CN221275748U

CN221275748U - Cell sorting and separating device

Info

Publication number: CN221275748U
Application number: CN202322936234.4U
Authority: CN
Inventors: 董宇超; 刘艳芳; 郭猛; 孙沁莹; 陈铃
Original assignee: Second Military Medical University SMMU
Current assignee: Second Military Medical University SMMU
Filing date: 2023-10-31
Publication date: 2024-07-05
Anticipated expiration: 2033-10-31

Abstract

The utility model discloses a cell sorting and separating device, which comprises a box body, wherein a U-shaped frame is fixed at the top of the box body, a through hole is formed in the center of the top of the box body, a driving mechanism is arranged on a first vertical pipe, a lifting mechanism is arranged on a second vertical pipe, the cell sorting and separating device can drive an inner hollow pipe and a nozzle to rotate simultaneously by starting a rotating motor, the inner hollow pipe and the nozzle can move up and down circularly by starting a servo motor in a circulating way, an external pump is started at the same time, buffer solution in the buffer solution box is pumped out, and finally the buffer solution enters the inner hollow pipe through the second vertical pipe and the first vertical pipe and is sprayed out through the nozzles at the end part, the top and the bottom of the inner hollow pipe.

Description

Cell sorting and separating device

Technical Field

The utility model relates to the technical field of cell treatment, in particular to a cell sorting and separating device.

Background

The magnetic bead sorting is based on that cell surface antigen can be combined with specific monoclonal antibody connected with magnetic beads, in an external magnetic field, cells connected with the magnetic beads through antibodies are adsorbed and retained in the magnetic field, and cells without the surface antigen cannot be combined with the specific monoclonal antibody connected with the magnetic beads, but are not magnetic and not retained in the magnetic field, so that the cells can be separated.

If the Chinese patent with the bulletin number of CN210304148U is granted, a cell immunomagnetic bead sorting device is disclosed, the cell immunomagnetic bead sorting device comprises a base, two supporting plates are symmetrically arranged on the upper end face of the base, a fixing shell is commonly connected between the two supporting plates, four rectangular grooves are symmetrically formed in the inner wall of the fixing shell, electromagnets are fixedly arranged in each rectangular groove, and each electromagnet comprises an electromagnet S pole and an electromagnet N pole. According to the utility model, in the rotating process of the sorting tank, the cells without magnetic beads and the cells with magnetic beads in the sample liquid of the cells to be detected are more active, so that the sorting tank can sort the sample liquid of the cells to be detected more fully and thoroughly, and the situation that the sorted sample liquid still contains a lot of magnetic bead cells is avoided.

The above issued patents have the following problems in the use process:

Buffer solution can not be fully washed at the corners of the inner wall of the sorting tank, so that the previous liquid is remained, and the next cell sorting is affected due to the crossover.

Aiming at the problems, innovative design is urgently needed on the basis of the original cell sorting and separating device.

Disclosure of utility model

The present utility model is directed to a cell sorting and separating device, which solves the above-mentioned problems of the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cell sorting separator, includes the box, the top of box is fixed with U type frame, the center department at box top has seted up the through-hole, the inner wall of through-hole is fixed with the seal cover, the inside of seal cover is run through there is first standpipe, the lower extreme of first standpipe is located the internal connection of box has interior hollow tube, tip, top and the bottom of interior hollow tube all are provided with the nozzle, the second standpipe is installed through sealed bearing rotation in the top of first standpipe, the top in U type frame is run through to the upper end of second standpipe, be provided with actuating mechanism on the first standpipe, be provided with elevating system on the second standpipe.

Preferably, the driving mechanism comprises a first gear, the top of the outer wall of the first vertical tube is fixed with the first gear, one side of the first gear is meshed with a second gear, the bottom of the outer wall of the second vertical tube is fixed with a rotating motor, and the output end of the bottom of the rotating motor is fixedly connected with the top of the second gear.

Preferably, the elevating system includes the pivot, rotate between the top of box and the top in the U type frame and install the pivot, the upper end of pivot runs through the top of U type frame and is fixed with servo motor, servo motor's outer wall is fixed in the top of U type frame through the installation pole, the both sides of second standpipe all are fixed with the connecting plate, the inner wall of connecting plate and the outside interconnect of pivot, another the inside of connecting plate is run through there is the guide bar, the upper and lower end of guide bar respectively with the top in the U type frame and the top fixed connection of box.

Preferably, a feeding bin is arranged on one side of the top of the box body, and a sealing cover is arranged on the top of the feeding bin.

Preferably, a discharging pipe is arranged at the bottom of one side of the box body, and a manual valve is arranged on the discharging pipe.

Preferably, a protective shell is fixed on the outer wall of the box body, and an electromagnet is mounted in the protective shell.

Preferably, the rotating shaft is in threaded connection with the connecting plate, and the other connecting plate is in sliding connection with the guide rod.

Compared with the prior art, the utility model has the beneficial effects that: this separation device is selected separately to cell, through starting rotating electrical machines, rotating electrical machines can drive interior hollow tube and nozzle and rotate simultaneously, through starting servo motor circulation forward and backward, can make interior hollow tube and nozzle do the oscillometric movement, start external pump simultaneously, with the inside buffer solution of buffer solution case, the inside of interior hollow tube is entered into through second standpipe and first standpipe to the buffer solution, last through interior hollow tube tip, top and bottom nozzle blowout, because the nozzle can circulate the oscillometric movement in rotatory, thereby can accomplish the abundant washing of inner wall to the box, the inner wall of box has been avoided remaining last cell sap, influence the sorting of next cell.

Drawings

FIG. 1 is a schematic diagram of a front cross-sectional structure of the present utility model;

FIG. 2 is a schematic diagram of an electromagnet top view mounting structure according to the present utility model;

FIG. 3 is a schematic top view of the mounting structure of the connecting plate of the present utility model;

fig. 4 is a schematic top view of a first gear according to the present utility model.

In the figure: 1. a case; 2. a protective shell; 3. an electromagnet; 4. a U-shaped frame; 5. a through hole; 6. sealing sleeve; 7. a first standpipe; 8. an inner hollow tube; 9. a nozzle; 10. sealing the bearing; 11. a second standpipe; 12. a first gear; 13. a second gear; 14. a rotating electric machine; 15. a rotating shaft; 16. a connecting plate; 17. a guide rod; 18. a servo motor; 19. a feeding bin; 20. sealing cover; 21. a discharge pipe; 22. and (5) a manual valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-4, the present utility model provides a technical solution: the utility model provides a cell sorting separator, includes box 1, and the top of box 1 is fixed with U type frame 4, through-hole 5 has been seted up to the center department at box 1 top, and the inner wall of through-hole 5 is fixed with seal cover 6, and seal cover 6's inside is run through there is first standpipe 7, and the lower extreme of first standpipe 7 is located the internal connection of box 1 and has interior hollow tube 8, and interior hollow tube 8's tip, top and bottom all are provided with nozzle 9, and second standpipe 11 is installed through sealed bearing 10 rotation in the top of first standpipe 7, and the upper end of second standpipe 11 runs through in the top of U type frame 4, is provided with actuating mechanism on the first standpipe 7, is provided with elevating system on the second standpipe 11.

The driving mechanism comprises a first gear 12, a first gear 12 is fixed at the top of the outer wall of the first vertical pipe 7, a second gear 13 is meshed with one side of the first gear 12, a rotating motor 14 is fixed at the bottom of the outer wall of the second vertical pipe 11, and an output end at the bottom of the rotating motor 14 is fixedly connected with the top of the second gear 13.

The elevating system includes pivot 15, rotate between the top of box 1 and the top in the U type frame 4 and install pivot 15, the upper end of pivot 15 runs through the top of U type frame 4 and is fixed with servo motor 18, servo motor 18's outer wall is fixed in the top of U type frame 4 through the installation pole, the both sides of second standpipe 11 all are fixed with connecting plate 16, the inner wall of connecting plate 16 and the outside interconnect of pivot 15, the inside of another connecting plate 16 runs through there is guide bar 17, the upper and lower end of guide bar 17 respectively with the top in the U type frame 4 and the top fixed connection of box 1.

One side at the top of the box body 1 is provided with a feeding bin 19, the top of the feeding bin 19 is provided with a sealing cover 20, and cell sap can be fed into the box body 1 through the feeding bin 19.

The bottom of one side of the box body 1 is provided with a discharge pipe 21, a manual valve 22 is arranged on the discharge pipe 21, and separated cell liquid in the box body 1 can be taken out through the discharge pipe 21.

The outer wall of box 1 is fixed with protective housing 2, and the internally mounted of protective housing 2 has electro-magnet 3, and after the electro-magnet 3 circular telegram, can make the inner wall of box 1 produce magnetism and inhale, can adsorb the inside magnetic bead cell that contains of box 1.

The rotating shaft 15 is in threaded connection with the connecting plate 16, the other connecting plate 16 is in sliding connection with the guide rod 17, and when the rotating shaft 15 rotates positively or reversely, the connecting plate 16 can drive the second vertical pipe 11 to move upwards or downwards, so that the first vertical pipe 7, the inner hollow pipe 8 and the nozzle 9 on the second vertical pipe 11 can move upwards or downwards, and the buffer solution sprayed by the nozzle 9 can sufficiently clean the inner wall of the box body 1.

Working principle: when the cell sorting and separating device is used, firstly, cell liquid is put into the box body 1 through the feeding bin 19, then the electromagnet 3 is started, so that magnetic attraction is generated on the inner wall of the box body 1, cells containing magnetic beads in the cell liquid in the box body 1 are adsorbed on the inner wall of the box body 1, the manual valve 22 is rotated, so that the discharge pipe 21 is opened, at the moment, cells containing no magnetic beads in the cell liquid in the box body 1 are discharged through the discharge pipe 21, then the electromagnet 3 is closed, so that magnetic attraction cannot be generated on the inner wall of the box body 1, at the moment, the cells containing magnetic beads adsorbed on the inner wall of the box body 1 flow downwards and are discharged through the discharge pipe 21, and sorting and separation of the cells are completed;

When the inner wall of the box body 1 needs to be cleaned, the rotating motor 14 is started at this time, then the rotating motor 14 drives the second gear 13, the first gear 12, the first vertical pipe 7, the hollow pipe 8 and the nozzle 9 to rotate simultaneously, then the servo motor 18 is started to rotate positively and reversely, at this time, the servo motor 18 drives the rotating shaft 15 to rotate, because the rotating shaft 15 and the connecting plate 16 are in threaded connection, and the other connecting plate 16 and the guide rod 17 are in sliding connection, the connecting plate 16 can drive the second vertical pipe 11 to do up-and-down circular movement, so that the first vertical pipe 7, the hollow pipe 8 and the nozzle 9 on the second vertical pipe 11 can be made to do up-and-down circular movement, an external pump is started simultaneously, buffer liquid in the buffer liquid box is pumped out, and finally the buffer liquid enters the interior of the hollow pipe 8 through the second vertical pipe 11 and the first vertical pipe 7, and is sprayed out through the nozzle 9 at the end part, the top and the bottom of the hollow pipe 8, and the nozzle 9 can be circularly moved up-down while rotating, so that the inner wall of the box body 1 can be fully cleaned, cell liquid remaining last time on the inner wall of the box body 1 is avoided, and cell sorting of the next time is affected.

Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.

Claims

1. Cell sorting separator, including box (1), its characterized in that: the utility model discloses a novel energy-saving box, including box (1), sealing bearing, sealing sleeve (6), first standpipe (7), inner tube (8) are located at the top of box (1), U type frame (4) are fixed with at the top of box (1), through-hole (5) have been seted up to the center department at box (1) top, the inner wall of through-hole (5) is fixed with seal cover (6), the inside of seal cover (6) is run through and is had first standpipe (7), the lower extreme of first standpipe (7) is located the internal connection of box (1) and has hollow tube (8), tip, top and the bottom of hollow tube (8) all are provided with nozzle (9), second standpipe (11) are installed through sealed bearing (10) rotation in the top of first standpipe (7), the upper end of second standpipe (11) runs through in the top of U type frame (4), be provided with actuating mechanism on first standpipe (7), be provided with elevating system on second standpipe (11).

2. The cell sorting and separating device according to claim 1, wherein: the driving mechanism comprises a first gear (12), the top of the outer wall of the first vertical tube (7) is fixedly provided with the first gear (12), one side of the first gear (12) is meshed with a second gear (13), the bottom of the outer wall of the second vertical tube (11) is fixedly provided with a rotating motor (14), and the output end of the bottom of the rotating motor (14) is fixedly connected with the top of the second gear (13).

3. The cell sorting and separating device according to claim 1, wherein: the lifting mechanism comprises a rotating shaft (15), the rotating shaft (15) is rotatably arranged between the top of the box body (1) and the top in the U-shaped frame (4), a servo motor (18) is fixed at the top of the U-shaped frame (4) in a penetrating mode at the upper end of the rotating shaft (15), connecting plates (16) are fixed at the two sides of the second vertical tube (11) through mounting rods, the inner walls of the connecting plates (16) are connected with the outer sides of the rotating shaft (15), guide rods (17) penetrate through the inner portions of the connecting plates (16), and the upper end and the lower end of each guide rod (17) are fixedly connected with the top in the U-shaped frame (4) and the top of the box body (1) respectively.

4. The cell sorting and separating device according to claim 1, wherein: one side at the top of the box body (1) is provided with a feeding bin (19), and the top of the feeding bin (19) is provided with a sealing cover (20).

5. The cell sorting and separating device according to claim 1, wherein: a discharge pipe (21) is arranged at the bottom of one side of the box body (1), and a manual valve (22) is arranged on the discharge pipe (21).

6. The cell sorting and separating device according to claim 1, wherein: the outer wall of the box body (1) is fixed with a protective shell (2), and an electromagnet (3) is arranged in the protective shell (2).

7. A cell sorting and separating device according to claim 3, wherein: the rotating shaft (15) is in threaded connection with the connecting plate (16), and the other connecting plate (16) is in sliding connection with the guide rod (17).