scanning instrument), but this equipment price costliness.And employing digoxin (DIG), during biotin (Biotin) mark, can utilize DigiTAb alkaline phosphatase (anti-DIG-AP) compound or DigiTAb horseradish peroxidase (anti-DIG-HRP) compound, streptavidin alkaline phosphatase multienzyme complex (SA-AP) or streptavidin horseradish peroxidase (SA-HRP) compound respectively with digoxin and biotin reaction after, have alkaline phosphatase (AP) or horseradish peroxidase (HRP) mark on the probe points that makes genetic chip take place effectively to hybridize, be called for short " genetic chip that has enzyme labeling ".Alkaline phosphatase (AP) or horseradish peroxidase (HRP) catalysis respectively contain the solution of tetrazole indigo plant (NBT) and 5-bromo-4-chloro-3-indoxyl-phosphoric acid-4-toluene amine salt (BCIP) and contain the solution generation chromogenic reaction of tetramethyl benzidine (TMB), produce coloured precipitation and the gene chip hybridization result is detected.But because genetic chip is normally made probe points on rigid carriers such as microslide, silicon chip, these rigid carriers are to the absorption set ability of coloured precipitation, and the result is undesirable in colour developing.
(3) utility model content
The purpose of this utility model provides a kind of display device that can improve genetic chip colour developing result.
A kind of genetic chip display device provided by the utility model, comprise that one has the genetic chip of enzyme labeling, be characterized in: it also comprises a colour developing substrate, described colour developing substrate by the plane prop carrier, on this plane prop carrier affixed rete and on this rete, cover the colour developing liquid layer of putting and formed, the surveyed area that described genetic chip has an enzyme labeling is provided with in opposite directions with the colour developing liquid layer of colour developing substrate.
Above-mentioned genetic chip display device, wherein, the plane branching carrier can adopt rigid carrier, that is: glass sheet, or silicon chip, or sheet metal.
Above-mentioned genetic chip display device, wherein, the sheet material that the plane branching carrier can adopt macromolecule polymeric material to make, or wood chip, or bamboo chip.
Above-mentioned genetic chip display device, wherein, rete is a kind of nylon membrane, or a kind of cellulose membrane.
Above-mentioned genetic chip display device, wherein, the colour developing liquid in the colour developing liquid layer can adopt the solution that contains tetramethyl benzidine, and the enzyme on the described genetic chip is a horseradish peroxidase.
Above-mentioned genetic chip display device, wherein, the colour developing liquid in the colour developing liquid layer can adopt the solution that contains tetrazole indigo plant and 5-bromo-4-chloro-3-indoxyl-phosphoric acid-4-toluene amine salt, and the enzyme on the described genetic chip is an alkaline phosphatase.
Adopted above-mentioned technical solution, coloured precipitation tunicle absorption set that enzymatic on the genetic chip produces, because the absorption set ability of rete is much larger than the genetic chip rigid plane, therefore its colour developing result is better than the direct colour developing on the genetic chip rigid plane, and become mirror with colour developing result on the genetic chip, so the utility model genetic chip color developing detection result that can realize ideal in the colour developing result on the rete.
(4) description of drawings
Fig. 1 is the structural representation of the utility model genetic chip display device;
Fig. 2 is that the utility model colour developing substrate is used for one of result of genetic chip color developing detection.
(5) embodiment
As shown in Figure 1, display device is made up of colour developing substrate 1 and the genetic chip 2 that has enzyme labeling, colour developing substrate 1 by plane prop carrier 11, on this plane prop carrier affixed rete 12 and on this rete, cover the colour developing liquid layer of putting 13 and is formed, the surveyed area 21 that described genetic chip 2 has an enzyme labeling is provided with in opposite directions with the colour developing liquid layer 13 of the substrate that develops the color.
At commonly used microslide, silicon chip, sheet metal or macromolecule polymeric material (as PVC, PP, on the plane of plane branching carriers 11 such as sheet material of organic glass etc.) making and wood chip, bamboo chip, paste the suitably nylon membrane or the cellulose rete 12 of size with strengthened water proof glue (as acrylic compounds).
Genetic chip 2 is gene probe arrays of making by Design Mode point on aldehyde group modified microslide, makes after fixing operation.
The genetic chip colour developing is by the colour developing liquid layer 13 on the film 12 that is covered at plane prop carrier 11, then will be facing down with the genetic chip 2 of enzyme labeling, make the surveyed area 21 of genetic chip 2 aim at colour developing liquid layer on the retes 12, lid is placed certain hour thereon under the proper temperature.
The result of genetic chip color developing detection that shown in Figure 2 is pastes nylon membrane to make the colour developing substrate on common microslide, oligonucleotides and the complementary oligonucleotide hybridization of point on genetic chip with digoxigenin labeled react with DigiTAb alkaline phosphatase multienzyme complex then.On the colour developing substrate, drip the solution that contains tetrazole indigo plant (NBT) and 5-bromo-4-chloro-3-indoxyl-phosphoric acid-4-toluene amine salt (BCIP), then will be facing down with the genetic chip of enzyme labeling, make the surveyed area 21 of genetic chip 2 aim at the film 12 that is added with colour developing liquid, lid is placed 0.5 hour result thereon, and the result scans under 1600dpi resolution with flat bed scanner and obtains.
Claims (6)
1. genetic chip display device, comprise that one has the genetic chip of enzyme labeling, its feature as for: it also comprise one the colour developing substrate, described colour developing substrate by the plane prop carrier, on this plane prop carrier affixed rete and on this rete, cover the colour developing liquid layer of putting and formed, the surveyed area that described genetic chip has an enzyme labeling is provided with in opposite directions with the colour developing liquid layer of colour developing substrate.
2. as claim 1 described genetic chip display device, it is characterized in that described plane branching carrier can adopt rigid carrier, that is: glass sheet, or silicon chip, or sheet metal.
3. as claim 1 described genetic chip display device, it is characterized in that the sheet material that described plane branching carrier can adopt macromolecule polymeric material to make, or wood chip, or bamboo chip.
4. as claim 1 described genetic chip display device, it is characterized in that described rete is a kind of nylon membrane, or a kind of cellulose membrane.
5. as claim 1 described genetic chip display device, it is characterized in that the colour developing liquid in the described colour developing liquid layer can adopt the solution that contains tetramethyl benzidine, the enzyme on the described genetic chip is a horseradish peroxidase.
6. as claim 1 described genetic chip display device, it is characterized in that, colour developing liquid in the described colour developing liquid layer can adopt the solution that contains tetrazole indigo plant and 5-bromo-4-chloro-3-indoxyl-phosphoric acid-4-toluene amine salt, and the enzyme on the described genetic chip is an alkaline phosphatase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02260322 CN2581995Y (en) | 2002-09-27 | 2002-09-27 | Gene chip colour developing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02260322 CN2581995Y (en) | 2002-09-27 | 2002-09-27 | Gene chip colour developing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2581995Y true CN2581995Y (en) | 2003-10-22 |
Family
ID=33727405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02260322 Expired - Lifetime CN2581995Y (en) | 2002-09-27 | 2002-09-27 | Gene chip colour developing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2581995Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774628B (en) * | 2003-04-16 | 2011-04-13 | 爱科来株式会社 | Analyzing tool being reduced in distance of diffusion of reagent and method for manufacture thereof |
-
2002
- 2002-09-27 CN CN 02260322 patent/CN2581995Y/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774628B (en) * | 2003-04-16 | 2011-04-13 | 爱科来株式会社 | Analyzing tool being reduced in distance of diffusion of reagent and method for manufacture thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2321638C2 (en) | Method for preparing multifunctional multichip, multichip for successive or parallel screening biopolymers, method for analysis of biopolymers and set for realization of method | |
| KR100784437B1 (en) | Composition of Sol for Sol-Gel Biochip to Immobilize Probe On Substrate Without Surface Treatment and Method for Screening Thereof | |
| AU2003242605A1 (en) | Novel high density arrays and methods for analyte analysis | |
| EP1430154B1 (en) | Enzyme-amplified redox microarray detection process | |
| CN1636137A (en) | Microarray method of genotyping multiple samples at multiple loci | |
| JP2004526420A (en) | Immobilization of biopolymers to aminated substrates by direct adsorption | |
| US20020182603A1 (en) | Uniformly functionalized surfaces for microarrays | |
| CN2581995Y (en) | Gene chip colour developing device | |
| CN1926244B (en) | Analysis chip with reference scale, kits and analytical methods | |
| Marcy et al. | Innovative integrated system for real-time measurement of hybridization and melting on standard format microarrays | |
| US20090269737A1 (en) | Integrated non-homogeneous nucleic acid amplification and detection | |
| CN1448500A (en) | Flushing-free PCR amplification tube capable of directly detecting gene | |
| CN101162201A (en) | TSA- nano-gold making silver-staining testing method of gene chip | |
| JP2004537722A (en) | Detection method for detecting the formation or hybridization of a complex between at least two basic molecules on a solid support based on an amplified signal on the support | |
| US20020076832A1 (en) | Method of attaching a biopolymer to a solid support | |
| CN1263843C (en) | PCR amplifieation tube in use for multistep reaction | |
| CN2615141Y (en) | Non-flushing PCR amplificating tube capable of directly detecting gene | |
| JP2003207505A (en) | Analyzing method of biological molecule | |
| CN2483395Y (en) | Capillary bio-chip device | |
| RU69866U1 (en) | BIOCHIP | |
| CN2649597Y (en) | PCR amplification tube for multi-step reaction | |
| CN100381579C (en) | Nonspecific bonding probe removal method through electrophoresis in biological chip hybridization | |
| Wang et al. | Heriot-Watt University | |
| CN1721547A (en) | Detection of transcription factor protein by double chain RNA molecule with special labeling fixed to micro-plate coated by exonuclease III | |
| CN2751028Y (en) | Suspended biochip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |
Expiration termination date: 20120927 Granted publication date: 20031022 |