CN220976952U - Adsorption device for amylase immobilization - Google Patents

Adsorption device for amylase immobilization Download PDF

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Publication number
CN220976952U
CN220976952U CN202322346510.1U CN202322346510U CN220976952U CN 220976952 U CN220976952 U CN 220976952U CN 202322346510 U CN202322346510 U CN 202322346510U CN 220976952 U CN220976952 U CN 220976952U
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China
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reaction tank
tank body
fixedly connected
amylase
wall
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CN202322346510.1U
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Chinese (zh)
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时杰
杨浩文
何忠琳
赵保柱
李正伟
孙国升
郑磊
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Hefei University of Technology
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Hefei University of Technology
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Abstract

The utility model relates to the technical field of amylase immobilization, in particular to an adsorption device for amylase immobilization, which comprises a reaction tank body, a stirring mechanism arranged in the reaction tank body, and a sampling mechanism fixedly arranged at one side of the reaction tank body; the stirring mechanism is used for mixing and stirring materials in the reaction tank body, and the sampling mechanism is used for conveniently sampling the materials in the reaction tank body; the reaction tank body is provided with a feed hopper, a water inlet pipe and a discharge pipe; the bottom of the hopper penetrates through the top of the reaction tank body and is communicated with the inside of the reaction tank body; the water inlet pipe is arranged at a position close to the upper end of the reaction tank body and is communicated with the interior of the reaction tank body; the discharge pipe is arranged at a position close to the bottom of the reaction tank body and is communicated with the inside of the reaction tank body. According to the utility model, the stirring mechanism and the sampling mechanism are arranged, so that materials in the reaction tank body can be conveniently stirred, and the liquid in the adsorption device can be conveniently sampled and detected.

Description

Adsorption device for amylase immobilization
Technical Field
The utility model relates to the technical field of amylase immobilization, in particular to an adsorption device for amylase immobilization.
Background
Currently, immobilized enzymes are agents in which water-soluble enzymes are physically or chemically immobilized on a medium to render them water-insoluble and enzymatically active. The immobilized enzyme can improve the stability of the enzyme, can be stored and used for a long time (can resist the change of temperature and PH), can be repeatedly used, is more economical and beneficial to production, and can be contacted with reactants and separated and purified with products.
Immobilization of amylase includes adsorption, covalent coupling, crosslinking and embedding, and the adsorption is a method of adsorbing enzyme or enzyme-containing bacteria on its surface by various adsorbents, typically physical adsorption and ion adsorption, and commonly used adsorbents include activated carbon, alumina, porous ceramics, and the like. In order to detect whether the solution contains unadsorbed free amylase or not in the amylase immobilization experiment, the supernatant is required to be taken for a plurality of times to carry out drip detection in a test tube, so that whether the unadsorbed free amylase is washed clean or not is detected, and the conventional amylase immobilization adsorption device is inconvenient to detect the free amylase;
there is a need to provide an adsorption device that facilitates sampling and detection.
Disclosure of utility model
Based on the above description, the utility model provides an adsorption device for amylase immobilization, which is convenient for stirring materials in a reaction tank body by arranging a stirring mechanism and a sampling mechanism so as to sample and detect liquid in the adsorption device.
The technical scheme for solving the technical problems is as follows: an amylase immobilization adsorption device comprises a reaction tank body, a stirring mechanism arranged in the reaction tank body, and a sampling mechanism fixedly arranged on one side of the reaction tank body;
The stirring mechanism is used for mixing and stirring materials in the reaction tank body, and the sampling mechanism is used for conveniently sampling the materials in the reaction tank body;
The reaction tank body is provided with a feed hopper, a water inlet pipe and a discharge pipe;
The bottom of the hopper penetrates through the top of the reaction tank body and is communicated with the inside of the reaction tank body;
The water inlet pipe is arranged at a position close to the upper end of the reaction tank body, and is communicated with the interior of the reaction tank body;
The discharge pipe is arranged at a position close to the bottom of the reaction tank body and is communicated with the interior of the reaction tank body.
Through above-mentioned technical scheme, rabbling mechanism is used for carrying out the mixing to the internal material of retort, and sampling mechanism is used for convenient material to the internal material of retort to detect the material after mixing.
On the basis of the technical scheme, the utility model can be improved as follows.
Further, the stirring mechanism comprises a driving motor, a transmission assembly, a stirring rod and a scraping plate, and the driving motor is fixedly arranged at the bottom of the reaction tank body;
The transmission assembly comprises a first gear and a second gear which are fixedly connected with the main shaft of the driving motor, the first gear is meshed with the second gear, and a rotating shaft is coaxially and fixedly connected at the axle center of the second gear;
The rotating shaft penetrates through the bottom of the reaction tank body and is rotationally connected with the reaction tank body;
The bottom and the pivot fixed connection of puddler, scraper blade fixed connection is close to the one end of the internal wall of retort at the puddler, and the side of scraper blade is laminated with the inner wall butt of the retort.
Further, the sampling mechanism comprises a U-shaped tube and a glass tube which are connected with the reaction tank body, and the U-shaped tube is arranged upside down;
The outer part of the U-shaped pipe is sleeved with a glass pipe, the inner part of the U-shaped pipe is sleeved with a piston block in a sliding manner, the bottom of the piston block is fixedly connected with a piston column, and the bottom end of the piston column is fixedly connected with a stirring block;
The outer wall bottom fixedly connected with of glass pipe is with the rubber tube that is linked together rather than inside, fixedly connected with is connected with the liquid column that advances that is linked together rather than inside on the outer wall of glass pipe, the inside slip of liquid column has cup jointed the rubber head burette.
Further, sliding connection has the sliding plate on the outer wall of the retort body, fixedly connected with fluid-discharge tube on the outer wall of sliding plate, fixedly connected with lifter plate on the outer wall of fluid-discharge tube, the screw rod has been cup jointed in the rotation on the lifter plate, the top fixedly connected with rotation handle of screw rod, the both ends of screw rod all rotate be connected with retort body fixedly connected's fixing base.
Further, two transparent plates are symmetrically and fixedly arranged on the outer wall of the reaction tank body around the vertical center plane of the reaction tank body.
Further, two stirring rods are arranged, two stirring rods are symmetrically arranged, and two stirring rods are arranged in a V-shaped mode.
Further, the inside sliding sleeve of one end of U type pipe has cup jointed the sliding tube, fixedly connected with electric pole on the outer wall of sliding tube, electric pole and retort body roof fixed connection.
Through the technical scheme, the electric rod is used for pushing the sliding pipe to move so that the sliding pipe can be communicated with different heights of the reaction tank body, and therefore liquid with different heights can be sampled.
Compared with the prior art, the technical scheme of the application has the following beneficial technical effects:
1. The amylase and distilled water are poured into the feed hopper, then Xu Danying sand can be added into the reaction tank body, the quartz sand is used as an adsorbent, and has the advantages of hardness, wear resistance, stable performance and low danger, so that the amylase is suitable for immobilization, in order to improve the amylase immobilization, the output end of the driving motor can drive the gear I and the gear II to rotate in a meshed manner, the rotation speed of the rotating shaft can be controlled to be slower due to the fact that the transmission ratio between the gear I and the gear II is larger than 1, the stirring force is smaller, quartz sand can be prevented from being ground and penetrating into effluent liquid through the filtering belt, and the solidification adsorption rate of amylase can be improved;
2. the height of the supernatant in the solution in the reaction tank body can be observed through the transparent plate, then the screw rod can be driven to rotate through the rotating handle, so that the height of the liquid discharge pipe can be adjusted, the supernatant in the reaction tank body can be fed and discharged through the liquid discharge pipe, then quartz sand is cleaned through distilled water, the sliding pipe can be driven to descend into the supernatant through the electric rod in the sampling mechanism, then the piston column is driven to slide onto the inner bottom surface of the glass pipe through the stirring block, so that the supernatant in the reaction tank body can be pumped into the glass pipe, then the starch solution and one to two drops of potassium iodide solution can be dripped into the liquid inlet column through the rubber head dropper, the color of the solution in the glass pipe is observed again, if the color of the solution is blue, the fact that no free amylase exists in the reaction tank body is indicated, if the solution is red, the solution is continuously cleaned through distilled water until the starch solution becomes blue, and thus the adsorption method experiment of amylase immobilization can be conveniently carried out.
Drawings
FIG. 1 is a schematic view of the overall structure of the present utility model;
FIG. 2 is a schematic diagram of the stirring mechanism in the utility model;
FIG. 3 is a schematic view of the internal structure of a reaction tank according to the present utility model;
FIG. 4 is a schematic diagram of a sampling mechanism according to the present utility model;
FIG. 5 is a schematic cross-sectional view of a sampling mechanism according to the present utility model.
Reference numerals: 100. a stirring mechanism; 101. a reaction tank body; 102. a feed hopper; 103. a water inlet pipe; 104. a discharge pipe; 105. a transparent plate; 106. a sliding plate; 107. a liquid discharge pipe; 108. a lifting plate; 109. a screw; 110. rotating the handle; 111. a driving motor; 112. a first gear; 113. a second gear; 114. a rotating shaft; 115. a stirring rod; 116. a scraper; 200. a sampling mechanism; 201. a U-shaped tube; 202. a sliding tube; 203. an electric lever; 204. a glass tube; 205. a piston block; 206. a piston column; 207. a poking block; 208. a guide post; 209. a liquid inlet column; 210. a rubber head dropper; 211. and (5) a rubber tube.
Detailed Description
In order that the application may be readily understood, a more complete description of the application will be rendered by reference to the appended drawings. Embodiments of the application are illustrated in the accompanying drawings. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
Examples:
Referring to fig. 1 to 5, an amylase immobilization adsorption apparatus includes a reaction tank 101, a stirring mechanism 100 provided in the reaction tank 101, and a sampling mechanism 200 fixedly provided at one side of the reaction tank 101; the stirring mechanism 100 is used for mixing and stirring materials in the reaction tank 101, and the sampling mechanism 200 is used for conveniently sampling the materials in the reaction tank 101 so as to detect the mixed materials.
Referring to fig. 1 to 5, a feed hopper 102, a water inlet pipe 103 and a discharge pipe 104 are connected to a solid on a reaction tank 101, and the bottom of the feed hopper 102 penetrates through the top of the reaction tank 101 and is communicated with the inside of the reaction tank 101; the water inlet pipe 103 is arranged at a position close to the upper end of the reaction tank body 101, and the water inlet pipe 103 is communicated with the interior of the reaction tank body 101; the discharge pipe 104 is arranged at a position close to the bottom of the reaction tank 101, and the discharge pipe 104 is communicated with the inside of the reaction tank 101; solenoid valves for opening and closing the pipe body are arranged on the water inlet pipe 103 and the water outlet pipe 104.
Referring to fig. 1 to 5, the stirring mechanism 100 includes a driving motor 111, a transmission assembly, a stirring rod 115, and a scraper 116, wherein the driving motor 111 is fixedly disposed at the bottom of the reaction tank 101; the transmission assembly comprises a first gear 112 and a second gear 113 which are fixedly connected with a main shaft of the driving motor 111, the first gear 112 is meshed with the second gear 113, and a rotating shaft 114 is coaxially and fixedly connected at the axis of the second gear 113; the rotating shaft 114 penetrates through the bottom of the reaction tank 101 and is rotatably connected with the reaction tank 101; the two stirring rods 115 are symmetrically arranged, the two stirring rods 115 are arranged in a V shape, and the bottoms of the two stirring rods 115 are fixedly connected with the rotating shaft 114; the scraper 116 is fixedly connected to one end of the stirring rod 115, which is close to the inner wall of the reaction tank 101, and the side edge of the scraper 116 is abutted against the inner wall of the reaction tank 101.
Referring to fig. 1-5, after the driving motor 111 is started, the first driving gear 112 rotates, so as to drive the rotating shaft 114 to rotate, and further drive the scraping plate 116 to rotate, and the scraping plate 116 can scrape off quartz sand on the inner wall of the reaction tank 101, so that the phenomenon of wall hanging of the quartz sand is avoided.
Referring to fig. 1-5, a sliding plate 106 is slidably connected to the outer wall of the reaction tank 101, a drain pipe 107 is fixedly connected to the outer wall of the sliding plate 106, a lifting plate 108 is fixedly connected to the outer wall of the drain pipe 107, a screw 109 is screwed and sleeved on the lifting plate 108, a rotating handle 110 is fixedly connected to the top end of the screw 109, fixing seats fixedly connected with the reaction tank 101 are rotatably connected to the two ends of the screw 109, and the screw 109 can be driven to rotate by rotating the handle 110, so that the sliding plate 106 can be driven to slide on the outer wall of the reaction tank 101, and the height of the drain pipe 107 can be adjusted;
The outer wall of the reaction tank body 101 is symmetrically provided with observation windows about the vertical center plane, two transparent plates 105 are fixedly arranged at the observation windows, and the solution layering condition inside the reaction tank body 101 can be conveniently observed by experimenters through the transparent plates 105.
Referring to fig. 1 to 5, the sampling mechanism 200 includes a U-shaped tube 201 and a glass tube 204 fixedly connected to the reaction tank 101, the U-shaped tube 201 being disposed upside down; one end of the U-shaped pipe 201 is arranged inside the reaction tank body 101, and the other end is arranged outside the reaction tank body 101; the glass tube 204 is sleeved at one end of the outer part of the reaction tank body 101 of the U-shaped tube 201, the glass tube 204 is fixedly connected with the outer wall of the reaction tank body 101, a piston block 205 is sleeved in the U-shaped tube 201 in a sliding manner, a piston column 206 is fixedly connected to the bottom of the piston block 205, a stirring block 207 is fixedly connected to the bottom end of the piston column 206, two guide columns 208 which are in sliding connection with the glass tube 204 are symmetrically and fixedly connected to the vertical center plane of the piston column 206, a rubber tube 211 which is communicated with the inner part of the glass tube 204 is fixedly connected to the bottom end of the outer wall of the glass tube 204, a liquid inlet column 209 which is communicated with the inner part of the glass tube 204 is fixedly connected to the outer wall of the glass tube 204, and a rubber head dropper 210 is sleeved in the inner part of the liquid inlet column 209 in a sliding manner, so that the detection of the completion degree of amylase immobilization in experiments can be conveniently carried out through the sampling mechanism 200; the inside slip sleeve joint slip pipe 202 of one end of U type pipe 201, fixedly connected with electric rod 203 on the outer wall of slip pipe 202, electric rod 203 and retort body 101 roof fixed connection can drive the bottom of slip pipe 202 through electric rod 203 and stretch into in the supernatant and conveniently draw out the detection.
Working principle:
when the amylase and distilled water stirring device is used, a certain amount of amylase and distilled water can be poured into the feed hopper 102, then Xu Danying sand can be added into the reaction tank 101, and quartz sand has the advantages of being hard, wear-resistant, stable in performance and low in danger as an adsorbent, so that the amylase is suitable for immobilization;
The height of the supernatant in the solution in the reaction tank 101 can be observed through the transparent plate 105, then the screw 109 can be driven to rotate through the rotating handle 110, so that the height of the liquid discharge pipe 107 can be adjusted, the supernatant in the reaction tank 101 can be fed and discharged through the liquid discharge pipe 107, and then the quartz sand is cleaned through distilled water; the electric rod 203 in the sampling mechanism 200 can drive the sliding tube 202 to descend into the supernatant, then the stirring block 207 drives the piston column 206 to slide onto the inner bottom surface of the glass tube 204, so that the supernatant in the reaction tank 101 can be pumped into the glass tube 204, then starch solution and one to two drops of potassium iodide solution can be dripped into the liquid inlet column 209 through the rubber head dropper 210, and then the color of the solution in the glass tube 204 is observed; if the color of the solution is blue, which indicates that no amylase is released in the reaction tank 101, if the solution is red, the solution is washed with distilled water until the starch solution turns blue, thereby facilitating the adsorption method experiment of amylase immobilization.
The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.

Claims (7)

1. An amylase immobilization adsorption device is characterized by comprising a reaction tank body (101), a stirring mechanism (100) arranged in the reaction tank body (101), and a sampling mechanism (200) fixedly arranged on one side of the reaction tank body (101);
The stirring mechanism (100) is used for mixing and stirring materials in the reaction tank body (101), and the sampling mechanism (200) is used for conveniently sampling the materials in the reaction tank body (101);
A feed hopper (102), a water inlet pipe (103) and a discharge pipe (104) are arranged on the reaction tank body (101);
The bottom of the hopper penetrates through the top of the reaction tank body (101) and is communicated with the inside of the reaction tank body (101);
The water inlet pipe (103) is arranged at a position close to the upper end of the reaction tank body (101), and the water inlet pipe (103) is communicated with the inside of the reaction tank body (101);
the discharge pipe (104) is arranged at a position close to the bottom of the reaction tank body (101), and the discharge pipe (104) is communicated with the inside of the reaction tank body (101).
2. The amylase immobilization adsorption apparatus according to claim 1, wherein the stirring mechanism (100) comprises a driving motor (111), a transmission assembly, a stirring rod (115) and a scraper (116), and the driving motor (111) is fixedly arranged at the bottom of the reaction tank (101);
The transmission assembly comprises a first gear (112) and a second gear (113) which are fixedly connected with a main shaft of the driving motor (111), the first gear (112) is meshed with the second gear (113), and a rotating shaft (114) is coaxially and fixedly connected at the axis of the second gear (113);
The rotating shaft (114) penetrates through the bottom of the reaction tank body (101) and is rotationally connected with the reaction tank body (101);
the bottom of puddler (115) and pivot (114) fixed connection, scraper blade (116) fixed connection is in the one end of puddler (115) near the inner wall of retort body (101), and the side of scraper blade (116) is laminated with the inner wall butt of retort body (101).
3. The amylase immobilization adsorption apparatus according to claim 1, wherein the sampling mechanism (200) comprises a U-shaped tube (201) and a glass tube (204) connected to the reaction tank (101), the U-shaped tube (201) being disposed upside down;
The outer part of the U-shaped pipe (201) is sleeved with a glass pipe (204), the inner part of the U-shaped pipe (201) is sleeved with a piston block (205) in a sliding manner, the bottom of the piston block (205) is fixedly connected with a piston column (206), and the bottom end of the piston column (206) is fixedly connected with a stirring block (207);
The glass tube is characterized in that a rubber tube (211) communicated with the inside of the glass tube is fixedly connected to the bottom end of the outer wall of the glass tube (204), a liquid inlet column (209) communicated with the inside of the glass tube is fixedly connected to the outer wall of the glass tube (204), and a rubber head dropper (210) is sleeved in the liquid inlet column (209) in a sliding manner.
4. The amylase immobilization adsorption device according to claim 1, wherein a sliding plate (106) is slidably connected to the outer wall of the reaction tank body (101), a liquid discharge pipe (107) is fixedly connected to the outer wall of the sliding plate (106), a lifting plate (108) is fixedly connected to the outer wall of the liquid discharge pipe (107), a screw (109) is rotatably sleeved on the lifting plate (108), a rotary handle (110) is fixedly connected to the top end of the screw (109), and fixing seats fixedly connected with the reaction tank body (101) are rotatably connected to the two ends of the screw (109).
5. The amylase immobilization adsorption apparatus according to claim 1, wherein two transparent plates (105) are fixedly provided on the outer wall of the reaction tank (101) symmetrically with respect to the vertical center plane thereof.
6. The amylase immobilization adsorption apparatus according to claim 2, wherein two stirring rods (115) are provided, the two stirring rods (115) are symmetrically arranged, and the two stirring rods (115) are V-shaped.
7. The amylase immobilization adsorption device according to claim 3, wherein a sliding tube (202) is sleeved in one end of the U-shaped tube (201) in a sliding manner, an electric rod (203) is fixedly connected to the outer wall of the sliding tube (202), and the electric rod (203) is fixedly connected with the top plate of the reaction tank body (101).
CN202322346510.1U 2023-08-30 2023-08-30 Adsorption device for amylase immobilization Active CN220976952U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202322346510.1U CN220976952U (en) 2023-08-30 2023-08-30 Adsorption device for amylase immobilization

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202322346510.1U CN220976952U (en) 2023-08-30 2023-08-30 Adsorption device for amylase immobilization

Publications (1)

Publication Number Publication Date
CN220976952U true CN220976952U (en) 2024-05-17

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ID=91042794

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202322346510.1U Active CN220976952U (en) 2023-08-30 2023-08-30 Adsorption device for amylase immobilization

Country Status (1)

Country Link
CN (1) CN220976952U (en)

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