CN2486557Y

CN2486557Y - Cell cultivator

Info

Publication number: CN2486557Y
Application number: CN 01201586
Authority: CN
Inventors: 杨俊林; 王身国; 贝建中; 杨建�
Original assignee: Institute of Chemistry CAS
Current assignee: Institute of Chemistry CAS
Priority date: 2001-01-19
Filing date: 2001-01-19
Publication date: 2002-04-17
Anticipated expiration: 2011-01-19

Abstract

The utility model discloses a cell culture device which is assembled with sectional components, comprising a main body, a cover body, a transparent flat plate and a bar pad. A culture medium can form a running culture in a space formed between the main body and the cover body by an entrance of the culture medium and an exit of the culture medium. The utility model is provided with an automatic temperature monitoring, feedback and controlling system to control the temperature of the cell culture environment with the temperature control range from 5 to 85 degrees. Compared with the static culture, the cell vitro cultured by the utility model can adapt with the ambient organization and environment better after the material, cell enter into the body. The utility model can also be used for the dynamic culture of bacteria.

Description

A kind of cell culture apparatus

The utility model relates to a kind of cell culture apparatus.

Organizational project is a new branch of Materials science, be that the engineering science principle is learned the new subject that principle combines with cytology and tissue biological, be the artificial organ and the organ of the alternative defectiveness of research (as aging, damaged or morbid state), thereby final preparation can substitute their tissue and the science of organ.Since organizational project can improve people quality of life, have significant science, social and potential is commercial, therefore become the forward position of the boundary science that receive much attention.

The biomaterial of screening high comprehensive performance and preparation are the keys of organizational project by the complex body of biomaterial and cellularity.At present, people get used to preparing in static state the complex body of biomaterial and cell, and investigate the overall target of biomaterial under static conditions.Because cell and the required ambient stress of tissue regeneration have been ignored in static experiment, have departed from actual physiological environment, thereby the data under such testing data and the real physiological environment have certain deviation.Therefore, behind material-cell conjugate implanting to human body that vitro culture obtains, can be difficult to adapt to the tissue and the physiological environment of periphery.Experiment shows has the stress existence of denying can cause distinct result in material-cell conjugate forming process.

The purpose of this utility model is to provide a kind of device that can carry out cell cultures under dynamic environment.Use this device under the condition of certain controlled stress, to prepare material-cell conjugate.

A kind of cell culture apparatus of the present utility model, form by split type assembly close fit, described device main body has cavity and is positioned at first observation window in this cavity mid-way, the first observation window outer has first groove in cavity, on the two lateral walls of described main body, have medium flow inlet and medium flow outlet, described medium flow inlet and medium flow outlet communicate with cavity, on described first observation window, be stamped first transparent plate and second transparent plate, first transparent plate is placed in first groove, has first seal washer between first transparent plate and first observation window, the bar shaped pad that has two parallel placements between first transparent plate and second transparent plate, bar shaped pad placement direction is consistent with medium flow inlet and medium flow Way out, the lid of described device has shape, size and the corresponding projection of cavity wall, described lid has second observation window in the position corresponding with first observation window of main body, lid and main body are assembled into one with device for fastening, has second seal washer between the lid and second transparent plate, has the 3rd seal washer between lid and main body, assembling back first transparent plate upper surface and first groove outer surface maintenance level, the second transparent plate lower surface and described protrusion surface keep level, can adopt the adjustment slab-thickness, the realization that cooperatively interacts of depth of groove or rising height.

On lid and main body, also can have second groove, the 3rd groove, the 4th groove with described first seal washer, second seal washer, the 3rd seal washer contact position, this groove is used for fixing seal washer, adopt seal washer can make each assembly of assembling back device be able to strict seal and cooperate, the space that substratum can form between main body and lid forms current system by medium flow inlet and medium flow outlet; Described first transparent plate is preferably the polymethyl acrylate material, and the material of second transparent plate is preferably transparent materials such as simple glass, quartz or synthetic glass; The preferred of described first observation window, second observation window is rectangle, and preferred size is 70 * 35 square millimeters; Described bar shaped pad can be used for regulating the spacing of described first transparent plate and second transparent plate, and preferred material is a polytetrafluoroethylmaterial material, and preferred thickness is 0.2～0.5 millimeter; Described material of main part preferred metal materials is as copper, aluminium, steel etc.

It is 5-85 ℃ automatic temp monitoring, feedback and temperature controlling system that described device also can be furnished with the controllable temperature scope, can on described lid upper surface, be fixed with the electric heating heat block, have the jack of laying temperature transmitter on main body wall, the pair cell culture environment is carried out temperature control.

Preferred heights after the described device assembling is 10-15mm, and length is 200-100mm, and wide is 50-100mm.

Utilize the utility model device can under dynamic environment, carry out cell cultures, also can be complementary simultaneously with micro-automated imaging and image analysis system, by first observation window, second observation window institute's cultured cells situation is observed monitoring, because the kinetics of media flow can strict be controlled, cell adhesion and process of growth can be described with mathematical model.During media flow, the shearing force that is added on the cell can accurately obtain by calculating.Behind material-cell (as human skin fibrocyte and endotheliocyte-composite body) implanting to human body, can more adapt to the tissue and the physiological environment of periphery than static cultivation with this device vitro culture.This device also can be used for the dynamic cultivation of bacterium.

Below in conjunction with accompanying drawing the utility model is further described.

Fig. 1 is the vertical view of the utility model cell culture apparatus main body

Fig. 2 is the sectional view of cell culture apparatus main body shown in Figure 1 along the A-A line

Fig. 3 is the sectional view of cell culture apparatus main body shown in Figure 1 along the B-B line

Fig. 4 is the upward view of the utility model cell culture apparatus lid

Fig. 5 is the sectional view of cell culture apparatus lid shown in Figure 4 along the C-C line

Fig. 6 is the sectional view of cell culture apparatus lid shown in Figure 4 along the D-D line

Fig. 7 is the transverse sectional view after the assembling of the utility model cell culture apparatus

Fig. 8 is the longitudinal sectional view after the assembling of the utility model cell culture apparatus

A kind of cell culture apparatus of the present utility model, form by split type assembly close fit, this device main body 1 has cavity 2, first observation window 3, first groove 24 and threaded hole 22, on the two lateral walls of main body, have medium flow inlet 4 and medium flow outlet 5, described medium flow inlet 4 and medium flow outlet 5 communicate with cavity 2, on first observation window 3, be stamped first transparent plate 6 and second transparent plate 7, first transparent plate 6 and 3 of first observation windows have first seal washer 8, first

transparent plate

6 and 7 of second transparent plates have the bar shaped pad 9 of two parallel placements, 10, bar shaped pad 9, it is parallel that 10 placement directions and medium flow inlet 4 and medium flow export 5 directions, the lid 11 of this device has shape, size with the corresponding projection 12 of cavity 2 walls of main body 1, also have second observation window 13 and screw mounting hole 23 on the lid 11, be integral with screw 21 fastening assembling said modules, second observation window 13 and 7 of second transparent plates have second seal washer 14, and 1 of lid 11 and main body have the 3rd seal washer 15; On lid 11 and main body 1, have

groove

16,17,18 with described first seal washer 8, second seal washer 14, the 3rd seal washer 15 contact positions; First transparent plate 6 is the polymethyl acrylate material, and second transparent plate 7 is a simple glass; First observation window 3, second observation window 13 be rectangle, be of a size of 75 * 35 square millimeters; Bar shaped

pad

9,10 materials are polytetrafluoroethylmaterial material, and thickness is 0.3 millimeter; Packing ring is a silastic material, and main body 1 material is a copper.Being fixed with electric heating heat block 19 on lid 11 upper surfaces in addition, having temperature sensor jack 20 on main body 1 sidewall, is that 5-85 ℃ automatic temp monitoring, feedback and temperature controlling system carries out temperature control by the controllable temperature scope.

Claims

1. cell culture apparatus, form by split type assembly close fit, described device main body (1) has cavity (2) and is positioned at first observation window (3) in this cavity (2) mid-way, first observation window (3) outer has first groove (24) in cavity (2), on the two lateral walls of described main body (1), have medium flow inlet (4) and medium flow outlet (5), described medium flow inlet (4) and medium flow outlet (5) communicate with cavity (2), on described first observation window (3), be stamped first transparent plate (6) and second transparent plate (7), first transparent plate (6) is placed in first groove (24), has first seal washer (8) between first transparent plate (6) and first observation window (3), the bar shaped pad (9) (10) that has two parallel placements between first transparent plate (6) and second transparent plate (7), bar shaped pad (9) (10) placement direction is consistent with medium flow inlet (4) and medium flow outlet (5) direction, the lid of described device (11) has shape, size and the corresponding projection of cavity (2) wall (12), described lid (11) has second observation window (13) in the position corresponding with first observation window (3) of main body, lid (11) is assembled into one with main body (1) with device for fastening, (7) have second seal washer (14) between second observation window (13) and second transparent plate, has the 3rd seal washer (15) between lid and main body, assembling back first transparent plate (6) upper surface and the surface maintenance of first groove (24) outer level, second transparent plate (7) lower surface and the surperficial maintenance level of described projection (12).

2. according to a kind of cell culture apparatus of claim 1, it is characterized in that described lid (11) and main body (1) go up and described first seal washer (8), second seal washer (14), the 3rd seal washer (15) contact position have groove (16) (17) (18).

3. according to a kind of cell culture apparatus of claim 1, it is characterized in that described first transparent plate (6) is the polymethyl acrylate material, described second transparent plate (7) is simple glass, quartz or synthetic glass transparent material.

4. according to a kind of cell culture apparatus of claim 1, it is characterized in that described first observation window (3) and second observation window (13) are rectangle.

5. according to a kind of cell culture apparatus of claim 1, the material that it is characterized in that described bar shaped pad (9) (10) is a polytetrafluoroethylmaterial material, and thickness is 0.2～0.5 millimeter.

6. according to a kind of cell culture apparatus of claim 1, it is characterized in that the material of described main body (1) and lid (11) is a metallic substance.

7. according to any one described cell culture apparatus of claim 1～6, it is characterized in that on described lid (11) upper surface, being fixed with electric heating heat block (19), on main body wall, have temperature sensor jack (20).