CN221051882U - Plant cell secondary metabolite enrichment optimizing culture device - Google Patents
Plant cell secondary metabolite enrichment optimizing culture device Download PDFInfo
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- CN221051882U CN221051882U CN202322625839.1U CN202322625839U CN221051882U CN 221051882 U CN221051882 U CN 221051882U CN 202322625839 U CN202322625839 U CN 202322625839U CN 221051882 U CN221051882 U CN 221051882U
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- 229930000044 secondary metabolite Natural products 0.000 title claims abstract description 25
- 238000003756 stirring Methods 0.000 claims description 56
- 238000006243 chemical reaction Methods 0.000 claims description 40
- 230000007246 mechanism Effects 0.000 claims description 29
- 238000010008 shearing Methods 0.000 abstract description 19
- 238000004113 cell culture Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 238000005457 optimization Methods 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
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- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
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- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 229930014626 natural product Natural products 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a plant cell secondary metabolite enrichment optimizing culture device, which aims to solve the technical problem that the production amount of secondary metabolites is small due to large shearing force of the current plant cell culture device.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to an enrichment and optimization culture device for secondary metabolites of plant cells.
Background
Plant tissue culture is a technique that utilizes plant cells, tissues or organs for cultivation and propagation under sterile conditions. The technology can be used for plant propagation, breeding, disease life detection, gene transformation and other aspects, and can also be applied to the production of secondary metabolites. Secondary metabolites are a class of compounds produced by plants during the growth process, which are not necessary for plant growth and development, but have important application values such as medicines, fragrances, pigments and the like. The plant tissue culture can promote the production of secondary metabolites of plant cells or tissues by means of regulating the components of a culture medium, adding hormone, a permeabilizer and the like.
The plant cell culture has the characteristics of long period, weak cell shearing resistance, easy agglomeration and the like; meanwhile, the purpose of plant cell-scale culture is to produce natural products, which are cell growth metabolites. Therefore, the design of the plant cell culture reactor is considered to be advantageous not only for cell growth but also for accumulation and separation of the product. The shearing force generated by the culture device is large, so that cells are easy to damage, the growth and metabolism of the cells are directly influenced, and the secondary product generation is extremely influenced. The stirring mechanism in the culture device has the function of uniformly mixing the later added fluid, and the enrichment is to mix two liquids which are incompatible or have a relatively slow compatible speed, so that one liquid phase is uniformly dispersed into the other liquid phase in the form of tiny liquid drops, and the stirring mechanism plays an important role in the culture of plant cells in the device, so that the shearing force generated by the stirring mechanism needs to be effectively reduced, and the uniform liquid is needed, and in view of the problem, the invention provides a plant cell secondary metabolite enrichment optimizing culture device.
Disclosure of utility model
The utility model aims to overcome the defects of the prior art, adapt to the actual needs, and provide an enrichment and optimization culture device for secondary metabolites of plant cells, so as to solve the technical problem of small production amount of the secondary metabolites caused by large shearing force of the current plant cell culture device.
In order to achieve the purpose of the utility model, the technical scheme adopted by the utility model is as follows: the plant cell secondary metabolite enrichment optimizing culture device comprises a base, wherein a pulley is arranged at the bottom end of the base, a support column is arranged on the base, a reaction tank body is arranged on the support column, and a stirring mechanism is arranged in the reaction tank body; the stirring mechanism comprises a servo motor, a rotating shaft and a plurality of stirring impellers, the servo motor is arranged at the top end of the reaction tank body, the bottom end of the servo motor is connected with the top end of the rotating shaft, and the stirring impellers are sleeved on the rotating shaft; the stirring impeller comprises a connecting hole, a plurality of fan blades, a plurality of wheel shafts and a plurality of propeller blades, wherein the connecting hole is sleeved on the rotating shaft, the fan blades are connected to the outer side of the connecting hole, the wheel shafts are arranged on the fan blades, and the propeller blades are rotatably connected with the fan blades through the wheel shafts; a plurality of through holes are also formed on the fan blades.
Preferably, the top end of the reaction tank body is provided with a stirring hole, an air inlet valve and an air outlet valve, the stirring mechanism is inserted into the reaction tank body through the stirring hole, and the air inlet valve and the air outlet valve are arranged on the upper part of the outer wall of the reaction tank body;
And a pressure sensor hole, a temperature sensor hole and a charging hole are formed in the bottom end of the reaction tank body.
Preferably, the side surface of the fan blade is in a round corner structure, and a plurality of fan blades are connected with the connecting holes in an inclined mode.
Compared with the prior art, the utility model has the beneficial effects that:
1. According to the utility model, the structure of the existing plant cell culture device is improved, the stirring mechanism is arranged in the reaction tank body, the servo motor on the stirring mechanism drives the rotating shaft to rotate, and the shearing force of the stirring mechanism is reduced by arranging the net-shaped through holes on the fan blades and the inclined arrangement of the fan blades, so that the plant cell secondary metabolite is not easy to damage cells in the culture device due to the small shearing force of the stirring mechanism, the generation amount of the secondary metabolite is improved, and the practical use efficiency is improved.
2. According to the utility model, the wheel shafts are arranged on the side surfaces of the fan blades, so that when the servo motor drives the rotating shaft to drive the stirring impeller to rotate, the propeller blades are rotationally connected to the wheel shafts, and rotate under the impact of water flow, so that the fluid in the reaction tank body is uniform while the shearing force is reduced, and the enrichment optimization of secondary metabolites is facilitated.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic cross-sectional view of a reaction tank and a stirring mechanism according to the present utility model;
FIG. 3 is a schematic view of a stirring impeller according to the present utility model;
In the figure: 1. a base; 2. a pulley; 3. a support column; 4. a reaction tank body; 5. a stirring mechanism;
401. Stirring holes; 402. an intake valve; 403. an exhaust valve; 404. a pressure sensor aperture; 405. a temperature sensor hole; 406. a feed hole;
501. A servo motor; 502. a rotating shaft; 503. a stirring impeller;
5031. a connection hole; 5032. a fan blade; 5033. a through hole; 5034. a wheel axle; 5035. a propeller blade.
Detailed Description
The utility model is further illustrated by the following examples in conjunction with the accompanying drawings:
Examples: the plant cell secondary metabolite enrichment optimizing culture device, see fig. 1-3, comprises a base 1, wherein a pulley 2 is arranged at the bottom end of the base 1, a support column 3 is arranged on the base 1, a reaction tank body 4 is arranged on the support column 3, and a stirring mechanism 5 is arranged in the reaction tank body 4; the stirring mechanism 5 comprises a servo motor 501, a rotating shaft 502 and a plurality of stirring impellers 503, wherein the servo motor 501 is arranged at the top end of the reaction tank body 4, the bottom end of the servo motor 501 is connected with the top end of the rotating shaft 502, and the stirring impellers 503 are sleeved on the rotating shaft 502; the stirring impeller 503 comprises a connecting hole 5031, a plurality of fan blades 5032, a plurality of wheel shafts 5034 and a plurality of propeller blades 5035, the connecting hole 5031 is sleeved on the rotating shaft 502, the fan blades 5032 are connected to the outer side of the connecting hole 5031, the wheel shafts 5034 are arranged on the fan blades 5032, and the propeller blades 5035 are rotatably connected with the fan blades 5032 through the wheel shafts 5034; the fan blades 5032 are further provided with a plurality of through holes 5033. According to the utility model, through improving the structure of the existing plant cell culture device, the stirring mechanism 5 is arranged in the reaction tank body 4, the servo motor 501 on the stirring mechanism 5 drives the rotating shaft 502 to drive the rotating shaft 502 to rotate, and the shearing force of the stirring mechanism 5 is reduced by arranging the reticular through holes 5033 on the fan blades 5032 and the inclined arrangement of the fan blades 5032, so that the plant cell secondary metabolite is not easy to damage cells in the culture device due to the small shearing force of the stirring mechanism 5, the generation amount of the secondary metabolite is improved, and the actual use efficiency is improved; the connecting holes 5031 of the stirring impeller 503 are used for being sleeved on the rotating shaft 502, the stirring impeller 503 is sleeved on the rotating shaft 502 to have three groups, and plays a role in preventing precipitation in the reaction tank 4 uniformly in the culture process, so that when the servo motor 501 drives the rotating shaft 502 to drive the stirring impeller 503 to rotate, the propeller 5035 is rotationally connected to the wheel shaft 5034, the propeller 5035 rotates under the impact of water flow, the shearing force is reduced, the fluid in the reaction tank 4 is uniform, and the enrichment and optimization of secondary metabolites are facilitated; the through holes 5033 formed on the fan 5032 are beneficial to reducing a certain shearing force in the stirring process, so that the possibility of crushing cells during stirring is reduced, and the output of secondary metabolites is increased.
Specifically, as shown in fig. 1, a stirring hole 401, an air inlet valve 402 and an air outlet valve 403 are provided at the top end of the reaction tank 4, the stirring mechanism 5 is inserted into the reaction tank 4 through the stirring hole 401, and the air inlet valve 402 and the air outlet valve 403 are provided at the upper part of the outer wall of the reaction tank 4; the bottom end of the reaction tank body 4 is provided with a pressure sensor hole 404, a temperature sensor hole 405 and a charging hole 406. In the utility model, a stirring hole 401 is formed in a reaction tank body 4 and used for inserting a stirring mechanism 5 into the reaction tank body 4, an air inlet valve 402 and an air outlet valve 403 are used for controlling the inlet and the outlet of gas in the reaction tank body 4, a pressure sensor hole 404 is used for accurately monitoring the pressure value in the reaction tank body 4, a temperature sensor hole 405 is arranged beside the pressure sensor hole 404 and used for monitoring the temperature in the reaction tank body 4, and a charging hole 406 is used for entering materials.
It should be noted that, as shown in fig. 3, the side surface of the fan 5032 is a rounded structure, and the fan 5032 is connected to the connecting hole 5031 in an inclined manner. According to the utility model, the side surface of the fan 5032 is provided with the round corner structure, so that when the servo motor 501 drives the rotating shaft 502 to drive the rotating shaft 502 to rotate, the shearing force of the fan 5032 is further reduced, the practical effect is better, the synthesis of secondary metabolites is facilitated, the fan 5032 is inclined mainly for reducing the shearing force, and plant cells are prevented from being crushed during horizontal prevention, so that the production efficiency is reduced.
Working principle: when the utility model is used, because the pulley 2 is arranged at the bottom end of the base 1, the support column 3 is arranged on the base 1, the reaction tank 4 is arranged on the support column 3, the stirring mechanism 5 is arranged in the reaction tank 4, the stirring hole 401 is arranged on the reaction tank 4 for the stirring mechanism 5 to be inserted into the reaction tank 4, the air inlet valve 402 and the air outlet valve 403 are used for controlling the inlet and the outlet of the air in the reaction tank 4, the pressure sensor hole 404 is used for accurately monitoring the pressure value in the reaction tank 4, the temperature sensor hole 405 is arranged beside the pressure sensor hole 404 and used for monitoring the internal temperature of the reaction tank 4, the charging hole 406 is used for the inlet of materials, the connecting hole 5031 of the stirring impeller 503 is used for being sleeved on the rotating shaft 502, the stirring impeller 503 is sleeved on the rotating shaft 502 for three groups, the stirring impeller 503 plays a role of uniformly preventing sedimentation in the culture process, the through hole 5033 arranged on the impeller 5032 is beneficial to reducing a certain shearing force in the stirring process, reducing the possibility of cells being broken in the stirring process, increasing the output of secondary metabolic products, the impeller 5032 is mainly inclined, the impeller 5035 is mainly used for reducing the shearing force of the impeller 5032, and the impeller 5032 is also used for reducing the shearing force in order to drive the impeller 5035 to drive the rotating shaft 5035 to further evenly, and reducing the shearing force in order to drive the rotating shaft 5035 to further evenly, and reducing the shearing force in the rotating mechanism by the rotating mechanism is more than the rotating impeller 5035, and the rotating mechanism is more than the rotating shaft 5035, and the impeller is in order to drive the rotating mechanism is more evenly and the rotating and the impeller (35) and the impeller) to be in the rotating efficiency, is favorable for the enrichment optimization of secondary metabolites.
The embodiments of the present utility model are disclosed as preferred embodiments, but not limited thereto, and those skilled in the art will readily appreciate from the foregoing description that various modifications and variations can be made without departing from the spirit of the present utility model.
Claims (3)
1. The plant cell secondary metabolite enrichment optimizing culture device is characterized by comprising a base (1), wherein a pulley (2) is arranged at the bottom end of the base (1), a support column (3) is arranged on the base (1), a reaction tank body (4) is arranged on the support column (3), and a stirring mechanism (5) is arranged in the reaction tank body (4);
the stirring mechanism (5) comprises a servo motor (501), a rotating shaft (502) and a plurality of stirring impellers (503), wherein the servo motor (501) is arranged at the top end of the reaction tank body (4), the bottom end of the servo motor (501) is connected with the top end of the rotating shaft (502), and the stirring impellers (503) are sleeved on the rotating shaft (502);
The stirring impeller (503) comprises a connecting hole (5031), a plurality of fan blades (5032), a plurality of wheel shafts (5034) and a plurality of propeller blades (5035), wherein the connecting hole (5031) is sleeved on the rotating shaft (502), the fan blades (5032) are connected to the outer side of the connecting hole (5031), the wheel shafts (5034) are arranged on the fan blades (5032), and the propeller blades (5035) are rotationally connected with the fan blades (5032) through the wheel shafts (5034);
A plurality of through holes (5033) are also formed in the fan blades (5032).
2. The plant cell secondary metabolite enrichment optimizing culture device according to claim 1, wherein a stirring hole (401), an air inlet valve (402) and an air outlet valve (403) are arranged at the top end of the reaction tank body (4), the stirring mechanism (5) is inserted into the reaction tank body (4) through the stirring hole (401), and the air inlet valve (402) and the air outlet valve (403) are arranged at the upper part of the outer wall of the reaction tank body (4);
The bottom end of the reaction tank body (4) is provided with a pressure sensor hole (404), a temperature sensor hole (405) and a charging hole (406).
3. The plant cell secondary metabolite enrichment optimizing culture device according to claim 1, wherein the side surface of each fan blade (5032) is in a round corner structure, and a plurality of the fan blades (5032) are connected with the connecting holes (5031) in an inclined mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322625839.1U CN221051882U (en) | 2023-09-27 | 2023-09-27 | Plant cell secondary metabolite enrichment optimizing culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322625839.1U CN221051882U (en) | 2023-09-27 | 2023-09-27 | Plant cell secondary metabolite enrichment optimizing culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221051882U true CN221051882U (en) | 2024-05-31 |
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ID=91223836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322625839.1U Active CN221051882U (en) | 2023-09-27 | 2023-09-27 | Plant cell secondary metabolite enrichment optimizing culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221051882U (en) |
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2023
- 2023-09-27 CN CN202322625839.1U patent/CN221051882U/en active Active
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