CN220907496U - Fluorescent signal detection device of PCR instrument - Google Patents

Abstract

The utility model relates to the technical field of biological detection, in particular to a fluorescent signal detection device of a PCR instrument, which comprises a shell, wherein a closed empty bin is formed in the interior of the shell, a bin door capable of being opened or closed is arranged in the empty bin, a moving assembly, a mounting plate connected with the moving assembly, a carrier detachably arranged on the mounting plate, a detection lens arranged above the carrier, a light source assembly arranged above the detection lens and a photographing assembly are arranged in the empty bin, the moving assembly is used for driving the mounting plate to move and enter and exit the empty bin from the bin door, and the carrier is used for placing a sample container; the light source component provides excitation light, the excitation light is emitted into the sample container through the detection lens, and the sample is excited to emit fluorescence; and the photographing component photographs the sample and detects the fluorescent signal. Through business turn over storehouse design, can realize detecting multiple different grade type sample container, improve detection efficiency. The detection process is carried out in the closed shell empty bin, so that the interference of external light on the detection result is avoided.

Description

Fluorescent signal detection device of PCR instrument

Technical Field

The utility model relates to the technical field of biological detection, in particular to a fluorescent signal detection device of a PCR instrument.

Background

The polymerase chain reaction (Polymerase Chain Reaction, PCR) is a molecular biological technique for amplifying specific DNA fragments, which can be seen as specific DNA replication in vitro, the greatest feature of PCR being the ability to increase trace amounts of DNA substantially. The PCR instrument based on polymerase is actually a temperature control device, and can well control the denaturation temperature, renaturation temperature and extension temperature. The PCR detection process is usually to excite amplified samples with excitation light of different wavebands, and determine the result by detecting the intensity of the excited fluorescent signal.

In the prior art, the excitation light with different wave bands is usually generated by adopting a fluorescent turntable mode, namely, a mercury lamp provides a light source, light outside a required wave band is filtered out through a light filter, then the excitation light is reflected onto a sample through a reflecting mirror, the sample is excited, and fluorescence generated by excitation of the sample can permeate the reflecting mirror and then be collected by a camera. This approach has the following drawbacks: the sample containers of various different types cannot be detected, so that the detection efficiency is affected; multiple reflectors are needed, and the structure is complex and the cost is high; the complex structure increases the weight of the whole instrument and reduces portability; the closed environment is not ideal, and redundant external light can influence the detection result.

Disclosure of utility model

In view of the above-mentioned drawbacks of the prior art, the present utility model is to provide a fluorescence signal detection device of a PCR instrument, which can detect a plurality of different types of sample containers.

In order to solve the technical problems, the utility model adopts the following technical scheme:

The utility model provides a fluorescence signal detection device of a PCR instrument, which comprises a shell, wherein a closed empty bin is formed in the interior of the shell, a bin door capable of being opened or closed is arranged in the empty bin, a moving assembly, a mounting plate connected with the moving assembly, a carrier detachably arranged on the mounting plate, a detection lens arranged above the carrier, a light source assembly and a photographing assembly arranged above the detection lens are arranged in the empty bin, the moving assembly is used for driving the mounting plate to move and enter and exit the empty bin from the bin door, and the carrier is used for placing a sample container; the light source component provides excitation light, the excitation light is emitted into the sample container through the detection lens, and a sample in the sample container is excited to emit fluorescence; the photographing component photographs a sample in the sample container irradiated by the excitation light, and detects a fluorescent signal.

Preferably, the moving assembly comprises a first motor, a gear connected with an output shaft of the first motor and a rack meshed with the gear, the rack is connected with a mounting plate, a guide rail parallel to the rack is arranged on the shell, and the mounting plate is in sliding fit with the guide rail.

Preferably, the device further comprises a controller and a detection piece, wherein the detection piece is used for detecting whether the mounting plate moves to the position, below the detection lens, of the carrier, and the detection piece and the first motor are in communication connection with the controller.

Preferably, the light source component comprises a light emitting light source, a light transmission mirror, a first filter wheel, a reflecting mirror and a second motor for driving the first filter wheel to rotate, wherein a plurality of first optical filters are arranged on the first filter wheel along the circumferential direction, and light rays emitted by the light emitting light source are sequentially transmitted through the light transmission mirror, filtered by one first optical filter on the first filter wheel and reflected by the reflecting mirror to form excitation light and then are emitted to the detection lens.

Preferably, the light source assembly further comprises a first adjustment assembly for adjusting the position of the luminescent light source.

Preferably, the light source assembly further comprises a heat dissipation assembly for dissipating heat from the luminescent light source.

Preferably, the photographing assembly comprises a camera, an objective lens, a second filter wheel and a third motor for driving the second filter wheel to rotate, wherein a plurality of second optical filters are arranged on the second filter wheel along the circumferential direction, and fluorescence emitted by a sample in a sample container irradiated by excitation light is sequentially transmitted through the detection lens, filtered by one second optical filter on the second filter wheel and transmitted by the objective lens and then emitted to the camera.

Preferably, the photographing assembly further comprises a second adjusting assembly for adjusting the position of the camera.

Preferably, the device further comprises a double-shading barrel, the double-shading barrel is provided with two mutually separated light channels, and the excitation light provided by the light source assembly and fluorescence emitted by a sample in the sample container irradiated by the excitation light respectively pass through the two light channels of the double-shading barrel.

Preferably, the empty bin is internally provided with a supporting frame, the light source assembly and the photographing assembly are both arranged on the supporting frame, and the light source assembly and the supporting frame are both indirectly connected through the damping piece.

Compared with the prior art, the utility model has obvious progress:

According to the fluorescence signal detection device of the PCR instrument, during detection, the moving component moves the mounting plate to the position of the carrier right below the detection lens so as to detect at the detection station, before detection and after detection are completed, the bin gate can be opened, the driving component drives the mounting plate to drive the carrier to move and move out of the empty bin of the shell from the bin gate, so that sample containers can be placed or replaced on the carrier outside the shell, and various different types of sample containers such as single tubes, 8-connected tubes, 96-well plates (without skirt edges, half skirt edges and full skirt edges) can be used for compatible detection; meanwhile, the carrier can be disassembled and replaced, and when a special sample container is met according to the design and manufacture of the special sample container, for example, a special microfluidic chip is needed to be replaced, so that the detection can be easily performed, and the cost waste caused by replacing a large number of mould changing pieces can be saved. After the carrier or the sample container is replaced outside the shell, the driving assembly drives the mounting plate to drive the carrier to move, the carrier enters the shell empty bin from the bin gate and returns to the detection station, the carrier is positioned right below the detection lens, and the bin gate is closed, so that the next detection can be performed. Therefore, the fluorescence signal detection device of the PCR instrument can realize detection of various sample containers with different types through the design of the in-out bin, and improves the detection efficiency. Meanwhile, according to the fluorescence signal detection device of the PCR instrument, the fluorescence signal detection process is carried out in the closed shell empty bin, so that the interference of external light on a detection result is avoided.

Drawings

FIG. 1 is a schematic view showing the appearance of a fluorescence signal detecting apparatus of a PCR instrument according to an embodiment of the present utility model.

FIG. 2 is a schematic diagram showing a structure of a fluorescence signal detecting apparatus of a PCR instrument according to an embodiment of the present utility model with a housing removed.

FIG. 3 is a schematic longitudinal section of a fluorescence signal detecting apparatus of a PCR instrument according to an embodiment of the present utility model with a housing removed.

FIG. 4 is a schematic diagram showing the structure of a moving assembly in a fluorescence signal detecting apparatus of a PCR instrument according to an embodiment of the present utility model.

Fig. 5 is a schematic structural diagram of a light source assembly in a fluorescent signal detection device of a PCR instrument according to an embodiment of the present utility model.

Fig. 6 is an exploded view of a light source assembly in a fluorescent signal detection device of a PCR instrument according to an embodiment of the present utility model.

Fig. 7 is a schematic structural diagram of a photographing assembly in the fluorescent signal detection device of the PCR instrument according to the embodiment of the present utility model.

Fig. 8 is an exploded view of a photographing assembly in a fluorescent signal detecting device of a PCR instrument according to an embodiment of the present utility model.

Fig. 9 is a schematic diagram of a limiting structure of a second cover plate of a photographing assembly to an objective lens in a fluorescent signal detection device of a PCR instrument according to an embodiment of the present utility model.

Wherein reference numerals are as follows:

1. First coupling of shell 6-16

1-1 Bin gate 6-17 first photoelectric switch

1-2 Casing 6-18 lens press ring

1-3 Base plate 6-19 lens mounting block

2. Moving assembly 6-20 connecting block

2-1 First motor 6-21 connecting plate

2-2 Gear 6-22 silica gel pad

2-3 Rack 7 shooting assembly

2-4 Guide rail 7-1 camera

3. Mounting plate 7-2 objective lens

4. Carrier 7-3 second filter wheel

4-1 Positioning column 7-4 third motor

5. Second filter of detection lens 7-5

5-1 Lens holder 7-6 second adjusting Assembly

6. Light source assembly 7-7 support plate

6-1 Luminous light source 7-8 second fixing disk

6-2 Light-transmitting lens 7-8a second light-transmitting hole

6-3 First filter wheel 7-9 second cover plate

6-4 Mirror 7-9a cover plate hole

6-5 Second motor 7-10 second compression ring

6-6 First optical filter 7-11 second rotation shaft

6-7 First adjusting component 7-12 motor mounting plate

6-8 Radiator 7-13 second coupling

6-9 Fan 7-14 second photoelectric switch

6-10 Fixed seat 7-15 baffle

6-11 Motor fixing plate 7-16 slipway mounting plate

6-12 First fixed disk 7-17 camera mounting seat

6-12A first light hole 8 double-shading barrel

6-13 First cover plate 8a optical channel

6-14 First pressure ring 9 controller

6-15 First rotating shaft 10 supporting frame

Detailed Description

The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present utility model and are not intended to be limiting.

In the description of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

As shown in fig. 1 to 9, one embodiment of a fluorescence signal detecting apparatus of a PCR instrument of the present utility model.

Referring to fig. 1, 2 and 3, the fluorescence signal detecting device of the PCR instrument of the present embodiment includes a housing 1, wherein a closed empty chamber is formed by the interior of the housing 1, and the empty chamber is provided with a door 1-1 that can be opened or closed. Preferably, the shell 1 comprises a shell 1-2 and a bottom plate 1-3, wherein a cavity with a closed upper end and an open lower end is formed in the shell 1-2, the bottom plate 1-3 is connected with the lower end of the shell 1-2 and seals the opening of the cavity, so that a closed empty bin is formed, and the bin door 1-1 is arranged on the shell 1-2.

The empty bin of the shell 1 is internally provided with a moving component 2, a mounting plate 3, a carrier 4, a detection lens 5, a light source component 6 and a photographing component 7. The mounting plate 3 is connected with the moving assembly 2, and the moving assembly 2 is used for driving the mounting plate 3 to move and enabling the mounting plate 3 to enter and leave the shell 1 from the bin gate 1-1 to be empty. The carrier 4 is used for placing a sample container, and the sample container is filled with a sample to be detected. The carriage 4 is detachably provided on the mounting plate 3 so as to move synchronously with the mounting plate 3. The detection lens 5 is arranged above the carrier 4, and the light source assembly 6 and the photographing assembly 7 are arranged above the detection lens 5. During detection, the moving assembly 2 moves the mounting plate 3 to a position where the carrier 4 is located right below the detection lens 5, which is a detection station. The light source assembly 6 provides excitation light which is emitted into the sample container on the carrier 4 of the detection station through the detection lens 5, and excites the sample in the sample container to emit fluorescence. The photographing assembly 7 photographs a sample in the sample container irradiated with the excitation light, and detects a fluorescent signal. Before and after detection, the bin gate 1-1 can be opened, the driving assembly 2 drives the mounting plate 3 to drive the carrier 4 to move and the bin gate 1-1 to move out of the empty bin of the shell 1, so that sample containers can be placed or replaced on the carrier 4 outside the shell 1, and various different types of sample containers such as single tubes, 8-connected tubes, 96-well plates (without skirt, half skirt, full skirt) and the like can be used for compatible detection; meanwhile, the carrier 4 can be disassembled and assembled for replacement, and when a special sample container is met, such as a special microfluidic chip, the carrier 4 is replaced, so that the detection can be easily performed, and the cost waste caused by replacing a large number of mold changing pieces can be saved. After the carrier 4 or the sample container is replaced outside the shell 1, the driving component 2 drives the mounting plate 3 to drive the carrier 4 to move, the carrier 4 enters the empty bin of the shell 1 from the bin gate 1-1 and returns to the detection station, the carrier 4 is positioned right below the detection lens 5, and the bin gate 1-1 is closed, so that the next detection can be performed. Therefore, the fluorescence signal detection device of the PCR instrument can realize detection of various sample containers with different types through the design of the in-out bin, and the detection efficiency is improved. Meanwhile, in the fluorescence signal detection device of the PCR instrument, the fluorescence signal detection process is carried out in the empty bin of the closed shell 1, so that the interference of external light on the detection result is avoided.

In this embodiment, preferably, the moving assembly 2 is disposed on the bottom plate 1-3 of the housing 1, the carrier 4 is detachably disposed on the upper surface of the mounting plate 3, the carrier 4 is connected with the mounting plate 3 through the positioning column 4-1 to limit, and the detection lens 5 is supported on the bottom plate 1-3 through the lens bracket 5-1 and is erected above the carrier 4. A supporting frame 10 is arranged in the empty bin of the shell 1, the supporting frame 10 is erected on the bottom plate 1-3, and the light source component 6 and the photographing component 7 are both arranged on the supporting frame 10 and erected above the detection lens 5.

Referring to fig. 4, in the present embodiment, preferably, the moving assembly 2 includes a first motor 2-1, a gear 2-2 connected to an output shaft of the first motor 2-1, and a rack 2-3 engaged with the gear 2-2, the rack 2-3 is connected to a mounting plate 3, a guide rail 2-4 disposed parallel to the rack 2-3 is provided on the housing 1, and the mounting plate 3 is slidably engaged with the guide rail 2-4. The guide rail 2-4 and the first motor 2-1 are arranged on the bottom plate 1-3 of the shell 1, and the guide rail 2-4 extends from the detection station to the position of the bin gate 1-1. The output shaft of the first motor 2-1 rotates to drive the gear 2-2 to rotate, the rack 2-3 is driven to do linear motion, the rack 2-3 drives the mounting plate 3 to slide along the guide rail 2-4, and therefore the carrier 4 can be driven to move along the guide rail 2-4, and the bin door 1-1 enters and exits the shell 1 for empty bin.

Further, the fluorescence signal detecting apparatus of this embodiment further includes a controller 9 and a detecting member (not shown in the drawings), the controller 9 may be mounted on the support frame 10, and the detecting member may be mounted on the bottom plate 1-3 of the housing 1. The detecting piece is used for detecting whether the mounting plate 3 moves to the position, below the detection lens 5, of the carrier 4, namely, whether the mounting plate 3 and the carrier 4 reach a detection station, and the detecting piece and the first motor 2-1 are in communication connection with the controller 9. In the process that the first motor 2-1 drives the carrier 4 to move into the empty bin of the shell 1, when the detecting piece detects that the mounting plate 3 moves to the position, below the detecting lens 5, of the carrier 4, a detecting signal of the detecting piece is transmitted to the controller 9, the controller 9 receives the detecting signal and controls the first motor 2-1 to stop working according to the detecting signal, so that the mounting plate 3 and the carrier 4 stay at a detecting station, and at the moment, the carrier 4 is located right below the detecting lens 5. The form of the controller 9 is not limited, and an existing conventional controller such as a PLC controller or a single chip microcomputer may be used. The detection member may preferably be a photoelectric switch.

Referring to fig. 5 and 6, in the present embodiment, the light source assembly 6 preferably includes a light emitting light source 6-1, a light transmitting mirror 6-2, a first filter wheel 6-3, a reflecting mirror 6-4, and a second motor 6-5 for driving the first filter wheel 6-3 to rotate. The first filter wheel 6-3 is provided with a plurality of first optical filters 6-6 along the circumferential direction, and the plurality of first optical filters 6-6 are uniformly arranged on the first filter wheel 6-3 along the circumferential direction of the first filter wheel 6-3. The light-emitting source 6-1 is preferably an LED light source. The light-transmitting lens 6-2, the first filter wheel 6-3 and the reflecting mirror 6-4 are sequentially arranged on the light path of the light emitted by the light-emitting source 6-1, a plurality of first optical filters 6-6 on the first filter wheel 6-3 have different filtering performances, and the second motor 6-5 drives the first filter wheel 6-3 to rotate, so that one first optical filter 6-6 appointed to be needed on the first filter wheel 6-3 is positioned on the light path of the light-emitting source 6-1, and the light emitted by the light-emitting source 6-1 is correspondingly filtered. The light emitted by the light source 6-1 is transmitted through the light-transmitting mirror 6-2 in sequence, a first optical filter 6-6 on the first optical filter wheel 6-3 filters unnecessary wave bands and reflects off the reflecting mirror 6-4 to form excitation light, the excitation light is emitted to the detection lens 5, and then is transmitted and focused through the detection lens 5 to be emitted into a sample container on the carrier 4, so that a sample in the sample container is excited to emit fluorescence.

Preferably, the light source assembly 6 further comprises a first adjusting assembly 6-7 for adjusting the position of the light emitting source 6-1, and if the light emitting source 6-1 is offset during the light source testing process, the position of the light emitting source 6-1 can be finely adjusted by the first adjusting assembly 6-7 to be adjusted to a proper position. The first adjusting component 6-7 is preferably a first sliding table, the first sliding table is an existing standard component with translation and lifting functions, one side of the first sliding table is fixed, the other side of the first sliding table is connected with the luminous light source 6-1, and the position of the luminous light source 6-1 can be adjusted by manually adjusting the first sliding table.

Preferably, the light source assembly 6 further comprises a heat dissipation assembly for dissipating heat of the light emitting light source 6-1. The heat radiation component comprises a heat radiator 6-8 and a fan 6-9, the luminous light source 6-1 is arranged on one side of the heat radiator 6-8, and the fan 6-9 is arranged on the other side of the heat radiator 6-8. The radiating component is used for radiating the luminous light source 6-1, so that the reduction of the service life of elements caused by the overhigh temperature of the luminous light source 6-1 in the long-term use process can be prevented.

In this embodiment, the light source assembly 6 is mounted on the support frame 10 through the fixing base 6-10 and the motor fixing plate 6-11. Specifically, the lower end of the fixed seat 6-10 is supported on the supporting frame 10, the upper end of the fixed seat 6-10 is fixedly provided with a first fixed disk 6-12, and the first fixed disk 6-12 is in butt joint with the first cover plate 6-13. The plurality of first optical filters 6-6 are respectively and fixedly arranged on the first filter wheel 6-3 through the first compression ring 6-14, a first rotating shaft 6-15 is fixedly arranged in the center of the first filter wheel 6-3 in a penetrating mode, the first filter wheel 6-3 is packaged between the first fixed disc 6-12 and the first cover plate 6-13, and two ends of the first rotating shaft 6-15 respectively penetrate out of the first fixed disc 6-12 and the first cover plate 6-13 in a rotatable mode. The first fixing plate 6-12 is provided with a first light hole 6-12a, and the first light hole 6-12a is positioned on the light path of the luminous light source 6-1 and used for allowing light rays emitted by the luminous light source 6-1 to pass through. In the process of rotating the first filter wheel 6-3 for one circle, each first filter 6-6 on the first filter wheel 6-3 sequentially passes through the first light holes 6-12a. The lower end of the motor fixing plate 6-11 is supported on the supporting frame 10, the second motor 6-5 is fixedly arranged at the upper end of the motor fixing plate 6-11, an output shaft of the second motor 6-5 is connected with one end of the first rotating shaft 6-13 penetrating out of the first fixing disc 6-12 through the first coupler 6-16, and the first rotating shaft 6-13 can be driven to rotate, so that the first filter wheel 6-3 is driven to rotate. The first cover plate 6-13 is provided with a first photoelectric switch 6-17, and the first photoelectric switch 6-17 and one end of the first rotating shaft 6-13 penetrating out of the first cover plate 6-13 are arranged oppositely and used for detecting the angle position of the first rotating shaft 6-13. The first photoelectric switch 6-17 and the second motor 6-5 are both in communication connection with the controller 9, the first photoelectric switch 6-17 transmits the detected angle position information of the first rotating shaft 6-13 to the controller 9, and the controller 9 receives and controls the rotation angle of the second motor 6-5 according to the information, so that the rotation angles of the first rotating shaft 6-13 and the first filter wheel 6-3 are controlled, and the first filter 6-6 required by the specification is positioned at the first light transmission hole 6-12a and positioned on the light path of the luminous light source 6-1. The light-transmitting mirror 6-2 is fixedly arranged on the lens mounting block 6-19 through the lens pressing ring 6-18, the lens mounting block 6-19 is positioned on the outer side of the first fixed disk 6-12, and the light-transmitting mirror 6-2 is coaxial with the first light-transmitting hole 6-12a. The radiator 6-8 and the fan 6-9 are fixed on the connecting block 6-20, the connecting block 6-20 is fixedly connected with the lens mounting block 6-19 and is positioned on one side of the lens mounting block 6-19 far away from the first fixed disk 6-12, so that one side surface of the radiator 6-8 embedded with the luminous light source 6-1 is attached to the lens mounting block 6-19, and the luminous light source 6-1 is arranged towards the transparent mirror 6-2. Thus, the light-emitting source 6-1 and the light-transmitting mirror 6-2 are fixedly disposed with respect to each other. One side of the first adjusting component 6-7 (the first sliding table) is fixedly arranged on the first fixed disc 6-12, the other side of the first adjusting component 6-7 (the first sliding table) is connected with the lens mounting block 6-19 through the connecting plate 6-21, when the first adjusting component 6-7 (the first sliding table) is adjusted, the lens mounting block 6-19 is driven to move along with the movement of the first adjusting component 6-7, so that the lens 6-2, the connecting block 6-20, the radiator 6-8, the fan 6-9 and the luminous light source 6-1 are driven to synchronously move, the position of the luminous light source 6-1 is adjusted, and the position of the lens 6-2 is adjusted along with the movement of the first adjusting component. The light emitted from the light source 6-1 is transmitted through the light-transmitting mirror 6-2, filtered by the first light-transmitting hole 6-12a and a first optical filter 6-6 positioned at the first light-transmitting hole 6-12a, and then is emitted to the reflecting mirror 6-4. The reflecting mirror 6-4 is mounted on the first cover plate 6-13 and is disposed opposite to the first light transmitting hole 6-12a, and the excitation light reflected by the reflecting mirror 6-4 is emitted downward to the detection lens 5. The reflecting mirror 6-4 can be fastened to the first cover plate 6-13 by means of a silica gel pad 6-22 and screw press.

Referring to fig. 7, 8 and 9, in the present embodiment, it is preferable that the photographing assembly 7 includes a camera 7-1, an objective lens 7-2, a second filter wheel 7-3, and a third motor 7-4 driving the second filter wheel 7-3 to rotate. A plurality of second optical filters 7-5 are circumferentially arranged on the second filter wheel 7-3, and the plurality of second optical filters 7-5 are uniformly arranged on the second filter wheel 7-3 along the circumferential direction of the second filter wheel 7-3. The detection lens 5, the second filter wheel 7-3 and the objective lens 7-2 are sequentially arranged on a fluorescent light path emitted by a sample in a sample container irradiated by excitation light, a plurality of second optical filters 7-5 on the second filter wheel 7-3 have different filtering performances, and the third motor 7-4 drives the second filter wheel 7-3 to rotate, so that one second optical filter 7-5 appointed to be needed on the second filter wheel 7-3 is positioned on the fluorescent light path emitted by the sample, and fluorescence emitted by the sample is correspondingly filtered. Fluorescent light emitted by a sample in the sample container irradiated by the excitation light is sequentially transmitted through the detection lens 5, filtered by one second optical filter 7-5 on the second filter wheel 7-3 and transmitted by the objective lens 7-2 and then emitted to the camera 7-1, so that the camera 7-1 photographs the sample in the sample container irradiated by the excitation light to acquire fluorescent signals.

Preferably, the photographing assembly 7 further comprises a second adjusting assembly 7-6 for adjusting the position of the camera 7-1, and if the camera 7-1 is offset during photographing, the position of the camera 7-1 can be finely adjusted by the second adjusting assembly 7-6 to be adjusted to a proper position. The second adjusting component 7-6 is preferably a second sliding table, the second sliding table is an existing standard component with translation and lifting functions, one side of the second sliding table is fixed, the other side of the second sliding table is connected with the camera 7-1, and the position of the camera 7-1 can be adjusted by manually adjusting the second sliding table.

The fluorescence signal detection device of the PCR instrument of the embodiment performs fine adjustment on the position of the light-emitting source 6-1 by arranging the first adjusting component 6-7, and performs fine adjustment on the position of the camera 7-1 by arranging the second adjusting component 7-6, so that the light source test and the offset adjustment of the light-emitting source 6-1 and the camera 7-1 in the photographing process can be realized, the positions of the light-emitting source 6-1 and the camera 7-1 are adjusted to proper positions, thereby ensuring the detection effect, and simultaneously solving the problems of photographing and unadjustable light source caused by part manufacturing errors and component assembly errors of the light-emitting source 6-1 and the camera 7-1.

For convenience of operation, an operation door (not shown in the figure) for an operator to manually operate the first adjusting component 6-7 (the first sliding table) and the second adjusting component 7-6 (the second sliding table) by extending the operator into the casing 1 may be provided on the casing 1-2, and the operation door may be opened or closed, and when the adjustment is required, the operation door is opened to facilitate the operation of the first adjusting component 6-7 (the first sliding table) and the second adjusting component 7-6 (the second sliding table), and when the adjustment is completed, the operation door is closed to ensure the tightness of the empty chamber of the casing 1.

In this embodiment, the photographing assembly 7 is mounted on the support frame 10 through the support plate 7-7. Specifically, two support plates 7-7 are arranged, the two support plates 7-7 are oppositely arranged at intervals, the lower ends of the two support plates 7-7 are supported on the support frame 10, a second fixing disc 7-8 is fixedly installed and supported at the upper ends of the two support plates 7-7, and the second fixing disc 7-8 is in butt joint with a second cover plate 7-9. The plurality of second optical filters 7-5 are respectively and fixedly arranged on the second filter wheel 7-3 through a second compression ring 7-10, a second rotating shaft 7-11 is fixedly arranged in the center of the second filter wheel 7-3 in a penetrating mode, the second filter wheel 7-3 is packaged between the second fixed disc 7-8 and the second cover plate 7-9, and two ends of the second filter wheel 7-3 respectively penetrate out of the second fixed disc 7-8 and the second cover plate 7-9 in a rotatable mode. The second fixing plate 7-8 is provided with a second light hole 7-8a, and the second light hole 7-8a is positioned on a fluorescent light path emitted by the sample and used for passing through fluorescence. In the process of rotating the second filter wheel 7-3 for one circle, each second filter 7-5 on the second filter wheel 7-3 sequentially passes through the second light holes 7-8a. The third motor 7-4 is mounted and fixed on the two support plates 7-7 by motor mounting plates 7-12 and is located between the two support plates 7-7. The output shaft of the third motor 7-4 is connected with one end of the second rotating shaft 7-11 penetrating out of the second fixed disc 7-8 through the second coupler 7-13, and can drive the second rotating shaft 7-11 to rotate, so that the second filter wheel 7-3 is driven to rotate. The second cover plate 7-9 is provided with a second photoelectric switch 7-14, and the second photoelectric switch 7-14 and one end of the second rotating shaft 7-11 penetrating out of the second cover plate 7-9 are arranged opposite to each other and used for detecting the angle position of the second rotating shaft 7-11. The second photoelectric switch 7-14 and the third motor 7-4 are both in communication connection with the controller 9, the second photoelectric switch 7-14 transmits the detected angle position information of the second rotating shaft 7-11 to the controller 9, and the controller 9 receives and controls the rotation angle of the third motor 7-4 according to the information, so that the rotation angles of the second rotating shaft 7-11 and the second filter wheel 7-3 are controlled, and one second filter 7-5 required by the specification is positioned at the second light transmission hole 7-8a and positioned on a fluorescent light path emitted by a sample. The second cover plate 7-9 is provided with a through cover plate hole 7-9a, and the cover plate hole 7-9a is coaxial with the second light transmission hole 7-8a. The lower end of the objective lens 7-2 is inserted into the cover plate hole 7-9a. The lower surface of the second cover plate 7-9 is provided with a baffle plate 7-15 extending to the lower part of the cover plate hole 7-9a and used for limiting the contact limit of the end face of the lower end of the objective lens 7-2 so as to limit the lowest position of the lower end of the objective lens 7-2, which can be inserted into the cover plate hole 7-9a, and the lowest position ensures the minimum distance between the objective lens 7-2 and the second filter wheel 7-3 and prevents the objective lens 7-2 from colliding with the second filter wheel 7-3. The camera 7-1 is fixedly connected with the upper end of the objective lens 7-2. One side of a second adjusting component 7-6 (a second sliding table) is fixed on a sliding table mounting plate 7-16, the sliding table mounting plate 7-16 is fixedly supported on a second cover plate 7-9, the other side of the second adjusting component 7-6 (the second sliding table) is connected with a camera 7-1 through a camera mounting seat 7-17, when the second adjusting component 7-6 (the second sliding table) is adjusted, the camera mounting seat 7-17 is driven to move along with the second adjusting component through the movement of the second adjusting component 7-6, so that the camera 7-1 and an objective lens 7-2 are driven to synchronously move along with the second adjusting component, the position of the camera 7-1 is adjusted, and the position of the objective lens 7-2 is adjusted along with the camera. The fluorescence emitted by the sample in the sample container irradiated by the excitation light is emitted upwards to the measuring lens 5, is transmitted by the detecting lens 5, is filtered by the second light-transmitting hole 7-8a and the second light-filtering piece 7-5 positioned at the second light-transmitting hole 7-8a, then is emitted to the lower end of the objective lens 7-2 positioned at the cover plate hole 7-9a, is transmitted by the objective lens 7-2, and is emitted to the camera 7-1.

In order to avoid interference of the excitation light of the light source assembly 6 with the photographing result of the camera 7-1, preferably, the fluorescence signal detecting device of the PCR instrument of the present embodiment further includes a double-shielding barrel 8, the double-shielding barrel 8 has two light channels 8a spaced apart from each other, and the excitation light provided by the light source assembly 6 and the fluorescence emitted from the sample in the sample container irradiated with the excitation light respectively pass through the two light channels 8a of the double-shielding barrel 8. In this embodiment, the double-shading barrel 8 is connected to the lower side of the first cover plate 6-13 of the light source assembly 6 and the lower side of the second fixed disk 7-8 of the photographing assembly 7 at the same time, so that one light channel 8a of the double-shading barrel 8 is opposite to the reflecting mirror 6-4 of the light source assembly 6, and the excitation light reflected by the reflecting mirror 6-4 is emitted downwards to the detection lens 5 through one light channel 8a of the double-shading barrel 8; the other light channel 8a of the double-shading barrel 8 is opposite to the second light-transmitting hole 7-8a on the second fixed disk 7-8 of the photographing component 7, and the fluorescence transmitted by the detection lens 5 is emitted to the second optical filter 7-5 at the second light-transmitting hole 7-8a through the other light channel 8a of the double-shading barrel 8. Therefore, the excitation light reflected by the reflecting mirror 6-4 and the photographing channel of the camera 7-1 are separated by the double-shading barrel 8, and the influence of the light emitted by the luminous light source 6-1 on the photographing result can be effectively avoided.

In summary, in the fluorescence signal detection device of the PCR instrument of the present embodiment, only one reflecting mirror 6-4 is used for the light source assembly 6 and the photographing assembly 7, so that the production cost is saved, and the structure is more compact and simplified.

In this embodiment, in order to ensure the tightness of the light path, in the light source assembly 6, the heat radiator 6-8 is tightly connected with the lens mounting block 6-19, the lens mounting block 6-19 is tightly connected with the first fixed disk 6-12, the first cover plate 6-13 is tightly connected with the first fixed disk 6-12, the reflecting mirror 6-4 is tightly connected with the first cover plate 6-13, and the lower side of the first cover plate 6-13 is tightly connected with the double-shading cylinder 8 so as to form a sealed excitation light path; in the photographing assembly 7, a gap between the outer peripheral surface of the objective lens 7-2 and the cover plate hole 7-9a is filled and sealed by using a flexible material (such as a silica gel pad), and a sealed fluorescent path is formed between the lower side of the second fixing disc 7-8 and the double-shading cylinder 8, between the second fixing disc 7-8 and the second cover plate 7-9, and between the camera 7-1 and the objective lens 7-2. Therefore, the interference of external light on the detection result can be avoided.

In this embodiment, the light source assembly 6 and the support frame 10, and the photographing assembly 7 and the support frame 10 are preferably indirectly connected through shock absorbing members. Specifically, shock absorbing members are arranged between the fixing seat 6-10 of the light source assembly 6 and the supporting frame 10, and between the motor fixing plate 6-11 and the supporting frame 10, and shock absorbing members are arranged between the two supporting plates 7-7 of the photographing assembly 7 and the supporting frame 10. Therefore, the light source assembly 6 and the photographing assembly 7 are not directly connected with the supporting frame 10, when the shell 1 or the supporting frame 10 shakes or the external environment shakes, the light source assembly 6 and the photographing assembly 7 cannot generate obvious shake to influence fluorescence detection through the damping and buffering effects of the damping piece, and the fluorescence detection effect can be more reliable. The form of the shock absorbing member is not limited, and for example, a conventional shock absorber or a soft rubber pad may be used.

The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.

Claims (10)

1. The fluorescent signal detection device of the PCR instrument is characterized by comprising a shell (1), wherein a closed empty bin is formed in the interior of the shell (1), an openable or closable bin door (1-1) is arranged in the empty bin, a moving assembly (2), a mounting plate (3) connected with the moving assembly (2), a carrier (4) detachably arranged on the mounting plate (3), a detection lens (5) arranged above the carrier (4), a light source assembly (6) arranged above the detection lens (5) and a photographing assembly (7) are arranged in the empty bin, the moving assembly (2) is used for driving the mounting plate (3) to move and enter and exit the empty bin from the bin door (1-1), and the carrier (4) is used for placing a sample container; the light source assembly (6) provides excitation light, the excitation light is emitted into the sample container through the detection lens (5), and a sample in the sample container is excited to emit fluorescence; the photographing component (7) photographs a sample in the sample container irradiated by the excitation light, and detects a fluorescent signal.

2. The fluorescence signal detection device of the PCR instrument according to claim 1, wherein the moving assembly (2) comprises a first motor (2-1), a gear (2-2) connected with an output shaft of the first motor (2-1) and a rack (2-3) meshed with the gear (2-2), the rack (2-3) is connected with the mounting plate (3), a guide rail (2-4) parallel to the rack (2-3) is arranged on the housing (1), and the mounting plate (3) is in sliding fit with the guide rail (2-4).

3. The fluorescence signal detection device of a PCR instrument according to claim 2, further comprising a controller (9) and a detection member for detecting whether the mounting plate (3) is moved to the position where the carrier (4) is located below the detection lens (5), the detection member and the first motor (2-1) being both in communication with the controller (9).

4. The fluorescence signal detection device of the PCR instrument according to claim 1, wherein the light source assembly (6) includes a light source (6-1), a light-transmitting mirror (6-2), a first filter wheel (6-3), a reflecting mirror (6-4) and a second motor (6-5) for driving the first filter wheel (6-3) to rotate, a plurality of first optical filters (6-6) are circumferentially arranged on the first filter wheel (6-3), and light emitted by the light source (6-1) is sequentially transmitted through the light-transmitting mirror (6-2), filtered by one of the first optical filters (6-6) on the first filter wheel (6-3), reflected by the reflecting mirror (6-4), and forms the excitation light and is emitted to the detection lens (5).

5. The fluorescence signal detection device of a PCR instrument according to claim 4, wherein the light source assembly (6) further comprises a first adjustment assembly (6-7) for adjusting the position of the light emitting light source (6-1).

6. The fluorescence signal detection device of a PCR instrument according to claim 4, wherein the light source assembly (6) further comprises a heat sink assembly for dissipating heat from the light emitting light source (6-1).

7. The fluorescence signal detection device of the PCR instrument according to claim 1, wherein the photographing assembly (7) includes a camera (7-1), an objective lens (7-2), a second filter wheel (7-3) and a third motor (7-4) for driving the second filter wheel (7-3) to rotate, a plurality of second filters (7-5) are circumferentially arranged on the second filter wheel (7-3), and fluorescence emitted by a sample in a sample container irradiated with excitation light is sequentially transmitted through the detection lens (5), filtered by one second filter (7-5) on the second filter wheel (7-3) and transmitted by the objective lens (7-2) and then is emitted to the camera (7-1).

8. The fluorescence signal detection device of a PCR instrument according to claim 7, wherein the photographing assembly (7) further comprises a second adjustment assembly (7-6) for adjusting the position of the camera (7-1).

9. The fluorescence signal detection device of a PCR instrument according to claim 1, further comprising a double light shielding tube (8), wherein the double light shielding tube (8) has two light channels (8 a) spaced apart from each other, and the excitation light provided by the light source assembly (6) and the fluorescence emitted from the sample in the sample container irradiated with the excitation light pass through the two light channels (8 a) of the double light shielding tube (8), respectively.

10. The fluorescence signal detection device of the PCR instrument according to claim 1, wherein a supporting frame (10) is disposed in the empty bin, the light source assembly (6) and the photographing assembly (7) are both disposed on the supporting frame (10), and the light source assembly (6) and the supporting frame (10) and the photographing assembly (7) and the supporting frame (10) are both indirectly connected through a shock absorbing member.