CN2476818Y - Biochemical material analytical instrument based on micro-electrophoresis chip - Google Patents
Biochemical material analytical instrument based on micro-electrophoresis chip Download PDFInfo
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- CN2476818Y CN2476818Y CN 01224640 CN01224640U CN2476818Y CN 2476818 Y CN2476818 Y CN 2476818Y CN 01224640 CN01224640 CN 01224640 CN 01224640 U CN01224640 U CN 01224640U CN 2476818 Y CN2476818 Y CN 2476818Y
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 15
- 239000000463 material Substances 0.000 title description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 24
- 239000010703 silicon Substances 0.000 claims abstract description 24
- 239000000126 substance Substances 0.000 claims abstract description 12
- 238000005259 measurement Methods 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 4
- 230000003750 conditioning effect Effects 0.000 abstract description 2
- 238000013016 damping Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 238000005251 capillar electrophoresis Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004226 microchip electrophoresis Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
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- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
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- 230000003321 amplification Effects 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Abstract
The utility model relates to a biochemical substance analyser which bases on micro electrophoresis chip. The analyser comprises a computer measurement and control unit, a high-voltage module unit, a micro electrophoresis chip detecting unit and a signal conditioning unit. Wherein, a high voltage protection circuit in the high-voltage module consists of three high-voltage silicon stacks and a current-limiting resistance. The high voltage protection circuit in the utility model can raise high voltage output to more than 10000V without prolonging the switching time of a high voltage relay.
Description
The utility model relates to a kind of biochemical substances analyser based on little electrophoresis chip, belongs to field of analytic instrument.
The Capillary Electrophoresis that grows up the end of the eighties (capillary electrophoresis) is a kind of novel sample separation analytical technology.Its principle is under electric field action, utilizes the difference of each composition migration rate in damping fluid in the biochemical substances sample and realizes separating, and the ion to be checked that separates in the sample of back detects by the detecting device that is located at kapillary one end successively.It can be used for detection in Gene Mutation, hereditary disease and diagnosing tumor, and blood analysis, biological inoculum is identified, drug screening and pharmacological research, multiple fields such as protein and foranalysis of nucleic acids.Developed the electrophoresis chip technology beginning of the nineties, compare with the capillary electrophoresis technique of routine, can integrated sample on a chip and the mixing of detectable, reaction, a series of functions such as separation and analysis, parallel processing capability with a plurality of biochemical substances samples, because its good thermal diffusivity, can on pipeline, apply higher voltage, sample analysis speed has obtained large increase, make online detection become possibility, can be directly used in clinical diagnosis, legal medical expert identifies, a plurality of fields such as environment measuring and battlefield detecting, also be the developing direction of capillary electrophoresis technique from now on, dropped into a large amount of manpowers at present abroad, material resources are engaged in the research of microchip and detection system thereof, and it will have positive impetus to development of life science.
Used high-voltage power supply is single channel output on the general biochemical substances detecting instrument based on little electrophoresis chip, form four the road or more multichannel output by voltage divider and high-voltage relay, because high-voltage relay can produce the effluve phenomenon when switching between different high-voltage values, pulse current and due to voltage spikes appear in circuit, cause the high-voltage power supply power down, be that high-voltage power supply output descends, can damage high-voltage power supply when serious.In order to protect high-voltage power supply; generally take following measure: the high pressure export-restriction below 5000V, or is prolonged high-voltage relay switching time, reduce the amplitude of pulse current and high pressure spike; thereby improve the amplitude of high pressure output, but do the separation efficiency that can influence the biochemical substances sample like this.
The purpose of this utility model is a kind of biochemical substances analyser based on little electrophoresis chip of design, compares with biochemical substances analyser of the same type, makes it have the high pressure easy switching, the characteristics that signal noise is little.
The biochemical substances analyser based on little electrophoresis chip of the utility model design, comprise computer measurement and control unit, high-pressure modular unit, little electrophoresis chip optical detection unit and signal condition unit composition, wherein, the computer measurement and control unit comprises computing machine, D/A output and A/D input; The high-pressure modular unit links to each other with A/D with D/A respectively with the signal condition unit; High-pressure modular comprises two groups of high-voltage control circuits, and every group of high-voltage control circuit comprises high-voltage power supply, high tension protection circuit and voltage divider, switches by relay between two groups of high-voltage power supplies.It is characterized in that the high tension protection circuit in the high-pressure modular is made up of three high voltage silicon rectifier stacks and a current-limiting resistance.The positive pole of high voltage silicon rectifier stack a wherein links to each other with the positive pole of high-voltage power supply, the negative pole of high voltage silicon rectifier stack c links to each other with high voltage negative, and the both positive and negative polarity of high voltage silicon rectifier stack b links to each other with the both positive and negative polarity of high voltage silicon rectifier stack c, a respectively, the positive pole of high voltage silicon rectifier stack b connects output negative pole, and negative pole links to each other with output cathode by series connection one current-limiting resistance.
The high tension protection circuit that adopts in the biochemical substances analyser based on little electrophoresis chip of the utility model design when not prolonging high-voltage relay switching time, can make high pressure output bring up to more than the 10000V.
Description of drawings:
Fig. 1 is the Micro Chip Electrophoresis Analyser schematic diagram.
Fig. 2 is the circuit block diagram of high-pressure modular unit.
Fig. 3 is high-tension circuit figure, a among the figure, and b, c are high voltage silicon rectifier stack, d is a current-limiting resistance.
Below in conjunction with accompanying drawing, describe content of the present utility model in detail.
Among Fig. 1,1 is sample waste liquid end, and 2 is damping fluid liquid storage end, and 3 is sample liquid storage end, and 4 is damping fluid waste liquid end, and 5 is laser instrument.
As shown in Figure 1, this instrument comprises computer measurement and control unit, high-pressure modular unit, little electrophoresis chip optical detection unit and signal condition unit composition.Wherein, the computer measurement and control unit comprises computing machine, D/A output and A/D input; The high-pressure modular unit links to each other with A/D with D/A respectively with the signal condition unit.As shown in Figure 2, high-pressure modular comprises two groups of high-voltage control circuits, and every group of high-voltage control circuit comprises high-voltage power supply, high tension protection circuit and voltage divider, switches by relay between two groups of high-voltage power supplies.High tension protection circuit in the high-pressure modular is made up of three high voltage silicon rectifier stacks and a current-limiting resistance.As shown in Figure 3, the positive pole of high voltage silicon rectifier stack a links to each other with the positive pole of high-voltage power supply, the negative pole of high voltage silicon rectifier stack c links to each other with high voltage negative, and the both positive and negative polarity of high voltage silicon rectifier stack b links to each other with the both positive and negative polarity of high voltage silicon rectifier stack c, a respectively, the positive pole of high voltage silicon rectifier stack b connects output negative pole, and negative pole links to each other with output cathode by series connection one current-limiting resistance.
The Micro Chip Electrophoresis Analyser principle of work is: high-voltage power supply output is added on the microchip by one group of high-voltage relay, be used for the injection of sample and damping fluid, can power up four liquid storage holes simultaneously as required, and can add power mode when separating in the mode switch that powers up when annotating sample under the automatic control of computing machine.Electrophoresis chip is placed on the Three Degree Of Freedom fine motion objective table, LASER Light Source focuses on chip position to be detected with about 45 ° incident angle, excite the fluorescence of generation to enter photomultiplier (PMT) detecting device by optical filter by object lens collection back, by delivering to A/D converter after signal amplification and the conditioning, under computer control, finish signals collecting, storage and data processing, and can demonstrate the sample separation spectrogram in real time sample separation.After experiment is finished, annotate the sample cleaning unit automatically by chip again chip is cleaned, for use next time.
As shown in Figure 2, high-pressure modular is used for the injection of sample and damping fluid, and it cut-offs, switching state and amplitude are subjected to computer system monitoring and control.High-pressure modular is realized shielding, and transmits signal with computer system by the photoelectricity coupling, has so both guaranteed unify operator's personal safety of department of computer science, can eliminate the interference of high-pressure modular to signals collecting again.
High-voltage relay has when switching between the contact of different magnitudes of voltage, owing to have electric capacity between high-voltage relay and the ground, have charge and discharge phenomena in the circuit, produce current impulse and due to voltage spikes, when switching to high voltage, will produce negative pulse current in the load by low voltage.When switching to low voltage, can produce positive pulse current in the load by high voltage.When pulse current high-voltage power supply electric current permissible value, the high-voltage power supply output voltage will descend, and when the output of pulse current direction and high-voltage power supply is reverse, might damage high-voltage power supply.Therefore the output terminal in high-voltage power supply has designed the holding circuit of being made up of three high voltage silicon rectifier stacks.When inverse current occurring in the circuit, electric current will form discharge loop by high voltage silicon rectifier stack 2, and high voltage silicon rectifier stack 1 and 3 has stoped pulse current to flow to high-voltage power supply.When due to voltage spikes occurring in the circuit,, protected high-voltage power supply because high voltage silicon rectifier stack 1 and 3 is clamped down on.High-voltage output end is connected to 10K Ω～1M Ω current-limiting resistance, has reduced the amplitude of current impulse, makes pulse current value be lower than high-voltage power supply electric current permissible value.
Claims (1)
1, a kind of biochemical substances analyser based on little electrophoresis chip comprises computer measurement and control unit, high-pressure modular unit, little electrophoresis chip optical detection unit and signal condition unit composition; Wherein, the computer measurement and control unit comprises computing machine, D/A output and A/D input; The high-pressure modular unit links to each other with A/D with D/A respectively with the signal condition unit; High-pressure modular comprises two groups of high-voltage control circuits, and every group of high-voltage control circuit comprises high-voltage power supply, high tension protection circuit and voltage divider, switches by relay between two groups of high-voltage power supplies; It is characterized in that the high tension protection circuit in the high-pressure modular is made up of three high voltage silicon rectifier stacks and a current-limiting resistance; The positive pole of high voltage silicon rectifier stack a wherein links to each other with the positive pole of high-voltage power supply, the negative pole of high voltage silicon rectifier stack c links to each other with high voltage negative, and the both positive and negative polarity of high voltage silicon rectifier stack b links to each other with the both positive and negative polarity of high voltage silicon rectifier stack c, a respectively, the positive pole of high voltage silicon rectifier stack b connects output negative pole, and negative pole links to each other with output cathode by series connection one current-limiting resistance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01224640 CN2476818Y (en) | 2001-05-25 | 2001-05-25 | Biochemical material analytical instrument based on micro-electrophoresis chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01224640 CN2476818Y (en) | 2001-05-25 | 2001-05-25 | Biochemical material analytical instrument based on micro-electrophoresis chip |
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| Publication Number | Publication Date |
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| CN2476818Y true CN2476818Y (en) | 2002-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 01224640 Expired - Fee Related CN2476818Y (en) | 2001-05-25 | 2001-05-25 | Biochemical material analytical instrument based on micro-electrophoresis chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100445727C (en) * | 2003-12-30 | 2008-12-24 | 中国科学院上海微系统与信息技术研究所 | Microbiochemical detection and analysis method and instrument |
| CN101441194B (en) * | 2008-12-25 | 2012-12-26 | 重庆大学 | Low voltage chip electrophoresis multi-section circulation type voltage exerting control system and method |
-
2001
- 2001-05-25 CN CN 01224640 patent/CN2476818Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100445727C (en) * | 2003-12-30 | 2008-12-24 | 中国科学院上海微系统与信息技术研究所 | Microbiochemical detection and analysis method and instrument |
| CN101441194B (en) * | 2008-12-25 | 2012-12-26 | 重庆大学 | Low voltage chip electrophoresis multi-section circulation type voltage exerting control system and method |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |