CN220977008U - High-flux honeycomb type automatic cell culture equipment - Google Patents

Abstract

The utility model relates to the technical field of cell culture equipment, and particularly provides high-flux honeycomb type automatic cell culture equipment. The high-flux honeycomb type automatic cell culture equipment comprises a first box body, wherein a storage chamber and an operation chamber are arranged in the first box body; the device also comprises a storage component and a first mechanical arm; the storage member is provided to be capable of storing the culture flask; the first mechanical arm is arranged to be capable of rotating circumferentially and moving along the X-axis, Y-axis and Z-axis directions so as to change the position of a culture bottle, a pipette, a freezing tube or a centrifuge tube; the honeycomb incubator includes second box and a plurality of monomer incubator, and a plurality of monomer incubators set up in the second box, and the incubator transports the dolly and is used for transporting the monomer incubator between control room and second box. The independent honeycomb incubator is used for cell culture, so that the cell culture flux is improved, the cell cross contamination is prevented, and the equipment cost is lower while the automatic cell culture is realized.

Description

High-flux honeycomb type automatic cell culture equipment

Technical Field

The utility model relates to the technical field of cell culture equipment, and particularly provides high-flux honeycomb type automatic cell culture equipment.

Background

The existing automatic cell culture equipment is characterized in that a culture solution storage area, an operation area and a cell culture area are arranged in a box body, the cell culture area is a large integral incubator or a culture room for simultaneously culturing cells of a plurality of patients, so that the risk of cross infection among the cells of the patients is greatly improved, if the cells after cross contamination are infused back into the patients, medical accidents are likely to be caused, the cell incubator and the operation room are arranged in the same box body or adjacently, the risk of cross contamination of the cells in the operation process is increased, and in addition, the flux of the cell incubator is limited by the size of the box body due to the fact that the whole incubator is assembled and transported conveniently.

Accordingly, there is a need in the art for a new high-throughput honeycomb automated cell culture apparatus to address the problems of low cell culture throughput and cross-contamination between cells of existing automated culture apparatus.

Disclosure of utility model

The utility model aims to solve the technical problems of low cell culture flux and cross contamination among cells of the existing automatic culture equipment.

In a first aspect, the utility model provides a high throughput honeycomb automated cell culture apparatus comprising a first housing, a honeycomb incubator, and an incubator transfer cart, the first housing having a storage compartment and an operating compartment disposed therein; the high-throughput honeycomb type automatic cell culture equipment further comprises a storage component and a first mechanical arm; the storage member is configured to be capable of storing a culture flask; the first mechanical arm is arranged to be capable of rotating circumferentially and moving along the X-axis, Y-axis and Z-axis directions so as to change the position of a culture bottle, a pipette, a freezing tube or a centrifuge tube; the honeycomb incubator comprises a second incubator body and a plurality of single incubator bodies, wherein the single incubator bodies are arranged in the second incubator body, and the incubator transfer trolley is used for transferring the single incubator bodies between the operation chamber and the second incubator body.

In the preferred technical scheme of the high-flux honeycomb type automatic cell culture equipment, the high-flux honeycomb type automatic cell culture equipment further comprises a discharge gun, a detection chamber is further arranged in the first box body, the discharge gun is arranged in the detection chamber, and the discharge gun is arranged to be capable of injecting culture solution and liquid medicine into the pore plate.

In the preferred technical scheme of the high-flux honeycomb type automatic cell culture equipment, the high-flux honeycomb type automatic cell culture equipment comprises a liquid adding system, wherein the liquid adding system comprises a first peristaltic pump, a liquid inlet end and a liquid outlet end, the liquid inlet end and the liquid outlet end are connected with the first peristaltic pump, the liquid inlet end is positioned in the storage chamber, and the liquid outlet end is positioned in the operation chamber.

In a preferred embodiment of the above high-throughput cellular automatic cell culture apparatus, the high-throughput cellular automatic cell culture apparatus comprises a pipetting system comprising a syringe pump and a pipette, the pipette is connected to the syringe pump, the syringe pump is disposed on top of the operation chamber, and the pipette tip is downward.

In the preferred technical scheme of the high-throughput honeycomb type automatic cell culture equipment, the high-throughput honeycomb type automatic cell culture equipment comprises a vibration incubation device, and the vibration incubation device is arranged in the operation chamber.

In the preferred technical solution of the high-throughput honeycomb-type automatic cell culture apparatus, the high-throughput honeycomb-type automatic cell culture apparatus further comprises a second mechanical arm, the second mechanical arm is arranged in the storage chamber, a transfer platform is arranged between the storage chamber and the operation chamber, and the second mechanical arm is arranged to be capable of rotating circumferentially and moving along the directions of the X axis, the Y axis and the Z axis so as to change the position of the culture flask, the pipette, the cryopreservation tube or the centrifuge tube in the storage chamber.

In the preferred technical scheme of the high-flux honeycomb type automatic cell culture equipment, the high-flux honeycomb type automatic cell culture equipment comprises a collecting device, the collecting device comprises a collecting funnel, a second peristaltic pump and a filling container, the collecting funnel is arranged in the operating chamber, the second peristaltic pump is connected with the collecting funnel through a pipeline, a liquid outlet end of the collecting device is positioned in the storage chamber, and the filling container is arranged below the liquid outlet end of the collecting device.

In the preferred technical solution of the high-throughput honeycomb-type automatic cell culture apparatus, the high-throughput honeycomb-type automatic cell culture apparatus further comprises a cap opener, the cap opener is arranged at the top of the operation chamber, and the cap opener is arranged to be capable of opening a bottle cap of the culture bottle.

In the preferred technical scheme of the high-throughput honeycomb-type automatic cell culture equipment, the second box body further comprises a frame body, a plurality of storage grids are arranged on the frame body, and a plurality of single culture boxes are correspondingly arranged in each storage grid.

In a preferred embodiment of the above high-throughput honeycomb-type automated cell culture apparatus, the high-throughput honeycomb-type automated cell culture apparatus comprises a centrifuge, the centrifuge being disposed within the operation chamber; and/or the high throughput honeycomb automated cell culture apparatus further comprises a centrifuge tube tray for placing a balancing centrifuge tube, a rotating centrifuge tube middle turret for storing a centrifuge tube to be centrifuged, a centrifuge tube box for storing an empty centrifuge tube, and a pipette box for storing an empty pipette; and/or the storage chamber, the operation chamber and the detection chamber are provided with high-efficiency filters, the high-efficiency filters are arranged to discharge the fresh air filtered outdoors into the storage chamber, the operation chamber and the detection chamber, and the air inlet quantity is larger than the air outlet quantity; and/or the high throughput honeycomb automated cell culture apparatus further comprises a microscope disposed within the operating chamber; and/or the high-throughput honeycomb-type automatic cell culture equipment comprises a waste liquid collecting member, wherein the waste liquid collecting member is arranged in the operation chamber, a waste liquid collecting port is formed in the waste liquid collecting member, and the waste liquid collecting member is communicated with the outside of the operation chamber.

As will be appreciated by those skilled in the art, the high throughput, honeycomb automated cell culture apparatus of the present utility model comprises a first housing having a storage compartment and an operating compartment disposed therein, a honeycomb incubator, and an incubator transfer cart;

The high throughput honeycomb automated cell culture apparatus further comprises a storage member, a first robotic arm;

The storage member is provided to be capable of storing the culture flask;

The first mechanical arm is arranged to be capable of rotating circumferentially and moving along the X-axis, Y-axis and Z-axis directions so as to change the position of a culture bottle, a pipette, a freezing tube or a centrifuge tube;

The honeycomb incubator includes second box and a plurality of monomer incubator, and a plurality of monomer incubators set up in the second box, and the incubator transports the dolly and is used for transporting the monomer incubator between control room and second box.

Under the condition of adopting the technical scheme, the high-throughput honeycomb type automatic cell culture equipment disclosed by the utility model adopts the independent honeycomb type incubator to culture cells, wherein the honeycomb type incubator cultures the cells in the form of the single incubator, the cells of different patients are arranged in the independent single incubator to culture, so that cross contamination among the cells during cell culture can be prevented, the honeycomb type incubator is respectively and independently arranged with an operation room and a box body where a storage room is arranged, the throughput of the honeycomb type incubator can be set to be high as required, the honeycomb type incubator can fully utilize the space above the ground, and the single incubator is transported between the honeycomb type incubator and the operation room through the incubator transport trolley, so that cross contamination among the cells in the process of transferring a culture bottle by a mechanical arm in the operation room is further reduced.

Drawings

Preferred embodiments of the present utility model are described below with reference to the accompanying drawings, in which:

FIG. 1 is a schematic diagram of a partial front view of a high throughput honeycomb automated cell culture apparatus of the present utility model;

FIG. 2 is a schematic diagram of a side-looking configuration of a high throughput honeycomb automated cell culture apparatus of the utility model.

List of reference numerals:

1. A first case;

11. a storage room; 111. a storage member;

12. An operation chamber; 121. a first mechanical arm; 122. a liquid adding system; 1221. a liquid outlet end; 123. a pipetting system; 124. vibrating the incubation device; 125. a collecting device; 1251. a collection funnel; 126. a cap opener; 127. a centrifuge; 128. centrifuge tube tray; 129. a microscope; 1211. a waste liquid collection member; 1212. a storage station;

13. a detection chamber; 131. gun arrangement;

14. A third display screen;

2. A second case; 21. a monomer incubator; 22. a frame body; 23. a first display screen;

3. incubator transfer trolley; 31. a second display screen;

4. High efficiency filter.

Detailed Description

Preferred embodiments of the present utility model are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present utility model, and are not intended to limit the scope of the present utility model. Those skilled in the art can adapt it as desired to suit a particular application.

It should be noted that, in the description of the present utility model, terms such as "upper," "lower," "left," "right," "inner," "outer," and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Furthermore, it should be noted that, in the description of the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; may be a mechanical connection; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those skilled in the art according to the specific circumstances.

Referring to fig. 1 and 2, in order to solve the problems of low cell culture flux and cross contamination between cells of the existing automatic culture equipment, the high-flux honeycomb type automatic cell culture equipment of the utility model comprises a first box body 1, a honeycomb type incubator and an incubator transfer trolley 3, wherein a storage chamber 11 and an operation chamber 12 are arranged in the first box body 1;

the high throughput honeycomb automated cell culture apparatus further comprises a storage member 111, a first robotic arm 121;

the storage member 111 is provided to be capable of storing a culture flask;

The first mechanical arm 121 is provided to be capable of rotating circumferentially and moving in the X-axis, Y-axis and Z-axis directions to be capable of changing the position of a flask, pipette, freezing tube or centrifuge tube;

The honeycomb incubator includes a second case 2 and a plurality of individual incubators 21, the plurality of individual incubators 21 are provided in the second case 2, and the incubator transfer cart 3 is used for transferring the individual incubators 21 between the operation room 12 and the second case 2.

The above arrangement has the advantages that: the high-throughput honeycomb type automatic cell culture equipment provided by the utility model adopts the independent honeycomb type incubator to culture cells in the form of the single incubator 21, and cells of different patients are arranged in the independent single incubator 21 to culture, so that cross contamination among cells during cell culture can be prevented, the honeycomb type incubator, the operating room 12 and the box body where the storage room 11 are positioned are respectively and independently arranged, the throughput of the honeycomb type incubator can be set to be high as required, the honeycomb type incubator can fully utilize the space above the ground, and the single incubator 21 is transported between the honeycomb type incubator and the operating room 12 through the incubator transport trolley 3, so that cross contamination among cells in the process of transferring the culture bottles by the mechanical arm in the operating room is further reduced.

Referring to fig. 1, in one possible embodiment, a storage chamber 11, an operation chamber 12 and a detection chamber 13 are provided in a first case 1, the storage chamber 11 is provided at the left side of the operation chamber 12, the detection chamber 13 is provided at the rear side of the operation chamber 12, a honeycomb type incubator is provided at the right side of the operation chamber 12, sterilizing devices (not shown) are provided in the storage chamber 11, the operation chamber 12 and the detection chamber 13, high efficiency filters 4 are provided at the top of the storage chamber 11, the operation chamber 12 and the detection chamber 13, the high efficiency filters 4 are provided to be capable of feeding filtered outdoor fresh air into the storage chamber 11, the operation chamber 12 and the detection chamber 13, and the air inlet amount is set to be larger than the air outlet amount, so that the storage chamber 11, the operation chamber 12 and the detection chamber 13 are always kept in a positive pressure state, and outdoor dirty air is prevented from entering the first case 1.

With continued reference to fig. 1 and 2, further, the honeycomb incubator includes a second box 2, a frame 22 and a plurality of single incubators 21, the frame 22 is disposed in the second box 2, a plurality of storage grids are disposed on the frame 22, and the plurality of single incubators 21 are correspondingly disposed in the respective storage grids; the planking of second box 2 is provided with first display screen 23, and first display screen 23 is used for showing information such as temperature, quantity of monomer incubator 21 in the second box 2, is provided with second display screen 31 on the incubator transportation dolly 3, and second display screen 31 is used for showing the state of operation dolly. A third display screen is provided on the first casing 1 to display status information such as temperature, humidity, etc. inside the first casing 1.

Further, the high throughput honeycomb automated cell culture apparatus further comprises a storage member 111, a first mechanical arm 121, a second mechanical arm (not shown), a liquid feeding system 122, a liquid moving system 123, a shake incubation device 124, a collection device 125, a cap opener 126, a centrifuge 127, and a gun 131.

Specifically, a lance 131 is provided in the detection chamber 13, and the lance 131 is provided so as to be able to inject a culture solution and a drug into the well plate to test the response of cells to the drug.

The storage member 111 is provided to be able to store a culture flask, a centrifuge tube, a pipette, and a freezing tube. Optionally, the storage member 111 includes a first storage member rotatable in a circumferential direction, the culture flask is disposed on the first storage member, the storage member 111 further includes a second storage member, a third storage member, and a fourth storage member, and the freezing tube is disposed on the second storage member; the centrifuge tube is arranged on the third storage component; the pipette is disposed on the fourth storage member. The first mechanical arm 121 is conveniently taken and placed by storing culture bottles, centrifuge tubes, pipettes and freezing tubes in a partitioned manner.

The first mechanical arm 121 is disposed in the operation chamber 12, the first mechanical arm 121 is disposed so as to be capable of rotating circumferentially and moving along the X-axis, Y-axis and Z-axis directions so as to change the position of the flask, pipette, freezing tube or centrifuge tube, the operation chamber 12 is provided with an openable sealing window and a sterilizing liquid spraying system, and the sterilizing liquid spraying system is disposed so as to be capable of sterilizing and disinfecting the monomer incubator 21. The incubator transfer trolley 3 carries the single incubator 21 and moves to the sealing window, the sealing window is opened, the sterilizing liquid spraying system sterilizes the single incubator 21, the first mechanical arm 121 takes away the culture bottles in the single incubator 21, and the sealing window is closed.

The second mechanical arm is arranged in the storage chamber 11, a transfer platform is arranged between the storage chamber 11 and the operation chamber 12, the second mechanical arm is also arranged to be capable of rotating circumferentially and moving along the X-axis, the Y-axis and the Z-axis directions so as to change the positions of the culture flask, the pipette, the freezing tube or the centrifuge tube in the storage chamber 11, the mechanical clamping jaw of the second mechanical arm transfers the culture flask, the pipette, the freezing tube or the centrifuge tube to the transfer platform, and the mechanical clamping jaw of the first mechanical arm 121 transfers the mechanical clamping jaw from the transfer platform to other positions, so that the moving distance of the first mechanical arm 121 is reduced, and the volume of the first mechanical arm 121 is reduced. However, the transfer platform may not be disposed between the storage chamber 11 and the operation chamber 12, and the first mechanical arm 121 may directly take the flask from the second mechanical arm, so that a person skilled in the art may set the flask according to the need.

The liquid adding system 122 comprises a first peristaltic pump, a liquid inlet end and a liquid outlet end 1221, wherein the liquid inlet end and the liquid outlet end are connected with a pipeline of the first peristaltic pump, the liquid inlet end is positioned in the storage chamber 11, a storage table 1212 is arranged below the liquid inlet end, a culture bottle corresponding to the liquid inlet end is arranged on the storage table 1212, and the liquid outlet end 1221 is positioned in the operation chamber 12 so as to pump culture liquid in the culture bottle in the storage chamber 11 into the culture bottle of the liquid outlet end 1221. Optionally, the number of the liquid inlet ends and the liquid outlet ends 1221 is the same, and each liquid inlet end corresponds to one culture bottle, different culture liquids are filled in the culture bottles, and after the first mechanical arm 121 clamps the culture bottles to the liquid outlet ends 1221, the corresponding culture liquid can be injected into the culture bottles on the first mechanical arm 121 as required.

The collecting device 125 includes a second peristaltic pump, a collecting funnel 1251, and a filling container, the collecting funnel 1251 is disposed in the operation chamber 12, the second peristaltic pump is connected with the collecting funnel 1251 through a pipeline, a liquid outlet end 1221 of the collecting device 125 is located in the storage chamber 11, the first mechanical arm 121 pours the culture solution in the culture flask into the collecting funnel 1251, and the filling container is disposed below the liquid outlet end 1221 of the collecting device 125 to fill the culture solution into the filling container.

The centrifuge 127 is disposed within the operating chamber 12, and the high throughput, honeycomb automated cell culture apparatus further includes a centrifuge tube tray 128 for holding balancing centrifuge tubes, a rotating centrifuge tube turret for holding centrifuge tubes to be centrifuged, a centrifuge tube cassette for holding emptying centrifuge tubes, and a pipette cassette for holding emptying pipettes.

An oscillation incubation device 124 is provided in the operation chamber 12 for changing the cells in an adherent state into a suspension state by oscillation. Specifically, pancreatin is added into the cell culture solution to digest mucin of the cells, and then the adherent cells are oscillated by means of the impact force generated by the oscillation of the oscillation incubation device 124, so that the suspension is prepared with less time, and the contact time of the cells and mucin is less, thereby better protecting the cells from being destroyed.

The pipetting system 123 is arranged in the operation room 12, and the pipetting system 123 comprises a syringe pump and a pipette, the pipette is connected with the syringe pump, the syringe pump is arranged at the top of the operation room 12, and the pipette tip is downward and is used for transferring the culture solution in one culture flask to another culture flask.

The high-throughput honeycomb-type automated cell culture apparatus further comprises a microscope 129 and a waste liquid collecting member 1211, the microscope 129, the cap opener 126 and the waste liquid collecting member 1211 being disposed in the operation chamber 12, the microscope 129 being for observing the cell number, contamination rate, etc. of the cultured cells. Optionally, a lid opener 126 is provided on top of the operating room 12, the lid opener 126 being used to open the caps of the flasks, centrifuge tubes or freezer tubes. The waste liquid collecting member 1211 is provided with a waste liquid collecting port which communicates with the outside of the operation chamber 12 to discharge the waste liquid in the operation chamber 12 from the waste liquid collecting port to the operation chamber 12.

In summary, the high-throughput honeycomb-type automatic cell culture apparatus of the present utility model increases the cell culture throughput by providing the independent honeycomb-type incubator and increases the detection chamber 13, and the cells after centrifugation and shake incubation treatment in the operation chamber 12 enter the detection chamber 13 to perform drug detection, thereby realizing automation of cell collection, treatment, culture and drug detection.

The foregoing embodiments are merely illustrative of the principles of the present utility model, and are not intended to limit the scope of the utility model, as those skilled in the art can modify the above structure without departing from the principles of the present utility model, so that the present utility model can be applied to more specific application scenarios.

Thus far, the technical solution of the present utility model has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present utility model is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present utility model, and such modifications and substitutions will fall within the scope of the present utility model.

Claims (10)

1. The high-throughput honeycomb type automatic cell culture equipment is characterized by comprising a first box body, a honeycomb type incubator and an incubator transfer trolley, wherein a storage room and an operation room are arranged in the first box body;

The high-throughput honeycomb type automatic cell culture equipment further comprises a storage component and a first mechanical arm;

the storage member is configured to be capable of storing a culture flask;

The first mechanical arm is arranged to be capable of rotating circumferentially and moving along the X-axis, Y-axis and Z-axis directions so as to change the position of a culture bottle, a pipette, a freezing tube or a centrifuge tube;

The honeycomb incubator comprises a second incubator body and a plurality of single incubator bodies, wherein the single incubator bodies are arranged in the second incubator body, and the incubator transfer trolley is used for transferring the single incubator bodies between the operation chamber and the second incubator body.

2. The high-throughput, honeycomb automated cell culture apparatus of claim 1, further comprising a lance, the first housing further having a detection chamber disposed therein, the lance being configured to inject culture fluid and medical fluid into the well plate.

3. The high-throughput, honeycomb automated cell culture apparatus of claim 1, comprising a liquid feeding system comprising a first peristaltic pump and a liquid inlet end and a liquid outlet end connected to the first peristaltic pump, the liquid inlet end being located within the storage chamber and the liquid outlet end being located within the operating chamber.

4. The high-throughput cellular automation cell culture apparatus of claim 1, comprising a pipetting system comprising a syringe pump and a pipette, the pipette connected to the syringe pump, the syringe pump disposed on top of the operating chamber, the pipette tip facing downward.

5. The high-throughput, honeycomb automated cell culture apparatus of claim 1, comprising an shake incubation device disposed within the operating chamber.

6. The high-throughput cellular automation cell culture apparatus of claim 1, further comprising a second robotic arm disposed within the storage chamber, a staging platform disposed between the storage chamber and the handling chamber, the second robotic arm configured to be rotatable circumferentially and movable in X-axis, Y-axis and Z-axis directions to enable changing the position of a flask, pipette, cryopreservation tube or centrifuge tube within the storage chamber.

7. The high-throughput cellular automated cell culture apparatus of claim 1, comprising a collection device comprising a collection funnel disposed within the operating chamber, a second peristaltic pump in communication with the collection funnel, a liquid outlet end of the collection device disposed within the storage chamber, and a filling container disposed below the liquid outlet end of the collection device.

8. The high-throughput, automated cellular cell culture apparatus of any one of claims 1-7, further comprising a cap opener disposed on top of the operating chamber, the cap opener configured to enable opening of a cap of a culture flask.

9. The high throughput, honeycomb automated cell culture apparatus of any one of claims 1-7, wherein the second housing further comprises a frame having a plurality of storage compartments disposed thereon, a plurality of the monomer incubators being disposed within each of the storage compartments, respectively.

10. The high-throughput, honeycomb automated cell culture apparatus of claim 2, wherein the high-throughput, honeycomb automated cell culture apparatus comprises a centrifuge disposed within the operating chamber; and/or the high throughput honeycomb automated cell culture apparatus further comprises a centrifuge tube tray for placing a balancing centrifuge tube, a rotating centrifuge tube middle turret for storing a centrifuge tube to be centrifuged, a centrifuge tube box for storing an empty centrifuge tube, and a pipette box for storing an empty pipette; and/or the storage chamber, the operation chamber and the detection chamber are provided with high-efficiency filters, the high-efficiency filters are arranged to discharge the fresh air filtered outdoors into the storage chamber, the operation chamber and the detection chamber, and the air inlet quantity is larger than the air outlet quantity; and/or the high throughput honeycomb automated cell culture apparatus further comprises a microscope disposed within the operating chamber; and/or the high-throughput honeycomb-type automatic cell culture equipment comprises a waste liquid collecting member, wherein the waste liquid collecting member is arranged in the operation chamber, a waste liquid collecting port is formed in the waste liquid collecting member, and the waste liquid collecting member is communicated with the outside of the operation chamber.