CN2368847Y - Crystallizer for aerospace growth of protein crystal - Google Patents
Crystallizer for aerospace growth of protein crystal Download PDFInfo
- Publication number
- CN2368847Y CN2368847Y CN 99211710 CN99211710U CN2368847Y CN 2368847 Y CN2368847 Y CN 2368847Y CN 99211710 CN99211710 CN 99211710 CN 99211710 U CN99211710 U CN 99211710U CN 2368847 Y CN2368847 Y CN 2368847Y
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- Prior art keywords
- pond
- central shaft
- protein
- crystallisation chamber
- space
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- 102000004169 proteins and genes Human genes 0.000 title claims description 54
- 108090000623 proteins and genes Proteins 0.000 title claims description 54
- 239000013078 crystal Substances 0.000 title claims description 27
- 238000002425 crystallisation Methods 0.000 claims abstract description 36
- 230000008025 crystallization Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 3
- 238000002050 diffraction method Methods 0.000 description 2
- 230000005486 microgravity Effects 0.000 description 2
- 238000002424 x-ray crystallography Methods 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Images
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- Peptides Or Proteins (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
Abstract
The utility model comprises a right end cover, a left end cover, a cylinder body and a central shaft. The utility model is characterized in that the right end cover is covered at the right end of the cylinder body, and the left end cover is fixedly connected with the left end of the cylinder body; the center positions of the left and the right end covers are penetrated with one central shaft; the left end of the central shaft is connected with a driving gear, and the right end of the central shaft is pivoted with a bearing; a plurality of groups of crystallization chamber units are sheathed and arranged on the central shaft and between the left and the right end covers. The utility model has the advantages of compact structure, low cost and little occupation space.
Description
The utility model provides the protein crystal growth crystallisation chamber, refers in particular to a kind of protein crystal growth in space crystallisation chamber.
Protein is the basic substance of life, is the basal component of forming all organisms.Protein molecule is made up of thousands of atoms, and it is arranged and forms very complicated spatial structure.The space structure of protein molecule has determined its biological function.
The most important ordinary method of measuring the biomacromolecule space structure is the X-ray crystallography method, utilize the single crystal diffraction method to study biological macromolecular space structure, the protein of being studied to be purified earlier, and cultivate the single crystal that becomes to be suitable for diffraction analysis, and then the collection X ray diffracting data, carry out structure determination.Because the development of high speed X ray surface detector and super-minicomputer significantly reduces the used time of Collection and analysis data.Grow suitable single crystal, become the key issue in the whole X-ray crystallography, usually influence the process of whole work owing to this link, in addition to certain protein because of can not get available crystal, and can't utilize X-ray diffraction method to come analytical structure.
Protein soln reaches limited hypersaturated state lentamente, produces small number of crystal nucleus, and grows up into crystal, and this is the process of protein crystal formation.
The distinctive microgravity condition in space avoided the interference of flow phenomenon for crystallisation process, also avoided because depositional phenomenon and container wall to the detrimentally affect of crystal growth, thereby more help crystal growth.Much test and studies show that, utilize the microgravity condition growing single-crystal, can improve the homogeneity of crystal habit, reduce the crystalline defective, increase the size of monocrystalline.
Present normally used space crystallization apparatus structure is not compact, and wherein crystallisation chamber partly takes up room greatlyyer, and function also is difficult to complete, gives user's choice few.Because the chance of space experiment is less and costly, if having multi-functional, volume is little and lightweight crystallization of protein chamber, then can save a large amount of time and moneys.
Main purpose of the present utility model provides a kind of protein crystal growth in space crystallisation chamber, and it has the advantage of compact construction.
Another purpose of the present utility model provides a kind of protein crystal growth in space crystallisation chamber, and it can be assembled into the multiwalled structure, thereby has reduced volume, has also just saved a large amount of time and moneys.
For reaching above-mentioned purpose, a kind of protein crystal growth in space crystallisation chamber of the utility model, comprising having: right end cap, left end cap, cylindrical shell, central shaft, it is characterized in that, wherein the right-hand member housing is buckled in the right-hand member of cylindrical shell, and left end cap and cylindrical shell left end are affixed, have placed a central shaft in the center of left and right sides end cap, the left end of this central shaft is connected with transmitting gear, the right-hand member of this central shaft and bearing hinge joint; Between the end cap of the left and right sides, be equipped with many group crystallisation chamber unit on the central shaft.
Wherein this crystallisation chamber unit includes price fixing and Moving plate, has the protein soln pond on price fixing, has the precipitant solution pond on Moving plate.
Wherein the group number in protein soln pond and precipitant solution pond is 1: 5.
Wherein the protein soln pond on the price fixing is shaped as semisphere and square.
Wherein on price fixing square protein soln pond radially have a lamp pond, in this lamp pond a lamp is housed.
The semisphere protein soln pond of wherein on price fixing, opening quantity be one or three.
Wherein the position in corresponding square protein soln pond has vision slit on the sidewall of this cylindrical shell.
Wherein the position that central shaft is corresponding with Moving plate is equipped with driving key, is equipped with a spring on central shaft between right end cap and the price fixing.
For further specifying structure of the present utility model and feature, below in conjunction with accompanying drawing the utility model is done one and describe in detail, wherein:
Fig. 1 is a combination section of the present utility model.
Fig. 2 is the unitary synoptic diagram of crystallisation chamber among the utility model Fig. 1, shows that dome-type protein soln pond is three a state, and the precipitant solution pond is five a state.
Fig. 3 is the unitary synoptic diagram of crystallisation chamber among the utility model Fig. 1, shows the state in square protein soln pond and lamp pond, and the precipitant solution pond is five a state.
Fig. 4 is the unitary synoptic diagram of crystallisation chamber among the utility model Fig. 1, shows that dome-type protein soln pond is one a state, and the precipitant solution pond is five a state.
At first see also shown in Figure 1, a kind of protein crystal growth in space crystallisation chamber of the utility model, comprising having: right end cap 1, left end cap 3, cylindrical shell 2, central shaft 11, it is characterized in that wherein right end cap 1 cover is buckled in the right-hand member of cylindrical shell 2, and fastening with screw, left end cap 3 is affixed with cylindrical shell 2 left ends, placed a central shaft 11 in the center of left and right sides end cap 1,3, the left end of this central shaft 11 is connected with transmitting gear 4, and the right-hand member of this central shaft 11 and bearing 5 articulate; Between the left and right sides end cap 1,3, be equipped with many group crystallisation chamber unit 30,40,50 on the central shaft 11.
Please shown in Figure 2 again in conjunction with consulting, wherein this crystallisation chamber unit 30 includes price fixing 31 and Moving plate 32, have one group of semisphere protein soln pond 311 on price fixing 31, the quantity in semisphere protein soln pond 311 is three, and is evenly distributed in the scope in a precipitant solution pond 321; On Moving plate 32, have precipitant solution pond 321, the quantity in this precipitant solution pond 321 is five, this precipitant solution pond 321 be shaped as the fan-shaped semisphere protein soln pond 311 that fastens a group of covering, this precipitant solution pond 321 distributes for being 60 degree, and does not have precipitant solution pond 321 in the scope of one of them 60 degree; The precipitant solution of different concns is housed in addition, so that be suitable for different crystallization speed in five precipitant solution ponds 321.The shape in the precipitant solution pond 321 in the crystallisation chamber unit 30,40,50 is identical, and quantity is five.
Other sees also shown in Figure 3, wherein the structure of Moving plate 32 is identical, being shaped as of protein soln pond 411 on the price fixing 41 is square, square protein soln pond 411 radially has a lamp pond 413 on price fixing 41, one lamp 414 is housed in this lamp pond 413, one cover cap 412 is arranged on the lamp pond, and this lamp 414 is the usefulness for the video camera illumination of installing in addition.
Wherein said price fixing and Moving plate are transparent material and make, as organic glass etc.
Fig. 4 and Fig. 2 are basic identical, and its difference is: the quantity in the semisphere protein soln pond 311 of wherein opening on price fixing 31 is one.
Wherein the position in corresponding square protein soln pond 411 has vision slit 22 (as shown in Figure 1) on the sidewall of this cylindrical shell 2, and these vision slit 22 nationalitys can write down crystallisation process by digital camera.Wherein central shaft 11 and each Moving plate 32 corresponding positions are equipped with driving key 12, this driving key 12 is to play to drive Moving plate 32 rotations when central shaft 11 rotates, be equipped with a spring 111 on central shaft 11 between right end cap 1 and the price fixing 31, it can play the effect that makes the relative sealing with price fixing of Moving plate.For reducing weight, can on the sidewall of cylindrical shell 2, have strip hole 21 in addition.
Working process of the present utility model is: the protein soln of packing in the dome-type protein soln pond 311 of price fixing 31,41,51 and square protein soln pond 411 is respectively charged into the precipitant solution of different concns in the precipitant solution pond 321 on Moving plate 32; When forwarding the protein soln pond to communicate position with the precipitant solution pond by control transmitting gear 4, protein soln begins crystallization, and its crystallization speed can be adjusted the precipitant solution pond that different concns is housed as required and forward the position that communicates with protein soln to.When crystallization finishes, by control transmitting gear 4 angle is rotated in protein soln pond and precipitant solution pond again, make the dislocation of protein soln pond and precipitant solution pond, block its connection, crystallisation process finishes.
The utility model compared with prior art has: compact conformation, cost is low and occupy little space Advantage.
Claims (8)
1. protein crystal growth in space crystallisation chamber, comprising having: right end cap, left end cap, cylindrical shell, central shaft, it is characterized in that, wherein the right-hand member housing is buckled in the right-hand member of cylindrical shell, left end cap and cylindrical shell left end are affixed, placed a central shaft in the center of left and right sides end cap, the left end of this central shaft is connected with transmitting gear, the right-hand member of this central shaft and bearing hinge joint; Between the end cap of the left and right sides, be equipped with many group crystallisation chamber unit on the central shaft.
2. protein crystal growth in space crystallisation chamber according to claim 1 is characterized in that, wherein this crystallisation chamber unit includes price fixing and Moving plate, has the protein soln pond on price fixing, has the precipitant solution pond on Moving plate.
3. protein crystal growth in space crystallisation chamber according to claim 2 is characterized in that, wherein the group number in protein soln pond and precipitant solution pond is 1: 5.
4. according to claim 1,2 or 3 described protein crystal growth in space crystallisation chamber, it is characterized in that wherein the protein soln pond on the price fixing is shaped as dome-type and square.
5. according to claim 1 or 4 described protein crystal growth in space crystallisation chamber, it is characterized in that, wherein on price fixing square protein soln pond radially have a lamp pond, in this lamp pond a lamp is housed.
6. according to claim 1,2 or 3 described protein crystal growth in space crystallisation chamber, it is characterized in that, the dome-type protein soln pond of wherein on price fixing, opening quantity be one or three.
7. protein crystal growth in space crystallisation chamber according to claim 1 is characterized in that, wherein the position in corresponding square protein soln pond has vision slit on the sidewall of this cylindrical shell.
8. protein crystal growth in space crystallisation chamber according to claim 1 is characterized in that wherein the position that central shaft is corresponding with Moving plate is equipped with driving key, is equipped with a spring on central shaft between right end cap and the price fixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99211710 CN2368847Y (en) | 1999-05-18 | 1999-05-18 | Crystallizer for aerospace growth of protein crystal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99211710 CN2368847Y (en) | 1999-05-18 | 1999-05-18 | Crystallizer for aerospace growth of protein crystal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2368847Y true CN2368847Y (en) | 2000-03-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99211710 Expired - Fee Related CN2368847Y (en) | 1999-05-18 | 1999-05-18 | Crystallizer for aerospace growth of protein crystal |
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|---|---|
| CN (1) | CN2368847Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254274A (en) * | 2012-02-17 | 2013-08-21 | 仓怀兴 | Regulation and optimization apparatus for protein crystallization |
| CN110256527A (en) * | 2019-07-23 | 2019-09-20 | 中国科学院生物物理研究所 | A kind of protein for situ X-Ray Diffraction is compound to educate brilliant box |
| CN110954249A (en) * | 2019-12-18 | 2020-04-03 | 东南大学 | Method for measuring in-vitro myocardial tissue contractility based on protein wire spring |
-
1999
- 1999-05-18 CN CN 99211710 patent/CN2368847Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254274A (en) * | 2012-02-17 | 2013-08-21 | 仓怀兴 | Regulation and optimization apparatus for protein crystallization |
| CN103254274B (en) * | 2012-02-17 | 2015-04-01 | 中国科学院生物物理研究所 | Regulation and optimization apparatus for protein crystallization |
| CN110256527A (en) * | 2019-07-23 | 2019-09-20 | 中国科学院生物物理研究所 | A kind of protein for situ X-Ray Diffraction is compound to educate brilliant box |
| CN110954249A (en) * | 2019-12-18 | 2020-04-03 | 东南大学 | Method for measuring in-vitro myocardial tissue contractility based on protein wire spring |
| CN110954249B (en) * | 2019-12-18 | 2021-04-06 | 东南大学 | Method for measuring in-vitro myocardial tissue contractility based on protein wire spring |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |