CN220860715U - Device for maintaining liquid chromatographic column - Google Patents
Device for maintaining liquid chromatographic column Download PDFInfo
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- CN220860715U CN220860715U CN202322779701.7U CN202322779701U CN220860715U CN 220860715 U CN220860715 U CN 220860715U CN 202322779701 U CN202322779701 U CN 202322779701U CN 220860715 U CN220860715 U CN 220860715U
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- 239000007788 liquid Substances 0.000 title claims abstract description 66
- 238000001802 infusion Methods 0.000 claims abstract description 29
- 238000012423 maintenance Methods 0.000 claims abstract description 26
- 239000002699 waste material Substances 0.000 claims abstract description 22
- 239000002904 solvent Substances 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 238000004811 liquid chromatography Methods 0.000 claims description 7
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000012071 phase Substances 0.000 claims 7
- 239000007791 liquid phase Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 18
- 239000000306 component Substances 0.000 description 6
- 238000013461 design Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model belongs to the technical field of maintenance of chromatographic columns in laboratories, and discloses a device for maintenance of a liquid chromatographic column, which comprises a mobile phase placing box, wherein four mobile phase solvent bottles are placed in the mobile phase placing box, an infusion pump box is arranged outside the mobile phase placing box, a single pump and a quaternary proportional valve are fixed in the infusion pump box, and the quaternary proportional valve is connected with the four mobile phase solvent bottles; the outer side of the infusion pump box is provided with a column temperature box and a waste liquid collecting bottle, the column temperature box is connected with the liquid outlet end of the single pump through a second infusion tube, a heating component and a chromatographic column mounting frame are arranged in the column temperature box, and a temperature sensor is arranged on the column temperature box; the waste liquid collecting bottle is connected with the column incubator; the waste liquid pipe is connected with a joint; the quaternary proportional valve is also electrically connected with a controller, and the controller is also electrically connected with the single pump, the heating component and the temperature sensor; the utility model solves the problem that the maintenance of the chromatographic column is inconvenient under the condition of the operation load of laboratory detection equipment, and is suitable for the maintenance of the liquid chromatographic column.
Description
Technical Field
The utility model relates to the technical field of maintenance of chromatographic columns in laboratories, in particular to a device for maintenance of a liquid chromatographic column.
Background
In quantitative analysis of a laboratory liquid chromatograph, a liquid chromatograph tandem mass spectrometer and an ion chromatograph, a chromatographic column is used as one of core components of the liquid chromatograph, and the separation degree and the stability of the chromatographic column are critical to the accuracy of quantitative analysis results, so that the periodic maintenance of the liquid chromatographic column is extremely important. The maintenance of the chromatographic column mainly comprises activation before starting a new chromatographic column, flushing after use and full balance before use, at present, the maintenance of the chromatographic column under three conditions in a conventional laboratory is completed under a chromatographic detection system, the time of occupying equipment for each maintenance ring section is up to several hours for effectively maintaining the good working state of the liquid chromatographic column, and the maintenance of the chromatographic column occupies more time and reduces the detection working efficiency for the inspection mechanism with limited equipment quantity, abundant detection indexes and large detection workload. Meanwhile, the maintenance of the liquid chromatographic column is finished by adopting a chromatographic detection system, and the pollution to a detection equipment pipeline is aggravated by impurities eluted by the chromatographic column, so that the maintenance device for the liquid chromatographic column is necessary to design, the maintenance work of the chromatographic column is separated from analysis equipment, the scientific maintenance of the chromatographic column can be ensured under the condition of the operation load of laboratory detection equipment, the service performance of the chromatographic column is ensured, and the service life of the chromatographic column is effectively prolonged.
Disclosure of utility model
The present utility model is directed to a device for maintaining a liquid chromatography column, which solves the above-mentioned problems in the prior art.
In order to achieve the above object, the present utility model provides the following technical solutions:
The basic technical scheme provided by the utility model is as follows: the device comprises a mobile phase placing box, wherein four mobile phase solvent bottles are placed in the mobile phase placing box, an infusion pump box is arranged on the outer side of the mobile phase placing box, a single pump and a quaternary proportional valve are fixed in the infusion pump box, the quaternary proportional valve comprises four liquid inlet ports and one liquid outlet port, and the four liquid inlet ports are connected with the four mobile phase solvent bottles in a one-to-one correspondence manner through a first infusion tube; the liquid inlet end of the single pump is connected with the liquid outlet port;
The outside of the infusion pump box is provided with a column temperature box and a waste liquid collecting bottle, the column temperature box is connected with the liquid outlet end of the single pump through a second infusion tube, a heating component and a chromatographic column mounting rack are further arranged in the column temperature box, and a temperature sensor for monitoring the temperature inside the column temperature box is further arranged on the column temperature box; the waste liquid collecting bottle is connected with the column incubator through a waste liquid pipe; the liquid inlet end of the waste liquid pipe and the liquid outlet end of the second infusion pipe are both arranged on the inner side of the column temperature box and are both connected with connectors for connecting chromatographic columns;
The quaternary proportioning valve is also electrically connected with a controller, and the controller is also electrically connected with the single pump, the heating assembly and the temperature sensor.
The principle of the basic technical scheme is as follows: the single pump extracts a certain proportion of mobile phase from four mobile phase solvent bottles through a quaternary proportioning valve, and then conveys the mobile phase mixture to a chromatographic column in a column temperature box through a second infusion tube, a heating component maintains the interior of the column temperature box at a certain temperature, and the chromatographic column is activated, washed and balanced through the mixed mobile phase and then discharges waste liquid into a waste liquid collecting bottle.
The basic technical scheme has the beneficial effects that: after the mobile phase pipeline bubble removal preparation work is completed, the pre-maintained chromatographic column is arranged on a chromatographic column mounting frame, the proportion of the quaternary proportional valve is convenient to set under the action of a controller, the elution program and the column temperature box temperature are convenient to control on the controller, and the automation efficiency of the maintenance work of the chromatographic column is improved; in addition, under the use of the utility model, the maintenance work of the chromatographic column can be independent of the detection equipment, and the chromatographic column can be fully and effectively maintained under the condition of no detection pressure, so that the stability of the performance of the chromatographic column can be better ensured, and the service cycle of the chromatographic column can be prolonged. The chromatographic column maintenance device is independent of the chromatographic column maintenance of the detection device, so that the pollution to the device is reduced, the detection device is released from the chromatographic column maintenance work to finish more detection works, and the utilization rate of the detection device is effectively improved while the pollution to the detection device is reduced. Therefore, the design ensures that the chromatographic column can be scientifically maintained under the condition of the running load of laboratory detection equipment, ensures the service performance of the chromatographic column, effectively prolongs the service life of the chromatographic column, and improves the utilization rate of the detection equipment so as to maximize the value of the detection equipment.
Preferably, the liquid inlet end of the first infusion tube is connected with a stainless steel suction filter head.
Through the arrangement, impurities can be filtered conveniently, and the stainless steel material is convenient to clean and durable.
Preferably, the chromatographic column mounting bracket comprises a base and a tube clamp.
Through the arrangement, the pipe clamp is simple in structure, and the chromatographic column can be conveniently and rapidly fixed and detached.
Preferably, a mounting groove for accommodating the quaternary proportioning valve is formed in the side wall of the mobile phase placing box.
Through the arrangement, the installation space is saved, and the pipeline connection is convenient.
Preferably, the column temperature box is provided with a pull-out door, and a closed cavity is formed in the column temperature box through the door.
Through the arrangement, the chromatographic column is convenient to take out and put in, and the closed design can not only prevent temperature loss, but also reduce the influence of the outside on the chromatographic column.
Preferably, the controller comprises a flow phase ratio selection module, a pipeline flow speed control module, a temperature control module and a flow control module.
Through the arrangement, the automation degree of the utility model is further improved.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is an enlarged schematic view of FIG. 1 at A;
FIG. 3 is a cross-sectional view of a column oven
The names of the corresponding marks in the drawings are:
The mobile phase placing box 1, the mobile phase solvent bottle 11, the first infusion tube 12, the infusion pump box 2, the quaternary proportioning valve 21, the liquid inlet 211, the liquid outlet 212, the single pump 22, the second infusion tube 3, the column temperature box 4, the door 41, the heating component 42, the connector 43, the chromatographic column mounting frame 44, the temperature sensor 45, the waste liquid collecting bottle 5, the waste liquid tube 6 and the controller 7.
Detailed Description
The utility model is described in further detail below with reference to the attached drawings and embodiments:
As shown in fig. 1, 2 and 3, a device for maintaining a liquid chromatographic column comprises a mobile phase placing box 1, wherein four mobile phase solvent bottles 11 are placed in the mobile phase placing box 1, an infusion pump box 2 is arranged on the outer side of the mobile phase placing box 1, a single pump 22 is fixed in the infusion pump box 2, a mounting groove is formed in the side wall of the mobile phase placing box 1, a quaternary proportional valve 21 is fixed in the mounting groove, the quaternary proportional valve 21 comprises four liquid inlet ports 211 and one liquid outlet port 212, and the four liquid inlet ports 211 are connected with the four mobile phase solvent bottles 11 in a one-to-one correspondence manner through a first infusion tube 12 with a stainless steel suction filter head; the liquid inlet end of the single pump 22 is connected with the liquid outlet port 212;
The outside of the infusion pump box 2 is provided with a closed column temperature box 4 and a waste liquid collecting bottle 5, the column temperature box 4 is provided with a pull-out door 41, the column temperature box 4 is connected with a liquid outlet end of the single pump 22 through a second infusion tube 3, a heating component 42 is further arranged in the column temperature box 4, a base is fixed on the side wall of the column temperature box 4, two pipe clamps for clamping chromatographic columns are fixed on the base, and the column temperature box 4 is further provided with a temperature sensor 45 for monitoring the internal temperature of the column temperature box 4; the waste liquid collecting bottle 5 is connected with the column temperature box 4 through a waste liquid pipe 6; the liquid inlet end of the waste liquid pipe 6 and the liquid outlet end of the second infusion pipe 3 are both arranged on the inner side of the column temperature box 4 and are both connected with a joint 43 for connecting a chromatographic column;
The quaternary proportional valve 21 is also electrically connected with a controller 7, and the controller 7 is also electrically connected with the single pump 22, the heating component 42 and the temperature sensor 45; the controller 7 comprises a flow phase ratio selection module, a pipeline flow speed control module, a temperature control module and a flow control module.
The specific implementation process is as follows:
Four 1000mL mobile phase solvent bottles 11, which are respectively marked as A, B, C and D bottles, are placed in a mobile phase placing box 1, a mobile phase which is subjected to suction filtration and ultrasonic degassing is added into the bottles, a first infusion tube 12 with a stainless steel suction filter head is respectively inserted, four pipelines are respectively marked as A-1, B-1, C-1 and D-1, the other ends of the four pipelines are connected to four liquid inlet ports 211 of a quaternary proportional valve 21, a liquid outlet port 212 of the quaternary proportional valve 21 is connected with a single pump 22, bubbles in the pipelines are emptied, two ends of a chromatographic column are respectively connected with two connectors 43, the chromatographic column is clamped in a tube card of a chromatographic column mounting frame 44 after the connection is finished, and a door 41 of a column temperature box 4 is closed; after the connection is completed, presetting the working time of each mobile phase solvent bottle 11 on the quaternary proportional valve 21 through a mobile phase proportion selection module in the controller 7, and presetting the proportion of the mobile phase extracted from the four mobile phase solvent bottles 11; presetting the power of the single pump 22 through a pipeline flow rate control module, and further presetting the pipeline flow rate; the temperature in the column temperature box 4 is preset through the temperature control module, the single pump 22 and the heating component 42 are started through the flow control module, the waste liquid after the experiment is finished enters the waste liquid collecting bottle 5 through the waste liquid pipe 6, the column temperature box 4 is opened, the connector 43 is disconnected, and the chromatographic column is taken out.
The foregoing is merely exemplary embodiments of the present utility model, and detailed technical solutions or features that are well known in the art have not been described in detail herein. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present utility model, and these should also be regarded as the protection scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the practical applicability of the patent. The protection scope of the present utility model is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (6)
1. A device for maintenance of a liquid chromatography column, characterized in that: the liquid-phase solvent bottle filling device comprises a mobile phase placing box (1), wherein four mobile phase solvent bottles (11) are placed in the mobile phase placing box (1), an infusion pump box (2) is arranged on the outer side of the mobile phase placing box (1), a single pump (22) and a quaternary proportional valve (21) are fixed in the infusion pump box (2), the quaternary proportional valve (21) comprises four liquid inlet ports (211) and one liquid outlet port (212), and the four liquid inlet ports (211) are connected with the four mobile phase solvent bottles (11) in a one-to-one correspondence manner through a first infusion tube (12); the liquid inlet end of the single pump (22) is connected with the liquid outlet port (212);
The outside of the infusion pump box (2) is provided with a column temperature box (4) and a waste liquid collecting bottle (5), the column temperature box (4) is connected with a liquid outlet end of the single pump (22) through a second infusion tube (3), a heating component (42) and a chromatographic column mounting frame (44) are further arranged in the column temperature box (4), and a temperature sensor (45) for monitoring the internal temperature of the column temperature box (4) is further arranged on the column temperature box (4); the waste liquid collecting bottle (5) is connected with the column temperature box (4) through a waste liquid pipe (6); the liquid inlet end of the waste liquid pipe (6) and the liquid outlet end of the second infusion pipe (3) are both arranged at the inner side of the column temperature box (4), and are both connected with connectors (43) for connecting chromatographic columns;
The quaternary proportional valve (21) is further electrically connected with a controller (7), and the controller (7) is also electrically connected with the single pump (22), the heating component (42) and the temperature sensor (45).
2. The liquid chromatography column maintenance device according to claim 1, wherein: the liquid inlet end of the first infusion tube (12) is connected with a stainless steel suction filter head.
3. The liquid chromatography column maintenance device according to claim 1, wherein: the chromatographic column mounting bracket (44) includes a base and a tube clamp.
4. The liquid chromatography column maintenance device according to claim 1, wherein: the side wall of the mobile phase placing box (1) is provided with a mounting groove for accommodating the quaternary proportional valve (21).
5. The liquid chromatography column maintenance device according to claim 1, wherein: the column temperature box (4) is provided with a pull-out door (41), and a closed cavity is formed in the column temperature box (4) through the door (41).
6. The liquid chromatography column maintenance device according to claim 1, wherein: the controller (7) comprises a flow phase ratio selection module, a pipeline flow speed control module, a temperature control module and a flow control module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322779701.7U CN220860715U (en) | 2023-10-17 | 2023-10-17 | Device for maintaining liquid chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322779701.7U CN220860715U (en) | 2023-10-17 | 2023-10-17 | Device for maintaining liquid chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220860715U true CN220860715U (en) | 2024-04-30 |
Family
ID=90822436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322779701.7U Active CN220860715U (en) | 2023-10-17 | 2023-10-17 | Device for maintaining liquid chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220860715U (en) |
-
2023
- 2023-10-17 CN CN202322779701.7U patent/CN220860715U/en active Active
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