CN2497313Y

CN2497313Y - Protein chip

Info

Publication number: CN2497313Y
Application number: CN 01253423
Authority: CN
Inventors: 胡赓熙
Original assignee: Shanghai Health Digit Ltd
Current assignee: Shanghai Health Digit Ltd
Priority date: 2001-08-31
Filing date: 2001-08-31
Publication date: 2002-06-26
Anticipated expiration: 2011-08-31

Abstract

The utility model relates to an albumen chip. Most of the prior detection technologies are single index detection with small information quantity and localization. The albumen chip provided by the utility model includes a box cover (1) and a box body (5). The utility model is characterized in that the box cover (1) is a porous plate; the box body (5) consists of a membrane (2) with a plurality of albumen lattices and plastered on a gasket, a porous gasket (3) with dual-surface stickiness and a porous plate (4) stuck below the gasket. By adopting the albumen chip with the structure, the utility model has the advantages of stable structure, convenient application, high sensitivity, etc. The utility model can be applied to multi-index parallel detection.

Description

A kind of protein chip

Technical field

The utility model belongs to external clinical diagnosis detection technique field, exactly, belongs to a kind of protein chip that is suitable for the multiple disease of many indexs parallel detection.

Background technology

If many malignant diseases such as tumour, pernicious infectious disease etc. are not as good as diagnosis and treatment early, its mortality ratio then increases greatly, and consequence is hardly imaginable.Existing methods for the diagnosis of diseases comprises chemical diagnosis method and physical diagnostic method.Because physical method can not be made diagnosis to early stage disease, and need a large amount of instrument, equipment and necessarily required environment, the expense costliness therefore manyly promptly detects disease related protein in patient's body or the content of small-molecule substance diagnoses the illness with chemical method.

But, detect the chemical method of disease at present, as ELISA, labelled with radioisotope, golden mark, fluorescence, chemiluminescence, time-resolved fluorescence etc., be that single index detects mostly, quantity of information is few, and all have limitation, as markers step complexity, instrument and reagent costliness, operation is loaded down with trivial details, sensitivity is low, easy pollution etc.And it is non-specificity index that various diseases detects the index great majority, not only a plurality of disease related protein content significantly raise simultaneously in same disease, and same disease related protein content all can raise in multiple disease, and this situation causes the difficulty in the medical diagnosis on disease, causes mistaken diagnosis.Detection such as malignant tumour, existing tumor-marker albumen all is non-specificity index, even the primary carcinoma of liver index AFP (alpha-fetoprotein) that generally acknowledges has only about 70% the recall rate of liver cancer, and AFP also has obviously in patients serums such as some carcinoma of testis, teratoma, cancer of the stomach, cancer of pancreas and increases.As cancer of pancreas: tumor markers CA19-9 recall rate is about 60%, adds that CA125, CA50 and DU-PAN-then can mention recall rate more than 90%.Lung cancer for another example: single with CEA to the existing higher specificity of pulmonary cancer diagnosis, but as add NSE, sialic acid, CEA, SCC then specificity be higher.Therefore, just be necessary to propose multiple markers in detecting theory.

For this reason, people urgently expect to adopt the joint-detection of kinds of tumors mark, detect weak point to overcome above-mentioned single index by complementation.

Summary of the invention

The purpose of this utility model just provides a kind of protein chip that is used for many indexs parallel detection disease.In order to achieve the above object, the utility model has designed a kind of protein chip, and it is made up of lid, box body; Wherein, described lid is the plate that porous is arranged, described box body comprise be pasted on pad top have a plurality of albumen dot matrix film, biadhesive porose pad and be pasted on the abacus of pad below.

The utility model adopts the film with a plurality of albumen dot matrix, and each protein site lattice point has multiple disease related protein mark, and each dot matrix is corresponding to each hole on the plate.This film can be cellulose acetate membrane, nitrocellulose filter, nylon membrane, biochip bases such as polypropylene screen.

The porose pad that the utility model uses has biadhesive, and its effect is not only can be pasted on the abacus of below, and the film with significant albumen dot matrix can be pasted on the top of pad, prevents that film from sliding.

The porose plate that is pasted on the pad below that the utility model adopts has the plane that projects upwards; pad is to be pasted on the outstanding plane of bottom plastic plate; the area of pad is identical with the outstanding area of plane; the outstanding plane and the volume of pad and film and the inner chamber of lid match, and can play the effect of diaphragm.The position of each the albumen dot matrix on the film helps operator's application of sample corresponding to each hole of lid and box body.

In addition, as further improvement of the utility model, it reaches at lid, pad sign of filling on the outstanding plane, help the locality that the operator distinguishes protein chip, and be marked with numeration number at lid and box body plate, count when helping operator's application of sample.The demarcation strip in each hole can protrude in this plastic plate above and below on lid, can effectively each the albumen dot matrix on the film be separated, and enough height are arranged, and helps preventing cross pollution between the sample.

In sum, advantage of the present utility model is: easy to use, simple in structure, be convenient to extensive detection, and once can detect many people, because the parallel detection of many indexs makes this instrument be greatly improved to the sensitivity and the specificity of medical diagnosis on disease.

Description of drawings

Fig. 1 is the structural representation of the utility model protein chip.

Fig. 2 is the decomposition diagram of protein chip among Fig. 1.

Fig. 3 is the vertical view of box body lower panel face among Fig. 2.

Embodiment

Further set forth the utility model with specific embodiment below, but do not limit scope of the present utility model.

By Fig. 1, shown in Figure 2, a kind of protein chip that the utility model provides comprises lid 1 and box body 5 two parts.Wherein, the lid of described protein chip is the plastic plate 1 that porous is arranged, described hole can be multiple shapes such as square, circle, the quantity in the hole that the utility model uses is 48, the demarcation strip in each hole protrudes in this plastic plate above and below, and be close on the diaphragm 2, prevent cross pollution between the sample when helping dripping sample.

The box body 5 of a kind of protein chip that the utility model provides comprise be pasted on pad top have 48 significant albumen dot matrix nitrocellulose filter 2, biadhesive 48 hole sheet rubbers 3 and be pasted on 48 hole plastic plates 4 of pad below.Wherein, described plastic plate 4 has outstanding plane, and the area on plane is slightly less than this plastic plate 4, so that match with above-mentioned lid 1; Be stained with 48 hole sheet rubbers 3 of biadhesive on the outstanding plane, be stained with nitrocellulose filter 2 on the sheet rubber 3, film 2 is fixed on the box body 5, be convenient to operation with 48 tumor-marker albumen dot matrix.The outstanding plane and the volume of pad 3 and film 2 and the inner chamber of lid 1 match, and each albumen dot matrix helps the parallel detection of many indexs corresponding to each hole on lid 1 and the box body 5 on the film 2.By Fig. 2, shown in Figure 3, the lower right corner of described lid 1, film 2 and sheet rubber 3 becomes blunt shape, as the tick lables 6 of this protein chip, and is marked with numeration numbers 7 at lid 1 and box body plate 4.

Claims

1, a kind of protein chip, comprise lid (1) and box body (5), it is characterized in that: described lid (1) is the plate that porous is arranged, described box body (5) comprise be pasted on pad (3) top have a plurality of albumen dot matrix film (2), biadhesive porose pad (3) and be pasted on abacus (4) below the pad (3).

2, a kind of protein chip according to claim 1 is characterized in that: the hole of described lid (1) can be multiple shapes such as square, circle.

3, a kind of protein chip according to claim 1, it is characterized in that: the plate (4) of described box body (5) below has outstanding plane, paste pad (3) on it, the area of pad (3) is identical with outstanding plane, and the outstanding plane and the volume of pad (3) and film (2) and the inner chamber of lid (1) are wanted to coincide.

4, a kind of protein chip according to claim 1 is characterized in that: each the albumen dot matrix on the film (2) on the described box body (5) is corresponding with the position in each hole of lid (1) and box body (5).

5, a kind of protein chip according to claim 1 is characterized in that: the film (2) on the described box body (5) can be cellulose acetate membrane, nitrocellulose filter, nylon membrane, biochip bases such as polypropylene screen.

6, a kind of protein chip according to claim 1 is characterized in that: described box body (5) is gone up porose pad (3) and is had biadhesive.

7, a kind of protein chip according to claim 1, it is characterized in that: described lid (1), pad (3) and plate (4) are gone up the sign (6) that a display chip locality is arranged in the outstanding plane, and are marked with numeration number (7) at the plate (4) of lid (1) and box body (5).