CN2483395Y

CN2483395Y - Capillary bio-chip device

Info

Publication number: CN2483395Y
Application number: CN 01227862
Authority: CN
Inventors: 郭占军; 赵华; 郭爱芹; 杨焕云
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-06-20
Filing date: 2001-06-20
Publication date: 2002-03-27
Anticipated expiration: 2011-06-20

Abstract

The utility model relates to a capillary biochip device which consists of a transparent capillary provided with a capillary fibre or a capillary fibre rod therein. The capillary fiber or the capillary fibre rod is dotted with stabilized spotting matters, spotting lines or spotting points, as well as corresponding markers, positive and negative control lines, or positive and negative control points thereon. Using the capillary biochip device, the detection, recognition and identification of related genes, pathogens, drugs or receptors, tissues or cells of related diseases can be carried on together just by one step operation. And the capillary biochip device is especially suitable for the detection and identification of cells thus related life information can be got.

Description

The kapillary biochip device

The utility model belongs to life science, relates to a kind of device that gene, protein, medicine or acceptor, tissue or cell are carried out parallel detection, identification, evaluation, particularly a kind of kapillary biochip device.

The prior biological chip is meant high, medium and low density nucleic acid (cDNA, mRNA, PNA) probe, protein (polypeptide, enzyme molecule, antigen, antibody), medicine or the acceptor that is coated on the solid phase carrier, the microarray (Microarray) of cell or tissue.Be to utilize the ability that has special mutual identification between biomacromolecule and with their orderly being arranged on the solid phase carrier substrate (film, sheet glass, silicon chip, ceramics etc.), carry out specific reaction or hybridization simultaneously with sample to be checked and corresponding mark biomolecules, can obtain a large amount of useful life science information by automatic reading equipment.

Gene chip claims the DNA chip again, is a kind of Nucleotide (cDNA, mRNA, PNA probe) microarray of high, middle density.Its preparation method has: the first adopts photoetch and former bit pattern synthesising chemical technology, is solidificated in the nucleic acid probe of a large amount of particular sequences on the carrier substrate in an orderly manner; Its two be with purifying is good the automatic point sample equipment of nucleic acid probe utilization point sample (printing) on carrier substrate; It three is to utilize the photoinduction technology that gene probe is grown at a fibre-optic end; It four is to adopt molecular seal method multi-impression original position fixed point DNA to synthesize on carrier substrate; Thereby constitute the gene chip that stores a large amount of life-information dot matrix.

Protein chip claims peptide chips again, be that protein (polypeptide, enzyme molecule, antigen or antibody) is fixed on the solid phase carrier substrate, utilize protein molecule energy specificity and ligand molecular (antigen or antibody, acceptor) principle of combining, by corresponding tagged molecule, be used for detecting on a large scale antigen, antibody, acceptor or enzyme molecule, polypeptide fragment then.Its making method be with purifying is good protein molecule by different mode point samples (printing) on solid phase carrier; It two is to adopt photoconduction to peptide synthesis technology, will have specific amino acids polypeptide of sequence chain and be solidificated on the carrier substrate in an orderly manner.

The solid phase carrier substrate that biochip is commonly used has: organic and inorganic polymer film, sheet glass, silicon chip, ceramics etc., such carrier substrate is handled through modifying usually, promptly use various activating reagent by chemical reaction various active groups on the carrier substrate surface bond, so that biomolecules covalent attachment with the point sample species, formation has the affinity type carrier substrate of different biologic specificities, it is biochip, be used for fixing and detect various active biomolecule, as protein, polypeptide, enzyme, nucleic acid, antigen, antibody, medicine and acceptor, tissue or cell etc., even on carrier substrate, make up various microstructures (little hole, little ditch, microflute, microtubule, microstructure or devices such as Micropump), be beneficial to the processing of sample and the distribution of reagent.

Above-mentioned biochip complex manufacturing technology often needs special expensive equipment, and respective detection means requirement condition height is unfavorable for conventional the application.

Task of the present utility model just provides a kind of easy and simple to handle quick, and the result is easy to observation and simple for production accurately and reliably, is fit to the kapillary biochip device of producing in batches.

Task of the present utility model is finished like this:

A kind of kapillary biochip device, it is characterized in that: it is made up of transparent capillary, in this kapillary, be provided with capillary fiber silk (bar), go up some line-transect (point), corresponding marker, the yin and yang attribute control line (point) of the multiple different stabilization point sample thing of solid phase covalent attachment simultaneously at capillary fiber silk (bar).

After the utility model adopts above structure, owing to be provided with the kapillary that has capillary fiber silk (bar), only need single stepping, can reach the purpose of parallel detection, identification and the evaluation of gene, antigen or antibody, medicine or acceptor, especially be fit to the detection and the evaluation of cell.The utility model is easy and simple to handle fast, and the result is easy to observe accurately and reliably.Be applicable to detection, identification and evaluation in the life sciences such as disease related gene, pathogenic agent, medicine or acceptor, tissue or cell.Adopt the utility model, once suck sample to be checked and get final product parallel processing, thus the life-information that obtains being correlated with.

Accompanying drawing is a kind of structural representation of the present utility model.

Below in conjunction with accompanying drawing the utility model is described in further detail.

In the accompanying drawing, 1 is that kapillary, 2 is that capillary fiber silk (bar), 3 is that a some line-transect (point), 4 is marker, 5 positive control lines (point), 6 negative control lines (point).

As shown in drawings, the utility model kapillary biochip device is made up of transparent capillary 1, be provided with capillary fiber silk (bar) 2 in this kapillary 1, point has point sample object point line-transect (point) 3, respective markers thing 4, positive control line (point) 5 and the negative control line (point) 6 of stabilization on capillary fiber silk (bar) 2.Outermost layer is a transparent capillary 1, is used to hold capillary fiber silk (bar) 2 and expansion sample.The point sample object point line-transect (point) 3 that contains stabilization on the capillary fiber silk (bar) 2, point sample thing can be any or the arbitrary combinations in the various biomolecules such as dna probe, cDNA probe, PNA probe, mRNA, antigen, antibody (comprising the monoclonal antibody of different loci, anti-people IgM capture antibody, anti-human IgG antibody or two anti-antibodys), drug receptor, polysaccharide lectin, cell or tissue; Two or more point sample object point line-transects (point) 3 are arranged in parallel from bottom to top successively.Respective regions on capillary fiber silk (bar) 2 also contains two or more respective markers things 4, and marker can be any or the arbitrary combination in the markers such as golden mark, iron mark, enzyme labelling, fluorescent mark, chemiluminescent labeling, dye marker and radio-labeling; In addition, the respective regions on capillary fiber silk (bar) 2 also contains two or more positive control line (point) 5 and negative control line (point) 6.In the surveyed area of same test item, the respective markers thing 4, point sample object point line-transect (point) 3, positive control line (point) 5 and the negative control line 6 that contain same test item from bottom to top simultaneously, certain clearance is arranged between four, and its distance is 0.01-1.0mm; The spacing of the surveyed area of different test items is 0.1-2.0mm, is beneficial to the explanation and the detection of surveyed area between each test item; Each surveyed area of different test items is according to from bottom to top order equidistant successively arrangement on capillary fiber silk (bar) 2; Colour developing band or clear spot can be distinguished.

During operation, the lower end of this device is inserted in the sample to be checked, capillarity by transparent capillary and capillary fiber sucks sample in the transparent capillary, sample to be checked can move up and flooded capillary fiber silk (bar) rapidly along transparent capillary this moment, thing while to be checked and point sample thing and marker generation specific reaction or hybridization in the sample to be checked at this moment, observations or washing back sentence read result on interpretation equipment after room temperature 4-60 minute, with colour developing band (point) have that it's too late what, judge detection, identification and the qualification result of two or more things to be checked; There is not colour developing band (point) as the surveyed area of a thing to be checked therein, then this thing to be checked is negative, otherwise positive, also can compare for male thing to be checked with its positive control line (point), according to the colour developing band (point) shade and this thing to be checked is carried out half-quantitative detection; In addition, if each detection zone positive control line (point) colour developing shows that this device is effective, otherwise, show this failure of apparatus.

Claims

1, a kind of kapillary biochip device, it is characterized in that: it is made up of transparent capillary [1], in this kapillary [1], be provided with capillary fiber silk (bar) [2], go up point sample object point line-transect (point) [3], respective markers thing [4], positive control line (point) [5] and the negative control line (point) [6] that point has stabilization at capillary fiber silk (bar) [2].

2, kapillary biochip device according to claim 1, it is characterized in that: above-mentioned marker [4], some line-transect (point) [3], positive control line (point) [5] and negative control line (point) [6] are arranged in order from bottom to top, distance between four is 0.01-1.0mm, and above-mentioned four constitute a kind of surveyed area of test item.

3, kapillary biochip device according to claim 1 and 2 is characterized in that: be provided with the surveyed area of the different test items more than two kinds on above-mentioned kapillary [1], the spacing of each surveyed area is 0.1-2.0mm.