CN211325826U - Model making assembly for experimental animal with focal permanent cerebral ischemia - Google Patents

Abstract

The utility model relates to a focus permanent cerebral ischemia experimental animals makes mould subassembly, including operation fixed plate, four limbs fixed column, expander fixed column, neck bed hedgehopping pillow, operation window expander, separation threading apparatus, carotid artery both ends blocker, puncture ware, bolt line. Its advantages are: the precise degeneration standardizes the molding experimental tool, and ensures that the experimental operation has more normativity and repeatability; in the experiment, the neck of the rat is placed on the neck heightening column, so that the exposure of the carotid artery of the rat is facilitated; the operation window expander can replace elbow fine forceps and has a fixing effect; the operation can be simplified by separating the threading device, so that the separation, the threading and the threading are carried out simultaneously; the puncture outfit and the thread form a sleeve structure, the needle and the thread are integrated, the wound is small, the extravasated blood is less, and the adventitia and the nerve are not easy to be cut by mistake; the blood vessel ligation operation is not required to be carried out by using more operation lines, so that the operation visual field is not interfered; the head of the plug thread is sphere-like, which is beneficial to the plug thread to pass through the blood vessel and prevents the blood vessel wall from being damaged.

Description

Model making assembly for experimental animal with focal permanent cerebral ischemia

Technical Field

The utility model belongs to the technical field of experimental apparatus technique and specifically relates to a local permanent cerebral ischemia experimental animals makes mould subassembly.

Background

Stroke is an acute cerebrovascular disease, a group of diseases in which brain tissue is damaged due to sudden rupture of cerebral vessels or failure of blood flow into the brain due to vascular occlusion, and includes ischemic and hemorrhagic stroke. The ischemic stroke accounts for 60 to 70 percent of the total stroke, and has the characteristics of high morbidity, high mortality and high disability rate. Therefore, the research on the etiology and pathogenesis of ischemic stroke and the exploration on the treatment method of stroke have great significance, and the preparation of related animal models is an important way for researching stroke.

The Middle Cerebral Artery Occlusion (MCAO) model is a classic animal model for researching ischemic cerebral apoplexy, and Koizumi et al establish a rat model for preparing focal cerebral ischemia by a thread-plug method. The model can ensure the constant ischemic position, can carry out reperfusion, simulates different states of permanent and transient focal cerebral ischemia of human beings, does not need craniotomy, can accurately control ischemia and recanalization time, has relatively small operation difficulty and low rat mortality and postoperative complications. The principle is that a nylon material wire is inserted into the middle cerebral artery of an animal through an internal carotid artery to block blood flow to cause local cerebral ischemia.

The general model preparation method is as follows: 1) the animal is anesthetized and fixed on a mouse board in a supine position, the animal is disinfected conventionally after shearing, and an opening of 2-3 cm is made along the median line of the neck by using an operating scissors. 2) The fascia and muscle tissues were separated blunt along the median with elbow fine forceps, the bronchi were exposed, and the carotid artery was looked down along one side of the sternocleidomastoid tendon. 3) The arterial sheath was carefully separated with an elbow hemostat, during which time the vagus nerve, which accompanies the carotid artery, could not be injured, otherwise the experimental animals died immediately. The common, external and internal carotid arteries were then isolated. 4) The common carotid artery and external carotid artery were ligated with nylon thread. 5) The common carotid artery is clamped by an artery clamp, a small opening is cut at the common carotid artery by an ophthalmic scissors, a plug wire is inserted towards the direction of the neck, the fixing wire is fastened, and the artery clamp is loosened. 6) The wound is sutured in sequence and then disinfected conventionally, and experimental animals should take warm-keeping measures after the operation to avoid death caused by hypothermia. Reperfusion was performed after 2 hours. And (4) after the rat is awake, performing behavioral scoring, and if the rat is unstable in standing and paralyzed in the left limb, and turns to one side when lifting the tail, indicating that the MCAO model is successfully established.

1. There is currently no standardized, dedicated modeling component for the MCAO model. The prior experimental tools in the molding process comprise surgical scissors, fine forceps, elbow hemostatic forceps, fine curved forceps, artery clamps, thick wires, thin wires, thread plugs and suture needles. The tools are various and the specificity is poor.

2. In the traditional molding process, the animal is anesthetized and fixed on a mouse board in a supine position, and the neck median line is opened by using an operating scissors. Because the artery is deep, the artery is not easy to expose, the operation visual field is narrow, and the operation is not easy.

3. The thin tweezers of elbow, the blunt separation submaxillary gland of surgical scissors and blood vessel surrounding tissue are used to tradition molding in-process, and thin tweezers of elbow, scissors are difficult fixed, if touch thin tweezers, surgical scissors in the experimentation by mistake and can cause the blood vessel to break, influence the operation field of vision, disturb experimental operation, arouse the experiment failure even, have improved scientific research cost.

4. In the traditional molding process, an elbow hemostatic forceps is used for separating an arterial sheath, and a nylon thread is used for ligating a common carotid artery and an external carotid artery under the cooperation of a thin curved forceps. The vagus nerve of the rat is easily injured by an arterial sheath separated by using an elbow hemostatic forceps, and if the vagus nerve is injured, the respiratory reflex of the rat is influenced, so that the rat is dead, and the modeling fails. In the experiment, the bent forceps are easy to puncture blood vessels, once the carotid artery is damaged, the blood is often difficult to stop, and a rat shocks and even dies after a large amount of blood loss, so that the molding failure is caused. Meanwhile, the experimental operation is closely related to the operation level and proficiency of the experimenter.

5. In the traditional molding process, an artery clamp is used for clamping and closing an internal carotid artery, a small opening is cut at the common carotid artery by an ophthalmic scissors, and the size of the opening directly influences the difficulty of wire tying and inserting. The angle and the size of the incision are difficult to master, and the ophthalmic scissors are easy to cut adventitia and blood vessel by mistake. The experimental operation is closely related to the level of operation and proficiency of the experimenter.

6. In the existing experimental method, the common carotid artery, the external carotid artery, the internal carotid artery and the wound of the ligature need to be ligatured in sequence, and after the ligature is ligatured by using the operation ligature for many times, more residual ligatures are left, so that the operation visual field is easily interfered.

7. The existing embolism wire is mostly self-made, and has certain deletion in the aspect of stability, the blunt round wire head of the existing embolism wire is an important part for avoiding the damage of a blood vessel wall in the process of inserting the embolism wire into the blood vessel in the prior art, and the key for successfully molding the thrombus wire is found in our experiment by the inherent diameter of the embolism wire and the weight matching degree of a mouse. The thrombus-making thread runs to the internal carotid artery, so that the blood vessel wall is easily injured, and skull base bleeding and arachnoid bleeding are caused, and the molding failure is caused.

In view of the above, there is an urgent need for a modeling component for experimental animals with focal permanent cerebral ischemia.

Chinese patent documents: CN105326578A, published: 2016.02.17, discloses a method for making animal model of cerebral ischemia reperfusion injury, using sodium pentobarbital to anaesthetize the neck of the animal, cutting the skin on two sides of the neck, separating the common carotid artery and vagus nerve on two sides, cutting the common carotid artery on two sides or implanting a saccule catheter in the common carotid artery on two sides, and inflating the balloon catheter in the implanted saccule catheter until the saccule catheter blocks the initial part of the common carotid artery on two sides in the brain, the ischemia lasts for 2.5-3h, and the animal with cerebral ischemia injury is made; cutting off common carotid arteries at both sides for 2h, connecting the common carotid arteries at both sides with a hose, suturing, and injecting blood into the hose; or the gas in the balloon catheter is discharged, so that the blood is perfused again in the ischemic area; neurological deficit scoring was performed at different times as required and recorded with a video recorder.

Chinese patent documents: CN104983480A, published: 2015.10.21, discloses a method for establishing a cerebral ischemia reperfusion model of rat external carotid artery indwelling vessel, which comprises the following steps: dissociating the direction of the external carotid artery blood vessel of the rat, and dissociating the direction of the external carotid artery blood vessel of the rat to a position which is consistent with or parallel to the direction of the internal carotid artery blood vessel of the rat; an incision is made in the external carotid artery blood vessel to insert a wire plug, and cerebral ischemia is formed in the neck of a rat; after the insertion of the wire plug to form cerebral ischemia for 1-2 hours, the wire plug is pulled out and then placed into a catheter injection sac capable of repeatedly injecting medicine.

However, the model building component for the experimental animal with the focal permanent cerebral ischemia has not been reported at present.

Disclosure of Invention

The utility model aims at providing a local permanent cerebral ischemia experimental animals makes module.

In order to achieve the purpose, the invention adopts the technical scheme that:

a model building assembly for a focal permanent cerebral ischemia experimental animal comprises a surgery fixing plate 1, limb fixing columns 2, dilator fixing columns 3, a neck heightening pillow 4, a surgery window dilator 5, a separation threading device 6, a carotid artery two-end blocker 7, a puncture device 8 and a embolism line 9;

the operation fixing plate 1 is in a cuboid plate shape, an incisor fixing device 11 is hinged to the upper surface of the operation fixing plate 1, and four bases 12 are arranged below the operation fixing plate 1; the base 12 is provided with an anti-skid layer 13;

the front of the operation fixing plate 1 is adsorbed with a four limbs fixing column 2, an expander fixing column 3 and a neck heightening pillow 4; the four-limb fixing column 2 consists of a bottom column 21 and clamping strips 22, and the two clamping strips 22 are fixedly connected to the upper surface of the bottom column 21; the middle part of the side surface of the expander fixing column 3 is provided with a connecting groove 31;

the operation window expander 5 consists of a fixed hook 51, a thin elastic rope 52, a thick elastic rope 53 and a fixed ring 54; two ends of the thick elastic rope 53 are connected with a thin elastic rope 52; one of the two thin elastic ropes 52 is connected with a fixed hook 51, and the other one is connected with a fixed ring 54; the fixing ring 54 is sleeved in the connecting groove 31 of the expander fixing column 3;

the separation threading device 6 consists of a threading separation head 61, an operating rod 62 and a handle 63; one end of the operating rod 62 is fixedly connected with a threading separation head 61, and the other end is fixedly connected with a handle 63;

the carotid artery two-end blocker 7 consists of a blocking plate 71, a left arc-shaped connecting rod 72, a right arc-shaped connecting rod 73, a handle 74 and a spring 75; two ends of the left arc-shaped connecting rod 72 are fixedly connected with blocking plates 71; the middle part of the left arc-shaped connecting rod 72 is fixedly connected with a handle 74 and a shaft hole 77; both ends of the right arc-shaped connecting rod 73 are fixedly connected with blocking plates 71, and the blocking plates 71 are provided with soft pads 76; the middle part of the right arc-shaped connecting rod 73 is fixedly connected with a handle 74 and a shaft pin 78; the shaft pin 78 is arranged in the shaft hole 77 to hinge the left arc-shaped connecting rod 72 and the right arc-shaped connecting rod 73, the spring 75 is arranged in the shaft hole 77, one end of the spring 75 is connected with the shaft hole 77, and the other end is connected with the shaft pin 78;

the puncture outfit 8 comprises a puncture needle point 81, a needle stem 82 and a needle holding handle 83; one end of the needle stem 82 is provided with a puncture needle point 81, and the other end is fixedly connected with a needle holding handle 83; a plug wire inlet 84 is arranged on the puncture needle tip 81, and a plug wire outlet 85 is arranged on the needle holding handle 83;

the thread 9 is inserted into the puncture outfit 8 through the needle point part of the puncture outfit 8, the head part of the thread 9 is provided with a sphere-like thread head 91, and the surface of the thread is provided with scales 92.

The four limbs fixing column 2, the expander fixing column 3 and the neck heightening pillow 4 are all provided with strong magnets at the bottoms and are used for being adsorbed in front of the operation fixing plate 1.

The shaft pin 78 is matched with the shaft hole 77; the bolt line 9 is transparent, and the scale 92 is marked on the surface of the bolt line 9.

The utility model has the advantages that:

1. there is currently no standardized, dedicated modeling component for the MCAO model. The utility model discloses be used for the line bolt method to prepare the rat model of focal cerebral ischemia specially. The molding experiment tool is simplified, and the experimental operation is guaranteed to have more normativity and repeatability.

2. In the traditional molding process, the animal is anesthetized and fixed on a mouse board in a supine position, and an opening is formed along the median line of the neck by using an operating scissors. Because the artery is deep, the artery is not easy to expose, the experimental visual field is narrow, and the operation is not easy. The utility model discloses a set up neck bed hedgehopping post on the operation fixed plate among the modeling subassembly, arrange the neck bed hedgehopping post in with the mouse neck in the experiment, do benefit to the carotid artery that exposes the mouse and show loudly, enlarge the operation field simultaneously, the experimental operation of being convenient for.

3. The blunt separation of elbow fine tweezers, scissors is used to tradition molding in-process, and elbow fine tweezers, scissors are not fixed well, and touch fine tweezers, scissors occasionally can influence the operation field of vision among the experimentation, interference experiment operation. The operation window expander in the molding assembly of the utility model can replace the elbow fine forceps and can be fixed on the operation fixing plate. Can play a firm fixing role and enlarge the operation visual field.

4. In the traditional molding process, an elbow hemostatic forceps is used for separating an arterial sheath, and then a nylon wire is used for ligating a common carotid artery and an external carotid artery under the cooperation of a thin curved forceps. The vagus nerve of the rat is easily injured by an arterial sheath separated by using an elbow hemostatic forceps, and if the vagus nerve is injured, the respiration of the rat can be influenced, so that the rat dies, and the modeling fails. In the experiment, the bent forceps are easy to damage blood vessels, and once the arteries are damaged, mice lose blood and die, so that the modeling fails. Meanwhile, the experimental operation is closely related to the operation level and proficiency of the experimenter. In the above experimental process, the arterial sheath needs to be separated and then ligated. The utility model discloses a separation threading apparatus among the molding assembly can simplify above-mentioned operation, reaches separation, threading, lead wire and goes on simultaneously. The threader compares in curved tweezers to the damage of blood vessel littleer, can protect the vascular wall, the smooth of guarantee molding goes on.

5. The internal carotid artery is clamped and closed by an artery clamp in the traditional molding process, a small opening is cut at the position of the common carotid artery by an ophthalmic scissors, and the size of the opening directly influences whether a embolus line can be smoothly inserted into the common carotid artery. The angle and the size of the incision are difficult to master, and the ophthalmic scissors are easy to cut adventitia and blood vessels by mistake. The experimental operation is closely related to the level of operation and proficiency of the experimenter. The utility model discloses a puncture ware among the molding assembly forms "sleeve pipe" structure with the bolt line, and the needle line integration, the wound is little, and is few to external oozing blood, and is difficult for the mistake to cut the adventitia, and easy operation.

6. In the traditional experiment, the operation thread needs to be used for ligation for many times, the thread ends are more, and the operation process is easy to interfere. The utility model discloses a through using separation ware and puncture ware in the molding assembly, need not use more operation line, can not disturb the operation field of vision.

7. According to the traditional modeling method, a plug wire is inserted into a common carotid artery, and then the plug wire passes through the common carotid artery to an internal carotid artery, so that the blood vessel wall is easily damaged in the process, skull base bleeding and arachnoid bleeding are caused, and the modeling failure is caused. The utility model discloses a bolt line in the molding assembly is tied the line head by the quasi-spherical and is tied the line with the scale and constitute. The head of the plug thread is spherical, which is beneficial to the plug thread to pass through the blood vessel and prevents the blood vessel wall from being damaged.

Drawings

FIG. 1 is a schematic structural diagram of a modeling component of an experimental animal with focal permanent cerebral ischemia.

FIG. 2 is a schematic structural diagram of a surgical fixing plate of a model building component of an experimental animal with focal permanent cerebral ischemia.

FIG. 3 is a schematic diagram of a second surgical fixing plate of a molding assembly of an experimental animal with focal permanent cerebral ischemia.

FIG. 4 is a schematic diagram of a limb fixing column structure of a modeling component of an experimental animal with focal permanent cerebral ischemia.

Fig. 5 is a structural schematic diagram of a dilator fixing column of a local permanent cerebral ischemia experimental animal modeling assembly.

FIG. 6 is a schematic view of a neck pillow of a modeling component of a focal permanent cerebral ischemia experimental animal.

FIG. 7 is a schematic structural view of an operation window dilator of a model assembly of an experimental animal with focal permanent cerebral ischemia.

FIG. 8 is a schematic structural view of a separation threading apparatus of a modeling component of an experimental animal with focal permanent cerebral ischemia.

FIG. 9 is a schematic structural diagram of a carotid artery two-end blocker of a modeling component of a focal permanent cerebral ischemia experimental animal.

FIG. 10 is a schematic structural diagram II of a carotid artery two-end blocker of a modeling component of a focal permanent cerebral ischemia experimental animal.

FIG. 11 is a schematic structural view of a puncture outfit of a model-making component of an experimental animal with focal permanent cerebral ischemia.

FIG. 12 is a schematic diagram of a plug wire structure of a modeling component for a focal permanent cerebral ischemia experimental animal.

Detailed Description

The invention will be further described with reference to the following examples and with reference to the accompanying drawings.

The reference numerals and components referred to in the drawings are as follows:

1. operation fixing plate

11. Portal tooth fixing device

12. Base seat

13. Anti-skid layer

2. Four limbs fixing column

21. Bottom pillar

22. Holding strip

3. Dilator fixing column

31. Connecting groove

4. Neck pillow

5. Operation window expander

51. Fixing hook

52. Thin elastic rope

53. Thick elastic rope

54. Fixing ring

6. Separating threading device

61. Threading separation head

62. Operating rod

63. Handle (CN)

7. Carotid artery two-end blocking device

71. Blocking plate

72. Left side arc connecting rod

73. Right arc connecting rod

74. Handle bar

75. Spring

76. Soft cushion

77. Shaft hole

78. Axle pin

8. Puncture outfit

81. Puncture needle tip

82. Needle stalk

83. Needle holding handle

84. Bolt line inlet

85. Bolt outlet

9. Bolt line

91. Spherical bolt wire end

92. Scale division

Example 1

Referring to fig. 1, fig. 1 is a schematic structural diagram of a modeling assembly for a focal permanent cerebral ischemia experimental animal according to this embodiment. The model building assembly for the experimental animal with the focal permanent cerebral ischemia comprises a surgery fixing plate 1, four limb fixing columns 2, dilator fixing columns 3, a neck heightening pillow 4, a surgery window dilator 5, a separation threading device 6, a carotid artery two-end blocker 7, a puncture device 8 and a embolism line 9;

referring to fig. 2 and 3, fig. 2 is a first schematic structural diagram of a surgical fixing plate of a modeling component of a focal permanent cerebral ischemia experimental animal of the present embodiment, and fig. 3 is a second schematic structural diagram of a surgical fixing plate of a modeling component of a focal permanent cerebral ischemia experimental animal of the present embodiment. The operation fixing plate 1 is in a cuboid plate shape, an incisor fixing device 11 is hinged to the upper surface of the operation fixing plate 1, and four bases 12 are arranged below the operation fixing plate 1; the base 12 is provided with an anti-skid layer 13;

referring to fig. 4-6, fig. 4 is a schematic diagram of a limb fixing post structure of a modeling assembly of a focal permanent cerebral ischemia experimental animal of the present embodiment, fig. 5 is a schematic diagram of a dilator fixing post structure of a modeling assembly of a focal permanent cerebral ischemia experimental animal of the present embodiment, and fig. 6 is a schematic diagram of a neck bolster structure of a modeling assembly of a focal permanent cerebral ischemia experimental animal of the present embodiment. The front of the operation fixing plate 1 is adsorbed with a four limbs fixing column 2, an expander fixing column 3 and a neck heightening pillow 4; the four-limb fixing column 2 consists of a bottom column 21 and clamping strips 22, and the two clamping strips 22 are fixedly connected to the upper surface of the bottom column 21; the middle part of the side surface of the expander fixing column 3 is provided with a connecting groove 31;

referring to fig. 7, fig. 7 is a schematic structural view of an operating window dilator of a modeling assembly of a focal permanent cerebral ischemia experimental animal according to the present embodiment. The operation window expander 5 consists of a fixed hook 51, a thin elastic rope 52, a thick elastic rope 53 and a fixed ring 54; two ends of the thick elastic rope 53 are connected with a thin elastic rope 52; one of the two thin elastic ropes 52 is connected with a fixed hook 51, and the other one is connected with a fixed ring 54; the fixing ring 54 is sleeved in the connecting groove 31 of the expander fixing column 3;

referring to fig. 8, fig. 8 is a schematic structural view of a separation threading apparatus of a modeling assembly of a focal permanent cerebral ischemia experimental animal according to the present embodiment. The separation threading device 6 consists of a threading separation head 61, an operating rod 62 and a handle 63; one end of the operating rod 62 is fixedly connected with a threading separation head 61, and the other end is fixedly connected with a handle 63;

referring to fig. 9 and 10, fig. 9 is a first schematic structural diagram of a first blocking device for two carotid ends of a modeling assembly of a focal permanent cerebral ischemia experimental animal of this embodiment, and fig. 10 is a second schematic structural diagram of a second blocking device for two carotid ends of a modeling assembly of a focal permanent cerebral ischemia experimental animal of this embodiment. The carotid artery two-end blocker 7 consists of a blocking plate 71, a left arc-shaped connecting rod 72, a right arc-shaped connecting rod 73, a handle 74 and a spring 75; both ends of the left arc-shaped connecting rod 72 are fixedly connected with blocking plates 71, and soft pads 76 are arranged on the blocking plates 71; the middle part of the left arc-shaped connecting rod 72 is fixedly connected with a handle 74 and a shaft hole 77; both ends of the right arc-shaped connecting rod 73 are fixedly connected with blocking plates 71, and the blocking plates 71 are provided with soft pads 76; the middle part of the right arc-shaped connecting rod 73 is fixedly connected with a handle 74 and a shaft pin 78; the shaft pin 78 is arranged in the shaft hole 77 to hinge the left arc-shaped connecting rod 72 and the right arc-shaped connecting rod 73, the spring 75 is arranged in the shaft hole 77, one end of the spring 75 is connected with the shaft hole 77, and the other end is connected with the shaft pin 78;

referring to fig. 11, fig. 11 is a schematic structural view of a puncture outfit of a modeling assembly for a focal permanent cerebral ischemia experimental animal according to this embodiment. The puncture outfit 8 comprises a puncture needle point 81, a needle stem 82 and a needle holding handle 83; one end of the needle stem 82 is provided with a puncture needle point 81, and the other end is fixedly connected with a needle holding handle 83; a plug wire inlet 84 is arranged on the puncture needle tip 81, and a plug wire outlet 85 is arranged on the needle holding handle 83;

referring to fig. 12, fig. 12 is a schematic diagram of a plug wire structure of a modeling assembly for a focal permanent cerebral ischemia experimental animal according to the present embodiment. The bolt line 9 penetrates through the puncture outfit 8, the head of the bolt line 9 is provided with a spherical bolt line head 91, and the inside of the bolt line 9 is provided with a scale 92.

The bottoms of the four-limb fixing column 2, the expander fixing column 3 and the neck heightening pillow 4 are all strong in magnetism and are used for being adsorbed in front of the operation fixing plate 1.

The shaft pin 78 is matched with the shaft hole 77.

The bolt line 9 is transparent, and the scale 92 is embedded in the bolt line 9.

Example 2

Referring to fig. 1, fig. 1 is a schematic structural diagram of a modeling assembly for a focal permanent cerebral ischemia experimental animal according to this embodiment.

The use process and the method of the animal modeling component for the focal permanent cerebral ischemia experiment comprise the following steps:

the animal is anesthetized and is fixed in front of the operation fixing plate 1 in the supine position, the neck is rested on the neck elevating pillow 4, the neck of the rat is simply sterilized, and the neck is opened at the median line of the neck by using the operation scissors.

The operative window dilator 5 is used for blunt separation along the center, the fixed hook 51 hooks the skin and the subjaw gland of the rat, and the fixed ring 54 is sleeved in the connecting groove 31 of the dilator fixed column 3. The carotid artery was looked down along the right sternocleidomastoid tendon.

The nylon thread passes through the threading separation head 61 on the separation threading device 6, the handle 63 on the separation threading device 6 is held by hand, the threading separation head 61 bypasses the artery, and then the threading separation head 61 is drawn back, so that the thread head automatically passes through the blood vessel. The common, external and internal carotid arteries were isolated.

The common carotid artery and external carotid artery were ligated with nylon thread.

The handle 74 of the two-end carotid occluder 7 is held in the hand and the common carotid artery is clamped by the occlusion plates 71, and the blood vessel in the middle of the two occlusion plates 71 can be used for puncture. The puncture instrument 8 is passed through the blood vessel between the two blocking plates 71 by passing the puncture wire 9 through the puncture wire inlet 84 of the puncture instrument and out of the puncture wire outlet 85 along the needle stem 82. At this time, the tether line 9 slides along the common carotid artery along the puncture instrument 8. When the carotid artery runs into the resistance, the puncture outfit 8 is pulled out, the blockers 7 at the two ends of the carotid artery are pulled out, and ligation is carried out.

The wound was sutured and reperfusion was performed after 2 hours. And (4) after the rat is awake, performing behavioral scoring, and if the rat is unstable in standing and paralyzed in the left limb, and turns to one side when lifting the tail, indicating that the MCAO model is successfully established.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and additions can be made without departing from the principles of the present invention, and these improvements and additions should also be regarded as the protection scope of the present invention.

Claims (3)

1. The model building assembly for the experimental animal with the focal permanent cerebral ischemia is characterized by comprising an operation fixing plate (1), four limb fixing columns (2), dilator fixing columns (3), a neck heightening pillow (4), an operation window dilator (5), a separation threading device (6), a carotid artery two-end blocker (7), a puncture device (8) and a embolism line (9);

the operation fixing plate (1) is in a cuboid plate shape, a front tooth fixing device (11) is hinged to the upper surface of the operation fixing plate (1), and four bases (12) are arranged below the operation fixing plate (1); the base (12) is provided with an anti-skid layer (13);

the front of the operation fixing plate (1) is adsorbed with a four-limb fixing column (2), an expander fixing column (3) and a neck heightening pillow (4); the four-limb fixing column (2) consists of a bottom column (21) and clamping strips (22), and the two clamping strips (22) are fixedly connected to the upper surface of the bottom column (21); the middle part of the side surface of the expander fixing column (3) is provided with a connecting groove (31);

the operation window expander (5) consists of a fixed hook (51), a thin elastic rope (52), a thick elastic rope (53) and a fixed ring (54); both ends of the thick elastic rope (53) are connected with a thin elastic rope (52); one of the two thin elastic ropes (52) is connected with a fixed hook (51), and the other one is connected with a fixed ring (54); the fixing ring (54) is sleeved in the connecting groove (31) of the expander fixing column (3);

the separation threading device (6) consists of a threading separation head (61), an operating rod (62) and a handle (63); one end of the operating rod (62) is fixedly connected with a threading separation head (61), and the other end of the operating rod is fixedly connected with a handle (63);

the carotid artery two-end blocker (7) consists of a blocking plate (71), a left arc-shaped connecting rod (72), a right arc-shaped connecting rod (73), a handle (74) and a spring (75); two ends of the left arc-shaped connecting rod (72) are fixedly connected with blocking plates (71); the middle part of the left arc-shaped connecting rod (72) is fixedly connected with a handle (74) and a shaft hole (77); both ends of the right arc-shaped connecting rod (73) are fixedly connected with blocking plates (71), and the blocking plates (71) are provided with soft pads (76); the middle part of the right arc-shaped connecting rod (73) is fixedly connected with a handle (74) and a shaft pin (78); the shaft pin (78) is arranged in the shaft hole (77) to hinge the left arc-shaped connecting rod (72) and the right arc-shaped connecting rod (73), the spring (75) is arranged in the shaft hole (77), one end of the spring (75) is connected with the shaft hole (77), and the other end of the spring (75) is connected with the shaft pin (78);

the puncture outfit (8) comprises a puncture needle point (81), a needle stem (82) and a needle holding handle (83); one end of the needle stem (82) is provided with a puncture needle point (81), and the other end is fixedly connected with a needle holding handle (83); a bolt line inlet (84) is arranged on the puncture needle point (81), and a bolt line outlet (85) is arranged on the needle holding handle (83);

the thread (9) is inserted into the puncture outfit (8) through the needle point part of the puncture outfit (8), the head part of the thread (9) is provided with a sphere-like thread head (91), and the surface of the thread is provided with scales (92).

2. The modeling assembly for experimental animals with focal permanent cerebral ischemia according to claim 1, wherein the bottoms of the limb fixing posts (2), the dilator fixing posts (3) and the neck heightening pillow (4) are all provided with strong magnets for being adsorbed in front of the operation fixing plate (1).

3. The modeling assembly for experimental animals with focal permanent cerebral ischemia according to claim 1, wherein the axle pin (78) is engaged with the axle hole (77); the bolt line (9) is transparent, and the scale (92) is printed on the surface of the bolt line (9).

Images