CN220951847U - Liquid adding device for cell culture - Google Patents
Liquid adding device for cell culture Download PDFInfo
- Publication number
- CN220951847U CN220951847U CN202420600682.9U CN202420600682U CN220951847U CN 220951847 U CN220951847 U CN 220951847U CN 202420600682 U CN202420600682 U CN 202420600682U CN 220951847 U CN220951847 U CN 220951847U
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- liquid
- top end
- cell culture
- fixedly arranged
- base
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- 239000007788 liquid Substances 0.000 title claims abstract description 113
- 238000004113 cell culture Methods 0.000 title claims abstract description 42
- 230000007246 mechanism Effects 0.000 claims abstract description 58
- 230000005540 biological transmission Effects 0.000 claims abstract description 52
- 230000001681 protective effect Effects 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims description 21
- 238000007599 discharging Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 3
- 239000000945 filler Substances 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a liquid feeder for cell culture, in particular to the technical field of cell culture, which comprises a base, wherein a plurality of liquid feeding mechanisms are fixedly arranged in the middle of the top end of the base, a transmission mechanism is fixedly arranged at the top end of the base, a plurality of culture dishes which are uniformly distributed are arranged on the transmission mechanism, and a protective frame is fixedly arranged at the top end of the base at the position of the plurality of liquid feeding mechanisms; the utility model discloses a multi-item liquid adding mechanism, which comprises a mechanism underframe, wherein the mechanism underframe is fixedly arranged in the middle of the top end of a base, a rotating shaft is arranged in the middle of the top end of the mechanism underframe, and a rotating disc is fixedly arranged at the top end of the rotating shaft.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a liquid adding device for cell culture.
Background
In the cell culture process, cell culture liquid needs to be filled into the culture dish to guarantee normal cell culture, cell culture liquid is filled into the traditional cell culture process, the manual filling is carried out mostly, the handheld liquid feeder is aligned to the culture dish, the cell culture liquid is discharged from the liquid feeder and is led into the culture dish by pressing, the manual filling mode can also be used for cell culture with small quantity, but when the cell culture with large quantity and various impurities is carried out, the operation amount is large, the operation intensity of experimenters is increased, the liquid feeding efficiency is low, and the liquid feeding is easy to make mistakes.
Disclosure of utility model
The utility model aims to provide a liquid adding device for cell culture, which solves the problems in the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the liquid feeder for cell culture comprises a base, wherein a plurality of liquid feeding mechanisms are fixedly arranged in the middle of the top end of the base, a transmission mechanism is fixedly arranged at the top end of the base, a plurality of culture dishes which are uniformly distributed are arranged on the transmission mechanism, and a protective frame is fixedly arranged at the top end of the base at the position of the plurality of liquid feeding mechanisms;
The multiple liquid adding mechanisms comprise mechanism underframes, the mechanism underframes are fixedly arranged in the middle of the top end of a base, a rotating shaft is arranged in the middle of the top end of the mechanism underframe, a rotating disc is fixedly arranged at the top end of the rotating shaft, a plurality of support rods distributed in an annular array are fixedly arranged outside the rotating disc, liquid adding pieces are arranged at one ends of the support rods, far away from the rotating disc, of the support rods, a lifting sleeve frame is rotatably arranged in the middle of the top end of the mechanism underframe, lifting inner rods are arranged in the middle of the lifting sleeve frame in a sliding manner, and the bottom ends of the rotating shaft and the top ends of the lifting inner rods are fixedly arranged;
The liquid adding part comprises a liquid storage barrel, the liquid adding part is movably clamped at one end of a corresponding support rod, which is far away from the rotating disc, a liquid discharging nozzle is fixedly arranged at the bottom end of the liquid storage barrel, a sealing cover is arranged at the top end of the liquid storage barrel, a connecting pipe is fixedly arranged in the middle of the top end of the sealing cover, and a pump is fixedly arranged at the top end of the connecting pipe.
Preferably, the outside fixed cover of reservoir is equipped with the holding ring, the bottom fixed mounting of holding ring has the reference column, the movable joint of reference column is on the bracing piece.
Preferably, the top fixing clip of the liquid storage barrel is provided with a threaded pipe, the threaded pipe is installed in the sealing cover in a threaded manner, the sealing cover is provided with a sealing ring in a movable clip manner, and the top end of the threaded pipe is contacted with the lower surface of the sealing ring.
Preferably, the fixed cover in outside bottom of lifting sleeve frame is equipped with first worm wheel, the inner wall upper surface fixed mounting of mechanism underframe has first motor, the driving end fixed mounting of first motor has first worm, first worm and first worm wheel meshing are connected, the inner wall lower surface fixed mounting of mechanism underframe has the lifter, the drive end of lifter passes through the bearing and rotates the bottom middle part of installing at the lifter.
Preferably, the transmission mechanism comprises two groups of transmission side frames which are symmetrically distributed, the transmission side frames are fixedly arranged at the top end of the base, the top of each transmission side frame is rotatably provided with a transmission roller, the outer sides of the two transmission rollers are movably sleeved with transmission belts, the surfaces of the transmission belts are fixedly provided with positioning blocks, the culture dishes are movably clamped in the positioning blocks, and the lower surfaces of the culture dishes are in contact with the upper surfaces of the transmission belts.
Preferably, a second worm wheel is fixedly arranged at the end part of one of the transmission rollers, a second motor is fixedly arranged at the outer side of the transmission side frame, a second worm is fixedly arranged at the driving end of the second motor, and the driving end of the second worm is in meshed connection with the second worm wheel.
Preferably, the middle part of the transmission belt and the multiple liquid adding mechanisms are positioned in the protective frame; the bottom of the protective frame is provided with a through groove corresponding to the transmission belt, and the transmission belt movably penetrates through the through groove; a top cover is hinged at the top end of the protective frame, and an observation window is fixedly arranged on the top cover; the top end of the top cover is fixedly provided with a handle.
Compared with the prior art, the utility model has the beneficial effects that:
1. Through setting up many liquid feeding mechanisms, realize the automatic cell culture liquid that annotates to a plurality of culture dishes, reduced experimenter's operation intensity to and adjust the position of a plurality of liquid feeding pieces according to the cultivation demand, annotate different cell culture liquid to the culture dish is automatic, promote the result of use of whole liquid feeder.
2. Through setting up transmission mechanism, be convenient for carry out automatic transmission to a plurality of culture dishes, carry out automatic filling cell culture liquid with culture dish transmission to a plurality of liquid feeding mechanism positions department in proper order.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present utility model.
FIG. 2 is a schematic diagram of a multiple liquid feeding mechanism according to the present utility model.
Fig. 3 is an enlarged view of the utility model at a in fig. 2.
Fig. 4 is a schematic structural view of the liquid adding member in the present utility model.
Fig. 5 is an enlarged view of the present utility model at B in fig. 4.
Fig. 6 is a schematic structural view of a transmission mechanism in the present utility model.
Fig. 7 is an enlarged view of the present utility model at C in fig. 6.
Fig. 8 is a schematic structural diagram of a protective frame in the present utility model.
In the figure: 1. a base; 2. a plurality of liquid adding mechanisms; 3. a transmission mechanism; 4. a culture dish; 5. a protective frame; 21. a mechanism bottom frame; 22. a rotation shaft; 23. a rotating disc; 24. a support rod; 25. a liquid adding member; 26. lifting the sleeve frame; 261. lifting the inner rod; 27. a first worm wheel; 271. a first motor; 272. a first worm; 28. a lifting rod; 251. a liquid storage cylinder; 252. a liquid discharge nozzle; 253. a cover; 254. a connecting pipe; 255. a pump machine; 256. a threaded tube; 2561. a seal ring; 257. a positioning ring; 2571. positioning columns; 31. a transmission side frame; 32. a conveying roller; 33. a conveyor belt; 34. a second worm wheel; 341. a second motor; 342. a second worm; 35. a positioning block; 51. a top cover; 501. penetrating a groove; 52. an observation window; 53. a handle.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Examples: as shown in fig. 1-8, the utility model provides a liquid feeder for cell culture, which comprises a base 1, wherein a plurality of liquid adding mechanisms 2 are fixedly arranged in the middle of the top end of the base 1, a transmission mechanism 3 is fixedly arranged at the top end of the base 1, a plurality of culture dishes 4 which are uniformly distributed are arranged on the transmission mechanism 3, and a protective frame 5 is fixedly arranged at the top end of the base 1 at the position of the plurality of liquid adding mechanisms 2;
The multiple liquid adding mechanism 2 comprises a mechanism underframe 21, the mechanism underframe 21 is fixedly arranged in the middle of the top end of the base 1, a rotating shaft 22 is arranged in the middle of the top end of the mechanism underframe 21, a rotating disc 23 is fixedly arranged at the top end of the rotating shaft 22, a plurality of support rods 24 distributed in an annular array are fixedly arranged outside the rotating disc 23, liquid adding pieces 25 are arranged at one ends of the support rods 24 far away from the rotating disc 23, different cell culture liquids are conveniently added by arranging the plurality of liquid adding pieces 25, the using effect of the whole liquid adding device is improved, a lifting sleeve frame 26 is rotatably arranged in the middle of the top end of the mechanism underframe 21, a lifting inner rod 261 is fixedly clamped at the middle of the lifting sleeve frame 26, the bottom end of the rotating shaft 22 and the top end of the lifting inner rod 261 are fixedly arranged, the lifting inner rod 261 can be lifted at the middle of the lifting sleeve frame 26 by arranging the lifting sleeve frame 26, the rotating shaft 22, the rotating disc 23, the plurality of support rods 24 and the liquid adding pieces 25 are driven to lift, and when the lifting sleeve frame 26 rotates, the rotating shaft 22, the rotating disc 23, the plurality of support rods 24 and the liquid adding pieces 25 are driven to rotate, and the positions of the liquid adding pieces 25 are flexibly changed;
The liquid adding pieces 25 comprise liquid storage cylinders 251, the liquid adding pieces 25 are movably clamped at one ends, far away from the rotating disc 23, of the corresponding support rods 24, liquid discharging nozzles 252 are fixedly arranged at the bottom ends of the liquid storage cylinders 251, sealing covers 253 are arranged at the top ends of the liquid storage cylinders 251, connecting pipes 254 are fixedly arranged in the middle of the top ends of the sealing covers 253, pump 255 are fixedly arranged at the top ends of the connecting pipes 254, different cell culture liquids are stored in the liquid storage cylinders 251 in the liquid adding pieces 25, the pump 255 is controlled to be started, air is fed into the liquid storage cylinders 251, and the cell culture liquids in the corresponding liquid storage cylinders 251 are automatically filled in the corresponding culture dishes 4 through the liquid discharging nozzles 252.
The outside fixed cover of reservoir 251 is equipped with holding ring 257, and the bottom fixed mounting of holding ring 257 has reference column 2571, and reference column 2571 activity joint is on bracing piece 24, carries out the joint between liquid feeding piece 25, the bracing piece 24, the dismantlement cleaning of being convenient for liquid feeding piece 25.
The top fixing clip of the liquid storage cylinder 251 is provided with a threaded pipe 256, the threaded pipe 256 is arranged in the sealing cover 253 in a threaded manner, a sealing ring 2561 is movably clamped in the sealing cover 253, the top end of the threaded pipe 256 is contacted with the lower surface of the sealing ring 2561 to seal the liquid storage cylinder 251 and the sealing cover 253, the sealing cover 253 is conveniently opened in a rotating manner, and the liquid storage cylinder 251 and the sealing cover 253 are detached for cleaning.
The outside bottom fixed cover of lift cover frame 26 is equipped with first worm wheel 27, mechanism underframe 21's inner wall upper surface fixed mounting has first motor 271, first worm 272 is fixed to first motor 271's driving end, first worm 272 and first worm wheel 27 meshing are connected, mechanism underframe 21's inner wall lower surface fixed mounting has lifter 28, lifter 28's drive end passes through the bearing rotation and installs in lifter 261's bottom middle part, during the use, control opens first motor 271 and drives first worm wheel 27 and rotate, thereby drive lift cover frame 26 rotates, control opens lifter 28 drive lifter 261 and go up and down.
The transmission mechanism 3 comprises two groups of symmetrically distributed transmission side frames 31, the transmission side frames 31 are fixedly arranged at the top end of the base 1, the top of each transmission side frame 31 is rotatably provided with a transmission roller 32, the outer sides of the two transmission rollers 32 are movably sleeved with transmission belts 33, the surfaces of the transmission belts 33 are fixedly provided with positioning blocks 35, the culture dishes 4 are movably clamped in the positioning blocks 35, and the lower surfaces of the culture dishes 4 are in contact with the upper surfaces of the transmission belts 33; the end of one of the transmission rollers 32 is fixedly provided with a second worm wheel 34, the outer side of the transmission side frame 31 is fixedly provided with a second motor 341, the driving end of the second motor 341 is fixedly provided with a second worm 342, the driving end of the second worm 342 is in meshed connection with the second worm wheel 34, and when the automatic cell culture medium filling device is used, the second motor 341 is controlled to be started to drive the second worm 342 to drive the second worm wheel 34 to rotate, so that one of the transmission rollers 32 is driven to rotate, the transmission belt 33 is driven to transmit, a plurality of culture dishes 4 are sequentially transmitted to the positions of the multiple liquid filling mechanisms 2 to automatically fill cell culture medium.
The middle part of the transmission belt 33 and the multiple liquid adding mechanisms 2 are positioned in the protective frame 5; top end hinge of the protection frame 5 is provided with a top cover 51, the middle part of the transmission belt 33 and the multiple liquid adding mechanisms 2 are protected through the protection frame 5 and the top cover 51, the follow-up cell culture liquid filling is prevented from being interfered by external environment, the follow-up cell culture is influenced, and an observation window 52 is fixedly arranged on the top cover 51, so that the observation of the protection frame 5 is facilitated.
A handle 53 is fixedly installed at the top end of the top cover 51, so that the handle 53 can be conveniently held and the top cover 51 can be opened by rotating.
The bottom of the protection frame 5 is provided with a through groove 501 corresponding to the transmission belt 33, the transmission belt 33 movably penetrates through the through groove 501, and the culture dish 4 outside the protection frame 5 is conveniently transmitted into the protection frame 5 through the through groove 501.
Working principle: when in use, different cell culture liquids are stored in the liquid storage cylinders 251 in the liquid adding pieces 25;
A plurality of culture dishes 4 are movably clamped in the positioning block 35 in sequence, the second motor 341 is controlled to be started to drive the second worm 342 to drive the second worm wheel 34 to rotate, so that one of the transmission rollers 32 is driven to rotate, the transmission belt 33 is driven to transmit, the culture dishes 4 are transmitted to the positions of the multiple liquid adding mechanisms 2 in sequence, and cell culture liquid is automatically added;
When the cell culture solution is filled, according to the difference of the cell culture solution, the first motor 271 is controlled to be started to drive the first worm 272 to drive the first worm wheel 27 to rotate, so that the lifting sleeve frame 26 is driven to rotate, the lifting inner rod 261, the rotating shaft 22, the rotating disc 23, the plurality of support rods 24 and the liquid adding pieces 25 are driven to rotate, the positions of the plurality of liquid adding pieces 25 are flexibly changed, the corresponding liquid adding pieces 25 are rotated to the upper part of the culture dish 4, then, the lifting rod 28 is controlled to be started to drive the lifting inner rod 261 to descend, and the liquid discharging nozzle 252 is moved into the culture dish 4;
Subsequently, the pump 255 is started to feed air into the liquid storage cylinders 251, and the cell culture liquid in the corresponding liquid storage cylinders 251 is automatically filled into the corresponding culture dishes 4 through the liquid discharge nozzles 252;
With this realization is to the automatic cell culture liquid that annotates of a plurality of culture dishes 4, has reduced the operation intensity of experimenter to and according to the position of cultivateing the demand regulation a plurality of liquid feeding pieces 25, annotate different cell culture liquid to culture dish 4 is automatic, promotes the result of use of whole liquid feeder.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A liquid filler for cell culture, comprising a base (1), characterized in that: the middle part of the top end of the base (1) is fixedly provided with a plurality of liquid adding mechanisms (2), the top end of the base (1) is fixedly provided with a transmission mechanism (3), a plurality of culture dishes (4) which are uniformly distributed are arranged on the transmission mechanism (3), and the top end of the base (1) at the position of the plurality of liquid adding mechanisms (2) is fixedly provided with a protection frame (5);
The multi-item liquid adding mechanism (2) comprises a mechanism underframe (21), the mechanism underframe (21) is fixedly arranged in the middle of the top end of the base (1), a rotating shaft (22) is arranged in the middle of the top end of the mechanism underframe (21), a rotating disc (23) is fixedly arranged at the top end of the rotating shaft (22), a plurality of support rods (24) distributed in an annular array are fixedly arranged at the outer side of the rotating disc (23), liquid adding pieces (25) are arranged at one ends, far away from the rotating disc (23), of the support rods (24), a lifting sleeve frame (26) is rotatably arranged in the middle of the top end of the mechanism underframe (21), a lifting inner rod (261) is slidably clamped in the middle of the lifting sleeve frame (26), and the bottom end of the rotating shaft (22) and the top end of the lifting inner rod (261) are fixedly arranged;
The liquid adding piece (25) comprises a liquid storage barrel (251), the liquid adding piece (25) is movably clamped at one end, far away from the rotary disc (23), of a corresponding supporting rod (24), a liquid discharging nozzle (252) is fixedly arranged at the bottom end of the liquid storage barrel (251), a sealing cover (253) is arranged at the top end of the liquid storage barrel (251), a connecting pipe (254) is fixedly arranged in the middle of the top end of the sealing cover (253), and a pump (255) is fixedly arranged at the top end of the connecting pipe (254).
2. The liquid feeder for cell culture according to claim 1, wherein: the outside fixed cover of reservoir (251) is equipped with holding ring (257), the bottom fixed mounting of holding ring (257) has reference column (2571), reference column (2571) activity joint is on bracing piece (24).
3. The liquid feeder for cell culture according to claim 1, wherein: the top fixing clamp of the liquid storage barrel (251) is provided with a threaded pipe (256), the threaded pipe (256) is installed in the sealing cover (253) in a threaded mode, the sealing cover (253) is movably clamped with a sealing ring (2561), and the top end of the threaded pipe (256) is in contact with the lower surface of the sealing ring (2561).
4. The liquid feeder for cell culture according to claim 1, wherein: the outside bottom fixed cover of lift cover frame (26) is equipped with first worm wheel (27), the inner wall upper surface fixed mounting of mechanism underframe (21) has first motor (271), the driving end fixed mounting of first motor (271) has first worm (272), first worm (272) and first worm wheel (27) meshing are connected, the inner wall lower surface fixed mounting of mechanism underframe (21) has lifter (28), the drive end of lifter (28) passes through the bottom middle part of bearing rotation installation in lifter (261).
5. The liquid feeder for cell culture according to claim 1, wherein: the conveying mechanism (3) comprises two groups of symmetrically distributed conveying side frames (31), the conveying side frames (31) are fixedly mounted at the top end of the base (1), conveying rollers (32) are rotatably mounted at the top of the conveying side frames (31), conveying belts (33) are movably sleeved on the outer sides of the conveying rollers (32), positioning blocks (35) are fixedly mounted on the surfaces of the conveying belts (33), the culture dishes (4) are movably clamped in the positioning blocks (35), and the lower surfaces of the culture dishes (4) are in contact with the upper surfaces of the conveying belts (33).
6. The liquid feeder for cell culture according to claim 5, wherein: the end part of one of the transmission rollers (32) is fixedly provided with a second worm wheel (34), the outer side of the transmission side frame (31) is fixedly provided with a second motor (341), the driving end of the second motor (341) is fixedly provided with a second worm (342), and the driving end of the second worm (342) is in meshed connection with the second worm wheel (34).
7. The liquid feeder for cell culture according to claim 5, wherein: the middle part of the transmission belt (33) and the plurality of liquid adding mechanisms (2) are positioned in the protective frame (5).
8. The liquid feeder for cell culture according to claim 5, wherein: the bottom of the protection frame (5) is provided with a through groove (501) corresponding to the transmission belt (33), and the transmission belt (33) movably penetrates through the through groove (501).
9. The liquid feeder for cell culture according to claim 1, wherein: top end hinge of the protection frame (5) is provided with a top cover (51), and an observation window (52) is fixedly arranged on the top cover (51).
10. The liquid feeder for cell culture according to claim 9, wherein: the top end of the top cover (51) is fixedly provided with a handle (53).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202420600682.9U CN220951847U (en) | 2024-03-27 | 2024-03-27 | Liquid adding device for cell culture |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202420600682.9U CN220951847U (en) | 2024-03-27 | 2024-03-27 | Liquid adding device for cell culture |
Publications (1)
Publication Number | Publication Date |
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CN220951847U true CN220951847U (en) | 2024-05-14 |
Family
ID=90975176
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202420600682.9U Active CN220951847U (en) | 2024-03-27 | 2024-03-27 | Liquid adding device for cell culture |
Country Status (1)
Country | Link |
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CN (1) | CN220951847U (en) |
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- 2024-03-27 CN CN202420600682.9U patent/CN220951847U/en active Active
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