CN211863021U

CN211863021U - Expandable wire bolt for manufacturing animal ischemic stroke model

Info

Publication number: CN211863021U
Application number: CN201920830491.0U
Authority: CN
Inventors: 王祎媛; 方莹莹; 杜展莹; 陈莉婧; 汪铁山
Original assignee: Pingdingshan Yushun Biotechnology Co ltd
Current assignee: Pingdingshan Yushun Biotechnology Co ltd
Priority date: 2019-06-03
Filing date: 2019-06-03
Publication date: 2020-11-06
Anticipated expiration: 2029-06-03

Abstract

The utility model discloses an expandable wire bolt for manufacturing animal ischemic stroke models. The device comprises a nylon wire, wherein an expandable silica gel sleeve is coated on the outer side of the front end of the nylon wire, and the peripheral diameter of the silica gel sleeve is slightly smaller than or equal to the inner diameter of a blood vessel of an intracerebral artery to be blocked by an experimental animal. Silica gel is coated on the head section of the nylon thread, so that the silica gel and the nylon thread are integrated, when the cerebral artery is blocked, the expandable silica gel can be rapidly expanded when meeting water, and after the blood vessel is completely blocked, the expansion stops due to the fact that the expandable silica gel cannot contact with blood, and the blood vessel is completely blocked. Compare with current line bolt, this line bolt can effectual solution: the model of the wire-bolt can not be correctly selected because the skull base blood vessel does not accord with the real weight due to various reasons; the large and small mice can not be modeled after being too heavy; the skull base blood vessel of the large animal is complex and can not be used for making a model.

Description

Expandable wire bolt for manufacturing animal ischemic stroke model

The technical field is as follows:

the utility model belongs to the field of medical equipment, concretely relates to can be used to big animal such as big, mouse and rabbit, dog, pig, monkey and have an expandable wire bolt of cerebral arterial thrombosis.

Background art:

the MCAO model made in the prior art is generally (in the case of mice): weighing body weight, selecting a model of a thread plug corresponding to the body weight, after anesthetizing a mouse, carrying out conventional skin preparation and disinfection, longitudinally incising skin in a neck, separating an external carotid artery bluntly, tying and tensioning the external carotid artery at the far-end by using a 6-0 surgical thread, tying a near-heart end by using a 4-0 surgical thread, tying a broken knot in the middle of two threads by using 6-0, cutting an incision by using a Venus scissors, inserting the thread plug into the external carotid artery, tightening the 6-0 broken knot, fixing the thread plug, cutting a dead knot at the near-heart end, cutting the external carotid artery above the incision of the thread plug, lifting the residual end of the external carotid artery by using a microscope by a left hand to turn around, inserting the thread plug into the internal carotid artery, entering a willis ring through the internal carotid artery, stopping the thread inlet after resistance is sensed, and blocking a Middle Cerebral Artery (MCA) inlet by using a head section of silica gel at the moment to cause a focal cerebral ischemia model of local forceps. The plug may be removed for reperfusion after a certain period of ischemia, as required by the experiment.

However, the existing wire bolt has many defects: 1. in the current scientific research, a transgenic and gene knockout large mouse is used, and the mouse has a difference between a blood vessel and a wild mouse due to the change of genes, so that the model failure can be caused by the fact that the head end of the silica gel of the line bolt can not block the MCA by simply selecting the model of the line bolt according to the weight; 2. for wild rats and mice, the weight of the mice cannot grow normally according to a growth curve due to unqualified feeding environment, although the weights of the mice are the same, the diameters of cranial base blood vessels are different, and under the condition, the blood vessels cannot be completely blocked by simply selecting the model of the wire plug according to the weight, so that the model fails; 3. in the current experimental design, a lot of drugs are administered (such as continuously administering drugs for one month) or surgical treatment (such as brain stereotaxic treatment) before the model is made, the treatments can influence the growth of the mice, so that the mice can not normally gain weight according to a growth curve, and at the moment, if the model is selected according to the weight, the wire bolt can not block MCA, so that the model fails; 4. for rats with extremely large weight, the diameter of the head section of the wire plug is correspondingly large, the willis ring of the internal carotid artery turning into the skull base has a bend, and the wire plug with the large diameter cannot turn the bend, so that the rats with large weight cannot make a focal cerebral ischemia model; 5. for large animals such as rabbits, dogs, pigs, monkeys, etc., because their skull bases willis thick and the middle cerebral arteries have many bifurcations, unlike experimental mice that are closed groups or inbred lines, the large animals have the same body weight and the individual differences are still large, and thus a suitable plug cannot be selected for molding.

Therefore, the existing silica gel wire plug can not meet the requirements of transgenic and gene knockout mice with the diameter of blood vessels changed due to gene change in the current scientific research, big and small mice which can not grow according to a normal growth curve due to various intervention reasons, and various big animals for manufacturing stable stroke models.

The invention content is as follows:

the utility model aims at providing a can get into skull base willis ring, thereby the line bolt that can expand rapidly comes to stop up MCA completely and goes into the stable apoplexy model of preparation of blood mouth and is used for making big, mouse and rabbit, and the line bolt of big animal ischemic cerebral apoplexy models such as dog, pig, monkey.

The utility model discloses a carry out through following technical scheme:

the utility model discloses a thread bolt that is used for making big, mouse and rabbit, big animal such as dog, pig, monkey ischemia cerebral apoplexy model, including the nylon wire, the front end outside coating of nylon wire has expandable silica gel cover, the peripheral diameter of silica gel cover slightly is less than and equals the internal diameter of blood vessel that experimental animals will block the cerebral artery.

Preferably, when used for rats, the nylon thread has a diameter of 0.254mm and a length of 50mm, the expandable silicone sleeve has a thickness of 0.1mm, and the expandable silicone sleeve has a length of 6 mm.

Preferably, when the rubber sleeve is used for a mouse, the nylon wire has the diameter of 0.104mm and the length of 30mm, the thickness of the expandable silica gel sleeve is 0.05mm, and the length of the expandable silica gel sleeve is 3 mm.

The utility model has the advantages that:

the utility model is used for make big, mouse and rabbit, the line bolt of big animal ischemic cerebral apoplexy models such as dog, pig, monkey, it wraps the head segment at the nylon wire with silica gel for silica gel becomes an organic whole with the nylon wire, when blockking the internal artery of brain, inflation silica gel meets water can expand rapidly, after blocking the blood vessel completely, because the contact can not blood and the inflation stops, thereby blocks up the blood vessel completely. Compare with current line bolt, this line bolt can effectual solution: the model of the wire-bolt can not be correctly selected because the skull base blood vessel does not accord with the real weight due to various reasons; the large and small mice can not be modeled after being too heavy; the skull base blood vessel of the large animal is complex and can not be used for making a model.

Description of the drawings:

fig. 1 is a schematic structural diagram of a wire plug of the present invention for manufacturing ischemic stroke models of large animals such as rats, mice, rabbits, dogs, pigs, monkeys, etc.;

wherein 1, nylon thread; 2. an expandable silica gel sleeve.

The specific implementation mode is as follows:

the following examples are further illustrative of the present invention and are not intended to be limiting thereof.

Example 1:

as shown in fig. 1, the thread plug for manufacturing models of cerebral ischemic stroke of large animals such as large and small mice, rabbits, dogs, pigs, monkeys, etc. of the present embodiment includes a nylon thread 1, an expandable silica gel sleeve 2 is coated outside the front end of the nylon thread, and the peripheral diameter of the silica gel sleeve is slightly smaller than or equal to the inner diameter of a blood vessel of an internal cerebral artery to be blocked by an experimental animal. The diameter of the nylon wire is 0.254mm, the length of the nylon wire is 50mm, the thickness of the expandable silica gel sleeve is 0.1mm, and the length of the expandable silica gel sleeve is 6 mm.

Example 2:

the embodiment is basically the same as the embodiment 1, except that the nylon wire has a diameter of 0.104mm and a length of 30mm, the thickness of the expandable silica gel sleeve is 0.05mm, and the length of the expandable silica gel sleeve is 3 mm.

When the wire plug used for manufacturing the cerebral arterial thrombosis model of large animals such as big and small mice, rabbits, dogs, pigs, monkeys and the like is used for manufacturing the MCAO model, the operation steps are as follows (taking the small mice as an example):

1. after the mouse is anesthetized, the skin is prepared and disinfected conventionally, the skin is longitudinally incised in the neck, and the external carotid artery 5 is separated bluntly;

2. tying and tightening the distal end of the external carotid artery with 6-0 surgical thread, tying the proximal end with 4-0 surgical thread, and tying a virtual knot between two threads with 6-0;

3. cutting an incision with Venus scissors, inserting the thread plug of the embodiment into the external carotid artery from the incision, tensioning a 6-0 virtual knot, fixing the thread plug, and then cutting off a dead knot at the proximal end;

4. cutting off the external carotid artery above the incision of the plug, lifting the residual end of the external carotid artery with a pair of micro-forceps by the left hand to turn around, then inserting the plug into the internal carotid artery, entering a willis ring through the internal carotid artery, stopping the wire inlet after resistance is felt, and blocking the blood flow in the middle cerebral artery;

5. after a period of ischemia, the plug was pulled out and the skin was sutured.

6. The wire plugs of this example were inserted into the willis's annulus to block blood flow in the middle cerebral artery, and in the above-described MCAO model, only the wire plugs were inserted into the blood vessels to a predetermined depth, and the model was made using the wire plugs, which is comparable to the wire plugs currently used on the market: a. the change of the inner diameter of the blood vessel caused by the gene change of the experimental animal is avoided; b. the influence of the non-correspondence of the body weight and the inner diameter of the blood vessel caused by preoperative experimental operation or the fact that the body weight does not grow according to the normal growth curve rule due to the change of the feeding environment is avoided; c. is not affected by large individual difference of large animals. Therefore, experimental animals with various special conditions can be used for manufacturing MCAO models, the damage to various animals is small, and the success rate of molding is improved.

7. In conclusion, the novel wire plug of the experiment and the standardized operation steps can completely block MCA blood flow, so that a stable focal ischemia model is created.

8. The technical principle of the present invention is described above with reference to specific embodiments. The description is made for the purpose of illustrating the principles of the invention and should not be construed in any way as limiting the scope of the invention. Other embodiments of the present invention will occur to those skilled in the art based on the explanations herein without any creative effort.

All of these ways will fall within the scope of protection of the present invention.

Claims

1. An expandable wire plug for manufacturing an animal model with cerebral arterial thrombosis is characterized by comprising a nylon wire, wherein the outer side of the front end of the nylon wire is coated with an expandable silica gel sleeve, and the peripheral diameter of the silica gel sleeve is slightly smaller than or equal to the inner diameter of a blood vessel of an internal cerebral artery to be blocked by an experimental animal;

the diameter of the nylon wire is 0.254mm, the length of the nylon wire is 50mm, the thickness of the expandable silica gel sleeve is 0.1mm, and the length of the expandable silica gel sleeve is 6 mm; or the diameter of the nylon wire is 0.104mm, the length of the nylon wire is 30mm, the thickness of the expandable silica gel sleeve is 0.05mm, and the length of the expandable silica gel sleeve is 3 mm.