CN2505830Y - Improved nematode separator - Google Patents
Improved nematode separator Download PDFInfo
- Publication number
- CN2505830Y CN2505830Y CN 01272572 CN01272572U CN2505830Y CN 2505830 Y CN2505830 Y CN 2505830Y CN 01272572 CN01272572 CN 01272572 CN 01272572 U CN01272572 U CN 01272572U CN 2505830 Y CN2505830 Y CN 2505830Y
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- nematode
- sample containing
- containing bucket
- extension
- rubber tube
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Abstract
The utility model relates to an improved nematode separator which is used for separating the active nematode in plant tissue, soil, plant growth medium, animal tissue, etc. The improved nematode separator comprises a sample holding funnel, wherein, a lower mouth of the sample holding funnel is connected with a rubber tube, a water stop clip is arranged at an outlet of the rubber tube, an outer rim of an upper mouth of the sample holding funnel is integrally connected with an extending section upwards, an inner diameter of the extending section matches with the outer diameter of a sieve disc, which is arranged in the extending section. The utility model has the advantages of high separation rate of nematode, large separation quantity, good stability, elegant appearance and small occupied aera, hence a nematode separator of convenience, rapidness, high efficiency and economy.
Description
Technical field
The utility model relates to a kind of nematode separation vessel that is used for the activity nematode of isolating plant tissues, soil, plant growth medium and animal tissue etc.
Background technology
Existing nematode separator technology is that Baermann puts forward at first.Diameter commonly used is 8-10 centimetre a glass funnel, filler tube connects one section emulsion tube, switching with tongs (spring clamp) control sebific duct, funnel is placed in the annulus of brandreth or homemade wooden filtration stand, inject about 2/3 clear water in it, get the soil sample 100-200 gram that contains nematode, or the vegetable material (as root system) that contains nematode is cut into about 0.5-1.0 centimetre segment, encase soil sample or root sample with four layers of gauze or two floor height level toilet papers (cotton), put into the funnel that is filled with water gently, make water cover, soak into, as water shortage, add water gently, prevent to go out mud, through 10-12 hour, be connected on empty beaker under the sebific duct of funnel, unclamp tongs gently, flow out water, contain a large amount of nematodes from funnel.Close sebific duct, replenish clear water gently, can collect nematode once more to funnel.But this nematode separation vessel exists that the nematode separation rate is low, fractional dose is little, and stability is bad, lacks defectives such as one-piece construction.
Summary of the invention
The purpose of this utility model is exactly in order to address the above problem, and provides a kind of nematode separation rate height, fractional dose is big and the modified nematode separation vessel of good stability.
The scheme that the utility model solves:
A kind of modified nematode separation vessel, it includes sample containing bucket, the sample containing bucket end opening is connected with rubber tube, the rubber tube exit is provided with tongs, the outer rim suitable for reading that the it is characterized in that sample containing bucket integral body that makes progress is connected with one section extension, the internal diameter of this extension and screen tray external diameter match, and screen tray just is arranged in this extension.
Separation vessel of the present utility model adopts the sample containing bucket by mesh screen, emulsion tube, tongs and a moulded section to constitute, and about 18 centimetres of mesh screen diameter has guaranteed plant tissue or the above soil sample of 400 grams that disposable separation 200 grams are above.The special material that the sample containing bucket inside surface adds has improved its smooth finish most possibly, and the design on funnel part 60 degree inclined-planes guarantees not hold from nematode rapid subsidence to the emulsion tube that sample overflows.The utility model can be widely used in the activity nematode in isolating plant tissues, soil, plant growth medium and the animal tissue etc.The detection of timber, wooden packing nematode of being used to pass in and out is convenient especially, efficient; Be used to import and export the detection by quantitative that nursery stock, potted landscape, flowers etc. go up nematode, not only easy and simple to handle, and comparability arranged most, the characteristics that it is light, sturdy and durable and easy to use, send department for plant quarantine such as the airport of testing conditions shortcoming, harbour, gloomy inspection stations, fast detecting nematodiasis provides convenience.The utlity model has advantage elegant in appearance and that occupation of land is little, is a kind of convenient, fast, efficient and economic nematode separation vessel.Pine wood nematode is an example in the timber to separate, and the utility model can be isolated the pathogenic nematode more than 70% in the 100 gram diseased woods in 24 hours, collect the parting liquid that emulsion tube is not held, and just can obtain purer nematode parting liquid.Use φ 18cm/10 purpose mesh screen in the utility model detachment process, improved separation rate, guaranteed the sample that disposable separation is relatively large, improved the separation efficiency of nematode simultaneously biglyyer.The utility model cost is low, the separation vessel that it is integrated, when separating nematode in the winter time, can pass in and out the heat and moisture preserving incubator easily, can be widely used in the activity nematode that exists in isolating plant tissues, the soil, thereby be plant quarantine department, colleges and universities, agri-scientific research institute/the be used for good equipment of investigation and research nematode.The utility model main body adopts pressing mold of novel synthetic chemical materials to form, and is attractive in appearance, generous and sturdy and durable, design service life 20 years.The utility model top is detachable stainless steel mesh screen, make finish clean behind the mask work very convenient.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
As Fig. 1, the utility model includes sample containing bucket 1, sample containing bucket 1 end opening is connected with rubber tube 2, rubber tube 2 exits are provided with tongs 3, characteristics of the present utility model are, the outer rim suitable for reading of sample containing bucket 1 integral body that makes progress is connected with one section extension 4, and the internal diameter of this extension 4 and screen tray 5 external diameters match, and screen tray 5 just is arranged in this extension 4.
Sample containing bucket 1 diameter suitable for reading of the present utility model is 15-18 centimetre, is convenient to improve the sample amount of containing.Sample containing bucket 1 bottom outer wall integral body is connected with pedestal 6 downwards, and this pedestal 6 highly is 20-25 centimetre, and it guarantees the steadiness of funnel 1 on the one hand, guarantees again that on the other hand rubber tube 2 stretches out pedestal 6 by certain downward gradient.Simultaneously,, just make the utility model form an one-piece construction, be convenient to put into the heat and moisture preserving incubator, satisfy the temperature requirement that separates the nematode defined winter because the utility model changes the long brandreth of traditional sample containing bucket the pedestal 6 of certain altitude into.
The utility model using method is as follows:
Nematode in the isolating plant tissues: nematode filter paper (or double-deck napkin paper, thieving paper etc.) is tiled in the screen tray 5, uses the low amounts of water wet edge, make filter paper be adjacent to screen tray 5.Plant tissue chopping is placed on the filter paper in the screen tray 5 to suitable size, adds water to the position (gently pushing rubber tube 2 with have gentle hands, wherein the bubble of can rushing) of sample levels from screen tray 5 outer rims with wash bottle.
Separate the nematode in soil or other medium: sample earlier with behind one deck napkin paper parcel, is placed on the filter paper of screen tray 5, and the same method is separated nematode.
Be used for hatching cyst or egg capsule nematode: with cyst or contain the soil of cyst, the plant tissue that contains egg capsule is put filter paper in the screen tray 5, add water and touch the sample bottom.On sample, cover double-deck moistening gauze, husky cloth edge suitably is pressed under the filter paper, and contacts, regularly add water and keep water surface balance with water.
Nematode is a happiness hydrodynamic(al) thing, meets water and just can swim out of in soil or plant tissue, simultaneously because terrestrial attraction is the weight of nematode self, just can sink down into interior the concentrating of pipe of funnel 1.
Claims (4)
1, a kind of modified nematode separation vessel, it includes sample containing bucket, the sample containing bucket end opening is connected with rubber tube, the rubber tube exit is provided with tongs, the outer rim suitable for reading that the it is characterized in that sample containing bucket integral body that makes progress is connected with one section extension, the internal diameter of this extension and screen tray external diameter match, and screen tray just is arranged in this extension.
2, by the described modified nematode of claim 1 separation vessel, it is characterized in that described screen tray is made up of the stainless steel mesh of stainless steel cylinder and bottom surface, this cylindrical shell is the awl tubular, correspondingly sample containing bucket also taper of extension suitable for reading for matching, the upper limb of screen tray stainless steel cylinder can be provided with flanging, and it is erected on the sample containing bucket extension suitable for reading.
3, by the described modified nematode of claim 1 separation vessel, it is characterized in that sample containing bucket diameter suitable for reading is 15-18 centimetre.
4, by the described modified nematode of claim 1 separation vessel, it is characterized in that sample containing bucket bottom outer wall integral body is connected with pedestal downwards, this base height is 20-25 centimetre, and rubber tube stretches out pedestal by certain downward gradient.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 01272572 CN2505830Y (en) | 2001-12-03 | 2001-12-03 | Improved nematode separator |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 01272572 CN2505830Y (en) | 2001-12-03 | 2001-12-03 | Improved nematode separator |
Publications (1)
Publication Number | Publication Date |
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CN2505830Y true CN2505830Y (en) | 2002-08-14 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN 01272572 Expired - Fee Related CN2505830Y (en) | 2001-12-03 | 2001-12-03 | Improved nematode separator |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100363729C (en) * | 2005-07-21 | 2008-01-23 | 云南农业大学 | Portable fast separation device of pine material nematode and its separation method |
CN100496748C (en) * | 2005-01-05 | 2009-06-10 | 中国科学院东北地理与农业生态研究所 | Soil animals separator |
CN102628760A (en) * | 2012-03-31 | 2012-08-08 | 江苏徐淮地区淮阴农业科学研究所 | Cup-shaped multi-stage nematode separator |
CN102812875A (en) * | 2012-09-11 | 2012-12-12 | 鹤壁佳多科工贸有限责任公司 | Intelligent device for collecting wheat midge from soil and application method thereof |
CN104020034A (en) * | 2013-03-01 | 2014-09-03 | 中国疾病预防控制中心寄生虫病预防控制所 | Method for separating hookworm larvae in soil |
CN104429736A (en) * | 2014-11-13 | 2015-03-25 | 河南省农业科学院植物保护研究所 | Wheat blossom midge soil removal, rinsing and sorting device and use method thereof |
CN106035238A (en) * | 2016-05-30 | 2016-10-26 | 中国科学院东北地理与农业生态研究所 | Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes |
CN108480195A (en) * | 2018-05-18 | 2018-09-04 | 中国科学院东北地理与农业生态研究所 | A kind of Soil sediment automatic splinter screening device for screening and its application method |
CN110068494A (en) * | 2019-04-28 | 2019-07-30 | 石河子大学 | One group of soil nematodes extraction element and extracting method |
-
2001
- 2001-12-03 CN CN 01272572 patent/CN2505830Y/en not_active Expired - Fee Related
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100496748C (en) * | 2005-01-05 | 2009-06-10 | 中国科学院东北地理与农业生态研究所 | Soil animals separator |
CN100363729C (en) * | 2005-07-21 | 2008-01-23 | 云南农业大学 | Portable fast separation device of pine material nematode and its separation method |
CN102628760A (en) * | 2012-03-31 | 2012-08-08 | 江苏徐淮地区淮阴农业科学研究所 | Cup-shaped multi-stage nematode separator |
CN102628760B (en) * | 2012-03-31 | 2014-04-09 | 江苏徐淮地区淮阴农业科学研究所 | Cup-shaped multi-stage nematode separator |
CN102812875A (en) * | 2012-09-11 | 2012-12-12 | 鹤壁佳多科工贸有限责任公司 | Intelligent device for collecting wheat midge from soil and application method thereof |
CN102812875B (en) * | 2012-09-11 | 2013-10-30 | 鹤壁佳多科工贸有限责任公司 | Intelligent device for collecting wheat midge from soil and application method thereof |
CN104020034A (en) * | 2013-03-01 | 2014-09-03 | 中国疾病预防控制中心寄生虫病预防控制所 | Method for separating hookworm larvae in soil |
CN104429736A (en) * | 2014-11-13 | 2015-03-25 | 河南省农业科学院植物保护研究所 | Wheat blossom midge soil removal, rinsing and sorting device and use method thereof |
CN106035238A (en) * | 2016-05-30 | 2016-10-26 | 中国科学院东北地理与农业生态研究所 | Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes |
CN108480195A (en) * | 2018-05-18 | 2018-09-04 | 中国科学院东北地理与农业生态研究所 | A kind of Soil sediment automatic splinter screening device for screening and its application method |
CN108480195B (en) * | 2018-05-18 | 2023-10-03 | 中国科学院东北地理与农业生态研究所 | Automatic soil sediment screening device and application method thereof |
CN110068494A (en) * | 2019-04-28 | 2019-07-30 | 石河子大学 | One group of soil nematodes extraction element and extracting method |
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