CN2432613Y - Internal heating pressure jet liquid nitrogen program temp.-reducing device - Google Patents
Internal heating pressure jet liquid nitrogen program temp.-reducing device Download PDFInfo
- Publication number
- CN2432613Y CN2432613Y CN 00218101 CN00218101U CN2432613Y CN 2432613 Y CN2432613 Y CN 2432613Y CN 00218101 CN00218101 CN 00218101 CN 00218101 U CN00218101 U CN 00218101U CN 2432613 Y CN2432613 Y CN 2432613Y
- Authority
- CN
- China
- Prior art keywords
- liquid nitrogen
- control device
- utility
- model
- cyclone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model relates to an internal heating pressure jet liquid nitrogen program temperature reducing device, which is used for the fields, such as the biologic field, the medical care field, the pastoralism field, the aquatic production field, the food field, etc. The utility model is characterized in that the utility model is composed of a data collection and control device, a cyclone, a thermocouple, a heat insulated tank, a supporting frame, a liquid nitrogen conveying tube, a heating wire, and a liquid nitrogen container. The heating wire and the liquid nitrogen conveying tube are fixed in and arranged in the liquid nitrogen container. The heating wire is connected with the data collection and control device. The opening of the liquid nitrogen conveying tube enters from the top of the heat insulated tank. The liquid nitrogen conveying tube is provided with the cyclone. The supporting frame is arranged in the heat insulated tank. The thermocouple is connected with the data collection and control device. The utility model has the advantages of simple structure, low cost, and reliable performance, without a low temperature electromagnetic valve or a liquid nitrogen conveying pipeline for high voltage nitrogen.
Description
The utility model is a kind of interior heating and pressurizing injecting type liquid nitrogen programmed cooling instrument that is widely used in fields such as biology, medical treatment, herding, aquatic products, food.
Cryobiology studies show that, when carrying out the cryopreservation of biomaterial, different cells requires different cooling and rewarming program, and some complicated cells also require cooling process to be divided into multistage to carry out, and every section has different cooldown rates.In order to satisfy the requirement of biomaterial cryopreservation temperature-fall period, by development of computer, people have progressively succeeded in developing with micro-processor controlled programmed cooling instrument, and it has become a key equipment realizing biomaterial cryopreservation technology.Difference according to cooling and control principle, cooling instrument at present commonly used roughly can be divided into two classes: 1. liquid nitrogen spray formula cooling instrument, it is that people such as GuenterBaartz by the U.S. succeeded in developing the end of the seventies, and this instrument mainly is made up of liquid nitrogen induction system, control system and cold chamber.2. utilize the lift cooling instrument of gas phase zone thermograde in the liquid nitrogen container, it is to succeed in developing and applied for patent ZL91215370.9 by Shanghai University of Science and Technology, and liquid nitrogen conveying device and cold chamber unite two into one in this instrument.Above-mentioned two types programmed cooling instrument also exposes weakness separately when departments such as biology, medical treatment, herding, aquatic products, food are widely used.Liquid nitrogen spray formula cooling instrument costs an arm and a leg, owing in this instrument liquid nitrogen transfer pipeline low-temperature solenoid valve is installed, this solenoid valve is to be operated under the low temperature situation again, and water in air steam can dewfall, frosting on valve, can damage the coil and the Circuits System of solenoid valve; Situation such as also cause that easily valve closing is not forbidden; General biomaterial is reduced to-60 ℃ or-80 ℃ with the lift cooling instrument, can directly enter liquid nitrogen and preserve, but for liquid nitrogen spray formula cooling instrument, when reaching this temperature, be placed in the liquid nitrogen container the easy like this temperature recovery that causes after biomaterial need being taken out by the cooling instrument cooling chamber; Carry out a few hours even a couple of days continuous utmost point control procedure test of lowering the temperature at a slow speed for needs,,, in temperature-fall period, need to replenish liquid nitrogen, can disturb cooling process to the Dewar liquid nitrogen container because of liquid nitrogen consumption is bigger if adopt the injecting type cooling instrument.And adopt the lift cooling instrument, and its test specimen carries out in the gas phase zone in liquid nitrogen container, and the size of sample and shape just are subjected to the constraint of liquid nitrogen container spatial geometric shape like this; Gas phase zone is to communicate with air in the liquid nitrogen container, and its thermograde is vulnerable to the influence of surrounding environment, and the size of the thermograde direction of sample in liquid nitrogen container can not be too big.
Therefore, the utility model provides a kind of interior heating and pressurizing injecting type programmed cooling instrument in order to overcome the existing shortcoming of aforesaid two types cooling instrument, and it can either carry out the sample batches test, accomplishes that again experimentation cost is low and experimental performance is reliable simultaneously.
The technical solution of the utility model is achieved like this, and it is made of data acquisition and control device, cyclone, thermopair, insulated cabinet, support, defeated liquid nitrogen pipe, heater strip and liquid nitrogen container.The heater strip and the defeated liquid nitrogen pipe that are used for the liquid nitrogen heating fix, with the liquid nitrogen container that places filled with liquid nitrogen, the heater strip upper end is connected with control device with data acquisition, the transfusion nitrogen mouth of pipe enters insulated cabinet from the top, be equipped with cyclone at its place of entering, so that air-flow rotates in case, insulated cabinet is built-in with the support that is used for placing the object that is cooled and is used to measure the thermopair of the object temperature that is cooled, and thermopair is connected with control device with data acquisition.
The principle of this programme is: the heater strip that is fixed on defeated liquid nitrogen pipe can send certain heat, and after the liquid nitrogen in the liquid nitrogen container obtained heat, velocity of evaporation was accelerated, thereby the pressure in the liquid nitrogen container is increased.Like this, the liquid nitrogen pressurized is after defeated liquid nitrogen pipe enters insulated cabinet, and under the cyclone effect, the gentle nitrogen mixture of liquid nitrogen descends at the casing inside spin, makes temperature even.The amount of heat that electric heater applied is according to the difference of the temperature program(me) of the object temperature that is cooled of thermopair actual measurement in the insulated cabinet and setting, is regulated by control system.Change the heating power of heater strip, just can change the pressure in the liquid nitrogen container, thereby also just can control the amount of liquid nitrogen, reach the purpose of the adiabatic the temperature inside the box of control through defeated liquid nitrogen pipe.Above-mentioned principle designs owing to the utility model is based on, the ingenious natural rerum natura of liquid nitrogen of utilizing of liquid nitrogen induction system is carried liquid nitrogen, therefore, this programme is not to be provided with to cause that easily the low-temperature solenoid valve that liquid nitrogen stops up and leaks adds and gas nitrogen hydraulic fluid nitrogen transfer pipeline, make this programme simple in structure like this, cost is low, dependable performance.
The utility model embodiment is described further in conjunction with the accompanying drawings.
Fig. 1 is interior heating and pressurizing injecting type liquid nitrogen programmed cooling instrument structure principle chart,
Fig. 2 is the heater strip structural drawing;
Fig. 3 is heating power control principle figure;
Fig. 4 is the segmentation cooling illustration of cooling instrument.
By shown in Figure 1, interior heating and pressurizing injecting type programmed cooling instrument is made of data acquisition and control device 1, cyclone 2, thermopair 3, insulated cabinet 5, support 6, defeated liquid nitrogen pipe 8, heater strip 9 and liquid nitrogen container 10, the heater strip 9 that is used for the liquid nitrogen heating fixes with the liquid nitrogen container 10 that places filled with liquid nitrogen 7 with defeated liquid nitrogen pipe 8, and defeated liquid nitrogen pipe 8 mouths of pipe enter from insulated cabinet 5 tops.Be equipped with cyclone 2 at its place of entering, insulated cabinet 5 is built-in with the support 6 that is used for placing the object 4 that is cooled, and the thermopair 3 that is used to measure object 4 temperature that are cooled is connected with control device 1 with data acquisition.By shown in Figure 2, heater strip 9 two ends are connected with lead 11,12, and are provided with protection overcoat 13 and the interior cover 14 of protection.By shown in Figure 3, add thermal control units and form by comparison amplifier 15, triode 16, solid-state relay 17 and AC power 18, heater strip 9 is connected with solid-state relay 17 and AC power 18.Here, the temperature of thermopair real time record is the thermoelectrical potential signal, and it compares through comparison amplifier 15 with preset value after amplifier amplifies.If fiducial value is greater than zero, then triode 16 conductings, thus make heater strip 9 heating; If fiducial value is smaller or equal to zero, then triode 16 ends, and heater strip 9 stops heating.The setting of preset value and change are inserted computer with program in advance, and the program of cooling is inserted computer too in advance.Fig. 4 is the segmentation cooling illustration of cooling instrument, and section 1 represented among the figure is for to be cooled to 0 ℃ from initial temperature (25 ℃) with 1 ℃ of per minute; Section 2 is for to be cooled to-20 ℃ from 0 ℃ with 5 ℃ of per minutes; Section 3 is for to be cooled to-40 ℃ from-20 ℃ with 10 ℃ of per minutes; Section 4 is for to be cooled to-60 ℃ from-40 ℃ with 20 ℃ of per minutes; Section 5 is for to be cooled to-100 ℃ from-60 ℃ with 10 ℃ of per minutes.
Claims (2)
1, a kind of by data acquisition and control device, the liquid nitrogen conveying device, the interior heating and pressurizing injecting type liquid nitrogen programmed cooling instrument that cold chamber is formed, it is characterized in that, it is by data acquisition and control device (1), cyclone (2), thermopair (3), insulated cabinet (5), support (6), defeated liquid nitrogen pipe (8), heater strip (9) and liquid nitrogen container (10) constitute, the heater strip (9) that is used for the liquid nitrogen heating fixes with defeated liquid nitrogen pipe (8), with the liquid nitrogen container (10) that places filled with liquid nitrogen (7), defeated liquid nitrogen pipe (8) mouth of pipe enters from insulated cabinet (5) top, be equipped with cyclone (2) at the place of entering, insulated cabinet (5) is built-in with the support (6) that is used for placing the object (4) that is cooled, and the thermopair (3) that is used to measure object (4) temperature that is cooled is connected with control device (1) with data acquisition.
2, interior heating and pressurizing injecting type liquid nitrogen programmed cooling instrument according to claim 1, it is characterized in that, the thermal control units that adds in described data acquisition and the control device (1) is made up of comparison amplifier (15), triode (16), solid-state relay (17) and AC power (18), and heater strip (9) is connected with solid-state relay (17) and AC power (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00218101 CN2432613Y (en) | 2000-06-20 | 2000-06-20 | Internal heating pressure jet liquid nitrogen program temp.-reducing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00218101 CN2432613Y (en) | 2000-06-20 | 2000-06-20 | Internal heating pressure jet liquid nitrogen program temp.-reducing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2432613Y true CN2432613Y (en) | 2001-05-30 |
Family
ID=33583555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00218101 Expired - Fee Related CN2432613Y (en) | 2000-06-20 | 2000-06-20 | Internal heating pressure jet liquid nitrogen program temp.-reducing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2432613Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015081478A1 (en) * | 2013-12-03 | 2015-06-11 | Linde Aktiengesellschaft | Device for treatment of parts at low temperatures |
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
-
2000
- 2000-06-20 CN CN 00218101 patent/CN2432613Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015081478A1 (en) * | 2013-12-03 | 2015-06-11 | Linde Aktiengesellschaft | Device for treatment of parts at low temperatures |
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104677000B (en) | Liquid nitrogen cooling cryogenic device and implementation method for same | |
| CN103366917B (en) | Refrigerating plant and high-temperature superconducting magnet refrigerating plant | |
| CN106769395A (en) | For material at low temperature static mechanical property, continuously strain records test system with image | |
| CN2432613Y (en) | Internal heating pressure jet liquid nitrogen program temp.-reducing device | |
| CN202865235U (en) | Waterproof constant-temperature incubator | |
| CN207096150U (en) | The underwater heat-insulating property experimental rig of underground heat composite thermal pipeline | |
| CN201136864Y (en) | Programmed cell freezing instrument with quick temperature reduction and temperature recovery functions | |
| CN206496958U (en) | For material at low temperature static mechanical property, continuously strain records test system with image | |
| JP2017531493A (en) | Apparatus and method for helium collection and reliquefaction in a magnetoencephalograph | |
| CN109188322A (en) | A kind of thermal cycling test device of the superconducting magnet component of controlled rate | |
| CN109238340A (en) | The integrated environment test system of Mars probes product | |
| CN211354554U (en) | Cabinet is stored to intelligence sample | |
| CN203204467U (en) | Programmed temperature lowering instrument | |
| CN208488459U (en) | The moisture and salt regime laboratory testing rig of changeable temperature | |
| CN108709352A (en) | A kind of modified liquid nitrogen refrigerating system | |
| CN204702759U (en) | Microbiological incubator | |
| CN110196309B (en) | Diagenetic simulation experiment device capable of controlling fluid flow under high-temperature and high-pressure conditions | |
| CN207987234U (en) | A kind of medical Micro biological Tests quarantine culture apparatus | |
| CN207850963U (en) | Textile thermal property test device under a kind of low temperature environment | |
| CN207036869U (en) | A kind of full-automatic liquefied ammonia sampling analysis device | |
| CN207280990U (en) | Temperature Field Control Xia Han areas Groundwater movement simulator | |
| CN109827369A (en) | A kind of Medical Transport cold chain energy-saving bag | |
| CN203337442U (en) | Small-sized freeze dryer for freezing and drying few samples in laboratory | |
| Gill et al. | Design and performance of a portable frost chamber for the investigation of hardiness of strawberries | |
| CN2051345U (en) | Simple temperature-falling freezer for biotic preservation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |