CN2319407Y - Liquid/liquid diffusion process crystallizing room for growth of space protein crystal - Google Patents
Liquid/liquid diffusion process crystallizing room for growth of space protein crystal Download PDFInfo
- Publication number
- CN2319407Y CN2319407Y CN 98201791 CN98201791U CN2319407Y CN 2319407 Y CN2319407 Y CN 2319407Y CN 98201791 CN98201791 CN 98201791 CN 98201791 U CN98201791 U CN 98201791U CN 2319407 Y CN2319407 Y CN 2319407Y
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- Prior art keywords
- liquid
- moving plate
- diffusion process
- price fixing
- pond
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- 239000007788 liquid Substances 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 42
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 42
- 239000013078 crystal Substances 0.000 title claims abstract description 26
- 238000009792 diffusion process Methods 0.000 title claims abstract description 22
- 238000002425 crystallisation Methods 0.000 claims description 27
- 238000003825 pressing Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 4
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000009827 uniform distribution Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 238000001556 precipitation Methods 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- 239000012460 protein solution Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 description 7
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002050 diffraction method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005486 microgravity Effects 0.000 description 2
- 238000002424 x-ray crystallography Methods 0.000 description 2
- 102000006947 Histones Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Crystals, And After-Treatments Of Crystals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The utility model relates to a liquid/liquid diffusion process crystallizing chamber for growth of space protein crystals, which comprises a fixed disk and a movable disk. The upper surface of the movable disk is provided with predefined number of cylindrical protein solution tanks according to the predefined positions, the fixed disk is provided with predefined number of precipitation reagent solution tanks which are communicated from top to bottom according to the predefined positions, and rubber stoppers are inserted on the precipitation reagent solution tanks. A circular pressure plate is arranged on the rubber stoppers, the circular pressure plate is fixed on the upper surface of the fixed disk, the number of the protein solution tanks and the number of the precipitation reagent solution tanks are equal, the fixed disk is buckled above the movable disk, and a crystallizing chamber unit is formed. The utility model has the advantages of compact structure, low cost, and less space occupation.
Description
The utility model belongs to the protein crystal growth crystallisation chamber of space biological technical field, is meant a kind of protein crystal growth in space liquid/liquid diffusion process crystallisation chamber especially.
Protein is the basic substance of life, is the basal component of forming all organisms.Protein molecule is made up of thousands of atoms, and it is arranged and forms very complicated spatial structure.The space structure of protein molecule has determined its biological function.
The most important ordinary method of measuring the biomacromolecule space structure is the X-ray crystallography method, utilize the single crystal diffraction method to study biological macromolecular space structure, the protein of being studied to be purified earlier, and cultivate the single crystal that becomes to be suitable for diffraction analysis, and then the collection X ray diffracting data, carry out structure determination.Because the development of high speed X ray surface detector and super-minicomputer significantly reduces the used time of Collection and analysis data.Grow suitable single crystal, become the key issue in the whole X-ray crystallography, usually influence the process of whole work owing to this link, in addition to certain protein because of can not get available crystal, and can't utilize X-ray diffraction method to come analytical structure.
Protein soln reaches limited hypersaturated state lentamente, produces small number of crystal nucleus, and grows up into crystal, and this is the process of protein crystal formation.
The distinctive microgravity condition in space avoided the interference of flow phenomenon for crystallisation process, also avoided because depositional phenomenon and container wall to the detrimentally affect of crystal growth, thereby more help crystal growth.Much test and studies show that, utilize the microgravity condition growing single-crystal, can improve the homogeneity of crystal habit, reduce the crystalline defective, increase the size of monocrystalline.
The utility model crystallisation chamber can be carried out liquid/liquid diffusion process crystal growth, and its principle is as follows: Fig. 1 is the schematic diagram that utilizes liquid/liquid diffusion method for growing protein crystal.Its principle is that protein soln is placed protein soln pond 4, and precipitant solution places precipitant solution pond 5, and the diffusion by two liquid interface solute molecules makes the protein soln supersaturation separate out nucleus, and grows up into monocrystalline.
Normally used space crystallization apparatus structure is not compact, and wherein crystallisation chamber partly takes up room greatlyyer, and function also is difficult to complete, gives user's choice few.Because the chance of space experiment is still less and costly, if having multi-functional, volume is little and lightweight protein liquid/liquid diffusion process crystallisation chamber, then can save a large amount of time and moneys.
The purpose of this utility model is, a kind of protein crystal growth in space liquid/diffusion process liquid crystallisation chamber is provided, and it has the advantage of compact construction.
A purpose more of the present utility model is, a kind of protein crystal growth in space liquid/liquid diffusion process crystallisation chamber is provided, and it can be assembled into the multiwalled structure, thereby has reduced volume, has also just saved a large amount of time and moneys.
The utility model provides a kind of protein crystal growth in space with liquid/liquid diffusion process crystallisation chamber, and comprising having: a Moving plate is provided with the cylindrical protein soln pond of predetermined number at the upper surface of Moving plate; One price fixing, on price fixing, be provided with the precipitant solution pond of predetermined number with the corresponding position of Moving plate, the precipitant solution pond is a shape of through holes, above the precipitant solution pond, be inserted with the rubber plug that is shorter in length than the precipitant solution pond, on rubber plug, be fixed with a diameter less than Moving plate diameter and the middle circular pressing plate that a circular hole is arranged with screw and Moving plate upper surface; Price fixing is fastened on Moving plate, forms liquid/liquid diffusion process crystallisation chamber unit; Wherein this protein soln pond and precipitant solution pond are by circumference and radius uniform distribution, and the quantity of the solution pool of each circle equates that the solution pool of an adjacent circle is not in same radius, for being interspersed; The centre of the radially section of price fixing is a boss shape, the height that the height of boss is tightened up a screw greater than circular pressing plate; There is the axis hole of a band keyway center of Moving plate and price fixing, and a pilot hole is arranged on the sidewall of price fixing; Price fixing and Moving plate are transparent material and make.
For further understanding feature of the present utility model and effect, also the utility model is described in further detail in conjunction with the accompanying drawings to lift following examples now, wherein:
Fig. 1 is the prior art constructions schematic diagram;
Fig. 2 is a structural representation of the present utility model;
Fig. 3 is the vertical view of Fig. 2;
Fig. 4 is the side cutaway view after Fig. 2 multilayer assembling;
See also Fig. 2 to Fig. 4, be structural representation of the present utility model.Comprising having: a Moving plate 21 and a price fixing 22, this Moving plate 21 is with price fixing 22 that transparent material is made and diameter is identical, this Moving plate 21 and price fixing 22 are the right cylinder of flat shape, upper surface at Moving plate 21 is provided with the equally distributed cylindrical protein soln of multilayer pond 24 along circumference and radius, is provided with 27 protein soln ponds 24 in the present embodiment altogether; On price fixing 22, be provided with the precipitant solution pond 23 that equates with protein soln pond 24 numbers with the 24 corresponding positions, protein soln pond of Moving plate 21, precipitant solution pond 23 is a shape of through holes, be inserted with the rubber plug 25 that is shorter in length than the precipitant solution pond above precipitant solution pond 23, remaining space, rubber plug 25 backs is inserted in precipitant solution pond 23 can put precipitant solution; Upper surface with screw 27 and price fixing 22 on rubber plug 25 is fixed with a diameter less than price fixing 22 diameters and the middle circular pressing plate 26 that a circular hole is arranged, and its effect is to prevent that rubber plug 25 from coming off from precipitant solution pond 23; Described price fixing 22 is fastened on the Moving plate 21, makes it be combined into liquid/liquid diffusion process crystallisation chamber unit; Wherein protein soln pond 24 is to enclose and the radius uniform distribution by circle with precipitant solution pond 23, the protein soln pond 24 of each circle and the quantity in precipitant solution pond 23 equate, the protein soln pond 24 of an adjacent circle and precipitant solution pond 23 be not in same radius, for being interspersed, the centre of the radially section of this price fixing 22 is a boss shape, and the height of boss is greater than tighten up a screw 27 height of circular pressing plate 26.The center of this Moving plate 22 and price fixing 21 has the axis hole 28 of a band keyway, in axis hole 28, penetrate one, get final product the position of relative fixed Moving plate 21 and price fixing 22, this Moving plate 21 and price fixing 22 can pivot along axle, can adjust the connection and the blocking-up in protein soln pond 24 and precipitant solution pond 23 by this; On the sidewall of price fixing 22, be provided with a pilot hole 31; When many histones of needs matter crystallisation chamber unit, can be assembled into the multiwalled shape according to the structure of Fig. 4, present embodiment is for using 3 groups of structures that liquid/liquid diffusion process crystallisation chamber unit stacks together.Wherein with after liquid/liquid diffusion process crystallisation chamber unit stacks together, heart place places an axis 44 therein, and axis 44 is relative affixed with each Moving plate 21 usefulness one flat key 47, in the unitary centers of many group crystallisation chamber, has placed an axis 44; Cover plate 48,42 about in the of one is arranged, in the both sides of cover plate 48,42 up and down 2 columns 45 are arranged, screw 49 used respectively by column 45 and cover plate 48,42 is affixed up and down, described cover plate 48,42 up and down is circular, and diameter is greater than liquid/unitary diameter of liquid diffusion process crystallisation chamber, one handle 46 with price fixing 22 and column 45 relative positionings, is equipped with in the upper end of axis 44 by a screw 43 in pilot hole 31 places on column 45 and price fixing 22.
Working process of the present utility model is for loading onto protein soln in the protein soln pond 24 of Moving plate 21, in the precipitant solution pond 23 on price fixing 22 precipitant solution is housed, when protein soln pond 24 is rotated to the position that communicates with precipitant solution pond 23, protein soln begins crystallization, when crystallization finished, turning handle 46 drove angle of Moving plate 21 rotations and makes protein soln pond 24 and 23 dislocation of precipitant solution pond, block its connection, crystallisation process finishes.
The big I in the precipitant solution pond 23 in the utility model is controlled its capacity by the length of adjusting rubber plug 25, reaches what purpose of control precipitant solution.
The utility model compared with prior art, have compact conformation, cost is low and occupy little space excellent The point.
Claims (5)
1, a kind of protein crystal growth in space is characterized in that with liquid/liquid diffusion process crystallisation chamber, includes: a Moving plate is provided with the cylindrical protein soln pond of predetermined number at the upper surface of Moving plate; One price fixing, on price fixing, be provided with the precipitant solution pond of predetermined number with the corresponding position of Moving plate, the precipitant solution pond is a shape of through holes, above the precipitant solution pond, be inserted with the rubber plug that is shorter in length than the precipitant solution pond, on rubber plug, be fixed with a diameter less than Moving plate diameter and the middle circular pressing plate that a circular hole is arranged with screw and Moving plate upper surface; Price fixing is fastened on Moving plate, forms liquid/liquid diffusion process crystallisation chamber unit.
2, by the described protein crystal growth in space of claim 1 liquid/liquid diffusion process crystallisation chamber, it is characterized in that, wherein this protein soln pond and precipitant solution pond are by circumference and radius uniform distribution, the quantity of the solution pool of each circle equates, the solution pool of an adjacent circle is not in same radius, for being interspersed.
3, by the described protein crystal growth in space of claim 1 liquid/liquid diffusion process crystallisation chamber, it is characterized in that the centre of the radially section of price fixing is a boss shape, the height that the height of boss is tightened up a screw greater than circular pressing plate.
By the described protein crystal growth in space of claim 1 liquid/liquid diffusion process crystallization treasured, it is characterized in that 4, there is the axis hole of a band keyway center of Moving plate and price fixing, and a pilot hole is arranged on the sidewall of price fixing.
5, by the described protein crystal growth in space of claim 1 liquid/liquid diffusion process crystallisation chamber, it is characterized in that price fixing and Moving plate are transparent material and make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98201791 CN2319407Y (en) | 1998-03-06 | 1998-03-06 | Liquid/liquid diffusion process crystallizing room for growth of space protein crystal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98201791 CN2319407Y (en) | 1998-03-06 | 1998-03-06 | Liquid/liquid diffusion process crystallizing room for growth of space protein crystal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2319407Y true CN2319407Y (en) | 1999-05-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98201791 Expired - Fee Related CN2319407Y (en) | 1998-03-06 | 1998-03-06 | Liquid/liquid diffusion process crystallizing room for growth of space protein crystal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2319407Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254274A (en) * | 2012-02-17 | 2013-08-21 | 仓怀兴 | Regulation and optimization apparatus for protein crystallization |
| CN104152980A (en) * | 2014-07-21 | 2014-11-19 | 清华大学 | Liquid-phase diffusion single-crystal growth sample loading method and special sample loading tube therefor |
-
1998
- 1998-03-06 CN CN 98201791 patent/CN2319407Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254274A (en) * | 2012-02-17 | 2013-08-21 | 仓怀兴 | Regulation and optimization apparatus for protein crystallization |
| CN103254274B (en) * | 2012-02-17 | 2015-04-01 | 中国科学院生物物理研究所 | Regulation and optimization apparatus for protein crystallization |
| CN104152980A (en) * | 2014-07-21 | 2014-11-19 | 清华大学 | Liquid-phase diffusion single-crystal growth sample loading method and special sample loading tube therefor |
| CN104152980B (en) * | 2014-07-21 | 2016-06-15 | 清华大学 | Liquid phase diffusion crystal growth application of sample method and special application of sample pipe |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |