CN220887562U - Device for suspension culture of plant cells - Google Patents
Device for suspension culture of plant cells Download PDFInfo
- Publication number
- CN220887562U CN220887562U CN202322396629.XU CN202322396629U CN220887562U CN 220887562 U CN220887562 U CN 220887562U CN 202322396629 U CN202322396629 U CN 202322396629U CN 220887562 U CN220887562 U CN 220887562U
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- China
- Prior art keywords
- base
- pulsator
- impeller
- rotating shaft
- stirring mechanism
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- 238000004114 suspension culture Methods 0.000 title claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 34
- 238000012258 culturing Methods 0.000 claims description 7
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 15
- 238000010008 shearing Methods 0.000 abstract description 7
- 230000034994 death Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 18
- 238000004113 cell culture Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000030833 cell death Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a device for suspension culture of plant cells, which aims to solve the technical problems of cell disruption and death caused by stirring of the current device for suspension culture of plant cells, and comprises a base, wherein the bottom end of the base is provided with a pulley, the base is provided with a plurality of support columns, and a culture tank is arranged on each support column; the culture tank comprises a base, a stirring mechanism and a shell, wherein the structure of the existing suspension culture plant cell device is improved, the stirring mechanism is arranged on the base of the culture tank, a rotating shaft is used for connecting a pulsator A on the stirring mechanism, a top cover is arranged on the twisting blade of the pulsator A, the shearing force generated by rotation of the pulsator A is greatly reduced, and the top cover enables a liquid sealing layer not to enter a rotating cavity of the pulsator A, so that the phenomenon of death and breakage of plant cells caused by the shearing force generated by rotation of the twisting blade is prevented, and the actual culture efficiency is improved.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a device for suspension culture of plant cells.
Background
In recent years, the availability of wild medicinal plant resources has decreased year by year due to deterioration of the ecological environment and excessive harvesting of wild plants. Therefore, accelerating the breeding of excellent plant materials and the development of natural medicines and protecting and utilizing rare plant resources are always the research focus of plant cell culture work. In order to produce natural plant source functional components on a large scale or to carry out large-scale breeding of plant materials, the plant cell culture device which meets the market demand and can break the condition restrictions of regional climate and the like has very important functions. Plant cell culture is a plant tissue culture method performed in an isolated condition in a single cell or a cell mass. The technology is not influenced by space-time, external natural environment, character separation and the like, and can culture uniform and consistent cells in a large scale in a short time, thereby being convenient for subsequent research and utilization.
Compared with animal cells, the plant cells can grow in suspension, and are suitable for large-scale culture. However, plant cells have lower metabolic activity, and the synthesis and accumulation speeds of secondary substances are slower, so that compared with microbial cell culture, the difficulty is higher, and a stirring mechanism is required to fuse the later-added hormone or growth agent with liquid. The existing device for culturing plant cells in a suspension mode generally adopts a stirring type culture method, but plant cells have cell walls with different thicknesses and containing cellulose, the tensile strength is higher, so that the device is more sensitive to shearing force, the growth of the cells can be obviously reduced under the stirring of a stirring mechanism, and the death and crushing phenomena can occur due to the rotation of paddles, so that the efficiency of culturing the plant cells is greatly reduced.
Disclosure of utility model
The utility model aims to overcome the defects of the prior art, adapt to the actual needs, and provide a device for suspending and culturing plant cells, so as to solve the technical problems of cell disruption and cell death caused by stirring of the current device for suspending and culturing plant cells.
In order to achieve the purpose of the utility model, the technical scheme adopted by the utility model is as follows: the device for suspending and culturing plant cells comprises a base, wherein pulleys are arranged at the bottom end of the base, a plurality of support columns are arranged on the base, and culture tanks are arranged on the support columns; the culture tank comprises a base, a stirring mechanism and a shell, wherein the base is arranged at the top ends of a plurality of support columns, the stirring mechanism is arranged on the base, and the shell is arranged on the base.
Preferably, a connecting hole A is formed in the base, a rotating shaft penetrates through the connecting hole A, a servo motor is connected to the bottom end of the rotating shaft, and the top end of the rotating shaft is connected with the stirring mechanism.
Preferably, the stirring mechanism comprises a pulsator A and a pulsator B, wherein the pulsator A is arranged at the top end of the base, the pulsator B is arranged at the top end of the pulsator A, and the rotating shaft is rotationally connected with the pulsator A and the pulsator B.
Preferably, the impeller a comprises a chassis, a connecting hole B, a plurality of torsion blades and a top cover, wherein the connecting hole B is formed in the chassis, the torsion blades are obliquely arranged on the top surface of the chassis, and the top cover is arranged on the top end of the chassis; the rotating shaft penetrates through the connecting hole B.
Preferably, the impeller B includes a bottom shaft and an impeller, the bottom shaft is rotatably connected to the top end of the rotating shaft, and the bottom end of the impeller is connected to the top end of the bottom shaft.
Preferably, the impeller is petal-shaped.
Compared with the prior art, the utility model has the beneficial effects that:
1. According to the utility model, the structure of the existing suspension culture plant cell device is improved, the stirring mechanism is arranged on the culture tank base, the rotating shaft is used for connecting the impeller A on the stirring mechanism, the torsion blades of the impeller A are arranged to be inclined, the top cover is arranged on the torsion blades, so that the shearing force generated by rotation of the impeller A is greatly reduced, and the top cover enables the liquid sealing layer not to enter the rotation cavity of the impeller A, so that the phenomenon of plant cell death and breakage caused by the shearing force generated by rotation of the torsion blades is prevented, and the practical culture efficiency is improved.
2. According to the utility model, the impeller B is arranged at the top end of the rotating shaft, and the impeller B which is in petal-shaped spiral ascending is used for enabling the servo motor to drive the rotating shaft to drive the impeller A to generate vortex which is amplified to a certain extent through the structural shape of the impeller B, so that the effect of fusing later added liquid medicine and liquid is achieved, and the uniform effect is enhanced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic side view of the present utility model;
FIG. 3 is a schematic diagram of the medium culture tank structure of the present utility model;
FIG. 4 is a schematic view of a base and stirring mechanism according to the present utility model;
FIG. 5 is a schematic diagram of a split structure of the middle base and stirring mechanism of the utility model;
In the figure: 1. a base; 2. a pulley; 3. a support column; 4. a culture tank;
401. A base; 402. a stirring mechanism; 403. a housing; 404. a connecting hole A; 405. a rotating shaft; 406. a servo motor; 407. a wave wheel A; 408. a wave wheel B;
4071. a chassis; 4072. a connecting hole B; 4073. twisting the blade; 4074. a top cover;
4081. A bottom shaft; 4082. an impeller.
Detailed Description
The utility model is further illustrated by the following examples in conjunction with the accompanying drawings:
examples: the device for suspending and culturing plant cells comprises a base 1, wherein a pulley 2 is arranged at the bottom end of the base 1, a plurality of support columns 3 are arranged on the base 1, and a culture tank 4 is arranged on each support column 3; the utility model relates to a culture tank 4, which comprises a base 401, a stirring mechanism 402 and a shell 403, wherein the base 401 is arranged at the top ends of a plurality of support columns 3, the stirring mechanism 402 is arranged on the base 401, the shell 403 is arranged on the base 401, the structure of the existing suspension culture plant cell device is improved, the stirring mechanism 402 is arranged on the base of the culture tank 4, a rotating shaft 405 is used for connecting a pulsator A407 on the stirring mechanism 402, a torsional blade 4073 of the pulsator A407 is arranged in an inclined shape, a top cover 4074 is also arranged on the torsional blade 4073, the shearing force generated by the rotation of the pulsator A407 is greatly reduced, and the top cover 4074 enables a liquid sealing layer to not enter a rotating cavity of the pulsator A407, so that the phenomenon of plant cell death and breakage caused by the shearing force generated by the rotation of the torsional blade 4073 is prevented, and the actual culture efficiency is improved.
Specifically, as shown in fig. 3 to 5, a connection hole a404 is formed in the base 401, a rotating shaft 405 is disposed on the connection hole a404 in a penetrating manner, a servo motor 406 is connected to the bottom end of the rotating shaft 405, and the stirring mechanism 402 is connected to the top end of the rotating shaft 405. In the utility model, a connecting hole A404 formed on a base 401 is used for penetrating a rotating shaft 405, the bottom end of the rotating shaft 405 is connected with a servo motor 406, and the servo motor 406 drives a stirring mechanism 402 to rotate so as to uniformly stir liquid in a culture tank 4.
Further, referring to fig. 3 to 5, the stirring mechanism 402 includes a pulsator a407 and a pulsator B408, the pulsator a407 is disposed at the top end of the base 401, the pulsator B408 is disposed at the top end of the pulsator a407, and the rotating shaft 405 is rotatably connected to the pulsator a407 and the pulsator B408. In the utility model, a stirring mechanism 402 consists of a pulsator A407 and a pulsator B408, wherein the pulsator A407 is arranged on a base 401, the pulsator B408 is arranged above the pulsator A407, and a servo motor 406 drives a rotating shaft 405 to rotate so as to drive the pulsator A407 and the pulsator B408 to rotate.
Still further, as shown in fig. 5, the pulsator a407 includes a chassis 4071, a connection hole B4072, a plurality of torsion blades 4073 and a top cover 4074, the connection hole B4072 is opened on the chassis 4071, the torsion blades 4073 are obliquely arranged on the top surface of the chassis 4071, and the top cover 4074 is arranged on the top end of the chassis 4071; the rotating shaft 405 is disposed through the connecting hole B4072. The impeller A407 in the utility model is composed of a chassis 4071, a connecting hole B4072, a plurality of torsion blades 4073 and a top cover 4074, wherein the bottom end of the chassis 4071 is connected with a base 401, the torsion blades 4073 are arranged on the chassis 4071 by taking the connecting hole B4072 as an axle center, and five groups of the torsion blades are arranged on the chassis 4071, and the top cover 4074 is matched with the chassis 4071 in an installation way to form the impeller A407.
It should be noted that, referring to fig. 5, the impeller B408 includes a bottom shaft 4081 and an impeller 4082, the bottom shaft 4081 is rotatably connected to the top end of the rotating shaft 405, and the bottom end of the impeller 4082 is connected to the top end of the bottom shaft 4081. In the utility model, the impeller B408 consists of the bottom shaft 4081 and the impeller 4082, the bottom end of the bottom shaft 4081 is rotationally connected with the top end of the rotating shaft 405, when the servo motor 406 drives the rotating shaft 405 to rotate to drive the impeller A407, the torsion blades 4073 of the impeller A407 enable liquid to form vortex flow to come out through the top cover 4074, and the impeller 4082 is rotated due to the rotational connection of the bottom shaft 4081 and the rotating shaft 405, so that the liquid at the upper part of the culture tank 4 is uniformly acted.
It is noted that, as shown in fig. 4 and fig. 5, the impeller 4082 is petal-shaped, and the impeller B408 is arranged at the top end of the rotating shaft 405, and the impeller B408 spirally rises in petal shape, so that the vortex generated by the rotating shaft 405 driven by the servo motor 406 to drive the impeller a407 is amplified to a certain extent through the structural shape of the impeller B408, thereby playing the role of fusing the later added liquid medicine and liquid and enhancing the uniformity effect.
Working principle: when the utility model is used, the connecting hole A404 arranged on the base 401 is used for penetrating the rotating shaft 405, the bottom end of the rotating shaft 405 is connected with the servo motor 406, the servo motor 406 drives the stirring mechanism 402 to rotate so as to uniformly stir the liquid in the culture tank 4, the stirring mechanism 402 consists of the impeller A407 and the impeller B408, the impeller A407 is arranged on the base 401, the impeller B408 is arranged above the impeller A407, the servo motor 406 drives the rotating shaft 405 to rotate so as to drive the impeller A407 and the impeller B408 to rotate, the impeller A407 consists of the base 4071, the connecting hole B4072, a plurality of torsion blades 4073 and a top cover 4074, the bottom end of the base 4071 is connected with the base 401, the torsion blades 4073 are arranged on the base 4071 in five groups by taking the connecting hole B4072 as an axle center, the top cover 4071 is assembled and matched with the base 4071 to form the impeller A407, the impeller B408 comprises a bottom shaft 4081 and an impeller 4082, the bottom end of the bottom shaft 4081 is rotationally connected with the top end of a rotating shaft 405, when a servo motor 406 drives the rotating shaft 405 to rotate so as to drive the impeller A407, the torsion blades 4073 of the impeller A407 enable liquid to form vortex flow to come out through a top cover 4074, the impeller 4082 is rotated by the vortex flow due to the fact that the bottom shaft 4081 is rotationally connected with the rotating shaft 405, the impeller B408 is arranged at the top end of the rotating shaft 405, the impeller B408 ascends in a petal-shaped spiral mode, the vortex flow generated by the impeller A407 is driven by the servo motor 406 to be amplified to a certain extent through the structural shape of the impeller B408, and therefore the later added liquid medicine and liquid are fused, and the uniform effect is enhanced.
The embodiments of the present utility model are disclosed as preferred embodiments, but not limited thereto, and those skilled in the art will readily appreciate from the foregoing description that various modifications and variations can be made without departing from the spirit of the present utility model.
Claims (4)
1. The device for suspending and culturing plant cells is characterized by comprising a base (1), wherein a pulley (2) is arranged at the bottom end of the base (1), a plurality of support columns (3) are arranged on the base (1), and a culture tank (4) is arranged on each support column (3);
The culture tank (4) comprises a base (401), a stirring mechanism (402) and a shell (403), wherein the base (401) is arranged at the top ends of a plurality of support columns (3), the stirring mechanism (402) is arranged on the base (401), and the shell (403) is arranged on the base (401);
A connecting hole A (404) is formed in the base (401), a rotating shaft (405) is arranged on the connecting hole A (404) in a penetrating mode, a servo motor (406) is connected to the bottom end of the rotating shaft (405), and the top end of the rotating shaft (405) is connected with the stirring mechanism (402);
The stirring mechanism (402) comprises a pulsator A (407) and a pulsator B (408), wherein the pulsator A (407) is arranged at the top end of the base (401), the pulsator B (408) is arranged at the top end of the pulsator A (407), and the pulsator A (407) and the pulsator B (408) are rotatably connected through a rotating shaft (405).
2. The device for suspension culture of plant cells according to claim 1, wherein the pulsator a (407) comprises a chassis (4071), a connecting hole B (4072), a plurality of torsion blades (4073) and a top cover (4074), wherein the connecting hole B (4072) is opened on the chassis (4071), the torsion blades (4073) are obliquely arranged on the top surface of the chassis (4071), and the top cover (4074) is arranged on the top end of the chassis (4071);
The rotating shaft (405) penetrates through the connecting hole B (4072).
3. The device for suspension culture of plant cells according to claim 2, wherein the pulsator B (408) comprises a bottom shaft (4081) and an impeller (4082), the bottom shaft (4081) is rotatably connected to the top end of the rotating shaft (405), and the bottom end of the impeller (4082) is connected to the top end of the bottom shaft (4081).
4. A device for suspension culture of plant cells according to claim 3, characterized in that the impeller (4082) is petal-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322396629.XU CN220887562U (en) | 2023-09-05 | 2023-09-05 | Device for suspension culture of plant cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322396629.XU CN220887562U (en) | 2023-09-05 | 2023-09-05 | Device for suspension culture of plant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220887562U true CN220887562U (en) | 2024-05-03 |
Family
ID=90875400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322396629.XU Active CN220887562U (en) | 2023-09-05 | 2023-09-05 | Device for suspension culture of plant cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220887562U (en) |
-
2023
- 2023-09-05 CN CN202322396629.XU patent/CN220887562U/en active Active
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