CN2351450Y - Bacteria gathering culturer - Google Patents
Bacteria gathering culturer Download PDFInfo
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- CN2351450Y CN2351450Y CN 98250902 CN98250902U CN2351450Y CN 2351450 Y CN2351450 Y CN 2351450Y CN 98250902 CN98250902 CN 98250902 CN 98250902 U CN98250902 U CN 98250902U CN 2351450 Y CN2351450 Y CN 2351450Y
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- incubator
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Abstract
The utility model discloses a bacteria gathering incubator which is suitable for using when sterility tests are carried out in a medicine system. The utility model mainly comprises a transparent casing, a base, a sieve plate, a microporous filtering film and an air filter. The utility model is characterized in that the casing of the incubator and the base are movably connected, the air filter and a permeation opening on the casing are movably connected, and the connection of the permeation opening and the air filter can be one kind of movable connection, such as screwed connection, intervening connection, etc. The utility model not only solves a problem that a traditional filter is easy to pollute in an operation process, but also solves a problem that the checking cost of a disposable bacteria gathering incubator is high, and the utility model can be used by a plurality of producing enterprises, use units and quality checking mechanisms for pharmaceutical products, biological products, blood products, etc. in China.
Description
The utility model relates to a kind of device of medical sterility test, particularly a kind of colony culture device.
At present, membrane-filter procedure is one of medicine method of comprising sterility tests such as biological products, blood products and recombinant product, the membrane-filter procedure that the Chinese Pharmacopoeia appendix records all adopts traditional opening membrane filter, after sample solution filters, needing that filter membrane is cut into some cultivates respectively, pollute easily in the operating process, thereby influence assay.Developed countries such as U.S., day, English mainly use the dead front type membrane filter, this strainer collection filters the collection bacterium and is incubated at one, this kind dead front type membrane filter is disposable using appts, existing commodity listing, it mainly is the product of U.S. MILLIPORE company, it is characterized in that with two or three monomers be that a cover is used with peristaltic pump, each monomer divides parts such as incubator shell, base, rack and millipore filtration, and bonding being integral, cover top portion has an injection port and that the ventilation mouth of filter membrane is housed, and base has a liquid outlet etc.This device one cover price is approximately about 200 yuan, so seem expensive because of belonging to disposable using appts, general medicine, blood products and biological products quality test unit all can not accept, and mainly are that some joint venture are used.The Ye Dalin of Zhejiang Hangzhou has developed the disposable filtering colony culture device, and applied for patent, the patent No. is respectively zl93210917.9, zl93219087.1 etc., and the product that he develops and the product way of realization of MILLIPORE company are similar, and price reduces greatly.However, its two or two is a cover, and price is approximately 20-30 unit, and the cost of sterility test is increased, and it is popularized be restricted.
The purpose of this utility model provides the reusable colony culture device of a kind of energy, this incubator can make the test liquid body filter and carry out microbial culture therein, in use can satisfy the filtration needs of malleation and negative pressure simultaneously, and can guarantee with extraneous ventilation property not contaminated.
The utility model is achieved in that on the basis of existing technology the shell with colony culture device becomes movable the connection with base, and what the connection of the two can be in screw thread, clamping or the connection of reeve formula is wherein a kind of.Beveled is postponed in penetrating mouthful lower end in order to flexibly connect for air filter and cover top portion penetrating mouthful, and what shell and penetrating mouthful be connected can be in clamping or the connections of reeve formula is a kind of.
Technical progress of the present utility model is: because this colony culture device shell connects with base system is movable, so millipore filtration is replaceable, incubator can be reused, effect is identical with existing disposable dead front type incubator, and consumptive material each time (as filter membrane etc.) cost is suitable with traditional opening strainer.After each the use,, use the appropriate means sterilization, can re-use through cleaning, changing film.Each sterilization method that more renews behind the filter membrane is identical with disposable incubator, promptly sterilizes with proper method such as oxyethane, can guarantee the specification of quality that incubator is aseptic equally.So both solved traditional opening strainer easy pollution problems in operating process, solved the high problem of inspection cost again, manufacturing enterprise, applying unit and the quality test mechanism of domestic most medicine, biological products and blood products etc. can be born.
The utility model will be further described now to reach embodiment in conjunction with the accompanying drawings.
Fig. 1 is the cut-away view of single membrane filtration colony culture device.
Fig. 2 is the structural representation of second embodiment of the utility model.
In Fig. 1, the shell of membrane filtration colony culture device (7) and base (8) connect for movable, can or inject a kind of in the connection for screw thread, clamping.Cover top portion is penetrating mouthful to be connected for movable with the air filter that millipore filtration is housed, and it act as the millipore filtration that is equipped with in the air filter obstruct bacterium and guarantee ventilation property inside and outside the incubator simultaneously.Injection port outer end and elasticity are than pipe coupling, shell is formed with transparent plastics or glass, and sidewall is carved with the scale (2) of indication capacity, and the sieve plate (4) that supports millipore filtration is arranged on the base, the effect of millipore filtration is to hold back the bacterium that may exist in the test solution, and liquid outlet is positioned at the bottom of base.First embodiment of the utility model is the preparation of doing before using: open connecting of incubator shell and base, make its separation, after cleaning, the drying, new millipore filtration is put on the sieve plate, put shell again, shell is connected compress millipore filtration to make the connection place sealing with base, with using after the proper method sterilization virus killing.
As follows in conjunction with its embodiment of Fig. 2: as the injection port and of three incubators to be advanced three go out the formula four-way pipe and be connected, three incubators are arranged side by side, the dead air filter port, the sample introduction port of four-way pipe is connected with supplying test agent, three of four-way is gone out flexible tube section snap in the peristaltic pump pump head, under the effect of peristaltic pump, test solution enters in three membrane filtration colony culture devices by the four-way flexible pipe, and test solution is discharged through liquid outlet by millipore filtration under the effect of malleation.After filtration is finished, inaccessible liquid outlet.The sample introduction port of four-way pipe is shifted out from supply the container of test agent, be connected, open air filter opening,, substratum is injected in the film colony culture device, go into till the scale of specified amount by the effect of peristaltic pump with substratum.Remove the four-way flexible pipe, inaccessible injection port places incubator under the defined terms and to cultivate, and whether check has bacterial growth.Collect filtrate as need, the liquid outlet and three of three incubators can be advanced one and go out the formula four-way pipe and be connected, at exit end collection liquid.
Claims (5)
1, a kind of colony culture device comprises transparent outer cover, base, sieve plate, millipore filtration and air filter, it is characterized in that incubator shell (7) and base (8) are flexible connection.
2, incubator according to claim 1 is characterized in that described air filter (5) connects for movable with the penetrating mouth of cover top portion.
3, incubator according to claim 1 is characterized in that the shell of described incubator is connected to wherein a kind of that screw thread, clamping or reeve formula are connected with base.
4, incubator according to claim 1 is characterized in that two of described incubator cover top portion penetrating mouthful (1) and lower end identical with (6) shape postpone into beveled.
5, incubator according to claim 2, it is characterized in that described incubator top penetrating mouthful and air filter be connected to wherein a kind of that screw thread, reeve formula are connected.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 98250902 CN2351450Y (en) | 1998-12-23 | 1998-12-23 | Bacteria gathering culturer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 98250902 CN2351450Y (en) | 1998-12-23 | 1998-12-23 | Bacteria gathering culturer |
Publications (1)
Publication Number | Publication Date |
---|---|
CN2351450Y true CN2351450Y (en) | 1999-12-01 |
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ID=33995197
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 98250902 Expired - Fee Related CN2351450Y (en) | 1998-12-23 | 1998-12-23 | Bacteria gathering culturer |
Country Status (1)
Country | Link |
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CN (1) | CN2351450Y (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816846A (en) * | 2010-04-30 | 2010-09-01 | 贵州科伦药业有限公司 | Sterile filtering device |
CN108744988A (en) * | 2018-08-15 | 2018-11-06 | 河南省医疗器械检验所 | Air filtering device for transfusion/film liquid bacterial interception capacity experimental test component |
CN114717103A (en) * | 2022-04-11 | 2022-07-08 | 浙江泰林生命科学有限公司 | Automatic change aseptic inspection device |
-
1998
- 1998-12-23 CN CN 98250902 patent/CN2351450Y/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101816846A (en) * | 2010-04-30 | 2010-09-01 | 贵州科伦药业有限公司 | Sterile filtering device |
CN108744988A (en) * | 2018-08-15 | 2018-11-06 | 河南省医疗器械检验所 | Air filtering device for transfusion/film liquid bacterial interception capacity experimental test component |
CN108744988B (en) * | 2018-08-15 | 2023-10-24 | 河南省药品医疗器械检验院(河南省疫苗批签中心) | Air filter/membrane liquid bacteria interception capability test module for transfusion |
CN114717103A (en) * | 2022-04-11 | 2022-07-08 | 浙江泰林生命科学有限公司 | Automatic change aseptic inspection device |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C26 | Partial revocation of patent right or utility model | ||
RP01 | Partial revocation of patent right |
Decision date of revocation: 20030110 |
|
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |