CN220988954U - Rat middle cerebral artery line thrombus device - Google Patents
Rat middle cerebral artery line thrombus device Download PDFInfo
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- CN220988954U CN220988954U CN202322649846.5U CN202322649846U CN220988954U CN 220988954 U CN220988954 U CN 220988954U CN 202322649846 U CN202322649846 U CN 202322649846U CN 220988954 U CN220988954 U CN 220988954U
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- cerebral artery
- middle cerebral
- bolt
- pipe
- rat
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- 210000003657 middle cerebral artery Anatomy 0.000 title claims abstract description 19
- 208000007536 Thrombosis Diseases 0.000 title claims abstract description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000741 silica gel Substances 0.000 claims description 9
- 229910002027 silica gel Inorganic materials 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 7
- 210000000269 carotid artery external Anatomy 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 9
- 208000027418 Wounds and injury Diseases 0.000 abstract description 4
- 210000001627 cerebral artery Anatomy 0.000 abstract description 3
- 230000006378 damage Effects 0.000 abstract description 3
- 208000014674 injury Diseases 0.000 abstract description 2
- 241000700159 Rattus Species 0.000 description 17
- 210000001168 carotid artery common Anatomy 0.000 description 13
- 210000004004 carotid artery internal Anatomy 0.000 description 11
- 210000001367 artery Anatomy 0.000 description 8
- 230000000903 blocking effect Effects 0.000 description 7
- 201000006474 Brain Ischemia Diseases 0.000 description 6
- 206010008120 Cerebral ischaemia Diseases 0.000 description 6
- 210000001715 carotid artery Anatomy 0.000 description 6
- 206010008118 cerebral infarction Diseases 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 4
- 230000036770 blood supply Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 208000005189 Embolism Diseases 0.000 description 2
- 206010019468 Hemiplegia Diseases 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000002490 cerebral effect Effects 0.000 description 2
- 230000009193 crawling Effects 0.000 description 2
- 210000003195 fascia Anatomy 0.000 description 2
- 230000000762 glandular Effects 0.000 description 2
- 210000004283 incisor Anatomy 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010033675 panniculitis Diseases 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000009958 sewing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 210000004304 subcutaneous tissue Anatomy 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000003106 tissue adhesive Substances 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 206010070693 Vascular dissection Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 238000007428 craniotomy Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000010410 reperfusion Effects 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
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- Materials For Medical Uses (AREA)
Abstract
The utility model discloses a rat middle cerebral artery thrombus device, which comprises a catheter and a thrombus; the guide pipe is of a Y-shaped structure and comprises a main pipe and a branch pipe communicated with the main pipe; the wire bolt enters the main pipeline from the branch pipeline. The middle rat cerebral artery thrombus device has a simple structure, is convenient to operate, does not need to cut off external carotid arteries and branches thereof in the use process, reduces operation injury, causes the thrombus to be more similar to a real state, and simultaneously reduces the experience skill requirements on ligation and operation of operators.
Description
Technical Field
The utility model belongs to the technical field of cerebral ischemia model preparation, and particularly relates to a rat middle cerebral artery thrombus device.
Background
Currently, a variety of experimental animals have been used to prepare models of cerebral ischemia, including rodents, non-human primates, rabbits, pigs, and the like. Since rat cerebral vascular dissection and neuron approach to higher animals, reproducibility is good, cost is low, and since brain tissue is small, biochemical detection is convenient after freezing, so that a model of cerebral ischemia is prepared from rats in recent years.
The cerebral ischemia model is generally a cerebral middle artery embolism model widely accepted internationally, and the classical method of the model is a wire embolism method: the exposed neck blood vessels were separated, nylon wires were inserted from the bifurcation of the external carotid artery (external carotid artery, ECA) or the common carotid artery (common carotid artery, CCA), into the internal carotid artery (internal carotid artery, ICA), blocking the blood supply to the middle cerebral artery (Middle Cerebral Artery, MCA) and all its side branches, resulting in focal ischemia of the MCA region.
The wire bolt method has the advantages that craniotomy is not needed for model manufacture, and the wound is small; the operation is relatively simple, the ischemic part is relatively fixed, and the controllability is good; can realize reperfusion after cerebral ischemia, and is an ideal standard focal cerebral ischemia animal model. When the suture is used, the external carotid artery is required to be separated, the branches cutting the external carotid artery are ligatured, the artery is cut between two ligature points, and certain experience skills are required for ligation and operation, so that certain damage can be caused.
Disclosure of utility model
The utility model aims to solve the technical problem of providing a rat middle cerebral artery thrombus device, which reduces operation injury, is convenient to operate and improves model success rate.
In order to solve the technical problems, the utility model adopts the following technical scheme: a rat middle cerebral artery thrombus device, which comprises a catheter and a thrombus; the guide pipe is of a Y-shaped structure and comprises a main pipe and a branch pipe communicated with the main pipe; the wire bolt enters the main pipeline from the branch pipeline.
Further, the plug includes a main body, a head end blocking portion, and a tail end blocking portion.
Further, the head end plugging part and the tail end plugging part are solid silica gel bodies, and the solid silica gel bodies are cylindrical structures.
Further, the diameters of the solid silica gel bodies of the head end plugging part and the tail end plugging part of the wire plug are +/-0.35 mm, and the distance between the head end and the tail end is +/-20 mm.
Further, the head end plugging part and the tail end plugging part can be plugging air bags arranged on the peripheries of the head end and the tail end of the wire bolt main body respectively, an air passage communicated with the air bag plugging air bags is arranged in the main body, the rear end of the air passage is connected with an air charging device through an air pipe, and an exhaust valve is arranged on the air pipe.
Further, the inner diameter of the catheter is + -1.8 mm.
Further, the inner diameter of the branch pipeline is +/-0.6 mm, and the length of the branch pipeline is 1-5cm.
Further, the lengths of the main pipeline at two sides of the branch pipeline are respectively 0.5cm and 1cm; the included angle between the short end of the main pipeline and the branch pipeline is 30-50 degrees.
The beneficial effects of the utility model are as follows: the rat middle cerebral artery thrombus device has the advantages of simple structure, convenient operation, no need of cutting off external carotid artery and branches thereof in the use process, small operation wound, low requirement on the experience skills of operator ligation and operation, time saving and improvement of the success rate of the model.
Drawings
The utility model is further described below with reference to the drawings and examples.
Fig. 1 is a schematic structural diagram of embodiment 1.
Fig. 2 is a schematic structural diagram of embodiment 2.
Fig. 3 is a schematic diagram of a structure of a wire bolt.
Detailed Description
The technical scheme of the present utility model will be clearly and completely described in the following detailed description.
Examples
As shown in fig. 1, the middle rat cerebral artery bolt device of the present embodiment comprises a catheter 1 and a bolt 2; the conduit is of a Y-shaped structure and comprises a main pipeline 11 and a branch pipeline 12 communicated with the main pipeline 11; the spool 2 passes from the branch conduit 12 into the main conduit 11. The inner diameter of the catheter 1 is + -1.8 mm. The length of the branch conduit 12 is 1-5cm. The length of the main pipeline 11 at two sides of the branch pipeline 12 is 0.5cm and 1cm respectively; the short end of the main pipe 11 forms an included angle of 30-50 degrees with the branch pipe 12. The plug 2 of the present utility model includes a main body 21, a head end blocking portion 22, and a tail end blocking portion 23.
Preferably, the catheter 1 of the present utility model is configured at both ends in a tapered configuration to facilitate placement into the external carotid artery.
In this embodiment, the head end plugging portion 22 and the tail end plugging portion 23 are solid silica gel heads, the silica gel heads are of columnar structures, the diameter of the head end plugging portion 22 is about 0.35mm, and a narrow portion is arranged in the middle of the branch pipeline 12 and is matched with the plugging heads.
The method for adopting the rat middle cerebral artery thrombus device comprises the following steps:
(1) After the rat is anesthetized, the rat is fixed in a supine position, and is placed on an operating table, and is routinely prepared for skin, sterilized and subjected to thermal insulation in the whole operation process. Hooking and fixing the rat incisors by using a suture line, so that the neck is stretched, and the subsequent operation is convenient;
(2) Using a conventional ophthalmic scissors or scalpel to make a incision from the middle of the neck, blunt separating the glandular tissue of the neck and fascia using forceps, exposing and separating the Common Carotid Artery (CCA), external Carotid Artery (ECA) and Internal Carotid Artery (ICA);
(3) ICA and its extracranial branch pterygoid-jaw arteries were isolated, the flap was tied with suture, and the pterygoid-jaw arteries were ligated to avoid insertion of a suture plug into the pterygoid-jaw artery, keeping the only open branch of CCA at ICA.
(4) Clamping CCA and ECA at the proximal end by using an arterial clamp, cutting a small opening on the upper part of the carotid artery, placing the catheter 1 into the carotid artery from the small opening, sealing the small opening by using tissue adhesive, and fixing the catheter at the bifurcation of the carotid artery by using a suture;
(5) The wire bolt enters the guide pipe from the branch pipe 12 of the guide pipe, and the silica gel heads at the head end and the tail end of the wire bolt respectively block one end of the main pipe 11 and the tail end of the branch pipe 12;
(6) Restoring blood supply of common carotid artery, external carotid artery and jawbone, sterilizing, sewing subcutaneous tissue and skin layer by layer, and placing into a cage after the animals are anesthetized and awakened. Obvious hemiplegia symptoms appear after the model making of the animals is revived, the body is inclined, and the crawling rotation is regarded as successful model making.
Examples
Referring to fig. 2 and 3, a middle rat cerebral artery bolt device of the present embodiment comprises a catheter 1 and a bolt 2; the conduit is of a Y-shaped structure and comprises a main pipeline 11 and a branch pipeline 12 communicated with the main pipeline 11; the spool 2 passes from the branch conduit 12 into the main conduit 11. The inner diameter of the catheter 1 is + -1.8 mm. The length of the branch conduit 12 is 1-5cm. The length of the main pipeline 11 at two sides of the branch pipeline 12 is 0.5cm and 1cm respectively; the short end of the main pipe 11 forms an included angle of 30-50 degrees with the branch pipe 12.
The plug 2 of the present utility model includes a main body 21, a head end blocking portion 22, and a tail end blocking portion 23. The head end plugging part 22 and the tail end plugging part 23 of the wire bolt of the embodiment are plugging air bags arranged on the peripheries of the head end and the tail end of the wire bolt main body respectively, an air passage 24 communicated with the plugging air bags is arranged in the main body, the rear end of the air passage is connected with an air charging device through an air pipe 25, and a two-way valve 26 is arranged on the air pipe.
The air flue rear end of this embodiment is provided with the syringe connecting seat, aerating device be the syringe, be provided with two-way valve control air flue on the trachea and open the air flue, aerify in the syringe net air flue, in the shutoff gasbag is gone into through the line bolt main part to gas.
The method for adopting the rat middle cerebral artery thrombus device comprises the following steps:
(1) After the rat is anesthetized, the rat is fixed in a supine position, and is placed on an operating table, and is routinely prepared for skin, sterilized and subjected to thermal insulation in the whole operation process. Hooking and fixing the rat incisors by using a suture line, so that the neck is stretched, and the subsequent operation is convenient;
(2) Using a conventional ophthalmic scissors or scalpel to make a incision from the middle of the neck, blunt separating the glandular tissue of the neck and fascia using forceps, exposing and separating the Common Carotid Artery (CCA), external Carotid Artery (ECA) and Internal Carotid Artery (ICA);
(3) ICA and its extracranial branch pterygoid-jaw arteries were isolated, the flap was tied with suture, and the pterygoid-jaw arteries were ligated to avoid insertion of a suture plug into the pterygoid-jaw artery, keeping the only open branch of CCA at ICA.
(4) Clamping CCA and ECA at the proximal end by using an arterial clamp, cutting a small opening on the upper part of the carotid artery, placing the catheter 1 into the carotid artery from the small opening, sealing the small opening by using tissue adhesive, and fixing the catheter at the bifurcation of the carotid artery by using a suture;
(5) The wire bolt enters the catheter from the branch pipeline 12 of the catheter, the injector inflates the plugging air bag, and the plugging air bag at the head end and the plugging air bag at the tail end of the wire bolt plug one end of the main pipeline 11 and the tail end of the branch pipeline 12 respectively;
(6) Restoring blood supply of common carotid artery, external carotid artery and jawbone, sterilizing, sewing subcutaneous tissue and skin layer by layer, and placing into a cage after the animals are anesthetized and awakened. Obvious hemiplegia symptoms appear after the model making of the animals is revived, the body is inclined, and the crawling rotation is regarded as successful model making.
The foregoing description of the preferred embodiments of the present utility model is not intended to limit the utility model, and those skilled in the art may make various modifications and equivalents within the spirit and scope of the utility model, and such modifications and equivalents should also be considered as falling within the scope of the technical solution of the present utility model.
Claims (8)
1. A rat middle cerebral artery thrombus device, characterized in that: comprises a catheter (1) and a wire bolt (2); the guide pipe is of a Y-shaped structure and comprises a main pipe (11) and a branch pipe (12) communicated with the main pipe (11); the wire bolt (2) enters the main pipeline (11) from the branch pipeline (12).
2. A rat middle cerebral artery bolt device as claimed in claim 1, wherein: the wire bolt (2) comprises a main body (21), a head end plugging part (22) and a tail end plugging part (23).
3. A rat middle cerebral artery bolt device according to claim 2, wherein: the head end plugging part and the tail end plugging part are solid silica gel bodies, and the solid silica gel bodies are cylindrical structures.
4. A rat middle cerebral artery bolt device according to claim 3, wherein: the diameters of the solid silica gel bodies of the head end plugging part and the tail end plugging part of the wire bolt are +/-0.35 mm, and the distance between the head end and the tail end is +/-20 mm.
5. A rat middle cerebral artery bolt device according to claim 2, wherein: the head end plugging part (22) and the tail end plugging part (23) can also be plugging air bags respectively arranged at the peripheries of the head end and the tail end of the wire bolt main body, an air passage communicated with the air bag plugging air bags is arranged in the main body, the rear end of the air passage is connected with an air charging device through an air pipe, and an exhaust valve is arranged on the air pipe.
6. A rat middle cerebral artery bolt device as claimed in claim 1, wherein: the inner diameter of the catheter (1) is +/-1.8 mm.
7. A rat middle cerebral artery bolt device as claimed in claim 1, wherein: the inner diameter of the branch pipeline (12) is +/-0.6 mm, the inner diameter of the middle part with smaller diameter is +/-0.34 mm, and the length is 1-5cm.
8. A rat middle cerebral artery bolt device as claimed in claim 1, wherein: the lengths of the main pipeline (11) at two sides of the branch pipeline (12) are respectively 0.5cm and 1cm; the included angle between the short end of the main pipeline (11) and the branch pipeline (12) is 30-50 degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322649846.5U CN220988954U (en) | 2023-09-28 | 2023-09-28 | Rat middle cerebral artery line thrombus device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322649846.5U CN220988954U (en) | 2023-09-28 | 2023-09-28 | Rat middle cerebral artery line thrombus device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220988954U true CN220988954U (en) | 2024-05-24 |
Family
ID=91115512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322649846.5U Active CN220988954U (en) | 2023-09-28 | 2023-09-28 | Rat middle cerebral artery line thrombus device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220988954U (en) |
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2023
- 2023-09-28 CN CN202322649846.5U patent/CN220988954U/en active Active
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