CN2548158Y - Disposable electrochemical sensor for measuring blood lend concentration - Google Patents
Disposable electrochemical sensor for measuring blood lend concentration Download PDFInfo
- Publication number
- CN2548158Y CN2548158Y CN 02264889 CN02264889U CN2548158Y CN 2548158 Y CN2548158 Y CN 2548158Y CN 02264889 CN02264889 CN 02264889 CN 02264889 U CN02264889 U CN 02264889U CN 2548158 Y CN2548158 Y CN 2548158Y
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- electrode
- electrochemical sensor
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- lead
- disposable electrochemical
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Abstract
The utility model is a disposable electrochemical sensor used to test the plumbum concentration in the blood. The prior technology uses atomic absorption spectrometry to test the blood lead, which exists the disadvantages of more testing steps, long testing time, high instrument cost, worse reproducibility and etc.. The utility model uses a round working electrode, an arc shaped antipode and an arc shaped reference electrode, which are printed by a printing plate, and the lead thereof is connected with an analysis and test instrument to test the plumbum concentration in the blood. Conductive silver pulp is arranged at the bottom layer of the working electrode, the antipode and the lead, fifty percent of the surface layer area is provided with Ag, the other area of the surface layer is provided with AgCl, the lead part is covered by insulation layer. The utility model sensor is less in manufacture cost, disposable in product using, stable in detection test, convenient in detection operation.
Description
Technical field
The utility model is a kind of structure that is used to measure the disposable electrochemical sensor of blood lead concentration
Background technology
Along with the high speed development of modern industry and traffic, the environment lead contamination is on the rise, and it is human to have become influence, especially children's health important social concern.Studies confirm that Pb-B surpasses 100 μ g/L and will influence growth of children, particularly damage children's neurodevelopment, influence children's intelligence and behavior.Because the physiology aspect, the preschool child is to the special susceptible of the neurotoxicity of lead, and therefore, many developed countries such as the U.S. all force to carry out the examination of 6 years old following lead levels by legislation.The Pb-B average of discovering children in Shanghai area is up to 96 μ g/L, and the ratio that wherein is greater than or equal to present internationally recognized children ' s lead poisoning diagnostic criteria is 37.8%.This average Pb-B is far above 36 μ g/L of present U.S. children, and the lead contamination problem that as seen solves in the area surroundings of Shanghai is very urgent.Present blood lead assay method mainly is to adopt GFAAS (graphite furnace atomic absorption spectrometry) to measure, and this method need be used large-sized analytic instrument---Atomic Absorption Spectrometer, and determination step is many, the time is long, cost is higher.Therefore developing quick, easy, sensitive blood lead detection means seems very necessary.
Summary of the invention
The purpose of this utility model is the disposable electrochemical sensor of a kind of cost of design mensuration blood lead concentration low, easy to prepare.
The purpose of this utility model is a kind of disposable electrochemical sensor that detects mensuration blood lead concentration accurate, easy to use of design.
Sensor of the present utility model is by being printed on working electrode on the printed panel, the utmost point, contrast electrode being formed, working electrode is printed on the circle of diameter 1.5-2.0mm on the printed panel, to the utmost point is the circular arc that is printed on the diameter 3.0-4.0mm of working electrode periphery, the corresponding 270 degree circumferences of arc length, contrast electrode also is that the diameter in the working electrode periphery is the circular arc of 3.0-4.0mm, the corresponding 90 degree circumferences of arc length.The extension line of three electrodes is drawn along the printed panel length direction, and the extension line remainder is all covered by the rectangle insulation course except that the extension line end.Working electrode and extension line thereof and the utmost point and extension line bottom thereof printed by conductive silver paste, the top layer is printed by conductive carbon paste, contrast electrode and extension line bottom thereof are to be to print by respectively accounting for 50% AgCl powder and conductive silver paste by conductive silver paste, top layer, and insulation course is dielectric ink such as BYR9841 board dielectric ink (going up Hypon silver company).The extension line end is connected with electrochemical analyser, as stripping voltamograph etc.
The printed panel of the sensor is long 25-30mm, wide 15-20mm, and the rectangular parallelepiped of thick 15-20 μ m, very small and exquisite, material can be made with PET or PVC substrate.
The electrode of on printed panel, making and draw live width 1.0-1.5mm.
The extension line of electrode is drawn along the printed panel length direction, and its lead portion is by long 11-15mm, and the rectangle insulation course of wide 12-16mm hides multiple.
Electrode outlet line length all is 12-16mm.
The electrode outlet line end is exposed, is convenient to be connected with analytical instrument such as stripping voltamograph etc.
Sensor of the present utility model can be connected with three electrode tie-in lines of multifunction electric chemical analyzer (or stripping voltamograph), bleed the final proof product when being added drop-wise to the front end dashed region of this sensor when one, the part of sample solution and three-electrode system will form a small electrochemical cell, thereby can use stripping voltammetry to realize the plumbous detection of trace.In order to improve the sensitivity of electrode, can before each mensuration, plate mercury to working electrode (WE, carbon ink electrode) surface in advance and handle.Total determination step is as follows:
(1) adopt potentiostatic method or potential step method to WE plating mercury film, the condition of potentiostatic method is :-0.904V (v.s. saturated calomel electrode), three-electrode system reach among the 1.2mol/L HCl and electroplated 5 minutes at 13.8mg/L HgCl; The condition of potential step method is 13.8mg/L HgCl, and among the 1.3mol/L HCl, initial-0.3V (v.s.saturated mercurous chloride electrode), each minute falls-0.1V later on.Electroplated 6 minutes, voltage is-0.8V during end.
(2) use stripping voltammetry to blank solution and the scanning of standard lead solution: electroplated 120 seconds under-0.7v condition earlier, after static 15 seconds, reverse scan is to-0.2v.Wherein anti-phase sweep velocity 0.02V/s, amp=0.05V, pw=0.05s.Near-0.6V, obtain one group of good standard solution stripping peak.Good in 39ng/mL to 165ng/Ml scope internal linear, see Fig. 2.
Design plate-making of the present utility model can adopt screen printing technique to produce required disposable electrochemical sensor on a large scale.In electrochemical sensor design in the past, working electrode is gold electrode or the carbon electrode that can reuse repeatedly usually.This reusable electrode complex manufacturing process, cost is higher, simultaneously may cause in various degree pollution to electrode surface owing to measure process each time, so that all needed kind electrode is carried out surface treatment before the mensuration of carrying out subsequently.This disposal skill is very big to layman's difficulty, the stability and the reappearance of sensor between therefore can't guaranteeing to measure for several times.Also increased the step of measuring in addition.Sensor by adopting the utility model to provide is provided the implication of " disposable ", can be on a large scale, many almost completely the same sensors of batch making at low cost, every sensor can abandon after having measured a sample, and uses the next sample of a new sensor determination instead.Like this, make that the used sensor sheet surface state of each working sample is approaching, thereby guarantee the accuracy and the reappearance of mensuration.And the utility model cost of products is not high, and is easy to use.
Description of drawings
Fig. 1 is the utility model sensor construction figure.
Fig. 2 is the standard lead solution figure that the utility model sensor uses stripping voltammetry.
Fig. 3 is the utility model working sensor electrode, to the utmost point and extension line bottom thereof, surface structure synoptic diagram.
Fig. 4 is the utility model sensor contrast electrode and extension line bottom thereof, surface structure synoptic diagram.
1 is working electrode among the above-mentioned figure, the 2nd, and to the utmost point, the 3rd, contrast electrode, 4,5,6 is respectively the former electrodes extension line, 7,8,9 is respectively former electrodes extension line end, the 10th, and conductive silver slurry layer, the 11st, conductive carbon paste layer, the 12nd, Ag/Acl layer, the 13rd, printing plate substrate, the 14th, insulation course.
The manufacturing process of this disposable sensor of embodiment is illustrated by following example:
Example 1:
By the screen printing plate that designs and produces shown in Figure 1.It is that 20 micron thickness, length are that 30mm, width are the PET film surface of 20mm that whole sensor is printed on thickness.Electrode 1,2,3 is respectively working electrode, to the utmost point and contrast electrode.The diameter of working electrode 1 (circular reaction zone) is 2.0mm.1 to draw lead (being insulated layer cover part and top bared end) length be 16mm, width is 1.5mm.Working electrode 1 and draw the lead bottom and all use conductive silver paste (BY9902, last Hypon silver company) to print, its top layer is by conductive carbon paste (BY9710A, last Hypon silver company) printing.Diameter to the utmost point 2 (orthodrome district) is 3.5mm, the corresponding 270 degree circumferences of arc length, and width is 1.5mm.2 to draw lead (being insulated layer cover part and top bared end) length be 16mm, width is 1.5mm.The utmost point 2 is drawn the lead bottom all use conductive silver paste (BY9902, the silver-colored company of last Hypon) to print, its top layer is printed by conductive carbon paste (BY9710A, the silver-colored company of last Hypon).The diameter of contrast electrode (roundlet arc district) is 3.5mm, the corresponding 90 degree circumferences of arc length, and width is 1.5mm.The extension line of contrast electrode (being insulated layer cover part and top bared end) length is 16mm, and width is 1.5mm.Contrast electrode and draw the lead bottom and all use conductive silver paste (BY9902; the silver-colored company of last Hypon) prints; its top layer is evenly printed the mixing back with 50% conductive silver paste (BY9902, the silver-colored company of last Hypon) by 50% AgCl powder (granularity 250 orders, Shanghai chemical reagents corporation).Insulation course is that length is 15mm, and width is the rectangle of 16mm, and (BYR9841, the silver-colored company of last Hypon) prints by dielectric ink.
Example 2:
Utilize the preparation condition of example 1, can prepare the sensor of different size, concrete size is as follows:
Printed panel: length * wide * thick=25 * 15 (mm) * 15 (μ m)
Working electrode diameter: 1.5mm;
To polar diameter: 3.0mm;
Contrast electrode diameter: 3.0mm;
Each electrode draw line length 12mm, wide 1.0mm;
Insulation course: length * wide=11 * 12 (mm)
Adopt disposable silk-screen three electrode sensors of the present utility model, can greatly improve repeatedly measure heavy linear, reduce analytical error.And owing to can make in large quantity, the cost of single measurement is also low more than the atomic absorption method that uses at present.Similar design also can be used for the mensuration of Pb in environmental water sample or the drug sample.
Claims (6)
1, a kind of disposable electrochemical sensor of measuring blood lead concentration, by the working electrode on the printed panel, the utmost point, contrast electrode are formed, it is characterized in that working electrode (1) is that diameter is the circle of 1.5-2.0mm, to the utmost point (2) is that diameter is the circular arc of 3.0-4.0mm, the corresponding 270 degree circumferences of arc length; Contrast electrode (3) is that diameter is the circular arc of 3.0-4.0mm, the corresponding 90 degree circumferences of arc length, and the extension line of three electrodes is respectively (4), (5), (6), insulation course (14) is a rectangle; Working electrode and extension line thereof, be conductive silver slurry layer to the utmost point and extension line bottom thereof, the top layer is a conductive carbon paste layer, contrast electrode and extension line bottom thereof are conductive silver slurry layers, the top layer is respectively to account for 50% AgCl powder and conductive silver slurry layer, insulation course is a dielectric ink, draws line end and connects with electrochemical analyser.
2, the disposable electrochemical sensor of mensuration blood lead concentration according to claim 1, the printed panel that it is characterized in that sensor are the rectangular parallelepipeds of long 25-30mm, wide 15-20mm, thick 15-20 μ m.
3, the disposable electrochemical sensor of mensuration blood lead concentration according to claim 1 is characterized in that the width of electrode (1), (2), (3) and extension line (4), (5), (6) is 1-1.5mm.
4, the disposable electrochemical sensor of mensuration blood lead concentration according to claim 1 is characterized in that insulation course is the rectangle of long 11-15mm, wide 12-16mm.
5, want the disposable electrochemical sensor of 1 described blood lead concentration according to right, it is characterized in that electrode (1), (2), (3) length of lead-out wire all are 12-16mm.
6, the disposable electrochemical sensor of blood lead concentration according to claim 1 is characterized in that the extension line end connects the stripping voltamograph.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02264889 CN2548158Y (en) | 2002-06-20 | 2002-06-20 | Disposable electrochemical sensor for measuring blood lend concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02264889 CN2548158Y (en) | 2002-06-20 | 2002-06-20 | Disposable electrochemical sensor for measuring blood lend concentration |
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| CN2548158Y true CN2548158Y (en) | 2003-04-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 02264889 Expired - Fee Related CN2548158Y (en) | 2002-06-20 | 2002-06-20 | Disposable electrochemical sensor for measuring blood lend concentration |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102135518A (en) * | 2011-01-07 | 2011-07-27 | 上海交通大学 | Detection electrode of trace lead in drinking water and preparation method thereof |
| CN102243209A (en) * | 2010-05-14 | 2011-11-16 | 湖南友能高新技术有限公司 | Portable heavy metal chrome sensor, preparation process and detection method thereof |
| CN102253086A (en) * | 2010-03-24 | 2011-11-23 | 孙一慧 | Seonsor and method for detecting integrated electrode of analyte in fluid |
| CN102576004A (en) * | 2010-09-27 | 2012-07-11 | 松下电器产业株式会社 | A method for quantifying a chemical substance with substitutional stripping volammetry and a sensor chip used therefor |
| CN103123333A (en) * | 2012-12-31 | 2013-05-29 | 北京师范大学 | Method for rapidly detecting lead based on three-electrode sensor and differential pulse voltammetry |
| CN103149265A (en) * | 2012-12-31 | 2013-06-12 | 北京师范大学 | Quick copper-testing method based on linear sweep voltammetry and three-electrode sensor |
| CN103913501A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on differential pulse voltammetry and three-electrode sensor |
| CN103913502A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on square-wave stripping voltammetry and three-electrode sensor |
| CN104833711A (en) * | 2015-03-09 | 2015-08-12 | 上海伯顿医疗设备有限公司 | Blood lead analysis instrument and blood lead measuring method therewith |
| CN104950021A (en) * | 2014-03-28 | 2015-09-30 | 无锡市申瑞生物制品有限公司 | Disposable type silk screen printing sensor for detection of blood lead and manufacture method thereof |
| CN106841347A (en) * | 2016-12-30 | 2017-06-13 | 北京农业信息技术研究中心 | The online test method in situ of reduced glutathione in plant |
| CN107917942A (en) * | 2016-10-11 | 2018-04-17 | 广州好芝生物科技有限公司 | A kind of electrode system and strip and instrument containing the electrode system |
-
2002
- 2002-06-20 CN CN 02264889 patent/CN2548158Y/en not_active Expired - Fee Related
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102253086A (en) * | 2010-03-24 | 2011-11-23 | 孙一慧 | Seonsor and method for detecting integrated electrode of analyte in fluid |
| CN102243209A (en) * | 2010-05-14 | 2011-11-16 | 湖南友能高新技术有限公司 | Portable heavy metal chrome sensor, preparation process and detection method thereof |
| CN102576004A (en) * | 2010-09-27 | 2012-07-11 | 松下电器产业株式会社 | A method for quantifying a chemical substance with substitutional stripping volammetry and a sensor chip used therefor |
| CN102135518A (en) * | 2011-01-07 | 2011-07-27 | 上海交通大学 | Detection electrode of trace lead in drinking water and preparation method thereof |
| CN102135518B (en) * | 2011-01-07 | 2013-05-01 | 上海交通大学 | Detection electrode of trace lead in drinking water and preparation method thereof |
| CN103913501B (en) * | 2012-12-31 | 2018-01-26 | 北京师范大学 | The method for quickly determining copper based on Differential Pulse Voltammetry and three electrode sensors |
| CN103123333A (en) * | 2012-12-31 | 2013-05-29 | 北京师范大学 | Method for rapidly detecting lead based on three-electrode sensor and differential pulse voltammetry |
| CN103913501A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on differential pulse voltammetry and three-electrode sensor |
| CN103913502A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on square-wave stripping voltammetry and three-electrode sensor |
| CN103149265A (en) * | 2012-12-31 | 2013-06-12 | 北京师范大学 | Quick copper-testing method based on linear sweep voltammetry and three-electrode sensor |
| CN103913502B (en) * | 2012-12-31 | 2017-12-26 | 北京师范大学 | The method for quickly determining copper based on square wave stripping voltammetry and three electrode sensors |
| CN103123333B (en) * | 2012-12-31 | 2016-03-02 | 北京师范大学 | Based on the method for three electrode sensors and Differential Pulse Voltammetry Fast Measurement lead |
| CN103149265B (en) * | 2012-12-31 | 2016-03-02 | 北京师范大学 | Based on the method for linear sweep voltammetry and three electrode sensor Fast Measurement copper |
| CN104950021A (en) * | 2014-03-28 | 2015-09-30 | 无锡市申瑞生物制品有限公司 | Disposable type silk screen printing sensor for detection of blood lead and manufacture method thereof |
| CN104950021B (en) * | 2014-03-28 | 2018-04-17 | 无锡市申瑞生物制品有限公司 | A kind of deserted silk-screen printing sensor for blood lead analysis and preparation method thereof |
| CN104833711A (en) * | 2015-03-09 | 2015-08-12 | 上海伯顿医疗设备有限公司 | Blood lead analysis instrument and blood lead measuring method therewith |
| CN104833711B (en) * | 2015-03-09 | 2019-06-25 | 上海伯顿医疗设备有限公司 | A kind of blood lead analyzer and its measurement blood lead method |
| CN107917942A (en) * | 2016-10-11 | 2018-04-17 | 广州好芝生物科技有限公司 | A kind of electrode system and strip and instrument containing the electrode system |
| CN106841347A (en) * | 2016-12-30 | 2017-06-13 | 北京农业信息技术研究中心 | The online test method in situ of reduced glutathione in plant |
| CN106841347B (en) * | 2016-12-30 | 2019-04-02 | 北京农业信息技术研究中心 | The online test method in situ of reduced glutathione in plant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |