CN221071508U

CN221071508U - Cell counter light source assembly

Info

Publication number: CN221071508U
Application number: CN202322684297.5U
Authority: CN
Inventors: 姚必庆; 姚欣妤; 余康睿
Original assignee: Hangzhou Miu Instruments Co ltd
Current assignee: Hangzhou Miu Instruments Co ltd
Priority date: 2023-10-08
Filing date: 2023-10-08
Publication date: 2024-06-04
Anticipated expiration: 2033-10-08

Abstract

The utility model discloses a cell counter light source assembly, which comprises an excitation light source, a bright field light source, a light source turntable and a motor for driving the light source turntable to rotate, wherein the excitation light source and the bright field light source are fixedly arranged on the light source turntable in a circumferential mode around the rotation axis of the light source turntable, and the number of the excitation light sources is at least two; at least one point on the upper side or the lower side of the light source turntable is used as a detection point, and the motor drives the light source turntable to rotate so as to control any excitation light source or bright field light source beam to be projected to the detection point. According to the utility model, by arranging the light source motor and the light source turntable to be matched with the excitation light source and the bright field light source, different excitation light sources and bright field light sources can be controlled to rotate to the light transmission detection position, the excitation light source is matched with the counting plate on the tray, and the excitation light source or the bright field light source is provided for fluorescent dye, so that the degree of automation is higher.

Description

Cell counter light source assembly

Technical Field

The utility model relates to the technical field of cell counting, in particular to the technical field of a light source assembly of a cell counter.

Background

The fluorescent cell counter is a common device in a medical laboratory and is mostly used for cell counting work during cell culture, and the key of accurate counting of the fluorescent cell counter is clear visual field and accurate algorithm. Among these, it is whether the focus is stable or not, and whether the magnification of the objective lens is sufficient or not, which affects the definition of the field of view.

A system for counting cells or biomolecules is disclosed in chinese patent application CN200980121707.5 (publication CN102089418 a), which discloses a system for counting cells or biomolecules, comprising: a covered chamber having a known height and configured to hold a biomolecule or cell suspension in a sample; at least one fluorescent light source connected to the at least one fluorescent light beam narrowing device; a bright field light source connected to the bright field beam narrowing device; a microscope objective; a detection device; a fluorescence filter assembly for allowing only excitation light to illuminate the sample and allowing only emission light from the sample to be imaged by the detection device; and a movable light shutter for blocking bright-field light during fluorescent detection. The working principle is as follows: after a sample is dyed by the dye, the living cells and the dead cells are marked with different dyes due to different properties, so that the living cells and the dead cells have different fluorescence characteristics, namely different excitation wavelengths and different emission wavelengths, and therefore, when the sample is excited by different light sources, a counting system can respectively collect bright-field images (images of total cell counts of cell communities) of the sample, fluorescent images of the living cells and fluorescent images of the dead cells, thereby facilitating cell counts.

However, for the cell counter, at least three light sources need to be configured in the light source module, if the light sources are manually switched, the efficiency is low, the imaging quality is easily affected due to human factor intervention, and the degree of automation is low.

Disclosure of Invention

The utility model aims to solve the problems in the prior art and provides a light source assembly of a cell counter, which can improve the automation degree of switching of the light source of the cell counter.

In order to achieve the above purpose, the utility model provides a cell counter light source assembly, which comprises an excitation light source, a bright field light source, a light source turntable and a motor for driving the light source turntable to rotate, wherein the excitation light source and the bright field light source are fixedly arranged on the light source turntable in a circumferential manner around the rotation axis of the light source turntable, and at least two excitation light sources are arranged;

At least one point on the upper side or the lower side of the light source turntable is used as a detection point, and the motor drives the light source turntable to rotate so as to control any excitation light source or bright field light source beam to be projected to the detection point.

Preferably, the light source turntable is further provided with a plurality of light source lamp holders, and the excitation light source is obliquely arranged on the light source lamp holders.

Preferably, each light source lamp holder is provided with two excitation light sources respectively, and the light beams projected by the excitation light sources on the same light source lamp holder can form an intersection at the detection point.

Preferably, the light source lamp bracket is provided with a Y-shaped bracket, and the two excitation light sources are respectively and oppositely arranged at two sides of the Y-shaped bracket.

Preferably, the excitation light source comprises an LED lamp panel, a lens for adjusting the light of the LED lamp panel into parallel light, and a light source filter for filtering light in a non-specified wavelength range.

The cell counter light source assembly has the beneficial effects that: according to the utility model, by arranging the light source motor and the light source turntable to be matched with the excitation light source and the bright field light source, different excitation light sources and bright field light sources can be controlled to rotate to the light transmission detection position, the excitation light source is matched with the counting plate on the tray, the excitation light source is provided for fluorescent dye to emit fluorescence, the bright field light source can also be controlled to rotate to the light transmission detection position, illumination is provided for the counting plate in a bright field detection mode, the light source is more convenient to switch, and the degree of automation is higher.

The features and advantages of the present utility model will be described in detail by way of example with reference to the accompanying drawings.

Drawings

FIG. 1 is a schematic diagram of the front perspective view of the whole machine of a light source assembly of a cytometer.

Fig. 2 is a schematic diagram of a rear perspective view of a whole machine of a light source assembly of a cytometer.

Fig. 3 is a schematic diagram of the front view of the whole machine of the light source assembly of the cell counter according to the present utility model.

FIG. 4 is a schematic perspective view of a light source module of a cytometer light source module according to the present utility model.

FIG. 5 is a schematic top view of a light source assembly of a cytometer light source assembly of the present utility model.

FIG. 6 is a schematic view of the cross-sectional structure A-A of FIG. 5.

FIG. 7 is a schematic perspective view of a light source module of a cytometer light source module of the present utility model.

FIG. 8 is a schematic view showing a front cross-sectional structure of a light source module of a cytometer light source module according to the present utility model.

FIG. 9 is a schematic perspective view of a tray of a light source assembly of a cytometer of the present utility model.

FIG. 10 is a schematic cross-sectional view of a tray of a light source assembly of a cytometer of the present utility model.

FIG. 11 is a schematic perspective view of a camera imaging assembly of a light source assembly of a cytometer in accordance with the present utility model.

FIG. 12 is a schematic perspective view of the internal slicing mechanism of the camera imaging assembly of the light source assembly of the cytometer of the present utility model.

FIG. 13 is a schematic perspective view of a counting plate of a light source assembly of a cytometer of the present utility model.

Wherein:

1-a main body; 10-a substrate; 11-a tray; 12-counting plate; 111-a fixed slot; 112-clamping blocks; 113-a micro switch; 121-a counting cell; 130-counting assembly screw rod structure; 131-a screw rod control motor; 132—a lead screw nut; 1121-a clamping groove; 20-an industrial camera; 21-a camera mount; 22-an objective lens; a 23-camera filter; 24-slice housing; 25-slicing turntable; 26-a camera support plate; 27-a camera screw mechanism; 28-a camera lifting motor; 30-an excitation light source; 31-a light source turntable; 32-a light source motor; 33-a light source lamp holder; 301-LED lamp panels; 302-a lens; 303-a light source filter; 40-bright field light source.

Description of the embodiments

The present utility model will be further described in detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the detailed description and specific examples, while indicating the utility model, are intended for purposes of illustration only and are not intended to limit the scope of the utility model. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present utility model.

In the description of the present utility model, it will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

In the description of the present utility model, it should be noted that the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships in which the inventive product is conventionally placed in use, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. The meaning of "a number" is one or more than one unless specifically defined otherwise.

In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Examples

Referring to fig. 1-12, a light source assembly of a cell counter according to the present utility model includes a main body 1, the main body 1 includes a light source assembly, a counting tray assembly and a camera imaging assembly, the counting tray assembly includes a base plate 10 and a counting plate assembly disposed on the base plate 10, the counting plate assembly includes a tray 11 and a counting plate 12 detachably disposed on the tray 11, a fixing slot 111 adapted to the counting plate 12 is disposed on the tray 11, the counting plate 12 is detachably disposed in the fixing slot 111, a plurality of counting cells 121 are disposed on the counting plate 12, a transparent detection position 101 adapted to the counting cells 121 is disposed on the base plate 10, the counting plate assembly further includes a moving assembly connected to the tray 11 and used for driving the tray 11 to translate along the upper side of the base plate 10 so as to control any one of the counting cells 121 to move to the transparent detection position 101, wherein the upper side of the transparent detection position 101 is used as a detection point in the present embodiment;

The light source assembly comprises eight excitation light sources 30, a bright field light source 40, a light source turntable 31 and a light source motor 32 for driving the light source turntable 31 to rotate, wherein the excitation light sources 30 and the bright field light source 40 are fixedly arranged on the light source turntable 31 in a circumferential mode around the rotation axis of the light source turntable 31, the excitation light sources 30 are driven by the light source motor 32 to rotate the light source turntable 31 so as to control any one of the excitation light sources 30 or the bright field light source 40 to project light beams to the light transmission detection position 101, and the camera imaging assembly is arranged towards the light transmission detection position 101. In this implementation, through setting up light source motor 32 and light source carousel 31 and excitation light source 30, bright field light source 40 cooperation, can control different excitation light sources 30, bright field light source 40 rotate to the printing opacity and examine position 101 department, thereby cooperate with the counter plate 12 on the tray 11, provide excitation light source for fluorescent dye, make the dyestuff send fluorescence, also can control bright field light source 40 rotation to the printing opacity and examine position 101 department, provide illumination for counter plate 12 under the mode that bright field detects, the light source switches over more conveniently, degree of automation is higher, through setting up movable assembly drive tray 11 and counter plate 12 translation, can control the translation of different counter tanks 121 of counter plate 12 to printing opacity and examine position 101 department, thereby detect with the cooperation of light source subassembly, realize automatically regulated counter plate 12, and is more convenient to use, camera imaging module is used for collecting the multiple image signal that produces on the cell counter plate, the fluorescent cell counter automation degree of this embodiment is high, and convenient to use can satisfy various different user demands.

Examples

Referring to fig. 1, 2 and 4, the light source turntable 31 is further provided with four light source lamp holders 33, and the excitation light source 30 is obliquely disposed on the light source lamp holders 33 relative to the camera imaging assembly. In this embodiment, the excitation light source 30 is obliquely arranged, so that oblique parallel excitation light does not irradiate the objective lens 22, and interference of the excitation light source to camera imaging is avoided.

Referring to fig. 4, 5 and 6, each light source holder 33 is provided with two excitation light sources 30, and the light beams projected by the excitation light sources 30 on the same light source holder 33 can form an intersection at the light transmission detection position 101. When one light source lamp holder 33 moves to the lower side of the light transmission detection position 101, two excitation light sources 30 on the light source lamp holder 33 can simultaneously face the light transmission detection position 101 and irradiate the counting plate 12 through the light transmission detection position 101, so that excitation irradiation is conveniently performed by controlling the opening and closing of the excitation light sources 30 on the light source lamp holder 33 to control different excitation light sources, and various detection requirements are met.

Referring to fig. 4, 5 and 6, the light source holder 33 is provided with a Y-shaped bracket 331, and the two excitation light sources 30 are respectively disposed at two opposite sides of the Y-shaped bracket 331. The Y-shaped bracket 331 is convenient for supporting the excitation light source 30, and the excitation light sources 30 on two sides of the upper end of the Y-shaped bracket 331 are arranged oppositely, so that the oppositely arranged excitation light sources 30 can realize that the light beams projected by the excitation light sources 30 on the same light source lamp bracket 33 can form an intersection at the light transmission detection position 101.

Referring to fig. 4, 5 and 6, the excitation light source 30 includes an LED lamp panel 301, a lens 302 for adjusting the light of the LED lamp panel 301 to parallel light, and a light source filter 303 for filtering light of a non-designated wavelength range. In operation, light emitted from the LED lamp panel 301 is changed to parallel light through the lens 302, and then light with a specified wavelength range is screened out through the light source filter 303.

Examples

Referring to fig. 1, 2, 11 and 12, the camera imaging assembly includes an industrial camera 20, a camera support 21 disposed on the main body 1, and an objective lens 22 matched with the industrial camera 20, wherein the objective lens 22 is disposed towards the light transmission detection position 101, six camera filters 23 are disposed between the objective lens 22 and the industrial camera 20, and a slicing mechanism for controlling different camera filters 23 to switch between the objective lens 22 and the industrial camera 20. The camera optical filters 23 corresponding to the used sample characteristics and the selection of the excitation light sources with different bottoms can be selected, the different camera optical filters 23 are controlled to move between the objective lens 22 and the industrial camera 20 through the slicing mechanism, the control is more convenient, the full-automatic switching is realized, the manual control is not needed, and the degree of automation is higher.

Referring to fig. 11 and 12, the slicing mechanism includes a slicing housing 24 disposed between the objective lens 22 and the industrial camera 20, a slicing turntable 25 disposed in the slicing housing 24, and a slicing motor 25 for controlling the rotation of the slicing turntable 25, wherein the camera optical filter 23 is fixedly disposed on the slicing turntable 25 in a circumferential manner around the rotation axis of the slicing turntable 25; the light source motor 32 drives the slice turntable 25 to rotate to control any one of the camera filters 23 to move between the objective lens 22 and the industrial camera 20. The slicing motor 25 is rotated to control the different camera filters 23 to move between the objective lens 22 and the industrial camera 20, so that the control is convenient, the camera filters 23 are detachably arranged in the slicing turntable 25, and the slicing turntable can be replaced according to requirements, so that the applicability is improved.

Referring to fig. 1 and 2, the camera support 21 is further provided with a focusing assembly, the focusing assembly includes a camera support plate 26 slidably disposed on the camera support 21, a camera screw mechanism 27 for driving the camera support plate 26 to translate away from or close to the light transmission detection position 101, and a camera lifting motor 28 for driving the camera screw mechanism 27, and the objective lens 22 and the industrial camera 20 are fixedly disposed on the camera support plate 26. The focusing assembly can drive the objective lens 22 and the industrial camera 20 to move up and down, so that the distance between the objective lens 22 and the industrial camera 20 and the counting plate is adjusted, the focusing function is realized, the focusing is more convenient, and various shooting requirements are met.

Preferably, a lens fixing cylinder 29 is further disposed between the slice housing 24 and the objective lens 22, the lens fixing cylinder 29 is fixedly connected with the bottom of the slice housing 24, the objective lens 22 is fixedly disposed on the lens fixing cylinder 29, and the camera support plate 26 is fixedly connected with the lens fixing cylinder 29.

Referring to fig. 1, 2, 3, 7 and 8, the moving assembly includes a counting assembly screw structure 130, and a screw control motor 131 for driving the counting assembly screw structure 130 to rotate, a screw nut 132 is disposed on the counting assembly screw structure 130, and the tray 11 is fixedly connected with the screw nut 132.

Referring to fig. 9 and 10, clamping blocks 112 are respectively disposed at two sides of the fixing groove 111, a clamping groove 1121 adapted to the counting plate 12 is disposed on the clamping block 112, one side of the clamping block 112 is fixedly connected with the tray 11 through an elastic member, and the elastic member is used for driving the clamping block 112 to translate towards the other clamping block 112. The counting plate 12 is fixed between the clamping blocks 112 at two sides during installation, the counting plate 12 is clamped and fixed through the elastic piece on one side of the clamping block 112, the fixing stability of the counting plate 12 is improved, and the counting plate is not easy to loosen.

Referring to fig. 10, the elastic member includes a connecting rod 1122 and a spring 1123, the connecting rod 1122 is connected to the clamping block 112 through the side edge of the tray 11, the connecting rod 1122 is slidably connected to the tray 11, and the spring 1123 is sleeved on the connecting rod 1122 and is used for driving the clamping block 112 to translate toward the other clamping block 112. The connecting rod 1122 is used to connect the clamp block 112 and define the direction of movement of the clamp block, and the spring 1123 is used to provide a spring force.

Referring to fig. 9 and 10, a micro switch 113 matched with the counting plate 12 is disposed in the fixing groove 111, and the micro switch 113 is used for monitoring whether the counting plate 12 is installed in place. The counting plate 12 is convenient to position, when the counting plate 12 is installed in place, the micro switch 113 can sense the counting plate 12, and the fluorescent cell counter can judge that the counting plate 12 is installed in place.

Referring to fig. 13, the counting plate 12 is provided with 8 counting cells 121, the counting plate 12 includes a glass layer 122, a glue layer 123, and a PVC sheet 124 sequentially disposed from bottom to top, the counting cells 121 are disposed on the glue layer 123 between the glass layer 122 and the PVC sheet 124, and the PVC sheet 124 is provided with an air vent 1241 and a sample feeding hole 1242 respectively communicating with two ends of the counting cells 121. Compared with the traditional counting plate, the counting plate increases the number of the counting cells 121, improves the detection efficiency, reduces the detection cost and can improve the efficiency of sample adding operation.

Preferably, the glass layer 122 is ultra-white glass with the thickness of 0.8-1.2 mm, the adhesive layer 123 is a double-sided adhesive layer with the thickness of 0.1-0.2 mm, the thickness of the PVC sheet 124 is 0.2-0.8 mm, the outer dimensions of the three layers are 95mm X25.4mm, and the distance between the counting ponds 121 is the same as that of the deep hole plates and is 9mm.

The working process of the utility model comprises the following steps:

In the working process of the light source component of the cell counter, various samples to be detected are respectively dripped into each counting pool 121 of the counting plate 12, then the counting plate 12 is fixed on a fixed groove 111 of the tray 11, the counting pool 121 to be detected is controlled to move to the light transmission detection position 101 by a moving component, a light source motor 32 drives a light source turntable 31 to rotate so as to control any one of the excitation light source 30 or the bright field light source 40 to project light beams to the light transmission detection position 101, and the excitation light source 30 rotates to the light transmission detection position 101 so as to be matched with the counting plate 12 on the tray 11, so that an excitation light source is provided for fluorescent dyes and the dyes emit fluorescence; the bright field light source 40 rotates to the light transmission detection position 101 to provide illumination for the counting plate 12 in a bright field detection mode; the various image signals generated on the cytometer plate are collected by a camera imaging assembly.

Standard parts used in the document of the application can be purchased from the market, the specific connection modes of all parts adopt conventional means such as mature bolts, rivets and welding in the prior art, the electric sliding rail sliding seat, the air cylinder, the welding machine, the electric telescopic rod and the internal parts of the controller adopt conventional models in the prior art, the internal structure of the electric sliding rail sliding seat, the air cylinder, the welding machine, the electric telescopic rod and the controller belong to the prior art structure, a worker can finish normal operation of the electric sliding rail sliding seat, the electric telescopic rod and the controller according to the manual of the prior art, and the circuit connection adopts the conventional connection modes in the prior art, so that the specific description is not made.

It should be noted that, although the foregoing embodiments have been described herein, the scope of the present utility model is not limited thereby. Therefore, based on the innovative concepts of the present utility model, alterations and modifications to the embodiments described herein, or equivalent structures or equivalent flow transformations made by the present description and drawings, apply the above technical solutions directly or indirectly to other relevant technical fields, all of which are included in the scope of protection of the present patent.

Claims

1. A cytometer light source assembly comprising an excitation light source (30) and a bright field light source (40), characterized in that: the light source rotating disk (31) and a motor (32) for driving the light source rotating disk (31) to rotate are also included, the excitation light source (30) and the bright field light source (40) are fixedly arranged on the light source rotating disk (31) in a circumferential mode around the rotation axis of the light source rotating disk (31), and at least two excitation light sources (30) are arranged;

At least one point on the upper side or the lower side of the light source turntable (31) is used as a detection point, and the motor (32) drives the light source turntable (31) to rotate so as to control any one of the excitation light source (30) or the bright field light source (40) to project light beams to the detection point.

2. A cytometer light source assembly as described in claim 1, wherein: the light source turntable (31) is also provided with a plurality of light source lamp holders (33), and the excitation light source (30) is obliquely arranged on the light source lamp holders (33).

3. A cytometer light source assembly as described in claim 2 wherein: each light source lamp holder (33) is respectively provided with two excitation light sources (30), and light beams projected by the excitation light sources (30) on the same light source lamp holder (33) can form an intersection at the detection point.

4. A cytometer light source assembly as described in claim 3 wherein: the light source lamp bracket (33) is provided with a Y-shaped bracket (331), and the two excitation light sources (30) are respectively and oppositely arranged at two sides of the Y-shaped bracket (331).

5. A cytometer light source assembly as described in claim 2 wherein: the excitation light source (30) comprises an LED lamp panel (301), a lens (302) for adjusting the light of the LED lamp panel (301) into parallel light, and a light source filter (303) for filtering light in a non-specified wavelength range.